FastQCFastQC Report
Thu 26 May 2016
SRR938612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250792
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106630.3280123736000335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82920.25507630140593435No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65740.20222764175622435No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65440.20130478972508853No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA62220.19139951125756433No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA55340.17023543801018337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54460.16752840538551836No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52350.16103767943319658No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47270.14541071837263042No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA46870.1441802489977827No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46450.1428882561541926No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43060.13246002820235808No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41900.12889166701529967No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT38850.11950933803208572No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35200.10828130498660019No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA34820.10711235908049484No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33900.10428227951834507No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG33250.10228276678421751No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT32550.10012944537823398No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58950.060.601481
GTATCAA111200.044.3507541
TATCAAC150550.032.4867322
ATCAACG150850.032.3906173
TCAACGC154450.031.6048684
CAACGCA155150.031.4000345
TAAGGTG23300.031.0059975
AGGTAAG21800.030.5241032
AACGCAG160450.030.3854276
TGGTATC23850.029.893332
GTAAGGT23750.029.8190674
GGTAAGG25400.027.1334933
CGCAGAG187200.025.7829158
ACGCAGA188200.025.7468817
GTGTAAG14600.025.4816151
AAGGTAA23800.025.2510131
TACATGG144800.024.9140662
GTACATG148850.024.8014661
GTACCGT2900.024.5782556
TAAGGTA12250.024.05624
Produced by FastQC (version 0.11.5)