FastQCFastQC Report
Thu 26 May 2016
SRR938609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882485
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58600.3112906610145632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48880.25965678345378584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.17275038048111938No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22450.11925725835796833No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19110.10151475310560243No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18920.10050544891459959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.051.6240231
GTATCAA62800.043.6263661
ATCAACG77800.034.6319243
TATCAAC78350.034.631422
TCAACGC79750.033.725544
CAACGCA80800.033.346095
AACGCAG83850.032.243916
ACGCAGA93300.028.8199027
CGCAGAG95300.028.1652328
TGGTATC16250.027.7807182
GTGGTAT17400.026.5621551
GCAGAGT103750.025.0470739
TAAGGTG11450.024.0710875
GAGTACT64950.022.92789612-13
GTACATG63000.022.5380611
TAGCCCT13150.022.4046974
GTACTTT67200.021.91340314-15
TACATGG63000.021.6478392
GTAAGGT11050.021.5021024
CAGAGTA99850.021.47878610-11
Produced by FastQC (version 0.11.5)