##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938611_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1900662 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.106409240569864 31.0 31.0 34.0 28.0 34.0 2 31.1485587653144 31.0 31.0 34.0 27.0 34.0 3 31.184119006956525 31.0 31.0 34.0 27.0 34.0 4 34.83553835453121 37.0 35.0 37.0 32.0 37.0 5 34.815661595801885 37.0 35.0 37.0 32.0 37.0 6 34.83128194281782 37.0 35.0 37.0 32.0 37.0 7 34.74047621302473 37.0 35.0 37.0 31.0 37.0 8 34.70895719491419 37.0 35.0 37.0 31.0 37.0 9 36.19700872643321 39.0 35.0 39.0 32.0 39.0 10-11 36.02956101610913 39.0 35.0 39.0 30.0 39.0 12-13 35.89052472243881 38.0 35.0 39.0 30.0 39.0 14-15 36.84637668349238 39.0 36.0 41.0 30.5 41.0 16-17 36.66253573754828 39.0 36.0 41.0 30.0 41.0 18-19 36.816873805021615 39.0 36.0 41.0 30.0 41.0 20-21 36.79774047147783 39.0 36.0 41.0 30.0 41.0 22-23 36.709423348286016 39.0 36.0 41.0 30.0 41.0 24-25 36.56888941852891 39.0 36.0 41.0 30.0 41.0 26-27 36.34937748005695 39.0 35.5 41.0 29.0 41.0 28-29 36.13515396214582 39.0 35.0 40.5 28.5 41.0 30-31 35.86269178844003 38.5 35.0 40.0 27.0 41.0 32-33 35.569724390764904 38.0 34.5 40.0 27.0 41.0 34-35 35.30568875476018 38.0 34.0 40.0 26.0 41.0 36-37 35.01356343210944 38.0 34.0 40.0 25.0 41.0 38-39 34.85167588976893 38.0 34.0 40.0 25.0 41.0 40-41 34.72927879865016 38.0 33.5 40.0 24.0 41.0 42-43 34.42684785616801 38.0 33.0 40.0 24.0 41.0 44-45 34.064034004994056 38.0 33.0 40.0 23.0 41.0 46-47 33.655556327216516 38.0 33.0 40.0 20.0 41.0 48-49 33.269808887640195 38.0 32.0 40.0 18.5 41.0 50-51 32.56393482902273 36.5 31.0 39.5 15.5 40.5 52-53 32.57493441758714 37.0 31.0 39.0 15.0 40.0 54-55 32.66359563141684 37.0 31.0 40.0 13.5 41.0 56-57 32.342172095827664 37.0 31.0 40.0 10.0 41.0 58-59 31.956361520354488 36.0 31.0 39.0 8.0 41.0 60-61 31.468710375648065 36.0 30.0 39.0 6.5 40.5 62-63 30.886717627858083 35.0 29.0 39.0 2.0 40.0 64-65 30.35610697746364 35.0 28.5 38.0 2.0 40.0 66-67 29.809747077597173 34.5 28.0 38.0 2.0 40.0 68-69 29.205250854702204 34.0 27.0 37.5 2.0 39.5 70-71 28.553999080320438 34.0 26.0 37.0 2.0 39.0 72-73 27.903891381003042 33.5 25.5 36.0 2.0 39.0 74-75 27.228074744483763 33.0 24.5 35.5 2.0 38.5 76-77 26.473046496431245 32.0 22.5 35.0 2.0 37.5 78-79 25.686749143193268 31.5 20.0 35.0 2.0 37.0 80-81 24.975898923638184 31.0 17.5 35.0 2.0 36.0 82-83 24.23084825181963 31.0 10.5 34.0 2.0 36.0 84-85 23.521020307661225 30.0 6.0 34.0 2.0 35.0 86-87 22.750180726504766 29.5 2.0 34.0 2.0 35.0 88-89 21.927733863253962 29.0 2.0 34.0 2.0 35.0 90-91 21.014442599473234 29.0 2.0 34.0 2.0 35.0 92-93 20.062621076235544 26.5 2.0 33.5 2.0 35.0 94-95 19.05166673506389 25.5 2.0 33.0 2.0 35.0 96-97 17.81503628735672 24.0 2.0 32.5 2.0 34.0 98-99 16.306311169476743 10.0 2.0 32.0 2.0 34.0 100-101 12.845637467366632 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4048447406646929 1101 2 0.3669565705839233 1101 3 0.2809283015784274 1101 4 0.2022388375871884 1101 5 0.204956748138585 1101 6 0.4848884728047551 1101 7 0.3955823087690291 1101 8 0.4596430163189851 1101 9 0.6107926957969312 1101 10-11 0.536801983208413 1101 12-13 0.6548513468458026 1101 14-15 0.6416764176819285 1101 16-17 0.4839949652700213 1101 18-19 0.6454398996979336 1101 20-21 0.5347963891489442 1101 22-23 0.6705644472596006 1101 24-25 0.6270586714194835 1101 26-27 0.5404121160564941 1101 28-29 0.4578671512144794 1101 30-31 0.46381476205910843 1101 32-33 0.49407864879518115 1101 34-35 0.579737911986264 1101 36-37 0.5429051320374185 1101 38-39 0.34308139255035996 1101 40-41 0.4787276538501146 1101 42-43 0.6421544850622425 1101 44-45 0.7860552711657789 1101 46-47 0.6100908250388173 1101 48-49 0.5599265644646465 1101 50-51 0.7329081211434811 1101 52-53 0.6212848756529255 1101 54-55 0.5844152417124704 1101 56-57 0.6594100094509159 1101 58-59 0.7160379125106395 1101 60-61 0.7805984980398648 1101 62-63 0.6857939300540892 1101 64-65 0.7881895328204287 1101 66-67 0.5499180433563815 1101 68-69 0.6998182527395933 1101 70-71 0.6757530319100127 1101 72-73 0.8163644864667496 1101 74-75 0.6678790886250177 1101 76-77 0.7839053335495585 1101 78-79 0.9166734525991771 1101 80-81 0.9035373540388285 1101 82-83 1.0822898716153304 1101 84-85 1.029258232293916 1101 86-87 0.957534639024157 1101 88-89 0.9851220409216417 1101 90-91 0.9482518140770644 1101 92-93 0.9453806017900099 1101 94-95 0.7136314392359964 1101 96-97 0.5755016689328549 1101 98-99 0.8052559165257946 1101 100-101 0.7656338883044729 1102 1 0.5800833743756009 1102 2 0.45536493367858455 1102 3 0.3983325077241773 1102 4 0.4224479057752859 1102 5 0.46077722619842376 1102 6 0.35774096827439905 1102 7 0.3898111652210545 1102 8 0.6151187799942832 1102 9 0.7558327951941166 1102 10-11 0.7110407708124029 1102 12-13 0.7748555200100924 1102 14-15 0.9590316411588518 1102 16-17 1.013318328289735 1102 18-19 0.8405448147003014 1102 20-21 0.8228170824143319 1102 22-23 0.6988890349990058 1102 24-25 0.7980179760308062 1102 26-27 0.60806954210139 1102 28-29 0.6880934964251182 1102 30-31 0.7990994474334272 1102 32-33 0.8909998081149055 1102 34-35 0.39070779636451647 1102 36-37 0.8134583486016993 1102 38-39 0.6114274824881676 1102 40-41 0.8490146017280864 1102 42-43 0.8579110561897636 1102 44-45 0.8127543441052865 1102 46-47 1.0692771865015445 1102 48-49 1.1639641009148178 1102 50-51 1.1751983683912286 1102 52-53 0.869589833610739 1102 54-55 0.8508372092003285 1102 56-57 1.060586480759035 1102 58-59 1.1154209022157069 1102 60-61 1.177684411682904 1102 62-63 1.2134559820699522 1102 64-65 1.3463129305961061 1102 66-67 1.0053401433208045 1102 68-69 1.0246242877960086 1102 70-71 1.1992685373264038 1102 72-73 1.03162526487203 1102 74-75 1.106765942943337 1102 76-77 1.5173291670292919 1102 78-79 1.339065446732274 1102 80-81 1.4827252880869253 1102 82-83 1.565415479883093 1102 84-85 1.7428307760547739 1102 86-87 2.0791474997129775 1102 88-89 1.9118582256520824 1102 90-91 1.702418170968894 1102 92-93 1.638606522035456 1102 94-95 1.5583823848523828 1102 96-97 1.581373929108235 1102 98-99 1.7130042037386275 1102 100-101 1.625973774790733 1103 1 0.287349588461165 1103 2 0.2504351491039216 1103 3 0.24570892612478445 1103 4 0.16554277625049707 1103 5 0.149300945859558 1103 6 0.26412244349272385 1103 7 0.20971207120018676 1103 8 0.2661983574763269 1103 9 0.23339367715541215 1103 10-11 0.39895383276045493 1103 12-13 0.5794185014902169 1103 14-15 0.7310714830005551 1103 16-17 0.7011762472335974 1103 18-19 0.5884279360394231 1103 20-21 0.494861213156355 1103 22-23 0.5141589497928507 1103 24-25 0.5225643737629895 1103 26-27 0.45694196978235624 1103 28-29 0.6138505894493207 1103 30-31 0.6702333101396007 1103 32-33 0.6502791503201379 1103 34-35 0.6322341547205212 1103 36-37 0.6119933827903665 1103 38-39 0.7392959398728394 1103 40-41 0.7117085212928274 1103 42-43 0.748109080844813 1103 44-45 0.6831458185883719 1103 46-47 0.46518863909081887 1103 48-49 0.6487446317581416 1103 50-51 0.7560339278680672 1103 52-53 0.690978579536285 1103 54-55 0.6874270432811613 1103 56-57 0.6463236406083936 1103 58-59 0.609470563596819 1103 60-61 0.8081895216012924 1103 62-63 0.9410873245366034 1103 64-65 1.0566099675786838 1103 66-67 1.105051970065567 1103 68-69 1.0970393062995534 1103 70-71 1.1553185418195397 1103 72-73 1.0620437798505051 1103 74-75 1.3280691150957118 1103 76-77 1.294958875629149 1103 78-79 1.187480391711805 1103 80-81 1.167464563793775 1103 82-83 1.1580506210900268 1103 84-85 1.12783923131542 1103 86-87 1.17290388200513 1103 88-89 1.150167908763617 1103 90-91 1.1714308661138553 1103 92-93 1.284730377307536 1103 94-95 1.3049696455930153 1103 96-97 1.5109450799223048 1103 98-99 1.545558386846082 1103 100-101 1.3521234043114383 1104 1 0.32213544545292194 1104 2 0.1425686883816617 1104 3 0.2334591513764792 1104 4 0.14758140201929137 1104 5 0.2648620604217271 1104 6 0.29562718077369965 1104 7 0.25546502745378774 1104 8 0.44431340652803186 1104 9 0.3862953666830222 1104 10-11 0.5823990924015092 1104 12-13 0.4630669073797762 1104 14-15 0.6248471211204105 1104 16-17 0.6049448060145011 1104 18-19 0.5852793090954052 1104 20-21 0.6505140513277041 1104 22-23 0.6450759340504106 1104 24-25 0.5549457738491697 1104 26-27 0.7393867481402836 1104 28-29 0.5164847751014818 1104 30-31 0.7291629308773011 1104 32-33 0.7068745479103669 1104 34-35 0.563802969812663 1104 36-37 0.7118357066043686 1104 38-39 0.9083821734966193 1104 40-41 0.6000379651869707 1104 42-43 0.6583545352376348 1104 44-45 0.5611676889888955 1104 46-47 0.6393391184152293 1104 48-49 0.43735277215849777 1104 50-51 0.6058803090107219 1104 52-53 0.7467409655813171 1104 54-55 0.8834529598177099 1104 56-57 1.0700486600555834 1104 58-59 0.9725241091424301 1104 60-61 0.976581532301438 1104 62-63 1.0256402349004183 1104 64-65 1.1497340546797368 1104 66-67 1.080316116151785 1104 68-69 0.9522673841331049 1104 70-71 1.0155884800921804 1104 72-73 1.1341383128100055 1104 74-75 0.8301828434169884 1104 76-77 0.8567742363956192 1104 78-79 1.0460059584786485 1104 80-81 1.0159625127441885 1104 82-83 1.0677682030340776 1104 84-85 0.9654352780782283 1104 86-87 1.360969599536709 1104 88-89 1.1817107009892176 1104 90-91 1.072964956069029 1104 92-93 1.1228050489437322 1104 94-95 1.2965539065156229 1104 96-97 1.5890742596703618 1104 98-99 1.853327611588508 1104 100-101 1.7649370078348792 1105 1 0.4670408035061868 1105 2 0.4232389103593093 1105 3 0.37634812478717805 1105 4 0.4283781369597932 1105 5 0.3393118369334758 1105 6 0.39094593139749634 1105 7 0.30210595992716094 1105 8 0.3215664297562455 1105 9 0.5213130218502329 1105 10-11 0.599487648778009 1105 12-13 0.6698872778550538 1105 14-15 0.7096478969227178 1105 16-17 0.5672505519689182 1105 18-19 0.45987299747428523 1105 20-21 0.6309459487793703 1105 22-23 0.6857182908061716 1105 24-25 0.7832061801473102 1105 26-27 0.6963634390035693 1105 28-29 0.6567284406864502 1105 30-31 0.6783536795157445 1105 32-33 0.8423973108024114 1105 34-35 0.7141741464371734 1105 36-37 0.5734700436791371 1105 38-39 0.6907462934492514 1105 40-41 0.8820886351122255 1105 42-43 0.9084686396796116 1105 44-45 1.0212791498764275 1105 46-47 0.8810455058362265 1105 48-49 1.0000469587664682 1105 50-51 1.1481471029995944 1105 52-53 1.1104670825505067 1105 54-55 1.1741027347109565 1105 56-57 1.2373101264729982 1105 58-59 1.317096999761322 1105 60-61 1.183709148543656 1105 62-63 1.1789249861770088 1105 64-65 1.1733055237137329 1105 66-67 1.2941750852195035 1105 68-69 1.3506747774535448 1105 70-71 1.3312496519421018 1105 72-73 1.1426300570973673 1105 74-75 1.3221748965686153 1105 76-77 1.3838074130039395 1105 78-79 1.512105339940856 1105 80-81 1.4444199717488608 1105 82-83 1.3690287469049416 1105 84-85 1.4986074355153818 1105 86-87 1.604479943117859 1105 88-89 1.6180814244329582 1105 90-91 1.4417921889958478 1105 92-93 1.3466656904642598 1105 94-95 1.632649079870756 1105 96-97 1.8354396420551176 1105 98-99 1.8497549017024255 1105 100-101 1.75072520517352 1106 1 0.06402180337599717 1106 2 0.0799203917153477 1106 3 -0.08749119069585021 1106 4 -0.07827418426105481 1106 5 0.10635728546504453 1106 6 -0.15801451772151154 1106 7 0.09469589856915661 1106 8 0.07706531151004725 1106 9 -0.009631120878843547 1106 10-11 0.05496208047290452 1106 12-13 -0.06556864165239062 1106 14-15 0.12377738485804457 1106 16-17 0.10739310322142614 1106 18-19 -0.18085323278176446 1106 20-21 0.14679720774571337 1106 22-23 0.13688511959120575 1106 24-25 -0.09504787395823655 1106 26-27 -0.0710458496902362 1106 28-29 0.03659635744617873 1106 30-31 -0.3536893928088105 1106 32-33 -0.06043963694114751 1106 34-35 -0.49586614853003397 1106 36-37 -0.22400620218896705 1106 38-39 -0.19328845315394005 1106 40-41 0.19748857351077476 1106 42-43 -0.3636906815613443 1106 44-45 -0.35053453644096066 1106 46-47 -0.1481776067726983 1106 48-49 0.03054443808182583 1106 50-51 -0.22156319026350957 1106 52-53 -0.19159884158234064 1106 54-55 -0.10445408733178141 1106 56-57 -0.9503959393643342 1106 58-59 -0.7337638857541897 1106 60-61 -0.109404719931959 1106 62-63 -0.08503509777877838 1106 64-65 0.04416264116821722 1106 66-67 -0.3371341678047379 1106 68-69 -0.19963624634769062 1106 70-71 -0.28222825780013494 1106 72-73 -0.10722811139211785 1106 74-75 0.19643729333062154 1106 76-77 0.15366382447620097 1106 78-79 0.37867893408893494 1106 80-81 0.4165527260427062 1106 82-83 0.15146820654805637 1106 84-85 0.18667390157160924 1106 86-87 0.3411538671829639 1106 88-89 0.49136211428448817 1106 90-91 0.5347317846490292 1106 92-93 0.5641015990273921 1106 94-95 0.44361784200978605 1106 96-97 0.317262452160719 1106 98-99 0.27470936214466235 1106 100-101 0.005432370734290259 1107 1 0.24653857293453996 1107 2 0.02046099649944466 1107 3 -0.0186004851729642 1107 4 0.1942065322322506 1107 5 0.11298139673399277 1107 6 0.2355038883578544 1107 7 0.1623851797453426 1107 8 0.2873962173633302 1107 9 0.372215641450488 1107 10-11 0.37834197911919887 1107 12-13 0.4288566201935211 1107 14-15 0.421482457659188 1107 16-17 0.5478111110479986 1107 18-19 0.3932831756467081 1107 20-21 0.5208345826360983 1107 22-23 0.5697748474388717 1107 24-25 0.5008128703667794 1107 26-27 0.5733096778694033 1107 28-29 0.4205813967417882 1107 30-31 0.44242786207350093 1107 32-33 0.5017725702536566 1107 34-35 0.5266701176911326 1107 36-37 0.4876505335643415 1107 38-39 0.5721295675911691 1107 40-41 0.5414195461270879 1107 42-43 0.5857227649866417 1107 44-45 0.554573480650788 1107 46-47 0.5940799238326164 1107 48-49 0.6408363557772745 1107 50-51 0.69605695087931 1107 52-53 0.694795376491939 1107 54-55 0.785680177652317 1107 56-57 0.7668740317963127 1107 58-59 0.8422194865328443 1107 60-61 0.8221531063012577 1107 62-63 1.0233420337159558 1107 64-65 0.9668814722159134 1107 66-67 1.0215484871932112 1107 68-69 1.1281570135842145 1107 70-71 1.3449956045026852 1107 72-73 1.2619294136840615 1107 74-75 1.2274777061451978 1107 76-77 1.3026014294716468 1107 78-79 1.3997573429173578 1107 80-81 1.3475252131667936 1107 82-83 1.3061362181210043 1107 84-85 1.3909081601997748 1107 86-87 1.4097765129725772 1107 88-89 1.3364304352823417 1107 90-91 1.4014674707760157 1107 92-93 1.6004608336916597 1107 94-95 1.6055087392438203 1107 96-97 1.6991005590673325 1107 98-99 1.508586634968239 1107 100-101 1.1327947198005113 1108 1 0.40996414658058455 1108 2 0.2697330419477879 1108 3 0.3444842412117808 1108 4 0.3065295050366643 1108 5 0.15468455147156135 1108 6 0.28122364313760784 1108 7 0.4393196257001861 1108 8 0.42276681159616913 1108 9 0.4123862334385606 1108 10-11 0.4801233537371701 1108 12-13 0.4244454005485565 1108 14-15 0.5071785556697392 1108 16-17 0.4980978416003481 1108 18-19 0.6335635315747083 1108 20-21 0.5486574328712024 1108 22-23 0.40572520342554697 1108 24-25 0.5981473131585417 1108 26-27 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64-65 -1.7395479568749899 2316 66-67 -1.6872555034750576 2316 68-69 -2.0248405434552446 2316 70-71 -2.2098311398121595 2316 72-73 -2.1433118627462413 2316 74-75 -2.2770425199324187 2316 76-77 -2.507704047431236 2316 78-79 -2.58284500563542 2316 80-81 -2.766365081769056 2316 82-83 -2.7399104076241727 2316 84-85 -2.6305937975824705 2316 86-87 -2.658272598712742 2316 88-89 -2.798692519533329 2316 90-91 -2.837075088484873 2316 92-93 -2.8242559737147808 2316 94-95 -3.3765811696615273 2316 96-97 -3.198076024958157 2316 98-99 -2.978667948437854 2316 100-101 -2.5733334836840775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7457.0 3 2593.0 4 2710.0 5 3255.0 6 3664.0 7 4576.0 8 5946.0 9 7497.0 10 9328.0 11 11446.0 12 13783.0 13 15919.0 14 18277.0 15 21063.0 16 23960.0 17 27048.0 18 26151.0 19 24923.0 20 26299.0 21 28379.0 22 31442.0 23 34375.0 24 38857.0 25 42460.0 26 47547.0 27 53079.0 28 60083.0 29 67824.0 30 78290.0 31 90991.0 32 106303.0 33 125315.0 34 151445.0 35 183559.0 36 211678.0 37 200515.0 38 88489.0 39 4136.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.326744009231014 18.44609110314967 14.84797287520606 23.379192012413256 2 15.583257532704001 19.527593974784857 40.53362940787024 24.355519084640907 3 19.372598968712122 23.447234333279077 29.453177223171267 27.72698947483753 4 13.001666691249133 16.352748565613062 36.716776269350795 33.928808473787015 5 13.971138062503458 36.997135938829764 34.06699321004081 14.964732788625962 6 32.70365991420965 35.79100649615681 17.144429552115398 14.360904037518136 7 29.112448457067075 32.32009641521697 20.171989590103756 18.395465537612196 8 26.55767555804658 35.04014146214388 19.36278882512216 19.03939415468738 9 26.2890691399645 16.918474951702173 19.903230678632493 36.889225229700834 10-11 25.157732131609833 26.403165197149704 27.0730480525336 21.36605461870687 12-13 26.15101032221656 23.689613348550637 27.403226587604074 22.75614974162873 14-15 22.751384986699446 25.22358591627794 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40-41 22.825967387339684 26.22331687840176 28.537051491707356 22.4136642425512 42-43 22.44373907583613 26.257650533070247 28.35722154006961 22.94138885102401 44-45 22.438813794358996 26.60817598033812 28.49033467234543 22.46267555295745 46-47 22.904366215600067 26.802711924696705 27.709273968958993 22.58364789074424 48-49 22.625617339491733 26.842941887740768 27.722239586128662 22.80920118663884 50-51 22.78598902399882 27.09780532593883 27.612456656371187 22.50374899369117 52-53 23.067336427464753 26.763114256165355 27.655836329726263 22.513712986643633 54-55 22.52560903258566 26.95589603002443 27.82126888201626 22.697226055373655 56-57 22.619700055593984 27.039171789092265 27.620053931162914 22.721074224150836 58-59 22.601636070378916 26.669104897423278 27.64727396153735 23.081985070660455 60-61 22.38275469370657 26.912242054011042 27.93371132783638 22.771291924446007 62-63 22.442549344710248 26.924760728603697 28.02670620803312 22.605983718652933 64-65 22.11160472572117 26.91477948090311 27.868268917591383 23.105346875784335 66-67 22.14215769586752 26.959175071994935 27.757709758740024 23.140957473397524 68-69 22.575067504122075 26.574431861682523 27.93774808406161 22.912752550133796 70-71 22.343920101491506 26.722833299255473 27.654566788794355 23.278679810458666 72-73 22.402023873774187 26.768672585420326 27.624592887233085 23.2047106535724 74-75 22.389371495575492 27.081050556339726 27.51613337838364 23.013444569701143 76-77 22.445514904446664 26.71825363516832 27.601913776893905 23.23431768349111 78-79 22.610893151273075 26.905346500896176 27.536969645310812 22.94679070251994 80-81 22.704929051864998 26.998753274332 27.212556597899006 23.083761075903993 82-83 22.625181130252777 26.978200178262686 27.38605236545324 23.0105663260313 84-85 22.609845191383123 26.972925263308817 27.3364999230495 23.080729622258556 86-87 22.721781121130526 26.92850689399875 27.13490852816297 23.21480345670775 88-89 22.593707539788745 26.954501996246726 27.082269155270914 23.369521308693614 90-91 22.534069281750277 26.98047108558532 27.112974433038307 23.372485199626094 92-93 22.670830008904908 26.551464257219187 27.299776429995433 23.477929303880476 94-95 22.41878519084681 26.428353916516052 27.439365741614402 23.713495151022737 96-97 22.66144931518314 26.081546382554716 27.4688923576486 23.78811194461354 98-99 22.41432799249119 26.301272755547732 27.111148300795428 24.17325095116565 100-101 23.04144675043492 25.62547124906803 27.446440832178464 23.886641168318583 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 306.0 1 214.0 2 182.0 3 273.0 4 264.0 5 245.5 6 391.5 7 839.0 8 1403.0 9 1677.5 10 1544.5 11 1345.0 12 1359.0 13 1450.0 14 1591.0 15 1770.0 16 1972.0 17 2199.0 18 2404.5 19 2695.5 20 3104.5 21 3571.0 22 4235.0 23 5204.5 24 6512.0 25 8221.5 26 10374.5 27 13080.5 28 15549.5 29 17983.5 30 21024.5 31 25329.0 32 30893.0 33 33842.5 34 34704.0 35 37309.0 36 41404.5 37 45510.0 38 48608.0 39 50720.0 40 53630.0 41 57584.0 42 60120.5 43 62159.0 44 64696.5 45 67963.5 46 69493.0 47 70411.5 48 71268.5 49 70194.0 50 69575.5 51 68264.0 52 66273.0 53 66024.5 54 65900.5 55 62058.5 56 56878.5 57 52305.5 58 48156.5 59 42574.5 60 35221.0 61 29797.5 62 25375.0 63 19593.0 64 14530.0 65 11509.5 66 9014.0 67 7029.5 68 5607.5 69 4287.0 70 3176.0 71 2235.0 72 1620.0 73 1354.5 74 1069.0 75 802.5 76 577.5 77 385.5 78 265.0 79 183.0 80 117.0 81 76.5 82 51.5 83 28.5 84 20.0 85 13.5 86 8.5 87 6.0 88 4.0 89 3.5 90 4.0 91 3.5 92 1.5 93 1.5 94 2.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0059452969544295615 2 0.10738363791142246 3 0.11885332584120689 4 0.12048433651012119 5 0.15820803488468754 6 0.09717666791886195 7 0.028779446319229827 8 0.02667491642385653 9 0.02488606601278923 10-11 0.05269216725540891 12-13 0.05629617470123568 14-15 0.029121432427227985 16-17 0.03348833196012757 18-19 0.0393020957960963 20-21 0.060478927868290094 22-23 0.033698784949664905 24-25 0.05071917047849644 26-27 0.05916359668368179 28-29 0.05603310846431401 30-31 0.07023868525808376 32-33 0.0525343275132559 34-35 0.06531934662764868 36-37 0.055033456764011696 38-39 0.03259390675459392 40-41 0.038144604353641 42-43 0.033198959099513745 44-45 0.006918642031039711 46-47 0.012653485995931944 48-49 0.008839025560567844 50-51 0.007997213602418526 52-53 0.009601917647640663 54-55 0.011022475327017639 56-57 0.014547562901767912 58-59 0.019835194263893317 60-61 0.02409686730202424 62-63 0.010785715713788144 64-65 0.008891638807952176 66-67 0.009522997776564165 68-69 0.01170644754301396 70-71 0.03164686830167594 72-73 0.02859529995338466 74-75 0.029673871524763477 76-77 0.023176135472798426 78-79 0.004340592909207424 80-81 0.004393206156591755 82-83 0.005050871748895912 84-85 0.00470888564089775 86-87 0.01267979261962411 88-89 0.037960457987795834 90-91 0.03735540564287601 92-93 0.030831362967218792 94-95 0.049009239938505644 96-97 0.044589727158221716 98-99 0.025964637584168043 100-101 0.07649966169681932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1900662.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.04974226097676 #Duplication Level Percentage of deduplicated Percentage of total 1 81.34434041801424 50.473953573269384 2 11.050333827300458 13.713407317634926 3 3.329213597924178 6.1973053704880305 4 1.385640489495807 3.4391454095835403 5 0.7664175766236443 2.377800654688977 6 0.45695034435058984 1.7012190655811208 7 0.3112179198142064 1.3517694198028185 8 0.2192565685670223 1.088385085488793 9 0.16396705334660708 0.9156702059503916 >10 0.8861996451753494 9.816836008360546 >50 0.053646820696749566 2.264017064328707 >100 0.028226120522037896 3.28162673117323 >500 0.0028047666589876637 1.258800778088021 >1k 0.0016998585812046437 1.8225161208750185 >5k 8.499292906023218E-5 0.29754719468652274 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5642 0.2968439417424034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4724 0.24854498064358627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3172 0.1668892207031024 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2135 0.11232928316554967 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2087 0.10980384729110174 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2056 0.10817283662218743 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1946 0.10238537940991087 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.6306623692166203E-5 1.0522649476866481E-4 14-15 0.0 0.0 0.0 5.2613247384332406E-5 1.578397421529972E-4 16-17 0.0 0.0 0.0 1.0522649476866481E-4 2.1045298953732962E-4 18-19 0.0 0.0 0.0 1.3153311846083102E-4 2.1045298953732962E-4 20-21 0.0 0.0 0.0 1.578397421529972E-4 2.1045298953732962E-4 22-23 0.0 0.0 0.0 4.7351922645899163E-4 2.1045298953732962E-4 24-25 0.0 0.0 0.0 8.944252055336509E-4 2.1045298953732962E-4 26-27 0.0 0.0 0.0 0.0016047040452221383 2.1045298953732962E-4 28-29 0.0 0.0 0.0 0.003367247832597274 2.1045298953732962E-4 30-31 0.0 0.0 0.0 0.00983867726087016 2.1045298953732962E-4 32-33 0.0 0.0 0.0 0.025122825626018724 2.1045298953732962E-4 34-35 0.0 0.0 0.0 0.04722038952743833 2.6306623692166204E-4 36-37 0.0 0.0 0.0 0.08544391375215582 2.6306623692166204E-4 38-39 0.0 5.2613247384332406E-5 0.0 0.14223991430354266 3.156794843059944E-4 40-41 0.0 5.2613247384332406E-5 0.0 0.2037711071195194 3.156794843059944E-4 42-43 0.0 5.2613247384332406E-5 0.0 0.26664393774379663 3.156794843059944E-4 44-45 0.0 5.2613247384332406E-5 5.2613247384332406E-5 0.34943088250304366 3.156794843059944E-4 46-47 0.0 5.2613247384332406E-5 5.2613247384332406E-5 0.45031678436250105 3.156794843059944E-4 48-49 0.0 5.2613247384332406E-5 5.2613247384332406E-5 0.554938226786246 3.156794843059944E-4 50-51 0.0 5.2613247384332406E-5 5.2613247384332406E-5 0.6701349319342418 4.2090597907465925E-4 52-53 0.0 5.2613247384332406E-5 5.2613247384332406E-5 0.7837532396607076 5.261324738433241E-4 54-55 0.0 5.2613247384332406E-5 5.2613247384332406E-5 0.9057370537212823 5.261324738433241E-4 56-57 0.0 5.2613247384332406E-5 5.2613247384332406E-5 1.0508706966309633 5.261324738433241E-4 58-59 0.0 5.2613247384332406E-5 5.2613247384332406E-5 1.2094996374947256 5.261324738433241E-4 60-61 0.0 5.2613247384332406E-5 5.2613247384332406E-5 1.3873850269011534 5.261324738433241E-4 62-63 0.0 1.0522649476866481E-4 5.2613247384332406E-5 1.5590357464925377 5.261324738433241E-4 64-65 0.0 1.0522649476866481E-4 5.2613247384332406E-5 1.742813819606011 5.261324738433241E-4 66-67 0.0 1.0522649476866481E-4 5.2613247384332406E-5 1.9545558337042568 5.524390975354903E-4 68-69 0.0 1.0522649476866481E-4 5.2613247384332406E-5 2.190815621083601 5.787457212276565E-4 70-71 0.0 1.578397421529972E-4 5.2613247384332406E-5 2.438624016263807 5.787457212276565E-4 72-73 0.0 1.578397421529972E-4 5.2613247384332406E-5 2.7012693472063942 6.313589686119888E-4 74-75 0.0 1.578397421529972E-4 5.2613247384332406E-5 2.9607841899296137 7.628920870728199E-4 76-77 0.0 1.578397421529972E-4 5.2613247384332406E-5 3.248999559100987 8.418119581493185E-4 78-79 0.0 1.578397421529972E-4 5.2613247384332406E-5 3.5755699856155383 8.418119581493185E-4 80-81 0.0 1.578397421529972E-4 5.2613247384332406E-5 3.919581703637996 8.418119581493185E-4 82-83 0.0 1.578397421529972E-4 5.2613247384332406E-5 4.273616245287169 9.733450766101495E-4 84-85 0.0 1.578397421529972E-4 5.2613247384332406E-5 4.628255839281261 9.996517003023158E-4 86-87 0.0 1.578397421529972E-4 5.2613247384332406E-5 4.994786027184213 0.0010259583239944818 88-89 0.0 1.578397421529972E-4 5.2613247384332406E-5 5.387964824887328 0.0010522649476866481 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3305 0.0 54.683147 1 GTATCAA 6025 0.0 41.679092 1 TATCAAC 7680 0.0 32.386124 2 ATCAACG 7775 0.0 31.86807 3 TCAACGC 8005 0.0 30.952436 4 CAACGCA 8055 0.0 30.760307 5 AACGCAG 8325 0.0 29.966696 6 TGGTATC 1495 0.0 28.629896 2 GTGGTAT 1580 0.0 28.295158 1 ACGCAGA 9280 0.0 26.476683 7 CGCAGAG 9365 0.0 26.236372 8 TATACCG 95 0.0012182362 25.030233 5 GTACATG 6460 0.0 23.411861 1 GCAGAGT 10170 0.0 23.411343 9 TACATGG 6320 0.0 23.176727 2 TAAGGTG 1120 0.0 22.504862 5 GAGTACT 6155 0.0 22.080719 12-13 ACATGGG 6585 0.0 21.521818 3 GTGTAGC 1120 0.0 21.23212 1 CAGAGTA 9890 0.0 20.782303 10-11 >>END_MODULE