FastQCFastQC Report
Thu 26 May 2016
SRR938611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900662
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56750.2985801789060864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.25885717713091544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.16973033606185634No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20920.11006691352802339No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20680.10880419559079942No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20170.10612091997419847No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20030.10538433451081781No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19790.10412161657359384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33250.051.0281451
GTATCAA59500.041.8550151
ATCAACG76650.032.410273
TATCAAC77500.032.379152
TCAACGC79000.031.5062944
CAACGCA79900.031.0919555
AACGCAG83200.029.8469586
GTGGTAT15900.028.695071
TGGTATC16800.026.026262
ACGCAGA95950.025.68297
CGCAGAG96850.025.3461868
TAAGGTG11750.025.0638035
GTACATG61200.023.9960781
TACATGG61200.023.5301362
GTAAGGT11250.023.2221974
GCAGAGT104800.022.6985539
ACATGGG62050.022.5060183
GGTAAGG12000.020.9791433
AGAGTAC95400.020.8290210-11
AGGTAAG11000.020.7387122
Produced by FastQC (version 0.11.5)