FastQCFastQC Report
Thu 26 May 2016
SRR938610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888782
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60540.320524020241616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46230.244760909411462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.17222739310306853No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21370.11314169660659622No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20830.11028271129225077No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19830.10498829404346292No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19450.10297641548892354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33300.052.48581
GTATCAA57800.040.6199231
ATCAACG73900.031.6370563
TATCAAC74350.031.5734022
TCAACGC77000.030.3633564
CAACGCA77200.030.2846935
AACGCAG79600.029.366926
GTGGTAT15500.028.2667681
TGGTATC15150.028.2296352
ACGCAGA90350.025.763527
CGCAGAG91050.025.4611038
GCAGAGT98950.022.8996269
GTACATG63550.022.8562151
TACATGG63100.022.291422
CGATACC1651.8189894E-1221.6073994-95
TAAGGTG10750.021.2181825
GAGTACT62600.021.05647712-13
ACATGGG64600.020.9646553
AGTACTT64150.020.84389112-13
CAGAGTA95950.020.62034210-11
Produced by FastQC (version 0.11.5)