##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938613_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3240411 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.842193474840073 31.0 31.0 34.0 30.0 34.0 2 32.07167053808915 33.0 31.0 34.0 30.0 34.0 3 32.13334080152178 33.0 31.0 34.0 30.0 34.0 4 35.804549484617844 37.0 35.0 37.0 35.0 37.0 5 35.67470144990867 37.0 35.0 37.0 33.0 37.0 6 35.717018921365224 37.0 35.0 37.0 33.0 37.0 7 35.64981355760118 37.0 35.0 37.0 33.0 37.0 8 35.629525699054845 37.0 35.0 37.0 33.0 37.0 9 37.206874992092054 39.0 37.0 39.0 33.0 39.0 10-11 37.14235617025125 39.0 37.0 39.0 33.0 39.0 12-13 37.04431397745533 39.0 37.0 39.0 33.0 39.0 14-15 38.10543029881086 40.0 37.0 41.0 33.0 41.0 16-17 38.040115435974016 40.0 37.0 41.0 33.0 41.0 18-19 38.05232407247105 40.0 37.0 41.0 33.0 41.0 20-21 38.03412360345648 40.0 37.0 41.0 33.0 41.0 22-23 38.06443565337854 40.0 37.0 41.0 33.0 41.0 24-25 37.98855870443595 40.0 37.0 41.0 33.0 41.0 26-27 37.81292913152066 40.0 37.0 41.0 32.0 41.0 28-29 37.68701902320416 40.0 37.0 41.0 32.0 41.0 30-31 37.43011272335515 39.0 36.5 41.0 32.0 41.0 32-33 37.19760795775598 39.0 36.0 41.0 31.0 41.0 34-35 37.03559440453695 39.0 36.0 41.0 31.0 41.0 36-37 37.02549522267391 39.0 36.0 41.0 31.0 41.0 38-39 36.8638774525824 39.0 35.0 41.0 30.5 41.0 40-41 36.67651757755421 39.0 35.0 41.0 30.0 41.0 42-43 36.430786557631116 39.0 35.0 40.5 30.0 41.0 44-45 36.14294467584513 39.0 35.0 40.0 30.0 41.0 46-47 35.76674208919795 38.0 35.0 40.0 28.5 41.0 48-49 35.35010975459595 38.0 34.5 40.0 27.0 41.0 50-51 34.968678047321774 38.0 34.0 40.0 26.0 41.0 52-53 34.45433650237578 38.0 33.0 40.0 24.5 41.0 54-55 34.13058081212537 37.5 33.0 40.0 24.0 41.0 56-57 33.767049149012266 37.0 33.0 40.0 23.0 41.0 58-59 33.316617398225105 36.5 32.0 39.0 22.5 41.0 60-61 32.91704524518649 36.0 31.5 39.0 21.5 40.5 62-63 32.45545981667141 35.5 31.0 39.0 20.0 40.0 64-65 31.966545601777057 35.0 31.0 38.5 18.0 40.0 66-67 31.489125916434674 35.0 30.0 38.0 17.0 40.0 68-69 30.94520787640827 34.5 29.5 37.5 12.5 39.5 70-71 30.38880793208022 34.0 29.0 37.0 8.0 39.0 72-73 29.836783358654195 34.0 29.0 36.0 7.0 39.0 74-75 29.26830562542838 34.0 28.0 35.5 6.0 38.5 76-77 28.0043198223929 31.5 26.5 34.5 2.0 36.0 78-79 28.64775486813247 33.0 28.0 35.0 2.0 37.0 80-81 28.406263742469704 33.0 27.0 35.0 2.0 36.5 82-83 27.953595392683212 33.0 26.5 35.0 2.0 36.0 84-85 27.416026392948304 33.0 26.0 35.0 2.0 36.0 86-87 26.84649015202084 32.5 25.0 35.0 2.0 35.0 88-89 26.134721953480593 32.0 24.0 34.0 2.0 35.0 90-91 25.647333779573025 32.0 22.5 34.0 2.0 35.0 92-93 24.934458005481403 31.0 19.5 34.0 2.0 35.0 94-95 24.212300538419356 31.0 14.0 34.0 2.0 35.0 96-97 23.305613855773235 31.0 2.0 34.0 2.0 35.0 98-99 22.18663404117564 30.0 2.0 34.0 2.0 35.0 100-101 19.020790572553913 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22059934378502177 1101 2 0.24006836467490444 1101 3 0.23510620289137307 1101 4 0.1802873442343227 1101 5 0.13523494734527475 1101 6 0.2141685680445775 1101 7 0.23304183300675874 1101 8 0.28095709089301835 1101 9 0.35873256131061737 1101 10-11 0.3433774785142205 1101 12-13 0.29919107936242284 1101 14-15 0.40870731633292934 1101 16-17 0.48627624467862773 1101 18-19 0.5065930965516401 1101 20-21 0.4921015898363805 1101 22-23 0.4678093915305226 1101 24-25 0.5215172756185567 1101 26-27 0.5123477334212794 1101 28-29 0.4093748352835789 1101 30-31 0.48551317187747856 1101 32-33 0.3731531026242365 1101 34-35 0.34178858343752694 1101 36-37 0.43730305222705823 1101 38-39 0.6155274644004081 1101 40-41 0.612298129693194 1101 42-43 0.5612167614010062 1101 44-45 0.5984596556725492 1101 46-47 0.9186194569973978 1101 48-49 0.961468700909208 1101 50-51 0.961439758860628 1101 52-53 0.9470984645522051 1101 54-55 0.9917719435823287 1101 56-57 1.139869813607696 1101 58-59 1.0344377089650791 1101 60-61 1.1287122913734606 1101 62-63 1.2172975515616429 1101 64-65 1.2826455564253365 1101 66-67 1.3772487651112755 1101 68-69 1.284471485834075 1101 70-71 1.3110459354271917 1101 72-73 1.3432926974028696 1101 74-75 1.35687470260277 1101 76-77 1.1295627313142873 1101 78-79 1.420048220866036 1101 80-81 1.7094530914585242 1101 82-83 1.892469369100798 1101 84-85 2.100202713311038 1101 86-87 2.097001266146467 1101 88-89 2.010764526385742 1101 90-91 2.2264520517877386 1101 92-93 2.5808972713617244 1101 94-95 2.5719456563829795 1101 96-97 2.8257157867440625 1101 98-99 3.2426810602705523 1101 100-101 3.8217747242325757 1102 1 0.27573280707327186 1102 2 0.264428231369628 1102 3 0.22426043995349687 1102 4 0.16304596492398105 1102 5 0.07674264533703479 1102 6 0.11074726977491878 1102 7 0.219624463188417 1102 8 0.1820071358685169 1102 9 0.35862837641801804 1102 10-11 0.34954956626385325 1102 12-13 0.24700144949755298 1102 14-15 0.4029566571161922 1102 16-17 0.3349088918304801 1102 18-19 0.38899520193434256 1102 20-21 0.39751680196118855 1102 22-23 0.2883457754386214 1102 24-25 0.4041818344360024 1102 26-27 0.3965354984921845 1102 28-29 0.26251627314815096 1102 30-31 0.36880971291335385 1102 32-33 0.186035429706223 1102 34-35 0.2940207736660341 1102 36-37 0.5154251497271574 1102 38-39 0.5720893012815438 1102 40-41 0.5252043126747523 1102 42-43 0.42281869340770584 1102 44-45 0.40043237284795197 1102 46-47 0.41382875044398304 1102 48-49 0.47686140858335335 1102 50-51 0.5025398639704619 1102 52-53 0.6593864881198286 1102 54-55 0.5487475268057622 1102 56-57 0.6506977841810482 1102 58-59 0.6280723074961116 1102 60-61 0.5768354731158496 1102 62-63 0.7660567236219009 1102 64-65 0.652110171972808 1102 66-67 0.7733585063601964 1102 68-69 0.8024179437918093 1102 70-71 0.8756517452701083 1102 72-73 0.8416501474191698 1102 74-75 1.0752766193992507 1102 76-77 0.9531193209252393 1102 78-79 1.0748292668720794 1102 80-81 1.3566697490170405 1102 82-83 1.5206869287386233 1102 84-85 1.5890297507166231 1102 86-87 1.529494915492748 1102 88-89 1.5077277487332594 1102 90-91 1.7755458102262658 1102 92-93 2.0261748951959397 1102 94-95 2.2020222932997875 1102 96-97 2.570468837717069 1102 98-99 2.9688309416076883 1102 100-101 3.630186543090119 1103 1 0.28740357302390507 1103 2 0.29698444265578416 1103 3 0.3001587405506214 1103 4 0.18643278636474037 1103 5 0.1726589223975381 1103 6 0.16478814184242196 1103 7 0.2139388840745582 1103 8 0.23772023708716006 1103 9 0.2839389581447378 1103 10-11 0.32450793472560946 1103 12-13 0.3917659787302128 1103 14-15 0.5194918679912561 1103 16-17 0.5327388261258008 1103 18-19 0.4022929074965944 1103 20-21 0.4529355211730177 1103 22-23 0.3883851424998852 1103 24-25 0.39815832568118736 1103 26-27 0.4738830643536289 1103 28-29 0.3018903020359076 1103 30-31 0.41526807092368045 1103 32-33 0.4669960556773347 1103 34-35 0.49038576554875846 1103 36-37 0.6093103651311722 1103 38-39 0.7856085772063253 1103 40-41 0.6015206705596654 1103 42-43 0.6605591634434447 1103 44-45 0.6773517783704861 1103 46-47 0.734955972244272 1103 48-49 0.7872044942641949 1103 50-51 0.7141047220748575 1103 52-53 0.9259450405267984 1103 54-55 0.9709313884009347 1103 56-57 0.8652918157165033 1103 58-59 1.1619384316173225 1103 60-61 1.1336546842417476 1103 62-63 1.146286794482613 1103 64-65 0.8752465074219771 1103 66-67 1.0000692316199036 1103 68-69 1.0888307335577636 1103 70-71 1.1867212054884746 1103 72-73 1.1953266128841555 1103 74-75 1.2928353738672378 1103 76-77 1.107593230666673 1103 78-79 1.3683246438552388 1103 80-81 1.7077246469065486 1103 82-83 1.865504063513324 1103 84-85 2.0021783491795695 1103 86-87 2.1378396873379835 1103 88-89 2.4165947438071527 1103 90-91 2.616187597839229 1103 92-93 2.6936068389533006 1103 94-95 2.524579619405376 1103 96-97 2.8089078118044846 1103 98-99 3.39118899373943 1103 100-101 3.8344458500904857 1104 1 0.1889261197885581 1104 2 0.18194832470641842 1104 3 0.1747845334384408 1104 4 0.1519978705501117 1104 5 0.04504980486146337 1104 6 0.12036452411767584 1104 7 0.13526532025719717 1104 8 0.20982159195948924 1104 9 0.21000643671654728 1104 10-11 0.19360698505713714 1104 12-13 0.26084571224803454 1104 14-15 0.23430179952887187 1104 16-17 0.3191454607774773 1104 18-19 0.4418154452766814 1104 20-21 0.39770072081003605 1104 22-23 0.31134952094771506 1104 24-25 0.3155653578998354 1104 26-27 0.30639107184674685 1104 28-29 0.28037642537601215 1104 30-31 0.35328737875283167 1104 32-33 0.38198736311115766 1104 34-35 0.42611698556277133 1104 36-37 0.4622868390489856 1104 38-39 0.4019357770769503 1104 40-41 0.42345772020836847 1104 42-43 0.5165070013079998 1104 44-45 0.4786242734739048 1104 46-47 0.4616439473031235 1104 48-49 0.6273510100500772 1104 50-51 0.5298249023052222 1104 52-53 0.6925477857604889 1104 54-55 0.7408930692332589 1104 56-57 0.8223292566429947 1104 58-59 1.0422317439567266 1104 60-61 0.8940479161692991 1104 62-63 0.9880711963801545 1104 64-65 0.8391096904734923 1104 66-67 1.000721904078631 1104 68-69 1.0542056257429877 1104 70-71 1.0696465460034723 1104 72-73 1.279712899743334 1104 74-75 1.4362697348850126 1104 76-77 1.3389118502781798 1104 78-79 1.534859499473285 1104 80-81 1.6372485436020234 1104 82-83 1.7162515792812343 1104 84-85 1.8512508052001238 1104 86-87 1.9614261907251667 1104 88-89 1.9924511412663186 1104 90-91 2.1827239696025416 1104 92-93 2.5679108159707837 1104 94-95 2.5784384337123925 1104 96-97 2.935357583596545 1104 98-99 3.2746467820091816 1104 100-101 3.7111890802211214 1105 1 0.311459747380777 1105 2 0.3094107187564745 1105 3 0.23898433912707873 1105 4 0.19460943207646153 1105 5 0.10704266894401115 1105 6 0.206168278012278 1105 7 0.28094817395413685 1105 8 0.2655072933230187 1105 9 0.3037267037271718 1105 10-11 0.3316804967325666 1105 12-13 0.38654611259645577 1105 14-15 0.49909752132859353 1105 16-17 0.5247219666905707 1105 18-19 0.45342701517852646 1105 20-21 0.42105378540180993 1105 22-23 0.3302807456118586 1105 24-25 0.3779709242504623 1105 26-27 0.5652134988962345 1105 28-29 0.5930454008860764 1105 30-31 0.6103717869407106 1105 32-33 0.565915007253345 1105 34-35 0.5402268594278965 1105 36-37 0.6955649639442925 1105 38-39 0.6612736108402189 1105 40-41 0.5345500097323068 1105 42-43 0.5958326277946497 1105 44-45 0.6271569923254532 1105 46-47 0.6851325901387284 1105 48-49 0.6746597557366982 1105 50-51 0.7881842694531969 1105 52-53 0.6854461031245549 1105 54-55 0.7035429905036565 1105 56-57 0.8688225436877843 1105 58-59 0.9789512529855031 1105 60-61 0.861761030511488 1105 62-63 1.0681582595983485 1105 64-65 0.9901348504453509 1105 66-67 1.0056679449845234 1105 68-69 1.0507805738149472 1105 70-71 1.1098946489549952 1105 72-73 1.2821101743588947 1105 74-75 1.3214569480244798 1105 76-77 1.2768029856596002 1105 78-79 1.5631169369083757 1105 80-81 1.8224556485301626 1105 82-83 1.8733992224183602 1105 84-85 1.9568190403168764 1105 86-87 2.162401516531723 1105 88-89 2.2791959861522457 1105 90-91 2.368567821155356 1105 92-93 2.6458473674465424 1105 94-95 2.6285191524969456 1105 96-97 2.9505065416855913 1105 98-99 3.298504260484176 1105 100-101 3.876274593973676 1106 1 0.13480929363226224 1106 2 0.15208389089461605 1106 3 0.14192966900463233 1106 4 0.08618846066175223 1106 5 0.08743417487741567 1106 6 0.0691982561750919 1106 7 0.07125016874203993 1106 8 0.10932318685581777 1106 9 0.0902596265092086 1106 10-11 0.11014406639684893 1106 12-13 0.051984069504811714 1106 14-15 0.03368377720032356 1106 16-17 0.09607428852866917 1106 18-19 0.1755175983867332 1106 20-21 0.25706575270795895 1106 22-23 0.1725287474229873 1106 24-25 0.12701870718213115 1106 26-27 0.11459980390417002 1106 28-29 0.27107618614156337 1106 30-31 0.17782884605745153 1106 32-33 0.15027883041579315 1106 34-35 0.16131933803486476 1106 36-37 0.295311161218045 1106 38-39 0.14336320929385238 1106 40-41 0.04192861335031495 1106 42-43 0.2214461958853562 1106 44-45 -0.25513270419754974 1106 46-47 -0.34906179432366713 1106 48-49 0.026085061086760675 1106 50-51 -0.023195432623005274 1106 52-53 0.12275611909381468 1106 54-55 0.17065782202433155 1106 56-57 0.08414644165097229 1106 58-59 0.5922267599056852 1106 60-61 0.354424710427665 1106 62-63 0.39166968122864176 1106 64-65 0.05019421997907614 1106 66-67 0.15164395352041282 1106 68-69 -0.049826471545685536 1106 70-71 0.15014395429693295 1106 72-73 0.564910268164386 1106 74-75 0.8767551814559837 1106 76-77 0.9057180703738723 1106 78-79 1.03193884556579 1106 80-81 1.0302559199975612 1106 82-83 1.3562037323912826 1106 84-85 1.4079792740996453 1106 86-87 1.370975632511449 1106 88-89 1.6649640997990147 1106 90-91 1.7877229727923307 1106 92-93 1.3520565496230965 1106 94-95 1.8746954113838434 1106 96-97 2.2995421928469426 1106 98-99 2.6984755300155605 1106 100-101 3.3545104518319633 1107 1 0.15857881256701845 1107 2 0.15670352666480625 1107 3 0.15232431312020367 1107 4 0.11296788279001646 1107 5 0.12372177548569852 1107 6 0.12483973831596273 1107 7 0.14547033861704506 1107 8 0.15793970701456317 1107 9 0.18161751382793057 1107 10-11 0.19636861296411467 1107 12-13 0.2692988946225725 1107 14-15 0.3478727909609418 1107 16-17 0.33518355747270334 1107 18-19 0.34523496792050423 1107 20-21 0.3778606045309658 1107 22-23 0.31909508398320696 1107 24-25 0.33902772020093863 1107 26-27 0.35855217955788987 1107 28-29 0.3628504155840844 1107 30-31 0.38558542036849275 1107 32-33 0.4148591501368699 1107 34-35 0.3638962083289883 1107 36-37 0.31943953182744167 1107 38-39 0.2988758015567541 1107 40-41 0.4284072306122866 1107 42-43 0.5037660282357805 1107 44-45 0.6495277312462377 1107 46-47 0.5191561872052048 1107 48-49 0.4953666159252279 1107 50-51 0.4133851838229674 1107 52-53 0.5626174002035711 1107 54-55 0.596661123088829 1107 56-57 0.5961090914043154 1107 58-59 0.5733096203337098 1107 60-61 0.7070757619465269 1107 62-63 0.8916743175551858 1107 64-65 0.9637730931662674 1107 66-67 1.0852499028546418 1107 68-69 1.1594879084847278 1107 70-71 1.0019484125885363 1107 72-73 1.200895580281884 1107 74-75 1.1672405121187914 1107 76-77 1.0509222541949477 1107 78-79 1.2844693525944635 1107 80-81 1.4379179132470696 1107 82-83 1.5910679807500223 1107 84-85 1.8325926044657272 1107 86-87 2.039899262940583 1107 88-89 2.1877349539958146 1107 90-91 2.2322687982439113 1107 92-93 2.473147199079719 1107 94-95 2.536956640566739 1107 96-97 2.870310153975982 1107 98-99 3.174918354836599 1107 100-101 3.4422631316287102 1108 1 0.09761328956734161 1108 2 0.1389019800691358 1108 3 0.07247827628528114 1108 4 0.06530372791022643 1108 5 0.024299369792558423 1108 6 -1.716101187199115E-4 1108 7 0.10531082611298359 1108 8 0.09290387877572215 1108 9 0.14402402369113787 1108 10-11 0.11512202433092256 1108 12-13 0.22235534255704437 1108 14-15 0.26421513850634426 1108 16-17 0.23289045356552407 1108 18-19 0.2099653637502641 1108 20-21 0.25368639685224537 1108 22-23 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-0.3156338933496059 2316 54-55 -0.181467318290494 2316 56-57 -0.10210040553856459 2316 58-59 -0.09691004440936268 2316 60-61 0.07816417671543974 2316 62-63 0.10659967609463905 2316 64-65 0.16722020448260366 2316 66-67 0.19023186736368913 2316 68-69 0.1791806277941035 2316 70-71 0.4259926492291868 2316 72-73 0.40892033229587454 2316 74-75 0.5202472461319516 2316 76-77 0.6492301489476908 2316 78-79 0.6323751734008951 2316 80-81 0.6621961120066722 2316 82-83 0.9265803044456682 2316 84-85 1.1063070569948472 2316 86-87 1.26973713273334 2316 88-89 1.414507322981322 2316 90-91 1.638848039764305 2316 92-93 1.5319101373939858 2316 94-95 1.9084568680972644 2316 96-97 2.21059918770424 2316 98-99 2.452595471689346 2316 100-101 2.9532528083254803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 39.0 5 108.0 6 226.0 7 623.0 8 1401.0 9 2805.0 10 4285.0 11 6879.0 12 9745.0 13 13341.0 14 17477.0 15 22946.0 16 29089.0 17 36610.0 18 34048.0 19 28676.0 20 28239.0 21 29733.0 22 32394.0 23 36495.0 24 42442.0 25 49446.0 26 58606.0 27 70039.0 28 84032.0 29 100517.0 30 120111.0 31 142993.0 32 172454.0 33 211470.0 34 267106.0 35 347106.0 36 449311.0 37 501975.0 38 270806.0 39 16818.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03698793485265 18.73082031645398 14.906949989013729 23.325241759679646 2 16.14995333293608 20.274037397252958 38.67388969283099 24.90211957697997 3 19.507931878885508 23.62544188149321 29.745880530365802 27.120745709255477 4 13.556305048958295 16.55499873318539 35.47046964104245 34.418226576813865 5 13.848468822247954 37.44076517492731 33.687269779643366 15.023496223181375 6 33.413847811280725 35.60684123094262 16.37619425437082 14.603116703405833 7 29.568131943756516 32.68239738724502 19.52172116438316 18.22774950461531 8 27.564805822471282 34.09752651746955 19.10452100057678 19.233146659482394 9 26.994476935178902 16.262751854625847 19.338040760878787 37.40473044931646 10-11 25.50008316846227 26.25699332584663 26.513149103616797 21.729774402074305 12-13 26.6050664560761 23.486449712706197 26.91306750902895 22.99541632218876 14-15 22.929406177179377 24.882121434595796 25.335937323999953 26.852535064224874 16-17 22.525985129664107 28.144547096031953 26.423499981946737 22.905967792357202 18-19 22.72619121463296 26.26885910460124 28.87170794075196 22.133241740013844 20-21 22.939451199246022 25.38537549712058 29.34681742532043 22.328355878312966 22-23 22.918944541294298 25.408551569538556 29.214596543463163 22.457907345703987 24-25 22.76691238339154 25.681273318027536 29.18432982258741 22.367484475993514 26-27 21.97944085406889 26.183752710013824 28.95539335380925 22.881413082108036 28-29 22.385910305822318 26.560365336372456 28.704692089984878 22.34903226782035 30-31 22.25858077879627 26.018859953259017 28.525717879614653 23.19684138833006 32-33 21.81505453196572 26.024698238763015 28.670843320547846 23.489403908723418 34-35 23.025776672134867 26.136060951651956 28.334339008841198 22.50382336737198 36-37 23.269228899752385 25.414377599190697 28.38241014791325 22.933983353143674 38-39 22.407971015163707 25.844658042696402 28.40146368608526 23.345907256054634 40-41 23.097045344387823 25.69442269075763 28.496664471837548 22.711867493017003 42-43 22.682462772934187 25.822513694930947 28.222235938584983 23.27278759354988 44-45 22.52064180510038 26.14454795415096 28.624542658393224 22.710267582355435 46-47 23.303442545420598 26.543714023622027 27.500954741031798 22.651888689925574 48-49 22.76865496383021 26.636065610195743 27.384550910362915 23.210728515611137 50-51 23.20022058930179 26.708186091208802 27.42561051669063 22.66598280279878 52-53 23.668093337542675 26.140449467675552 27.49441351729765 22.69704367748412 54-55 22.708477412278874 26.733090339466198 27.718181428220063 22.840250820034864 56-57 22.83159451069633 26.842767784703852 27.396864163218805 22.928773541381016 58-59 22.730280202110166 26.23551456898523 27.37233023835557 23.66187499054904 60-61 22.118305363115976 26.6558007610763 27.857237862727906 23.36865601307982 62-63 22.464588596940327 26.720792516751736 27.977268932860678 22.837349953447266 64-65 22.105945819835817 26.786062014972796 27.591885720669385 23.516106444522006 66-67 21.978801116464865 26.72761889596259 27.68282312728357 23.61075686028898 68-69 22.863820592823952 26.292820451020717 27.707365636800702 23.135993319354633 70-71 22.580684981010123 26.46875658674162 27.162866068532665 23.78769236371559 72-73 22.4414931379836 26.687189786602655 27.183222619479846 23.6880944559339 74-75 22.412558160060563 27.097010224937517 27.00084958358677 23.48958203141515 76-77 22.57037764653928 26.506467852380457 27.36868872497964 23.554465776100624 78-79 22.65342266768012 26.761065185866855 27.350218845695807 23.235293300757217 80-81 22.830416958103598 26.757142885798846 27.012558135013492 23.399882021084064 82-83 22.621523443370737 26.859695289106256 27.187588627640626 23.331192639882385 84-85 22.40036885735386 27.02498891298491 27.141599313885816 23.43304291577541 86-87 22.78118907915848 27.060711837803858 26.609560072250943 23.54853901078672 88-89 22.449990109273244 27.18048863584813 26.468911794776524 23.900609460102103 90-91 22.31202796948144 26.984075060123953 26.75160297112476 23.95229399926984 92-93 22.361824048927197 26.6043164646164 27.00647279901457 24.027386687441837 94-95 22.11128464876832 26.27054099001639 27.24746953395727 24.370704827258024 96-97 22.36625230564888 25.86522203510604 27.218260276242734 24.550265383002344 98-99 21.985991900410166 26.282206176932494 26.851084630931076 24.880717291726263 100-101 22.00209573894703 25.87316412604739 27.345257947012154 24.77948218799343 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 599.0 1 422.0 2 360.0 3 479.0 4 425.0 5 450.0 6 809.5 7 1744.0 8 2759.5 9 3145.5 10 2789.0 11 2398.5 12 2417.5 13 2535.5 14 2772.5 15 2983.5 16 3167.5 17 3500.5 18 3936.0 19 4400.5 20 4969.0 21 5748.5 22 6955.5 23 8433.5 24 10094.5 25 12665.0 26 16199.0 27 20287.0 28 24220.5 29 28238.5 30 32883.0 31 41159.5 32 55247.0 33 58904.0 34 54181.0 35 56419.5 36 62763.0 37 69741.0 38 75317.5 39 79338.5 40 84288.0 41 91560.0 42 95690.5 43 99834.0 44 105068.0 45 111706.5 46 116841.5 47 121835.0 48 123092.5 49 119871.5 50 120128.0 51 118650.5 52 116265.5 53 119237.0 54 125425.0 55 119310.5 56 107385.0 57 98748.0 58 92720.0 59 82804.0 60 65931.5 61 55343.5 62 48624.5 63 35942.0 64 24363.0 65 18369.0 66 13532.5 67 10437.5 68 8322.0 69 6557.0 70 5002.5 71 3286.5 72 2328.5 73 1896.5 74 1406.5 75 1015.0 76 702.5 77 497.5 78 335.5 79 195.5 80 114.5 81 70.5 82 45.5 83 28.0 84 15.5 85 9.0 86 6.0 87 4.5 88 4.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.25808485405092E-5 2 0.046599027098722975 3 1.851616970810184E-4 4 0.0 5 0.10168463198032594 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.177974954411646E-4 26-27 0.010631367440735142 28-29 0.0 30-31 0.0 32-33 5.554850912430553E-4 34-35 0.014380891806625764 36-37 0.0030705981432602226 38-39 0.011480025219023143 40-41 0.006773832084880591 42-43 0.004968505538340661 44-45 0.002515113052017167 46-47 1.0801098996392742E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.3887127281076383E-4 68-69 1.6973155565760023E-4 70-71 0.0 72-73 1.5430141423418203E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.62904242702546E-5 82-83 8.640879197114194E-4 84-85 0.004135277901476078 86-87 0.0011726907481797832 88-89 6.172056569367281E-5 90-91 6.789262226304009E-4 92-93 1.3887127281076383E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.177974954411646E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3240411.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.661013845970494 #Duplication Level Percentage of deduplicated Percentage of total 1 68.92005761531827 28.023594169615407 2 15.133763152951468 12.307083062075954 3 6.084576134770085 7.422151033882442 4 3.0569895522388397 4.972011780422824 5 1.716698748772693 3.490135579660335 6 1.0577364588246894 2.5805182078595097 7 0.7685509350094322 2.1875042146826464 8 0.5341246208952667 1.7374438884556944 9 0.3927735317176697 1.437351301035261 >10 2.068827864914496 15.300900952354437 >50 0.15888579729418784 4.469960671346002 >100 0.09233846841315296 7.100963257999158 >500 0.008593339033664698 2.3610483207687603 >1k 0.00539935461369153 4.687649998853178 >5k 6.083779846412992E-4 1.5937292615396754 >10k+ 7.60472480801624E-5 0.3279542994487844 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10606 0.32730415987354694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8852 0.2731752237601959 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6669 0.20580722630555198 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6655 0.20537518234569627 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6414 0.1979378541796087 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6137 0.189389555831035 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5837 0.1801314709769841 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5663 0.17476178176163457 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5314 0.16399154304808863 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4893 0.15099936396957053 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4449 0.13729739838557517 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4309 0.13297695878701807 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4200 0.1296131879567129 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3966 0.12239188177055318 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3855 0.11896639037455434 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3629 0.11199196645116931 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3541 0.1092762615606477 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3522 0.10868991618655781 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3435 0.10600507157888304 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3288 0.10146861000039809 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.629042427025461E-5 0.0 12-13 0.0 0.0 0.0 1.0801098996392742E-4 0.0 14-15 0.0 0.0 0.0 1.2344113138734562E-4 0.0 16-17 0.0 0.0 0.0 1.5430141423418203E-4 0.0 18-19 0.0 0.0 0.0 1.8516169708101844E-4 0.0 20-21 0.0 0.0 0.0 1.8516169708101844E-4 0.0 22-23 0.0 0.0 0.0 4.937645255493825E-4 0.0 24-25 0.0 0.0 0.0 0.001064679758215856 1.5430141423418203E-5 26-27 0.0 0.0 0.0 0.002206510223548803 3.0860282846836406E-5 28-29 0.0 0.0 0.0 0.005415979639619789 3.0860282846836406E-5 30-31 0.0 0.0 0.0 0.01691143500006635 3.0860282846836406E-5 32-33 0.0 0.0 0.0 0.04127562830764369 3.0860282846836406E-5 34-35 0.0 0.0 0.0 0.07782963333972141 3.0860282846836406E-5 36-37 0.0 0.0 0.0 0.13896385365930433 3.0860282846836406E-5 38-39 0.0 0.0 0.0 0.22935362211768817 3.0860282846836406E-5 40-41 0.0 0.0 0.0 0.32796765595475386 3.0860282846836406E-5 42-43 0.0 0.0 0.0 0.42747663799437785 3.0860282846836406E-5 44-45 0.0 0.0 0.0 0.5533403015852001 3.0860282846836406E-5 46-47 0.0 0.0 0.0 0.7030589638166269 3.0860282846836406E-5 48-49 0.0 0.0 0.0 0.86178882863933 3.0860282846836406E-5 50-51 0.0 0.0 0.0 1.0382016355332704 3.0860282846836406E-5 52-53 0.0 0.0 0.0 1.2033658693295388 3.0860282846836406E-5 54-55 0.0 0.0 0.0 1.3800564187691005 3.0860282846836406E-5 56-57 0.0 0.0 0.0 1.5870363358228323 3.0860282846836406E-5 58-59 0.0 0.0 0.0 1.8106962357552792 3.0860282846836406E-5 60-61 0.0 0.0 0.0 2.060371354127609 3.0860282846836406E-5 62-63 0.0 0.0 0.0 2.303473232253563 3.0860282846836406E-5 64-65 0.0 0.0 0.0 2.5524848545446854 3.0860282846836406E-5 66-67 0.0 0.0 0.0 2.846814802196388 3.0860282846836406E-5 68-69 0.0 0.0 0.0 3.1708323419467472 3.0860282846836406E-5 70-71 0.0 0.0 0.0 3.503783933581265 3.0860282846836406E-5 72-73 0.0 0.0 0.0 3.8602973511693426 3.0860282846836406E-5 74-75 0.0 0.0 0.0 4.218554374738266 3.0860282846836406E-5 76-77 0.0 0.0 0.0 4.598459886724246 3.0860282846836406E-5 78-79 0.0 3.0860282846836406E-5 0.0 5.030858739832694 3.0860282846836406E-5 80-81 0.0 3.0860282846836406E-5 0.0 5.490275770573548 3.0860282846836406E-5 82-83 0.0 3.0860282846836406E-5 0.0 5.968749025972323 3.0860282846836406E-5 84-85 0.0 3.0860282846836406E-5 0.0 6.453672080486086 3.0860282846836406E-5 86-87 0.0 3.0860282846836406E-5 0.0 6.9494270942790894 3.0860282846836406E-5 88-89 0.0 3.0860282846836406E-5 0.0 7.473928461543922 3.0860282846836406E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5630 0.0 59.652756 1 GTATCAA 10975 0.0 44.64439 1 TGGTATC 2205 0.0 33.007835 2 ATCAACG 14770 0.0 32.863293 3 TATCAAC 14825 0.0 32.857876 2 TCAACGC 14925 0.0 32.490147 4 CAACGCA 15155 0.0 32.02843 5 AACGCAG 15735 0.0 30.848516 6 TAAGGTG 2535 0.0 28.693222 5 AGGTAAG 2400 0.0 26.9564 2 GTAAGGT 2640 0.0 26.651619 4 ACGCAGA 18240 0.0 26.507765 7 GGTAAGG 2650 0.0 26.371647 3 CGCAGAG 18215 0.0 26.283396 8 AAGGTAA 2430 0.0 25.840557 1 GTGGTAT 3270 0.0 24.439693 1 TACATGG 13405 0.0 23.06645 2 GCAGAGT 20670 0.0 22.862974 9 GTACATG 13915 0.0 22.631275 1 TAAGGTA 1290 0.0 22.4805 4 >>END_MODULE