FastQCFastQC Report
Thu 26 May 2016
SRR938611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900662
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56420.2968439417424034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47240.24854498064358627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31720.1668892207031024No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21350.11232928316554967No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20870.10980384729110174No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20560.10817283662218743No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19460.10238537940991087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33050.054.6831471
GTATCAA60250.041.6790921
TATCAAC76800.032.3861242
ATCAACG77750.031.868073
TCAACGC80050.030.9524364
CAACGCA80550.030.7603075
AACGCAG83250.029.9666966
TGGTATC14950.028.6298962
GTGGTAT15800.028.2951581
ACGCAGA92800.026.4766837
CGCAGAG93650.026.2363728
TATACCG950.001218236225.0302335
GTACATG64600.023.4118611
GCAGAGT101700.023.4113439
TACATGG63200.023.1767272
TAAGGTG11200.022.5048625
GAGTACT61550.022.08071912-13
ACATGGG65850.021.5218183
GTGTAGC11200.021.232121
CAGAGTA98900.020.78230310-11
Produced by FastQC (version 0.11.5)