##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938611_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1900662 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80377573708529 31.0 31.0 34.0 30.0 34.0 2 32.027376777143964 31.0 31.0 34.0 30.0 34.0 3 32.08595426225178 33.0 31.0 34.0 30.0 34.0 4 35.759503267808796 37.0 35.0 37.0 35.0 37.0 5 35.65686587094391 37.0 35.0 37.0 33.0 37.0 6 35.65686060961917 37.0 35.0 37.0 33.0 37.0 7 35.60112318760516 37.0 35.0 37.0 33.0 37.0 8 35.57327289123474 37.0 35.0 37.0 33.0 37.0 9 37.160988118876475 39.0 37.0 39.0 33.0 39.0 10-11 37.058876328352966 39.0 37.0 39.0 33.0 39.0 12-13 36.948433756238614 39.0 37.0 39.0 33.0 39.0 14-15 37.990925530157384 40.0 37.0 41.0 33.0 41.0 16-17 37.90161296432506 40.0 37.0 41.0 33.0 41.0 18-19 38.13860065598197 40.0 37.0 41.0 33.0 41.0 20-21 38.11256446438136 40.0 37.0 41.0 33.0 41.0 22-23 38.007842793721345 40.0 37.0 41.0 33.0 41.0 24-25 37.88606417132557 40.0 37.0 41.0 32.5 41.0 26-27 37.69331632873178 40.0 37.0 41.0 32.0 41.0 28-29 37.57634182195467 39.5 37.0 41.0 32.0 41.0 30-31 37.31336081849376 39.0 36.0 41.0 31.0 41.0 32-33 37.101849250419065 39.0 36.0 41.0 31.0 41.0 34-35 37.001220627339315 39.0 36.0 41.0 31.0 41.0 36-37 36.952075645222564 39.0 36.0 41.0 31.0 41.0 38-39 36.783810325034125 39.0 35.0 41.0 30.0 41.0 40-41 36.599021814504624 39.0 35.0 41.0 30.0 41.0 42-43 36.37839131839328 39.0 35.0 40.0 30.0 41.0 44-45 36.078149087002316 39.0 35.0 40.0 29.5 41.0 46-47 35.69531300147001 38.0 35.0 40.0 28.5 41.0 48-49 35.32982560813022 38.0 34.0 40.0 27.0 41.0 50-51 34.951867822895395 38.0 34.0 40.0 26.0 41.0 52-53 34.451875451816264 38.0 33.0 40.0 24.0 41.0 54-55 34.1069958782782 37.5 33.0 40.0 24.0 41.0 56-57 33.76891156870606 37.0 33.0 40.0 23.0 41.0 58-59 33.28662539683542 36.5 32.0 39.0 22.0 41.0 60-61 32.87783493330218 36.0 31.0 39.0 21.5 40.0 62-63 32.388348375460765 35.5 31.0 39.0 19.5 40.0 64-65 31.857763242491302 35.0 30.5 38.5 18.0 40.0 66-67 31.351831098848717 35.0 30.0 38.0 14.5 40.0 68-69 30.85059521366766 34.0 29.0 37.5 12.0 39.5 70-71 30.264939531594784 34.0 29.0 37.0 8.0 39.0 72-73 29.752746674579697 34.0 28.0 36.0 7.0 39.0 74-75 29.158915946128243 34.0 27.5 36.0 4.5 38.5 76-77 27.95883092312047 31.5 26.0 34.5 2.0 36.5 78-79 28.63817422561192 33.0 27.5 35.0 2.0 37.0 80-81 28.252877681565685 33.0 26.5 35.0 2.0 37.0 82-83 27.795930838834046 33.0 26.0 35.0 2.0 36.0 84-85 27.227976357711157 32.5 26.0 35.0 2.0 36.0 86-87 26.612390314532515 32.0 25.0 34.5 2.0 35.0 88-89 25.85686171449737 31.5 23.5 34.0 2.0 35.0 90-91 25.234708748846455 31.0 20.5 34.0 2.0 35.0 92-93 24.40119863500191 31.0 17.5 34.0 2.0 35.0 94-95 23.574864178901876 30.5 5.0 34.0 2.0 35.0 96-97 22.50555306519518 30.0 2.0 34.0 2.0 35.0 98-99 21.147918462093735 29.0 2.0 34.0 2.0 35.0 100-101 17.249282618371915 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1661399729459525 1101 2 0.24818660081427169 1101 3 0.20331208763592912 1101 4 0.10489701399799145 1101 5 0.039550175533833 1101 6 0.2133018529120676 1101 7 0.21583770825138515 1101 8 0.13562235648443988 1101 9 0.18026097683880948 1101 10-11 0.14109170452866948 1101 12-13 0.20940380184438823 1101 14-15 0.3417127961609694 1101 16-17 0.4008698135024886 1101 18-19 0.41797938154650893 1101 20-21 0.22884998644234855 1101 22-23 0.3882792818215677 1101 24-25 0.4140072280445679 1101 26-27 0.29873704872697004 1101 28-29 0.2935948568732698 1101 30-31 0.34916744797784105 1101 32-33 0.3630713350749275 1101 34-35 0.3020805162924205 1101 36-37 0.36795018257821965 1101 38-39 0.3730682161711272 1101 40-41 0.41602332385409824 1101 42-43 0.32461141343142685 1101 44-45 0.3689309402308467 1101 46-47 0.40104693831064253 1101 48-49 0.5123190138501101 1101 50-51 0.6708714208754216 1101 52-53 0.6711932889811294 1101 54-55 0.6020176426118269 1101 56-57 0.38551467130615436 1101 58-59 0.4655882545163834 1101 60-61 0.4731113632344588 1101 62-63 0.5124830519619934 1101 64-65 0.2601401489764186 1101 66-67 0.40415258784098995 1101 68-69 0.46471454537366697 1101 70-71 0.3149725440248119 1101 72-73 0.5171288077633136 1101 74-75 0.517395331290448 1101 76-77 0.3874865567774499 1101 78-79 0.47177208501912204 1101 80-81 0.7078730353676335 1101 82-83 0.5703841076010434 1101 84-85 0.6976593346815427 1101 86-87 0.6520993438999056 1101 88-89 0.7246025692178684 1101 90-91 0.7718900635412673 1101 92-93 0.8064983768458092 1101 94-95 0.8597418301894635 1101 96-97 0.6889651639668024 1101 98-99 0.8135666856981558 1101 100-101 1.1533674717234668 1102 1 0.3431176290778275 1102 2 0.35081768299470895 1102 3 0.34166105044856465 1102 4 0.25924382047178085 1102 5 0.10763180189305643 1102 6 0.2485608078525985 1102 7 0.11594702435114357 1102 8 0.24552404542276918 1102 9 0.31247471505474067 1102 10-11 0.40915295458718504 1102 12-13 0.4021974140882776 1102 14-15 0.5285787711960239 1102 16-17 0.5330061462517577 1102 18-19 0.4528543686583433 1102 20-21 0.45500902622294603 1102 22-23 0.49914838611793755 1102 24-25 0.45785773085885495 1102 26-27 0.3891943620597189 1102 28-29 0.46218472813848877 1102 30-31 0.42751983131663707 1102 32-33 0.4014427240563734 1102 34-35 0.5539348055496163 1102 36-37 0.6422174847651902 1102 38-39 0.573059618963164 1102 40-41 0.38061001690592633 1102 42-43 0.3927288359945962 1102 44-45 0.4770597992222534 1102 46-47 0.5346328643446654 1102 48-49 0.6078557167382499 1102 50-51 0.6471371608793319 1102 52-53 0.639429053182667 1102 54-55 0.680546712341922 1102 56-57 0.7398749090051808 1102 58-59 0.9921906471101707 1102 60-61 0.9080661422265521 1102 62-63 0.902050233023175 1102 64-65 1.035290883119849 1102 66-67 0.9481130798460455 1102 68-69 1.0875473835261786 1102 70-71 1.199526573805418 1102 72-73 1.0854287297765417 1102 74-75 1.1989634681408639 1102 76-77 1.1106472820391353 1102 78-79 1.1447726911069829 1102 80-81 1.1222532577507849 1102 82-83 1.1118382534286582 1102 84-85 1.1887479156006968 1102 86-87 1.034015621780025 1102 88-89 1.104183527228308 1102 90-91 1.2828839344356773 1102 92-93 1.7252656228908627 1102 94-95 1.8308523049236136 1102 96-97 1.6440208882540333 1102 98-99 1.6500606402700164 1102 100-101 1.5607229185442257 1103 1 0.23013982515817943 1103 2 0.26469860678299284 1103 3 0.23863962228560354 1103 4 0.14403783071612253 1103 5 0.03105655007333752 1103 6 -0.003054677936844996 1103 7 0.2333648612749002 1103 8 0.1845052477853386 1103 9 0.27685846134790637 1103 10-11 0.23363277224765255 1103 12-13 0.28997629472614506 1103 14-15 0.45400602736182805 1103 16-17 0.30117474496591257 1103 18-19 0.27286862414609203 1103 20-21 0.3312129818144385 1103 22-23 0.3107982648033243 1103 24-25 0.32122153580783674 1103 26-27 0.29612930965343764 1103 28-29 0.284711053817162 1103 30-31 0.3090910393160513 1103 32-33 0.37774552858871147 1103 34-35 0.36561838273756564 1103 36-37 0.4176782352002064 1103 38-39 0.1924758792054675 1103 40-41 0.41749047504217884 1103 42-43 0.45356995061852245 1103 44-45 0.5072077050134922 1103 46-47 0.458719328575242 1103 48-49 0.5509154590774514 1103 50-51 0.649324500045573 1103 52-53 0.7204106395039318 1103 54-55 0.6712244811334287 1103 56-57 0.7201531156729999 1103 58-59 0.7303512398770451 1103 60-61 0.9382896143568686 1103 62-63 0.9639308953927639 1103 64-65 0.787521111289351 1103 66-67 0.8212841702070506 1103 68-69 0.6883518586873159 1103 70-71 0.7231124620979443 1103 72-73 0.7969613785196863 1103 74-75 0.912301539687892 1103 76-77 0.768583994208992 1103 78-79 0.7821226817122486 1103 80-81 0.9074763622610753 1103 82-83 0.9855464774971523 1103 84-85 1.1154230375280072 1103 86-87 1.0058855414754717 1103 88-89 0.9474803844855337 1103 90-91 1.1690391599501417 1103 92-93 1.4287719010240068 1103 94-95 1.4603413539314474 1103 96-97 1.3908736523481764 1103 98-99 1.6727979389980518 1103 100-101 1.662194215589377 1104 1 0.22760189613065407 1104 2 0.2088000903662035 1104 3 0.1989547508049867 1104 4 0.1160453409713682 1104 5 0.03049747816628212 1104 6 0.010407818355595566 1104 7 0.1895076568582823 1104 8 0.22062974341741892 1104 9 0.2951984263149754 1104 10-11 0.3367921720987894 1104 12-13 0.34188253965447757 1104 14-15 0.43990149378673493 1104 16-17 0.34293249166548634 1104 18-19 0.30277183587148926 1104 20-21 0.25686956951710016 1104 22-23 0.3769201301671643 1104 24-25 0.32017278630451074 1104 26-27 0.13722025537477833 1104 28-29 0.20109020978087955 1104 30-31 0.26837420727826355 1104 32-33 0.3370197424463086 1104 34-35 0.40746776910157934 1104 36-37 0.42545837275763887 1104 38-39 0.4826631598998574 1104 40-41 0.5841789673388504 1104 42-43 0.5602553089785829 1104 44-45 0.40919845430416046 1104 46-47 0.43827766736497864 1104 48-49 0.4639599106105621 1104 50-51 0.5465165488006747 1104 52-53 0.4373922388189442 1104 54-55 0.4067119761666973 1104 56-57 0.4158955788352472 1104 58-59 0.4864598979365766 1104 60-61 0.5506612488727072 1104 62-63 0.7258101940625252 1104 64-65 0.5700205936301757 1104 66-67 0.4248271974778959 1104 68-69 0.47891828974910666 1104 70-71 0.518153986758108 1104 72-73 0.44037703365235004 1104 74-75 0.5524567814766961 1104 76-77 0.5672337432142456 1104 78-79 0.8444529034942292 1104 80-81 0.5594606757890475 1104 82-83 0.8168195273211474 1104 84-85 1.0175016489702315 1104 86-87 0.9330812558171466 1104 88-89 1.1203112526419403 1104 90-91 0.9594153162888084 1104 92-93 1.1397513121797012 1104 94-95 1.0102928058954745 1104 96-97 1.0420922500234688 1104 98-99 1.2368687547729458 1104 100-101 1.3966283441591294 1105 1 0.16230070626140147 1105 2 0.24081110697473918 1105 3 0.1851084600086068 1105 4 0.16277124483770677 1105 5 0.10740144430720022 1105 6 0.14662856054699347 1105 7 0.07547496849828406 1105 8 0.138490460468077 1105 9 0.3105019090630847 1105 10-11 0.26128996419132733 1105 12-13 0.3669635322282474 1105 14-15 0.46245236197857054 1105 16-17 0.4489827245949982 1105 18-19 0.3458138926647507 1105 20-21 0.2938650350119687 1105 22-23 0.10472303969556407 1105 24-25 0.26753314323831034 1105 26-27 0.16440867336928733 1105 28-29 0.2561584161831334 1105 30-31 0.46000911082898455 1105 32-33 0.35565275504635707 1105 34-35 0.41483721662567774 1105 36-37 0.3834077837575194 1105 38-39 0.26777576722681573 1105 40-41 0.4137322875623184 1105 42-43 0.4092348430116459 1105 44-45 0.5209576858390719 1105 46-47 0.6906646891311468 1105 48-49 0.6571187693503404 1105 50-51 0.5949611676837421 1105 52-53 0.3971125615483828 1105 54-55 0.6491412948040249 1105 56-57 0.8255108337629196 1105 58-59 0.8078504345511561 1105 60-61 0.9187758011004377 1105 62-63 0.9779934193608 1105 64-65 0.8958566177242773 1105 66-67 0.7195983546803646 1105 68-69 1.0371404184578417 1105 70-71 1.06079519437694 1105 72-73 1.0175017905397503 1105 74-75 1.0078592086912828 1105 76-77 0.9381261856157792 1105 78-79 1.0959447583792752 1105 80-81 1.087334394811581 1105 82-83 1.1282310505210305 1105 84-85 1.1117051163425664 1105 86-87 1.1822960122459776 1105 88-89 1.3189407854611304 1105 90-91 1.4238883722647238 1105 92-93 1.4767501495552473 1105 94-95 1.7033867896511055 1105 96-97 1.7996609082577386 1105 98-99 1.9552755816174319 1105 100-101 1.7039561152381033 1106 1 -0.06898467884819581 1106 2 0.10748207798077658 1106 3 0.049737692329678396 1106 4 -0.07836878414249782 1106 5 -0.08720567711645089 1106 6 -0.13026729807874204 1106 7 -0.011675202493357517 1106 8 0.03646967897645226 1106 9 -0.23793461022229678 1106 10-11 0.0462278811521486 1106 12-13 -0.017027288944447605 1106 14-15 0.02800789166104778 1106 16-17 0.01690863985808022 1106 18-19 0.130289534161264 1106 20-21 0.10522489224902642 1106 22-23 0.1211963991865801 1106 24-25 -0.0017188966480929935 1106 26-27 0.05804139114680851 1106 28-29 0.1399767109661667 1106 30-31 0.12538729820111172 1106 32-33 0.09038708028294451 1106 34-35 0.10111544136087502 1106 36-37 0.01830674997516013 1106 38-39 -0.07165376315211347 1106 40-41 0.057716408783797135 1106 42-43 0.12743177288866292 1106 44-45 0.011430101197937859 1106 46-47 -0.6570807719959006 1106 48-49 -0.29365325978226764 1106 50-51 0.08379405311881527 1106 52-53 0.2150765807993622 1106 54-55 0.3510664987926617 1106 56-57 0.131383981353423 1106 58-59 0.07070497363579165 1106 60-61 0.28427110531314526 1106 62-63 0.033102105694069905 1106 64-65 0.1538899438954786 1106 66-67 0.08076541632308931 1106 68-69 -0.3031443347606242 1106 70-71 -0.045361722079007905 1106 72-73 0.3937270719006598 1106 74-75 0.4214676775142827 1106 76-77 0.35092415086282713 1106 78-79 0.6160350859994814 1106 80-81 0.0700351843050413 1106 82-83 0.5374652344553326 1106 84-85 0.27744661465487397 1106 86-87 0.5793845500371937 1106 88-89 0.8592053223971234 1106 90-91 0.7643371843199809 1106 92-93 0.8758304145473126 1106 94-95 0.9524024366303578 1106 96-97 0.7831055193483252 1106 98-99 0.796899002021906 1106 100-101 0.9116331691224353 1107 1 0.09849516693542526 1107 2 0.15118162784712297 1107 3 0.14394331313535247 1107 4 0.09726886214060926 1107 5 0.06118599032102168 1107 6 0.12033561024664152 1107 7 0.19019457550925978 1107 8 0.15264560470620836 1107 9 0.16311039642558 1107 10-11 0.18961115928886585 1107 12-13 0.23266576651278825 1107 14-15 0.27542082456782424 1107 16-17 0.3372955708415475 1107 18-19 0.27309315734704853 1107 20-21 0.3053979490291212 1107 22-23 0.24783776445936923 1107 24-25 0.1264406556055988 1107 26-27 0.20110803583472858 1107 28-29 0.23851779425303477 1107 30-31 0.3103943749780669 1107 32-33 0.3369446765661266 1107 34-35 0.25096884175350453 1107 36-37 0.33834152152109453 1107 38-39 0.19133208381071398 1107 40-41 0.38569582489673593 1107 42-43 0.34623853372978175 1107 44-45 0.26112586954885586 1107 46-47 0.26456323641044577 1107 48-49 0.39837142703353123 1107 50-51 0.5175988012511752 1107 52-53 0.4365194991908723 1107 54-55 0.23249289178606602 1107 56-57 0.34209818032893224 1107 58-59 0.2413946893917185 1107 60-61 0.21773234943069042 1107 62-63 0.39551847751656055 1107 64-65 0.279733460053734 1107 66-67 0.347024046554651 1107 68-69 0.5835090292533813 1107 70-71 0.6993885702675833 1107 72-73 0.6016605548479248 1107 74-75 0.43382516662524395 1107 76-77 0.5435299136691043 1107 78-79 0.7964308894371541 1107 80-81 0.7732310505784987 1107 82-83 0.9672374599014475 1107 84-85 1.0261068327301892 1107 86-87 1.1170853752219436 1107 88-89 0.9024072500501923 1107 90-91 0.7592339729256175 1107 92-93 0.9906548024797424 1107 94-95 1.0316414105261202 1107 96-97 1.1306414109317906 1107 98-99 1.37394892908382 1107 100-101 1.2103515723080456 1108 1 0.14339889837370023 1108 2 0.14439835410636448 1108 3 0.11097153162553752 1108 4 0.1894835811201787 1108 5 0.09462407088311409 1108 6 0.1399390468808548 1108 7 0.12862167330018082 1108 8 0.012360759246845987 1108 9 0.08011783807889117 1108 10-11 0.09780011550088119 1108 12-13 0.24506643882011048 1108 14-15 0.28045114038339847 1108 16-17 0.2550764363058491 1108 18-19 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2316 64-65 -0.6693379226786362 2316 66-67 -0.7933096323346795 2316 68-69 -0.7359506115501269 2316 70-71 -0.7466322431569843 2316 72-73 -0.8493069650989078 2316 74-75 -0.789568802329736 2316 76-77 -0.8044587269450467 2316 78-79 -0.9841452287876926 2316 80-81 -0.992973794131288 2316 82-83 -1.035998044491187 2316 84-85 -1.240581839492389 2316 86-87 -1.3513745968928923 2316 88-89 -1.35596032574556 2316 90-91 -1.3293182506056382 2316 92-93 -1.4009842897026594 2316 94-95 -1.3119795201959192 2316 96-97 -1.3769048246814002 2316 98-99 -1.3371828367957264 2316 100-101 -1.7555956895106721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 33.0 5 64.0 6 138.0 7 372.0 8 908.0 9 1612.0 10 2580.0 11 4125.0 12 5677.0 13 7831.0 14 10492.0 15 13820.0 16 17124.0 17 21115.0 18 19980.0 19 17806.0 20 17750.0 21 18738.0 22 20502.0 23 23234.0 24 26518.0 25 31129.0 26 36369.0 27 43406.0 28 51932.0 29 61132.0 30 72000.0 31 85381.0 32 102643.0 33 126099.0 34 158289.0 35 203908.0 36 264584.0 37 290922.0 38 136871.0 39 5567.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.98773580686658 19.26641078996451 15.471298052513477 23.274555350655433 2 16.031173671355177 20.29805019792807 39.458750947528 24.21202518318875 3 19.395400129007683 23.64691881039343 29.977818254902765 26.97986280569612 4 13.448794919241253 17.113151687754943 36.34288281813537 33.095170574868426 5 13.85564713815064 37.10100468311715 34.23981328590385 14.803534892828363 6 32.69018899730725 36.22558876854485 16.827821043404878 14.256401190743015 7 29.009681889783668 32.391450978659016 20.06453540924162 18.5343317223157 8 26.536490969988353 35.32732279595215 19.23061543819995 18.905570795859546 9 26.199345280749547 16.783468075859883 19.780160807129306 37.23702583626126 10-11 25.117011862182753 26.526020933758872 27.132572756229145 21.22439444782923 12-13 26.05592156837986 23.701347214812525 27.465298932687666 22.777432284119953 14-15 22.545250023412894 25.341144296040014 25.66148005273952 26.452125627807575 16-17 22.307569678354174 28.40010480558879 26.749153715915824 22.543171800141216 18-19 22.336954177018324 26.82560076436526 29.010050182515357 21.827394876101064 20-21 22.662051432606113 26.029930624172 29.38473542376288 21.923282519459008 22-23 22.446126665340813 25.951694725311498 29.265040285963522 22.337138323384167 24-25 22.41978595557052 26.173350731897248 29.187836088690585 22.219027223841646 26-27 21.919481431692596 26.62432077995704 28.95366900494182 22.50252878340855 28-29 22.109115283405576 26.818954157754188 28.936587537115404 22.135343021724825 30-31 22.256357102644913 26.363442647964096 28.863061195498336 22.517139053892656 32-33 21.89869831339234 26.40739389350851 28.81354505394077 22.88036273915838 34-35 22.65434315609736 26.543694658978943 28.668710938168022 22.133251246755673 36-37 22.714532528721783 26.099521474697834 28.6430258081594 22.542920188420982 38-39 22.2247682407024 26.45323431747617 28.617662797127814 22.70433464469362 40-41 22.77609252273779 26.26237382180855 28.610693633787225 22.35084002166644 42-43 22.4008033123745 26.29869403863613 28.442693095791107 22.85780955319827 44-45 22.429015484062766 26.584311254523474 28.632006535113742 22.354666726300017 46-47 22.83422828467134 26.782431594886415 27.83980002756934 22.543540092872906 48-49 22.52625664110715 26.913570113991863 27.77495420016815 22.785219044732834 50-51 22.72499121354146 27.066238322428305 27.698258281894844 22.510512182135393 52-53 23.084983021705067 26.741945701024168 27.65360174507619 22.519469532194574 54-55 22.575511046980413 26.92214302344088 27.859357345515246 22.642988584063463 56-57 22.678966591640176 27.00943145072612 27.65349651858142 22.658105439052285 58-59 22.61974803182756 26.730018313476194 27.60403948974866 23.046194164947586 60-61 22.35697351764806 26.961000956508833 27.92748000433533 22.75454552150777 62-63 22.40234866239478 26.996015755969786 27.95826962155996 22.643365960075474 64-65 22.122849996145497 26.959870278200427 27.806177150872102 23.11110257478197 66-67 22.12133887911099 27.02898379149139 27.739227432131308 23.11044989726631 68-69 22.545183112273236 26.714598175441846 27.833996938985727 22.906221773299187 70-71 22.373605189561633 26.826712699762687 27.51192150732784 23.287760603347834 72-73 22.398219565835024 26.864799595929835 27.490818978670568 23.246161859564573 74-75 22.367148360207132 27.180198290742226 27.292736706942282 23.159916642108367 76-77 22.366193579902742 26.87648508571254 27.599117133980656 23.158204200404057 78-79 22.51235205948356 27.09171890986102 27.46862250590499 22.92730652475043 80-81 22.601280776433548 27.13591455105068 27.233905499781468 23.028899172734306 82-83 22.544437029208545 27.196535564360268 27.306193920816508 22.952833485614676 84-85 22.48323939959939 27.213410900908748 27.264006457708508 23.039343241783353 86-87 22.58791921306217 27.192596819593785 27.049843447486044 23.169640519858003 88-89 22.41933797996558 27.32505161047591 26.863433717826226 23.392176691732292 90-91 22.375912959025285 27.261455782352307 27.004431355822266 23.358199902800138 92-93 22.493333606988305 26.928386072435416 27.147310050557756 23.43097027001852 94-95 22.21667292497581 26.835962919584105 27.228749663511724 23.71861449192836 96-97 22.451460877771442 26.55515065898255 27.27183250598112 23.721555957264886 98-99 22.141017333165582 26.70703544278876 26.99558814162376 24.156359082421893 100-101 22.36026380852176 26.51727129144569 27.256433996432843 23.866030903599704 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 315.0 1 224.0 2 192.5 3 279.0 4 253.5 5 223.0 6 396.0 7 881.5 8 1437.5 9 1685.0 10 1457.0 11 1199.5 12 1265.5 13 1392.5 14 1517.5 15 1740.0 16 1981.5 17 2149.0 18 2359.5 19 2641.0 20 3023.5 21 3518.5 22 4109.5 23 4939.5 24 6206.0 25 7997.5 26 10292.5 27 13002.0 28 15384.5 29 18032.5 30 21291.0 31 25584.5 32 31430.5 33 34360.5 34 35072.5 35 37671.5 36 41896.0 37 46099.0 38 48616.0 39 50812.5 40 54161.0 41 57874.0 42 60477.5 43 62986.0 44 65764.0 45 68919.5 46 69889.5 47 70897.0 48 72169.5 49 70879.5 50 70240.0 51 68709.5 52 66558.5 53 66653.0 54 67155.5 55 62966.5 56 57196.5 57 52577.5 58 47872.5 59 42318.0 60 34547.0 61 28482.5 62 24395.5 63 18453.5 64 13091.0 65 10125.0 66 7906.0 67 6369.5 68 5033.0 69 3657.5 70 2569.5 71 1783.5 72 1298.5 73 1072.0 74 874.0 75 675.5 76 476.5 77 292.5 78 190.5 79 132.0 80 82.5 81 49.0 82 27.5 83 16.0 84 9.0 85 4.5 86 2.0 87 2.5 88 3.0 89 1.5 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.6829273169032686E-4 2 0.05166620893141443 3 0.0 4 5.261324738433241E-5 5 0.04519477950314154 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006418816180888553 26-27 0.0023412895086027917 28-29 1.3153311846083102E-4 30-31 0.0016836239162986372 32-33 6.57665592304155E-4 34-35 0.012074740274704288 36-37 0.02362334807556525 38-39 0.004787805511974249 40-41 0.0013942510556848088 42-43 0.001657317292606471 44-45 0.00839181295780102 46-47 0.0 48-49 0.0 50-51 5.261324738433241E-4 52-53 0.0 54-55 0.001552090797837806 56-57 0.0 58-59 0.022202790396188273 60-61 0.0 62-63 0.009259931539642503 64-65 0.015126308622995566 66-67 0.006155749943966891 68-69 0.016389026560219542 70-71 2.6306623692166206E-5 72-73 1.0522649476866483E-4 74-75 0.022202790396188273 76-77 0.049456452541272455 78-79 0.030200003998606804 80-81 0.025596344852477717 82-83 0.03525087574750271 84-85 0.01565244109683889 86-87 0.009549304400256331 88-89 0.08315523749093737 90-91 0.023860107688794745 92-93 0.024149480549408575 94-95 0.027437808510929352 96-97 0.03817091097733316 98-99 0.053086766610791404 100-101 0.014126656922693251 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1900662.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.53244170612809 #Duplication Level Percentage of deduplicated Percentage of total 1 79.47583238962378 47.31390358781285 2 12.143894429446991 14.459113744128535 3 3.6049128898887255 6.438277994189109 4 1.572176212080598 3.7438195478979788 5 0.8380013781794194 2.4944134098060644 6 0.5053500632610572 1.805083390936622 7 0.34515951200819006 1.4383731969560236 8 0.23858634989125843 1.1362902373423378 9 0.1833839744418903 0.9825566191469928 >10 0.9972451658429727 10.68038797261977 >50 0.05876916617669169 2.400429933285205 >100 0.031738903631194464 3.5494920855555634 >500 0.0027399380597225823 1.1592229139683172 >1k 0.002121242368817483 2.100029309581476 >5k 8.838509870072845E-5 0.2986060567731923 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5675 0.2985801789060864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4920 0.25885717713091544 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3226 0.16973033606185634 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2092 0.11006691352802339 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2068 0.10880419559079942 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2017 0.10612091997419847 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2003 0.10538433451081781 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1979 0.10412161657359384 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0522649476866481E-4 2 0.0 0.0 0.0 0.0 1.0522649476866481E-4 3 0.0 0.0 0.0 0.0 1.0522649476866481E-4 4 0.0 0.0 0.0 0.0 1.0522649476866481E-4 5 0.0 0.0 0.0 0.0 1.0522649476866481E-4 6 0.0 0.0 0.0 0.0 1.0522649476866481E-4 7 0.0 0.0 0.0 0.0 1.0522649476866481E-4 8 0.0 0.0 0.0 0.0 1.0522649476866481E-4 9 0.0 0.0 0.0 0.0 1.0522649476866481E-4 10-11 0.0 0.0 0.0 0.0 1.0522649476866481E-4 12-13 0.0 0.0 0.0 2.6306623692166203E-5 3.9459935538249305E-4 14-15 0.0 0.0 0.0 7.89198710764986E-5 4.4721260276682544E-4 16-17 0.0 0.0 0.0 1.578397421529972E-4 4.998258501511579E-4 18-19 0.0 0.0 0.0 1.578397421529972E-4 5.524390975354903E-4 20-21 0.0 0.0 0.0 1.578397421529972E-4 5.787457212276565E-4 22-23 0.0 0.0 0.0 4.7351922645899163E-4 6.313589686119888E-4 24-25 0.0 0.0 0.0 8.418119581493185E-4 6.313589686119888E-4 26-27 0.0 0.0 0.0 0.001552090797837806 7.365854633806536E-4 28-29 0.0 0.0 0.0 0.0033146345852129414 7.891987107649861E-4 30-31 0.0 0.0 0.0 0.009733450766101496 8.155053344571523E-4 32-33 0.0 0.0 0.0 0.025201745497095224 8.418119581493185E-4 34-35 0.0 0.0 0.0 0.047509762388052164 8.418119581493185E-4 36-37 0.0 0.0 0.0 0.08636464558138164 8.418119581493185E-4 38-39 0.0 0.0 0.0 0.14379200510138046 8.681185818414847E-4 40-41 0.0 0.0 0.0 0.2057177972727397 8.944252055336509E-4 42-43 0.0 0.0 0.0 0.26866954776809343 8.944252055336509E-4 44-45 0.0 2.6306623692166203E-5 0.0 0.35264029059348795 8.944252055336509E-4 46-47 0.0 5.2613247384332406E-5 0.0 0.45442061765847896 9.207318292258171E-4 48-49 0.0 5.2613247384332406E-5 0.0 0.558884220340071 9.996517003023158E-4 50-51 0.0 5.2613247384332406E-5 0.0 0.6744229115960649 0.0010522649476866481 52-53 0.0 5.2613247384332406E-5 0.0 0.788541045172682 0.0010522649476866481 54-55 0.0 5.2613247384332406E-5 0.0 0.9096830472751073 0.0010522649476866481 56-57 0.0 5.2613247384332406E-5 0.0 1.055106063045402 0.0010522649476866481 58-59 0.0 5.2613247384332406E-5 0.0 1.2144715893725448 0.0010522649476866481 60-61 0.0 5.2613247384332406E-5 0.0 1.3925674317685102 0.0010522649476866481 62-63 0.0 5.2613247384332406E-5 0.0 1.5648495103285067 0.0010522649476866481 64-65 0.0 5.2613247384332406E-5 0.0 1.7489169563025935 0.0010785715713788144 66-67 0.0 5.2613247384332406E-5 0.0 1.9609220366377609 0.0011048781950709805 68-69 0.0 5.2613247384332406E-5 0.0 2.197576423372488 0.0011048781950709805 70-71 0.0 5.2613247384332406E-5 0.0 2.446647536489918 0.0011048781950709805 72-73 0.0 5.2613247384332406E-5 0.0 2.7130021013731005 0.0011311848187631466 74-75 0.0 5.2613247384332406E-5 0.0 2.9760420316710703 0.001157491442455313 76-77 0.0 5.2613247384332406E-5 0.0 3.2680192480304227 0.001157491442455313 78-79 0.0 5.2613247384332406E-5 0.0 3.6015609298233984 0.0011837980661474792 80-81 0.0 5.2613247384332406E-5 0.0 3.95509564562242 0.0012101046898396453 82-83 0.0 5.2613247384332406E-5 0.0 4.319258237393077 0.0012627179372239777 84-85 0.0 5.2613247384332406E-5 0.0 4.683657588776963 0.00131533118460831 86-87 0.0 5.2613247384332406E-5 0.0 5.0620783705887735 0.0013416378083004762 88-89 0.0 5.2613247384332406E-5 0.0 5.466884695963827 0.0013679444319926425 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3325 0.0 51.028145 1 GTATCAA 5950 0.0 41.855015 1 ATCAACG 7665 0.0 32.41027 3 TATCAAC 7750 0.0 32.37915 2 TCAACGC 7900 0.0 31.506294 4 CAACGCA 7990 0.0 31.091955 5 AACGCAG 8320 0.0 29.846958 6 GTGGTAT 1590 0.0 28.69507 1 TGGTATC 1680 0.0 26.02626 2 ACGCAGA 9595 0.0 25.6829 7 CGCAGAG 9685 0.0 25.346186 8 TAAGGTG 1175 0.0 25.063803 5 GTACATG 6120 0.0 23.996078 1 TACATGG 6120 0.0 23.530136 2 GTAAGGT 1125 0.0 23.222197 4 GCAGAGT 10480 0.0 22.698553 9 ACATGGG 6205 0.0 22.506018 3 GGTAAGG 1200 0.0 20.979143 3 AGAGTAC 9540 0.0 20.82902 10-11 AGGTAAG 1100 0.0 20.738712 2 >>END_MODULE