FastQCFastQC Report
Thu 26 May 2016
SRR938610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888782
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56210.2975991935543647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49890.2641384765420255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.1765158710745867No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21560.11414763588386591No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20120.10652367504561142No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20010.10594128914824473No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19620.10387646642121748No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19500.10324113635136294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.057.3299261
GTATCAA58100.040.2767681
ATCAACG76650.030.2651653
TATCAAC78300.029.6431052
TCAACGC78900.029.4020944
CAACGCA80250.028.9074785
GTACCGT1004.9586062E-528.4967696
AACGCAG83600.027.7241276
TGGTATC13800.026.5385532
ATACCGA1151.283168E-424.7797976
ACGCAGA94050.024.6941687
CGCAGAG95250.024.3331978
GTGGTAT15250.024.0152191
GTACATG64200.023.7072181
TACATGG63150.023.4988632
ACATGGG63200.022.866093
AATACCG1252.2446553E-422.8179515
TATAGCG1100.00285613121.6193662
GAGTACT64500.021.57507312-13
GCAGAGT105700.021.4332379
Produced by FastQC (version 0.11.5)