FastQCFastQC Report
Thu 26 May 2016
SRR938612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250792
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102540.3154308242422154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85500.26301282887370214No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65200.20056650810017987No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA64660.19890537444413547No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62160.19121494085133714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59610.18337069858668287No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC57480.17681844916561873No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56690.17438827215029445No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG52070.16017635087080317No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46320.14248835360736709No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA43660.13430573226462966No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA41970.12910699915589802No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41380.1272920568279976No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA39030.1200630492507672No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT37890.11655621153245116No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT36120.11111138454874996No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34990.10763530856480513No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG34610.1064663626586998No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33940.10440532645582982No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA33510.10308257187786854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56450.059.3466871
GTATCAA108800.045.5977021
TGGTATC21950.035.0621222
TATCAAC148800.033.2357522
ATCAACG149200.032.9500273
TCAACGC152300.032.309544
CAACGCA154650.031.8492895
AACGCAG158850.031.0071956
TAAGGTG23050.028.6427545
AGGTAAG22150.027.8822212
ACGCAGA184150.026.6697967
CGCAGAG183950.026.6471528
GTAAGGT25950.025.8078984
GTGGTAT32150.025.4225331
TACATGG142000.024.5229852
GTACATG144150.024.2954081
GGTAAGG24800.024.1325443
AAGGTAA23700.023.8599741
GCAGAGT202500.023.7135759
ACATGGG141900.023.6658063
Produced by FastQC (version 0.11.5)