##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938610_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1888782 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.051550681867997 31.0 31.0 34.0 28.0 34.0 2 31.153021365091366 31.0 31.0 34.0 27.0 34.0 3 31.212253187503904 31.0 31.0 34.0 27.0 34.0 4 34.841727631881284 37.0 35.0 37.0 32.0 37.0 5 34.85796878623367 37.0 35.0 37.0 32.0 37.0 6 34.889371563261406 37.0 35.0 37.0 32.0 37.0 7 34.81346709149071 37.0 35.0 37.0 32.0 37.0 8 34.77283826296523 37.0 35.0 37.0 32.0 37.0 9 36.27393738398608 39.0 35.0 39.0 32.0 39.0 10-11 36.12121224153979 39.0 35.0 39.0 31.0 39.0 12-13 35.97370739450079 38.0 35.0 39.0 30.0 39.0 14-15 36.95838588042453 39.0 36.0 41.0 31.0 41.0 16-17 36.80558873390365 39.0 36.0 41.0 30.5 41.0 18-19 36.805901104521325 39.0 36.0 41.0 30.5 41.0 20-21 36.88693613132696 39.0 36.0 41.0 30.0 41.0 22-23 36.81357059734792 39.0 36.0 41.0 30.0 41.0 24-25 36.693923650267735 39.0 36.0 41.0 30.0 41.0 26-27 36.498492944130135 39.0 36.0 41.0 30.0 41.0 28-29 36.30518688763446 39.0 35.5 41.0 29.0 41.0 30-31 36.06937327865259 39.0 35.0 40.0 28.5 41.0 32-33 35.77388946950998 38.0 35.0 40.0 27.0 41.0 34-35 35.51761373202413 38.0 34.5 40.0 27.0 41.0 36-37 35.21036758080075 38.0 34.0 40.0 25.5 41.0 38-39 34.947241661557555 38.0 34.0 40.0 25.0 41.0 40-41 34.857894929113044 38.0 34.0 40.0 24.5 41.0 42-43 34.664575107132535 38.0 33.5 40.0 24.0 41.0 44-45 34.36219531952338 38.0 33.0 40.0 23.5 41.0 46-47 33.97968240908691 38.0 33.0 40.0 22.5 41.0 48-49 33.59895424670502 38.0 33.0 40.0 20.0 41.0 50-51 32.845517375747974 37.0 31.5 39.5 18.0 40.5 52-53 32.892307847067585 37.0 31.5 39.5 18.0 40.0 54-55 33.013607975933695 37.0 32.0 40.0 17.0 41.0 56-57 32.71043296685377 37.0 31.5 40.0 13.5 41.0 58-59 32.36889063957619 36.5 31.0 39.5 10.5 41.0 60-61 31.9050565390818 36.0 31.0 39.0 8.0 41.0 62-63 31.377257671875313 35.5 30.0 39.0 7.0 40.0 64-65 30.881713453431892 35.0 29.0 38.5 2.0 40.0 66-67 30.3370322779442 35.0 28.5 38.0 2.0 40.0 68-69 29.769597550167248 34.0 28.0 37.5 2.0 40.0 70-71 29.141261934940083 34.0 27.5 37.0 2.0 39.0 72-73 28.582242948100948 34.0 26.0 36.0 2.0 39.0 74-75 27.962165564898438 33.5 26.0 36.0 2.0 39.0 76-77 27.227052936760305 33.0 24.5 35.0 2.0 38.0 78-79 26.44946372847687 32.0 22.5 35.0 2.0 37.0 80-81 25.79348807856068 32.0 20.5 35.0 2.0 36.5 82-83 25.125736585799736 31.0 18.5 35.0 2.0 36.0 84-85 24.468971802992616 31.0 15.0 34.0 2.0 35.5 86-87 23.795461572590167 30.5 7.5 34.0 2.0 35.0 88-89 23.014715568022144 30.0 2.0 34.0 2.0 35.0 90-91 22.18933894965115 29.0 2.0 34.0 2.0 35.0 92-93 21.345092498763755 29.0 2.0 34.0 2.0 35.0 94-95 20.387757030721385 28.0 2.0 34.0 2.0 35.0 96-97 19.168074981654843 26.0 2.0 33.5 2.0 35.0 98-99 17.71605325548422 24.0 2.0 33.0 2.0 34.0 100-101 14.328881257868828 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5783882523364952 1101 2 0.5655497665641391 1101 3 0.6005825973225996 1101 4 0.5548381416147876 1101 5 0.5818516718341087 1101 6 0.5432800616520126 1101 7 0.3004289558995339 1101 8 0.38911002798630534 1101 9 0.3713818961483213 1101 10-11 0.6197116729219161 1101 12-13 0.5422200550191505 1101 14-15 0.7298630321896837 1101 16-17 0.5699645696532016 1101 18-19 0.6530731766704534 1101 20-21 0.74501836260049 1101 22-23 0.8216338416978815 1101 24-25 0.6808865134388 1101 26-27 0.7900233445878087 1101 28-29 0.7197351143778405 1101 30-31 0.5876584008377748 1101 32-33 0.7209990615427913 1101 34-35 0.7597864340871183 1101 36-37 0.860260087180734 1101 38-39 0.8483005361064997 1101 40-41 0.6175052385583726 1101 42-43 0.634001082904831 1101 44-45 0.6841609249822724 1101 46-47 0.5730506228231107 1101 48-49 0.8473030099671988 1101 50-51 0.5664185887461102 1101 52-53 0.6149444398692339 1101 54-55 0.6627585794385098 1101 56-57 0.5119906752284251 1101 58-59 0.6209045925589081 1101 60-61 0.529656204416245 1101 62-63 0.5023734087234253 1101 64-65 0.4927659048847026 1101 66-67 0.4027500437074316 1101 68-69 0.5978575909499177 1101 70-71 0.7217776517378063 1101 72-73 0.7621518221262598 1101 74-75 0.7226155769871951 1101 76-77 0.7396616300743553 1101 78-79 0.5215697822757797 1101 80-81 0.5754756828629937 1101 82-83 0.6197102691602083 1101 84-85 0.6936218845090458 1101 86-87 0.7936382745721389 1101 88-89 0.5391524740102298 1101 90-91 0.5871745013507308 1101 92-93 0.6724858249211003 1101 94-95 0.705280479125836 1101 96-97 0.5161495656918476 1101 98-99 0.5317389220350854 1101 100-101 0.3518286704278104 1102 1 0.5571245668068805 1102 2 0.6640018330829456 1102 3 0.6098907514287966 1102 4 0.5982575837329307 1102 5 0.6020315585153142 1102 6 0.6216273987830903 1102 7 0.4633629125782548 1102 8 0.5188394924062294 1102 9 0.7745075192636364 1102 10-11 0.7694243880021432 1102 12-13 0.7469311902623943 1102 14-15 0.7416003006975558 1102 16-17 0.6992581217709528 1102 18-19 0.8023530470931419 1102 20-21 0.5317920593883585 1102 22-23 0.7668619670909251 1102 24-25 0.6495138651718619 1102 26-27 0.5331222178419353 1102 28-29 0.5759875876663259 1102 30-31 0.7525676138948896 1102 32-33 0.571136645404529 1102 34-35 0.6643711823715392 1102 36-37 0.6539220266070203 1102 38-39 0.7797624528322089 1102 40-41 0.7742735362916875 1102 42-43 1.0446855357750735 1102 44-45 0.8827910682719633 1102 46-47 0.8085326878387704 1102 48-49 0.8287000460089544 1102 50-51 0.9073500176856513 1102 52-53 0.9171580522355214 1102 54-55 0.8916519573140391 1102 56-57 1.0314895448013814 1102 58-59 0.9471936935242979 1102 60-61 1.2390217234770553 1102 62-63 1.0480020151231457 1102 64-65 1.055289564093929 1102 66-67 1.099552330943272 1102 68-69 1.0570651263121924 1102 70-71 0.9183771676634862 1102 72-73 0.9950239350892041 1102 74-75 1.0501423191792725 1102 76-77 0.9025139874058716 1102 78-79 0.928750371447606 1102 80-81 1.021381450955193 1102 82-83 1.0215014668607019 1102 84-85 1.0208522922296943 1102 86-87 1.1657081371109825 1102 88-89 1.58820426505455 1102 90-91 1.5055960141290043 1102 92-93 1.477753277518321 1102 94-95 1.4729086990023355 1102 96-97 1.2699868823617386 1102 98-99 1.236850845049478 1102 100-101 1.1289452685326182 1103 1 0.42916118793814206 1103 2 0.5349367474272775 1103 3 0.48669450632991484 1103 4 0.4888897621871422 1103 5 0.5655303286225148 1103 6 0.5053468717593432 1103 7 0.44009120192013995 1103 8 0.40415323180753404 1103 9 0.3250644731663783 1103 10-11 0.4236062494558155 1103 12-13 0.3909863569418732 1103 14-15 0.5957518855496033 1103 16-17 0.5602470971715903 1103 18-19 0.6362947744259841 1103 20-21 0.616592452231032 1103 22-23 0.506781590998294 1103 24-25 0.5972605177998318 1103 26-27 0.6276066043039208 1103 28-29 0.46161734851129665 1103 30-31 0.6940655374622864 1103 32-33 0.6835084782234588 1103 34-35 0.6466322570553302 1103 36-37 0.6196546375195524 1103 38-39 0.5222923415619434 1103 40-41 0.8323482758150078 1103 42-43 0.8012553323795757 1103 44-45 0.9395146579622491 1103 46-47 0.8278145147981988 1103 48-49 0.8875887892889764 1103 50-51 0.9959560739097597 1103 52-53 0.98397444498179 1103 54-55 0.9737786701638242 1103 56-57 1.1229476267711362 1103 58-59 0.9205727279567739 1103 60-61 1.0539318200458574 1103 62-63 1.1117437511925203 1103 64-65 1.1321232943369388 1103 66-67 1.0416688913405743 1103 68-69 0.8968261369782908 1103 70-71 1.0143550491750233 1103 72-73 1.0769819424971807 1103 74-75 1.110255381951255 1103 76-77 0.8776166793120765 1103 78-79 0.877626446756512 1103 80-81 0.7261678653469765 1103 82-83 0.7471204426077875 1103 84-85 1.0440953832146462 1103 86-87 0.9987272565589507 1103 88-89 1.011001341027729 1103 90-91 1.0535815657129532 1103 92-93 0.8218845122764655 1103 94-95 0.5406394149912437 1103 96-97 0.7226097247772962 1103 98-99 0.7443589903590926 1103 100-101 0.6525626747165756 1104 1 0.432574037999796 1104 2 0.5394412204615691 1104 3 0.5274158275022778 1104 4 0.5922740262818849 1104 5 0.6336016084005323 1104 6 0.41327444155626125 1104 7 0.38236734352489066 1104 8 0.5769984844993346 1104 9 0.6423730723608472 1104 10-11 0.5512695184095904 1104 12-13 0.6124967439527822 1104 14-15 0.795431705254245 1104 16-17 0.7448888643548983 1104 18-19 0.800605136518783 1104 20-21 0.8486883799824767 1104 22-23 0.7951458798481426 1104 24-25 0.7482917958545414 1104 26-27 0.6576920245292825 1104 28-29 0.7330036980425731 1104 30-31 0.7619142717036169 1104 32-33 0.7978743238732093 1104 34-35 0.7115641747137715 1104 36-37 0.7032966818295492 1104 38-39 0.8333631159912969 1104 40-41 0.8059740825971602 1104 42-43 0.7867328170007895 1104 44-45 0.8869450065946083 1104 46-47 0.8534050682714209 1104 48-49 0.8244752244034927 1104 50-51 0.6886690102532924 1104 52-53 0.8594704784605582 1104 54-55 1.03938710296584 1104 56-57 0.9527730660693337 1104 58-59 0.7927390202695435 1104 60-61 0.8226994388406403 1104 62-63 1.0008759021962845 1104 64-65 0.9376145654285502 1104 66-67 0.9235509192092728 1104 68-69 1.005825855194015 1104 70-71 1.0761931617792797 1104 72-73 1.017388352274395 1104 74-75 1.0657538431995093 1104 76-77 1.126809751610704 1104 78-79 0.7974906954346288 1104 80-81 1.0991295869324667 1104 82-83 1.1550673705187542 1104 84-85 1.297821601844312 1104 86-87 1.07049142407854 1104 88-89 1.0707981186731566 1104 90-91 1.1586137438471908 1104 92-93 1.0169822809408906 1104 94-95 1.0613971352400036 1104 96-97 0.9265167452811802 1104 98-99 0.7916022493689105 1104 100-101 1.1199665216541526 1105 1 0.5738484053096009 1105 2 0.7124194410516012 1105 3 0.5470328294217062 1105 4 0.45165703545741565 1105 5 0.5188142433430087 1105 6 0.5175282499018934 1105 7 0.5989376689401169 1105 8 0.420172864013594 1105 9 0.637404193728635 1105 10-11 0.6693490360006535 1105 12-13 0.6147867440637285 1105 14-15 0.7689638418110434 1105 16-17 0.6023568831275767 1105 18-19 0.8464303797758177 1105 20-21 0.8042469558331788 1105 22-23 0.7910851238374406 1105 24-25 0.7362368425088519 1105 26-27 0.9165053074175233 1105 28-29 0.8026736784044886 1105 30-31 0.7933682109697955 1105 32-33 0.8322456833388756 1105 34-35 0.9382017910721885 1105 36-37 1.0289568320553286 1105 38-39 0.9186082036969694 1105 40-41 0.8693806796614325 1105 42-43 0.9669574345019853 1105 44-45 0.8256642042069657 1105 46-47 0.7849180882090749 1105 48-49 0.6005234209174546 1105 50-51 0.5776763994978396 1105 52-53 0.6660062430698446 1105 54-55 0.8433721358624098 1105 56-57 0.9579818732499916 1105 58-59 1.0385074018474612 1105 60-61 0.9365523307424262 1105 62-63 1.0297854893527614 1105 64-65 1.031602483015174 1105 66-67 0.9722899768032143 1105 68-69 0.7782562620462627 1105 70-71 0.9915166908647244 1105 72-73 1.0660708255632763 1105 74-75 0.9754469565567376 1105 76-77 1.001260273628656 1105 78-79 1.3113835291988174 1105 80-81 1.1987248982016148 1105 82-83 1.3039559327226549 1105 84-85 1.1005079694647755 1105 86-87 1.2272255111296104 1105 88-89 1.3993743022282032 1105 90-91 1.2358258018516537 1105 92-93 1.283904871392565 1105 94-95 1.1876389388369901 1105 96-97 1.1055960853528504 1105 98-99 1.2881375701797317 1105 100-101 1.0325348943561075 1106 1 0.4582824322306749 1106 2 0.4913157318649901 1106 3 0.13745967373456125 1106 4 0.4017927374065948 1106 5 0.5238228789215924 1106 6 0.03215664587743561 1106 7 0.2691874153556668 1106 8 0.2993844219816282 1106 9 0.4153464587175293 1106 10-11 0.14196465680280568 1106 12-13 0.23650453883852407 1106 14-15 0.516580147976569 1106 16-17 0.2697061717203795 1106 18-19 0.0415314023379878 1106 20-21 0.29018878218951727 1106 22-23 0.3949204173100398 1106 24-25 0.5516383283766615 1106 26-27 0.25026082459272914 1106 28-29 0.17370336834609645 1106 30-31 0.1696505258531502 1106 32-33 0.5245120429627477 1106 34-35 0.3671200135137269 1106 36-37 0.3904065126759946 1106 38-39 0.3381122747554386 1106 40-41 0.5697754489681159 1106 42-43 0.26179116534607516 1106 44-45 0.3948475252037653 1106 46-47 0.1141666557996075 1106 48-49 0.5007852297284856 1106 50-51 0.36561263328899685 1106 52-53 0.5910672404106379 1106 54-55 0.3620633659526007 1106 56-57 -0.07156414204732187 1106 58-59 -0.5039385229818798 1106 60-61 0.5524075271675706 1106 62-63 0.5141674286569824 1106 64-65 0.6367673814443222 1106 66-67 0.14340465180390183 1106 68-69 -0.15507707128707082 1106 70-71 0.045630241869467625 1106 72-73 0.6769297229506712 1106 74-75 0.6722956557370416 1106 76-77 0.5906275461565507 1106 78-79 0.6516116181315645 1106 80-81 0.5026013134769975 1106 82-83 0.7053814885523586 1106 84-85 0.8345348905383325 1106 86-87 0.8842559686782039 1106 88-89 0.817071755417885 1106 90-91 0.9556561058788482 1106 92-93 0.9610301832329036 1106 94-95 0.7897827554885772 1106 96-97 0.7085760453276286 1106 98-99 0.6182099445526212 1106 100-101 0.1748876097658183 1107 1 0.44925998812758294 1107 2 0.5052350226996012 1107 3 0.36388056467158236 1107 4 0.37578947319768474 1107 5 0.521571534835573 1107 6 0.285610733605715 1107 7 0.34425408628927556 1107 8 0.3231928790562151 1107 9 0.3632007213903421 1107 10-11 0.37540813051845134 1107 12-13 0.4809092474065366 1107 14-15 0.49016192387689017 1107 16-17 0.512139287439787 1107 18-19 0.5050744024233254 1107 20-21 0.5197053379157524 1107 22-23 0.4741820233861631 1107 24-25 0.48391986106141616 1107 26-27 0.5923232499212787 1107 28-29 0.6038796996312996 1107 30-31 0.45929092950564865 1107 32-33 0.4658557556270253 1107 34-35 0.5644569097417502 1107 36-37 0.548947325954714 1107 38-39 0.5499642332807895 1107 40-41 0.5453850879850108 1107 42-43 0.44458179727985936 1107 44-45 0.5471252709794925 1107 46-47 0.5795865760078343 1107 48-49 0.5511569868542736 1107 50-51 0.43544136899474495 1107 52-53 0.4754729090400929 1107 54-55 0.5021469979717139 1107 56-57 0.4448073057253339 1107 58-59 0.4442789629372257 1107 60-61 0.5777672835253078 1107 62-63 0.5157227281961525 1107 64-65 0.28373038707668385 1107 66-67 0.17109335721438157 1107 68-69 0.4250808588204542 1107 70-71 0.5133812446003105 1107 72-73 0.5998629240088711 1107 74-75 0.6321080170614977 1107 76-77 0.6084449531372655 1107 78-79 0.7891849561585325 1107 80-81 0.5744352441818954 1107 82-83 0.4921931778338049 1107 84-85 0.4842682068039039 1107 86-87 0.5119718758295946 1107 88-89 0.9212845583706084 1107 90-91 0.9762128881795817 1107 92-93 0.9628689675817661 1107 94-95 0.7394181988236177 1107 96-97 0.561063137834882 1107 98-99 0.6497279617057181 1107 100-101 0.2682642525376213 1108 1 0.3642236444791074 1108 2 0.3722858750935245 1108 3 0.3212362565955793 1108 4 0.32364033707717965 1108 5 0.406901888755435 1108 6 0.34556339661887847 1108 7 0.42182941937102214 1108 8 0.28134333191612626 1108 9 0.3415345127794396 1108 10-11 0.36715210264132025 1108 12-13 0.38144901826926514 1108 14-15 0.5341747590096304 1108 16-17 0.3995790907082579 1108 18-19 0.5589599630015343 1108 20-21 0.5381294346571224 1108 22-23 0.46894685618897114 1108 24-25 0.4842541734034924 1108 26-27 0.5331797048157796 1108 28-29 0.4073168612169624 1108 30-31 0.5015542650814027 1108 32-33 0.35414544034792783 1108 34-35 0.4504907552771442 1108 36-37 0.521466543786893 1108 38-39 0.4796832673437237 1108 40-41 0.4167502103884573 1108 42-43 0.5230295727696372 1108 44-45 0.700495929021038 1108 46-47 0.5085946835948718 1108 48-49 0.573160584301192 1108 50-51 0.538190370159711 1108 52-53 0.5120496019321621 1108 54-55 0.5598045965015643 1108 56-57 0.6954731102900311 1108 58-59 0.6754354375212799 1108 60-61 0.611415154181298 1108 62-63 0.7546411970046023 1108 64-65 0.7303891095523163 1108 66-67 0.5591587142517618 1108 68-69 0.39102330172072897 1108 70-71 0.5198752105701843 1108 72-73 0.6487407215673677 1108 74-75 0.4936109253737726 1108 76-77 0.6848494238982639 1108 78-79 0.6725086669024698 1108 80-81 0.7819427098072005 1108 82-83 0.8408201898608461 1108 84-85 0.8214745041819107 1108 86-87 1.0824092302088815 1108 88-89 0.7656747254857592 1108 90-91 0.9116236924161214 1108 92-93 1.105222188055631 1108 94-95 0.7177271218866572 1108 96-97 0.74391301723632 1108 98-99 0.5328460601043012 1108 100-101 0.37991944730019966 1109 1 0.5133873470314043 1109 2 0.4744529157626296 1109 3 0.5294024584530099 1109 4 0.5081117669195407 1109 5 0.537927532189741 1109 6 0.514169239677102 1109 7 0.4702536635702046 1109 8 0.49250007973607524 1109 9 0.4960634603470808 1109 10-11 0.49879870432125983 1109 12-13 0.35285233693536355 1109 14-15 0.4140010343708411 1109 16-17 0.6997200530650645 1109 18-19 0.6534774059189701 1109 20-21 0.49247719227260234 1109 22-23 0.5808479526672343 1109 24-25 0.5839104429681683 1109 26-27 0.47075592588630855 1109 28-29 0.4662044245353698 1109 30-31 0.4257140581524226 1109 32-33 0.3446047864395254 1109 34-35 0.5587318589278567 1109 36-37 0.52180651669957 1109 38-39 0.5706760987641317 1109 40-41 0.4744649293229912 1109 42-43 0.32194768253681616 1109 44-45 0.4511575016658256 1109 46-47 0.47686406260347525 1109 48-49 0.4077279138574781 1109 50-51 0.5374435625343708 1109 52-53 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-0.8401149550102502 2316 56-57 -0.6149103906421871 2316 58-59 -0.5423741818595254 2316 60-61 -0.8610424155641425 2316 62-63 -0.9110029810438149 2316 64-65 -0.9661556930257156 2316 66-67 -0.9845948911127671 2316 68-69 -0.8551189263013015 2316 70-71 -1.076310422336391 2316 72-73 -0.8864269428921823 2316 74-75 -0.9184529245143018 2316 76-77 -0.5493757490953648 2316 78-79 -0.7384896045663432 2316 80-81 -0.525269475443519 2316 82-83 -0.6086301086084127 2316 84-85 -0.552944613792004 2316 86-87 -0.7959794292367519 2316 88-89 -1.0221831987541954 2316 90-91 -1.0516520453519576 2316 92-93 -0.8844876097987004 2316 94-95 -1.0007734245703652 2316 96-97 -0.8430297256151569 2316 98-99 -0.849475991365356 2316 100-101 -0.6231386937853465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6713.0 3 2446.0 4 2535.0 5 2708.0 6 3285.0 7 4210.0 8 5228.0 9 6615.0 10 8398.0 11 10327.0 12 12639.0 13 14623.0 14 16856.0 15 19305.0 16 22022.0 17 25047.0 18 24195.0 19 22986.0 20 23529.0 21 25610.0 22 27933.0 23 31412.0 24 34629.0 25 38772.0 26 43936.0 27 49380.0 28 56122.0 29 64503.0 30 74426.0 31 86882.0 32 102212.0 33 122344.0 34 150593.0 35 185619.0 36 221424.0 37 223433.0 38 109510.0 39 6375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.360350516120135 18.467765035380488 14.80548689277644 23.36639755572294 2 15.58992580539051 19.517785359502465 40.54107453202626 24.351214303080766 3 19.33442519791811 23.517375372238604 29.48262144963033 27.66557798021296 4 13.031958003803389 16.364267211925053 36.786983721706335 33.81679106256522 5 14.002892470042275 36.89933568545183 34.099996821461495 14.997775023044404 6 32.66055483300178 35.8104360310342 17.14737536388897 14.381633772075048 7 29.151472877331635 32.37290047650901 20.124495063044627 18.35113158311473 8 26.533457052186478 34.99644096449862 19.370103678093713 19.099998305221188 9 26.345550547955295 16.925624159502632 19.86579584209693 36.863029450445154 10-11 25.13801649259348 26.44895468694634 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22.1961670050549 36-37 22.768063104268556 26.06848902206287 28.562075889747625 22.601371983920952 38-39 22.231172097360872 26.442935675552725 28.54311125727475 22.782780969811647 40-41 22.76950459678536 26.247047325666774 28.60211705525066 22.38133102229721 42-43 22.429585594516393 26.236131057534664 28.419655452893256 22.914627895055688 44-45 22.43909154204358 26.56199121615719 28.56790005797654 22.431017183822693 46-47 22.869166363305425 26.816216037329983 27.75166297117517 22.56295462818943 48-49 22.55572611403249 26.85636781750396 27.74630182566994 22.841604242793608 50-51 22.798133222351773 27.06631677823067 27.640736375371837 22.494813624045722 52-53 23.05176805851116 26.683167451817408 27.719651148445116 22.54541334122632 54-55 22.47889476252289 26.93374330810351 27.938550380538942 22.64881154883466 56-57 22.614699823600844 27.01551851899898 27.712325622830917 22.657456034569257 58-59 22.579932043929464 26.647738795376192 27.7038536503487 23.068475510345642 60-61 22.328669492081847 26.933100776671477 27.99273736982228 22.7454923614244 62-63 22.462410692634414 26.884415858086662 28.0929972012525 22.560176248026423 64-65 22.06931734728618 26.951886721023037 27.892507175771865 23.08628875591892 66-67 22.10370486460042 26.941329142809945 27.864447934194096 23.09051805839554 68-69 22.512758698378466 26.671259100922452 27.97938805372301 22.836594146976076 70-71 22.342022366832037 26.76974109771012 27.639429954778617 23.248806580679222 72-73 22.370314160606515 26.802913956521536 27.625131789439795 23.20164009343216 74-75 22.338275235926115 27.091959421926077 27.597914434849653 22.971850907298155 76-77 22.407924751292516 26.727635388580957 27.717435045886717 23.147004814239814 78-79 22.5660096040443 26.895242109119927 27.59734492306413 22.94140336377164 80-81 22.638568334151376 27.006099055782084 27.32023828622833 23.035094323838205 82-83 22.572803839313465 26.993663798512284 27.46192957905321 22.971602783121043 84-85 22.57657163960401 26.984614855142965 27.441549553985674 22.997263951267357 86-87 22.683705562791882 26.946368247715263 27.23239720007201 23.137528989420847 88-89 22.518034589233977 27.019327190597203 27.152413628199497 23.310224591969323 90-91 22.54628500488451 26.98394665781126 27.182531328261067 23.287237009043164 92-93 22.682097944129136 26.572935159773824 27.40200887271976 23.34295802337727 94-95 22.379023609269996 26.479253510885243 27.55133330755921 23.59038957228555 96-97 22.60743123648801 26.12550036067279 27.57343478072048 23.693633622118725 98-99 22.371891362339564 26.287639586684406 27.231487181523917 24.108981869452112 100-101 22.95633265666749 25.72697845244693 27.45633278919116 23.86035610169442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 318.0 1 222.5 2 199.0 3 289.0 4 275.0 5 258.0 6 420.5 7 905.5 8 1525.0 9 1755.0 10 1537.5 11 1325.5 12 1371.0 13 1532.5 14 1697.0 15 1882.5 16 1995.5 17 2183.5 18 2441.0 19 2677.0 20 3058.0 21 3585.0 22 4202.5 23 5128.0 24 6466.0 25 8063.0 26 10284.5 27 13055.5 28 15564.0 29 18097.0 30 21035.0 31 25066.0 32 30473.5 33 33561.5 34 34685.5 35 37092.0 36 41075.5 37 45244.5 38 48093.5 39 50564.5 40 53659.5 41 57376.0 42 60198.0 43 62345.0 44 64915.5 45 68126.5 46 69470.5 47 70277.5 48 70885.0 49 69735.5 50 69057.5 51 67441.0 52 65734.0 53 65677.5 54 65501.5 55 61552.5 56 55865.5 57 51563.5 58 47853.5 59 42308.5 60 34678.0 61 28993.0 62 24735.0 63 19318.5 64 14370.0 65 11191.5 66 8681.0 67 6908.0 68 5554.5 69 4139.0 70 3017.5 71 2223.5 72 1649.0 73 1294.0 74 990.0 75 748.0 76 561.0 77 380.5 78 252.0 79 164.0 80 105.5 81 71.5 82 50.0 83 31.0 84 17.0 85 9.0 86 13.0 87 15.0 88 8.5 89 4.5 90 3.5 91 4.0 92 3.5 93 3.0 94 2.5 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18858714240182298 2 0.04812625279148149 3 0.04659087178933302 4 0.05252061910797541 5 0.05940336153139961 6 0.0628447327431117 7 0.03573731642931794 8 0.03335482866736341 9 0.040396403608251245 10-11 0.04145528705800881 12-13 0.0384109971399558 14-15 0.03330188449487553 16-17 0.031475310544043726 18-19 0.03944340850346943 20-21 0.05013813134602087 22-23 0.046167318409429996 24-25 0.06509486007384653 26-27 0.05127643105451026 28-29 0.04373188647498758 30-31 0.05228237033177996 32-33 0.048390973653920887 34-35 0.048232141136457254 36-37 0.04547904416708757 38-39 0.025810284087840734 40-41 0.023904293878277114 42-43 0.020648227270272587 44-45 0.004606143006445423 46-47 0.011621245861089317 48-49 0.011382997084893863 50-51 0.009529951047818118 52-53 0.00979467191025751 54-55 0.01114474830869841 56-57 0.008868148891719637 58-59 0.011065332049966592 60-61 0.011118276222454471 62-63 0.004235533799030275 64-65 0.007147463285863589 66-67 0.004023757109078761 68-69 0.008921093064207516 70-71 0.021468861943834704 72-73 0.018689292888221087 74-75 0.019748176337978655 76-77 0.011541829602357499 78-79 0.005215000990056026 80-81 0.0068297982509363175 82-83 0.005876803146154506 84-85 0.005797386887422688 86-87 0.008576955943036306 88-89 0.028351604367258903 90-91 0.03549906765312249 92-93 0.021680638633786217 94-95 0.030866452560433126 96-97 0.034096047082193714 98-99 0.020886476046468042 100-101 0.12317461729304917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1888782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.98073536710048 #Duplication Level Percentage of deduplicated Percentage of total 1 81.2012364434658 50.32912347483809 2 11.17570006356965 13.85356216364397 3 3.301087452858213 6.138114835177821 4 1.3990608194327916 3.468592736469704 5 0.7830311333605592 2.4266422730510793 6 0.4699001951058055 1.7474855785081087 7 0.31519512907012825 1.3675218118726291 8 0.22168751030029119 1.0992283928090967 9 0.16711624684698373 0.9322189084229344 >10 0.8810658896045833 9.721971443452496 >50 0.05235227131510507 2.24256630573706 >100 0.02776871477955273 3.180107219779451 >500 0.0028274696353893297 1.2280681718739372 >1k 0.0018849797569262183 1.9432950751733173 >5k 8.568089804210083E-5 0.32150160919026216 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6054 0.320524020241616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4623 0.244760909411462 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3253 0.17222739310306853 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2137 0.11314169660659622 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2083 0.11028271129225077 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1983 0.10498829404346292 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1945 0.10297641548892354 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.294417248787843E-5 2 0.0 0.0 0.0 0.0 5.294417248787843E-5 3 0.0 0.0 0.0 0.0 5.294417248787843E-5 4 0.0 0.0 0.0 0.0 5.294417248787843E-5 5 0.0 0.0 0.0 0.0 5.294417248787843E-5 6 0.0 0.0 0.0 0.0 5.294417248787843E-5 7 0.0 0.0 0.0 0.0 5.294417248787843E-5 8 0.0 0.0 0.0 0.0 5.294417248787843E-5 9 0.0 0.0 0.0 0.0 5.294417248787843E-5 10-11 0.0 0.0 0.0 5.294417248787843E-5 5.294417248787843E-5 12-13 0.0 0.0 0.0 1.0588834497575686E-4 2.1177668995151373E-4 14-15 0.0 0.0 0.0 1.5883251746363528E-4 2.647208624393921E-4 16-17 0.0 0.0 0.0 1.5883251746363528E-4 3.1766503492727056E-4 18-19 0.0 0.0 0.0 1.5883251746363528E-4 3.1766503492727056E-4 20-21 0.0 0.0 0.0 1.853046037075745E-4 3.1766503492727056E-4 22-23 0.0 0.0 0.0 2.9119294868333134E-4 3.1766503492727056E-4 24-25 0.0 0.0 0.0 4.764975523909059E-4 3.1766503492727056E-4 26-27 0.0 0.0 0.0 0.0011118276222454471 3.1766503492727056E-4 28-29 0.0 0.0 0.0 0.003176650349272706 3.1766503492727056E-4 30-31 0.0 0.0 0.0 0.01143594125738174 3.1766503492727056E-4 32-33 0.0 0.0 0.0 0.02763685803867254 3.1766503492727056E-4 34-35 0.0 0.0 0.0 0.05201764946934056 3.70609207415149E-4 36-37 0.0 0.0 0.0 0.09220227638764028 3.70609207415149E-4 38-39 0.0 2.6472086243939216E-5 0.0 0.15136738914284442 3.70609207415149E-4 40-41 0.0 5.294417248787843E-5 0.0 0.2123590758488804 3.70609207415149E-4 42-43 0.0 5.294417248787843E-5 0.0 0.2802599770645845 3.70609207415149E-4 44-45 0.0 5.294417248787843E-5 0.0 0.36234991650703996 4.2355337990302745E-4 46-47 0.0 5.294417248787843E-5 0.0 0.4627850117165454 4.764975523909059E-4 48-49 0.0 5.294417248787843E-5 0.0 0.5699969610044993 4.764975523909059E-4 50-51 0.0 5.294417248787843E-5 0.0 0.6861035312704167 4.764975523909059E-4 52-53 0.0 5.294417248787843E-5 0.0 0.7958832729240325 4.764975523909059E-4 54-55 0.0 5.294417248787843E-5 0.0 0.9186608089234226 4.764975523909059E-4 56-57 0.0 5.294417248787843E-5 0.0 1.0664544664233353 5.559138111227236E-4 58-59 0.0 5.294417248787843E-5 0.0 1.2281989133738038 5.823858973666628E-4 60-61 0.0 5.294417248787843E-5 0.0 1.4100621458696663 5.823858973666628E-4 62-63 0.0 1.0588834497575686E-4 0.0 1.5859426868743984 6.353300698545411E-4 64-65 0.0 1.0588834497575686E-4 0.0 1.766852924265479 6.353300698545411E-4 66-67 0.0 1.0588834497575686E-4 0.0 1.9818327366525095 6.353300698545411E-4 68-69 0.0 1.0588834497575686E-4 0.0 2.2220404472300137 6.353300698545411E-4 70-71 0.0 1.0588834497575686E-4 0.0 2.481016866954471 6.353300698545411E-4 72-73 0.0 1.0588834497575686E-4 0.0 2.746690724498645 6.618021560984804E-4 74-75 0.0 1.0588834497575686E-4 0.0 3.0091349875210587 6.882742423424196E-4 76-77 0.0 1.5883251746363528E-4 0.0 3.294451133058235 6.882742423424196E-4 78-79 0.0 1.5883251746363528E-4 0.0 3.6201107380311757 7.147463285863588E-4 80-81 0.0 2.1177668995151373E-4 0.0 3.9657567681182897 7.676905010742372E-4 82-83 0.0 2.1177668995151373E-4 0.0 4.32583008520835 7.941625873181765E-4 84-85 0.0 2.1177668995151373E-4 0.0 4.684235660865044 7.941625873181765E-4 86-87 0.0 2.1177668995151373E-4 0.0 5.059477483372882 7.941625873181765E-4 88-89 0.0 2.1177668995151373E-4 0.0 5.458888320621437 7.941625873181765E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3330 0.0 52.4858 1 GTATCAA 5780 0.0 40.619923 1 ATCAACG 7390 0.0 31.637056 3 TATCAAC 7435 0.0 31.573402 2 TCAACGC 7700 0.0 30.363356 4 CAACGCA 7720 0.0 30.284693 5 AACGCAG 7960 0.0 29.36692 6 GTGGTAT 1550 0.0 28.266768 1 TGGTATC 1515 0.0 28.229635 2 ACGCAGA 9035 0.0 25.76352 7 CGCAGAG 9105 0.0 25.461103 8 GCAGAGT 9895 0.0 22.899626 9 GTACATG 6355 0.0 22.856215 1 TACATGG 6310 0.0 22.29142 2 CGATACC 165 1.8189894E-12 21.60739 94-95 TAAGGTG 1075 0.0 21.218182 5 GAGTACT 6260 0.0 21.056477 12-13 ACATGGG 6460 0.0 20.964655 3 AGTACTT 6415 0.0 20.843891 12-13 CAGAGTA 9595 0.0 20.620342 10-11 >>END_MODULE