##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1882485 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.095829714446594 31.0 31.0 34.0 28.0 34.0 2 31.239552506394475 31.0 31.0 34.0 28.0 34.0 3 31.29455427267681 31.0 31.0 34.0 27.0 34.0 4 34.95505887165103 37.0 35.0 37.0 32.0 37.0 5 34.96054948645009 37.0 35.0 37.0 32.0 37.0 6 34.96445018154195 37.0 35.0 37.0 32.0 37.0 7 34.884628562777394 37.0 35.0 37.0 32.0 37.0 8 34.838498580333976 37.0 35.0 37.0 32.0 37.0 9 36.32409235664561 39.0 35.0 39.0 32.0 39.0 10-11 36.174825297412724 39.0 35.0 39.0 31.5 39.0 12-13 36.00418622193537 38.5 35.0 39.0 30.0 39.0 14-15 36.981715923367254 39.0 36.0 41.0 31.0 41.0 16-17 36.802051543571395 39.0 36.0 41.0 30.5 41.0 18-19 36.665103838808804 39.0 36.0 40.5 30.0 41.0 20-21 36.644721737490606 39.0 36.0 40.5 30.0 41.0 22-23 36.74044042847619 39.0 36.0 41.0 30.0 41.0 24-25 36.65271064576876 39.0 36.0 41.0 30.0 41.0 26-27 36.4409379091998 39.0 36.0 41.0 29.5 41.0 28-29 36.24663596257075 39.0 35.0 40.5 29.0 41.0 30-31 35.96423583720454 38.5 35.0 40.0 28.0 41.0 32-33 35.64752839996069 38.0 34.5 40.0 27.0 41.0 34-35 35.368798688966976 38.0 34.0 40.0 26.5 41.0 36-37 35.05499220445316 38.0 34.0 40.0 25.0 41.0 38-39 34.708463015641556 38.0 33.5 40.0 24.5 41.0 40-41 34.570460322393004 38.0 33.0 40.0 24.0 41.0 42-43 34.321029915245006 38.0 33.0 40.0 24.0 41.0 44-45 33.9878896777398 38.0 33.0 40.0 23.0 41.0 46-47 33.57610525449074 38.0 32.5 40.0 20.0 41.0 48-49 33.16107883993764 37.5 32.0 40.0 17.5 41.0 50-51 32.358871119823 36.5 30.5 39.5 15.0 40.5 52-53 32.36246344592387 37.0 31.0 39.0 14.5 40.0 54-55 32.39920238408274 37.0 31.0 40.0 11.0 41.0 56-57 32.048280597189354 36.5 31.0 39.5 8.0 41.0 58-59 31.587138808542964 36.0 30.0 39.0 7.0 41.0 60-61 31.074254243725715 35.5 29.0 39.0 2.0 40.5 62-63 30.465616724701658 35.0 28.0 39.0 2.0 40.0 64-65 29.947362395981905 35.0 28.0 38.0 2.0 40.0 66-67 29.363177927048554 34.0 27.5 38.0 2.0 40.0 68-69 28.72115846872618 34.0 26.0 37.0 2.0 39.5 70-71 28.056748128139134 34.0 25.5 36.5 2.0 39.0 72-73 27.397832386446638 33.0 24.5 36.0 2.0 39.0 74-75 26.682398266121645 32.5 22.5 35.5 2.0 38.5 76-77 25.931321365110477 32.0 20.5 35.0 2.0 37.5 78-79 25.124808962621216 31.0 17.0 35.0 2.0 37.0 80-81 24.341206171629523 31.0 9.0 35.0 2.0 36.0 82-83 23.599808497810074 30.5 4.5 34.0 2.0 36.0 84-85 22.849188970961258 30.0 2.0 34.0 2.0 35.0 86-87 22.11494991991968 29.0 2.0 34.0 2.0 35.0 88-89 21.338033237980646 29.0 2.0 34.0 2.0 35.0 90-91 20.516877690924495 28.0 2.0 34.0 2.0 35.0 92-93 19.653229640608025 26.5 2.0 34.0 2.0 35.0 94-95 18.75486816628021 25.0 2.0 33.0 2.0 35.0 96-97 17.694537539475746 22.0 2.0 33.0 2.0 35.0 98-99 16.46193515486179 10.0 2.0 32.0 2.0 34.5 100-101 13.744008850004118 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5911682871814996 1101 2 0.5857143685120754 1101 3 0.5564646190538873 1101 4 0.43526303479228545 1101 5 0.5717472221931814 1101 6 0.33404352324788533 1101 7 0.3634823554440274 1101 8 0.46808560139604793 1101 9 0.4305367313484254 1101 10-11 0.6101986258309253 1101 12-13 0.6086676522288954 1101 14-15 0.7620435981828635 1101 16-17 0.817333817799387 1101 18-19 0.9161789202599948 1101 20-21 0.9682993810570437 1101 22-23 0.7942057403370626 1101 24-25 0.8710989471401831 1101 26-27 0.8335582419110423 1101 28-29 0.7922727254666242 1101 30-31 0.7692941540438767 1101 32-33 0.9640179914358953 1101 34-35 1.088805963563935 1101 36-37 0.9678986209803355 1101 38-39 1.0363221515728966 1101 40-41 1.1957008711018489 1101 42-43 1.306903009268602 1101 44-45 1.2762803159263356 1101 46-47 1.5697340678991267 1101 48-49 1.5781545016436027 1101 50-51 1.4732613049345673 1101 52-53 1.627125619240907 1101 54-55 1.8264991246777456 1101 56-57 1.7606821217040576 1101 58-59 1.9517294573986455 1101 60-61 2.010831707473823 1101 62-63 2.0238345283225847 1101 64-65 2.1384825460227397 1101 66-67 2.181935471581305 1101 68-69 2.3608816567777318 1101 70-71 2.4476394367913166 1101 72-73 2.8070022921780655 1101 74-75 2.9139837376367055 1101 76-77 3.114401738449292 1101 78-79 3.317577633655578 1101 80-81 3.742744222710524 1101 82-83 3.945016454867819 1101 84-85 4.233557608959391 1101 86-87 4.395819909821935 1101 88-89 4.605690577310888 1101 90-91 4.928569499434804 1101 92-93 4.952832086078434 1101 94-95 5.282878483210148 1101 96-97 5.189321647623331 1101 98-99 5.496999291732539 1101 100-101 5.259199202591974 1102 1 0.634263948376109 1102 2 0.6883108407895335 1102 3 0.6185979085755271 1102 4 0.5950545389748498 1102 5 0.5158492304179418 1102 6 0.6377617888839708 1102 7 0.7071108071098493 1102 8 0.7526220881640953 1102 9 0.7257664815021769 1102 10-11 0.7810195348354227 1102 12-13 0.7654145270262802 1102 14-15 1.0576622085842402 1102 16-17 1.0463920174787305 1102 18-19 1.0286486030092021 1102 20-21 1.1567560701142483 1102 22-23 0.9286533447628003 1102 24-25 0.9454787378638088 1102 26-27 1.0742100163790198 1102 28-29 1.1332071671854251 1102 30-31 1.1587168405693262 1102 32-33 1.0217411556724656 1102 34-35 1.2225853767264567 1102 36-37 1.2829688990901644 1102 38-39 1.5099946919020013 1102 40-41 1.6441487978204918 1102 42-43 1.5589845367859354 1102 44-45 1.622391902293593 1102 46-47 1.7568466633201822 1102 48-49 1.7350014634978521 1102 50-51 1.8344234037916252 1102 52-53 1.887175398388436 1102 54-55 1.9677979714104694 1102 56-57 2.0555881626188963 1102 58-59 2.246159612684661 1102 60-61 2.1420568548819183 1102 62-63 2.492990718895893 1102 64-65 2.655898480763458 1102 66-67 2.7875399061303483 1102 68-69 2.9481204311330345 1102 70-71 3.0950538230153093 1102 72-73 3.0976473941755884 1102 74-75 3.091401894435986 1102 76-77 3.3379735622431923 1102 78-79 3.7853182848005673 1102 80-81 4.235532289311852 1102 82-83 4.456481458704491 1102 84-85 4.581575885233665 1102 86-87 4.970393265136654 1102 88-89 5.2016076548695445 1102 90-91 5.430069703700525 1102 92-93 5.443825667287751 1102 94-95 5.575436569898127 1102 96-97 5.673305200613132 1102 98-99 5.863234986050298 1102 100-101 5.737849185564587 1103 1 0.6308005592050172 1103 2 0.5868879489141072 1103 3 0.4757576446769072 1103 4 0.5195525338627789 1103 5 0.5766363758878583 1103 6 0.5969524488819786 1103 7 0.6133390605734661 1103 8 0.5668929965736282 1103 9 0.7122767745724019 1103 10-11 0.6470751096286662 1103 12-13 0.733085067587048 1103 14-15 0.7479601745701956 1103 16-17 1.0380827009557976 1103 18-19 1.1113176303064165 1103 20-21 1.109095690686516 1103 22-23 1.044689303176682 1103 24-25 1.0828789783500241 1103 26-27 0.9299964544474619 1103 28-29 1.092816511913533 1103 30-31 1.0414516395415774 1103 32-33 1.0402003958657104 1103 34-35 1.0537712088229654 1103 36-37 1.0900482459056349 1103 38-39 1.3700803738233915 1103 40-41 1.221146798010146 1103 42-43 1.5140615132762676 1103 44-45 1.4288966936889338 1103 46-47 1.473333934789359 1103 48-49 1.6068637895369235 1103 50-51 1.5877661408688226 1103 52-53 1.6189928324569465 1103 54-55 1.9130610020638343 1103 56-57 2.109783447235138 1103 58-59 2.1246854485923627 1103 60-61 2.2392691174366313 1103 62-63 2.5037857866564686 1103 64-65 2.5186728942185646 1103 66-67 2.3666882856356395 1103 68-69 2.4956644755851123 1103 70-71 2.8098628715739515 1103 72-73 2.999068650090422 1103 74-75 3.366192680827094 1103 76-77 3.4660592793074443 1103 78-79 3.6989865235041854 1103 80-81 4.119424075971683 1103 82-83 4.362026244186129 1103 84-85 4.534465673294967 1103 86-87 4.750778279374053 1103 88-89 5.115547796283188 1103 90-91 5.475372785092901 1103 92-93 5.4844779247770425 1103 94-95 5.705448327607517 1103 96-97 5.824593534729605 1103 98-99 5.910839924591198 1103 100-101 5.989804234214725 1104 1 0.7217102236802795 1104 2 0.6328022076543967 1104 3 0.6964386627339145 1104 4 0.6819368599053206 1104 5 0.584296352096338 1104 6 0.6020204795654749 1104 7 0.6987562553989832 1104 8 0.7627744473289582 1104 9 0.8248678138628307 1104 10-11 0.8625133027116902 1104 12-13 0.8868224534330338 1104 14-15 1.004875518497677 1104 16-17 1.1827330779608474 1104 18-19 1.250811360654609 1104 20-21 1.2279704341590474 1104 22-23 1.1898775418582161 1104 24-25 1.2403091265939281 1104 26-27 1.1957913196536722 1104 28-29 1.155564077411917 1104 30-31 1.3429887108438834 1104 32-33 1.4625908705376034 1104 34-35 1.5664260860281871 1104 36-37 1.6476437532765331 1104 38-39 1.730670097353027 1104 40-41 1.9060474678207129 1104 42-43 1.817983902890596 1104 44-45 1.9789612772397405 1104 46-47 2.0776466935394 1104 48-49 2.059988950852407 1104 50-51 2.1707888403235955 1104 52-53 2.307743708579274 1104 54-55 2.5295620419392932 1104 56-57 2.688827031381315 1104 58-59 2.7592403440369857 1104 60-61 2.919793907540086 1104 62-63 2.9584518334892955 1104 64-65 3.0826794374614472 1104 66-67 3.494310486776122 1104 68-69 3.8168998166677106 1104 70-71 3.8118746549173643 1104 72-73 3.8944868888063624 1104 74-75 3.991359015016574 1104 76-77 4.018727136315103 1104 78-79 4.256542741592394 1104 80-81 4.5626156447784005 1104 82-83 5.034246369705347 1104 84-85 5.136429965359451 1104 86-87 5.0961272026182804 1104 88-89 5.6115021472988005 1104 90-91 5.899392474472403 1104 92-93 6.000792584325371 1104 94-95 5.868180091357907 1104 96-97 6.174075289016152 1104 98-99 6.328659507057967 1104 100-101 6.01812004892532 1105 1 0.5763371616647746 1105 2 0.5709331537431908 1105 3 0.3885413759122116 1105 4 0.43551552445781994 1105 5 0.49139972595116177 1105 6 0.4979551166338325 1105 7 0.4592246750607174 1105 8 0.5054112073712389 1105 9 0.6291104583307643 1105 10-11 0.5849619901676419 1105 12-13 0.5219676921574958 1105 14-15 0.6333710479198018 1105 16-17 0.7580204722525963 1105 18-19 0.9232330704854803 1105 20-21 0.9878547056530564 1105 22-23 0.9212635311112081 1105 24-25 0.9548236394550997 1105 26-27 1.0242466579200595 1105 28-29 1.1698181736064228 1105 30-31 1.0736011579897564 1105 32-33 1.1743365981626894 1105 34-35 1.1940891630190293 1105 36-37 1.2993620628255087 1105 38-39 1.1910322582057447 1105 40-41 1.3889409006959852 1105 42-43 1.6350981779082119 1105 44-45 1.5769952139909762 1105 46-47 1.8072593168656823 1105 48-49 1.673674865228726 1105 50-51 1.5245123031495211 1105 52-53 1.7436046802142258 1105 54-55 1.7560386539900392 1105 56-57 1.9261937949472028 1105 58-59 2.092978341746633 1105 60-61 2.1896472373498135 1105 62-63 2.3869710371538453 1105 64-65 2.6654440384128186 1105 66-67 2.8283380810326406 1105 68-69 2.851620570719856 1105 70-71 3.005277249173883 1105 72-73 3.0571746310544263 1105 74-75 3.2934219774901443 1105 76-77 3.540287213834482 1105 78-79 3.6906055719306643 1105 80-81 4.156827567787563 1105 82-83 4.3353331826017545 1105 84-85 4.479709783893629 1105 86-87 4.711150436409685 1105 88-89 5.056455679715999 1105 90-91 5.449197024331461 1105 92-93 5.2777845240716665 1105 94-95 5.7002603415340865 1105 96-97 6.057288048882256 1105 98-99 6.033396557797925 1105 100-101 5.962148634196636 1106 1 0.3265085751668835 1106 2 0.3202804638697714 1106 3 0.33619432949871 1106 4 0.2834190054704777 1106 5 0.3352486183562249 1106 6 0.0828970997561953 1106 7 0.3456896012210535 1106 8 0.38964775800415197 1106 9 0.3487636756303445 1106 10-11 0.2772286040916896 1106 12-13 0.10562737042753412 1106 14-15 0.6171955468649557 1106 16-17 0.42508930136652623 1106 18-19 0.6325586242829431 1106 20-21 0.5847725590285862 1106 22-23 0.4637444119128915 1106 24-25 0.5827871151934971 1106 26-27 0.5229940207892554 1106 28-29 0.4318500828891203 1106 30-31 0.6819938267661314 1106 32-33 0.8217719884580461 1106 34-35 0.866916826521134 1106 36-37 0.7845710554415462 1106 38-39 0.7589548584167076 1106 40-41 0.8443503152529459 1106 42-43 0.566654744363909 1106 44-45 0.8484779407842282 1106 46-47 1.2527036734057688 1106 48-49 1.1106804032034105 1106 50-51 0.5393956101115549 1106 52-53 1.1156741686108589 1106 54-55 1.2878754155934118 1106 56-57 1.3892673708965688 1106 58-59 0.614520403982926 1106 60-61 1.8372606339742852 1106 62-63 2.28404382196398 1106 64-65 2.323932303180751 1106 66-67 2.4824684871507827 1106 68-69 1.9750646635468634 1106 70-71 2.369125482296244 1106 72-73 2.750149973881431 1106 74-75 2.941401276013355 1106 76-77 3.021168015522246 1106 78-79 3.265727539230241 1106 80-81 3.678264144791779 1106 82-83 3.8131483197325586 1106 84-85 3.8275539294632495 1106 86-87 4.214786380502513 1106 88-89 4.10203189279617 1106 90-91 4.393675531715424 1106 92-93 4.671251032510487 1106 94-95 4.33484631199822 1106 96-97 4.501740184958205 1106 98-99 4.354849616869657 1106 100-101 3.736100374702966 1107 1 0.6104867656418556 1107 2 0.38399717042671 1107 3 0.42975514402815307 1107 4 0.40656688104697025 1107 5 0.36024083228753057 1107 6 0.3727255076011389 1107 7 0.45916621955009873 1107 8 0.3008753757231091 1107 9 0.3476640267500457 1107 10-11 0.5074599889879963 1107 12-13 0.5246453231820922 1107 14-15 0.6919906026046689 1107 16-17 0.7707811522323738 1107 18-19 0.8573813578969975 1107 20-21 0.7692655899638083 1107 22-23 0.6420560509123519 1107 24-25 0.8701234477631559 1107 26-27 0.7410850047350266 1107 28-29 0.9501913409471712 1107 30-31 0.7821719997491101 1107 32-33 0.8123932420791036 1107 34-35 1.0006801534976262 1107 36-37 0.9317461523940977 1107 38-39 1.175943100386398 1107 40-41 1.2445091830518962 1107 42-43 1.34519228601404 1107 44-45 1.3923724363536323 1107 46-47 1.188249690138349 1107 48-49 1.413824321750461 1107 50-51 1.683623200099305 1107 52-53 1.8389506209793112 1107 54-55 1.9532912449910427 1107 56-57 2.163928143615898 1107 58-59 2.3723022063405033 1107 60-61 2.394446908735052 1107 62-63 2.445272772913871 1107 64-65 2.556987737856698 1107 66-67 2.750262320584376 1107 68-69 2.9652475508344516 1107 70-71 3.083498885085575 1107 72-73 3.5193604985131373 1107 74-75 3.597032790179732 1107 76-77 3.681138663109845 1107 78-79 3.8432005226384476 1107 80-81 4.011703336661849 1107 82-83 4.108517902015304 1107 84-85 4.267990770580518 1107 86-87 4.528899056648985 1107 88-89 4.643582301724763 1107 90-91 4.942342194115753 1107 92-93 5.06124161456712 1107 94-95 5.160865540388148 1107 96-97 5.277680859381075 1107 98-99 5.268302397029473 1107 100-101 5.095723688965764 1108 1 0.43649209775626474 1108 2 0.4947206789702321 1108 3 0.4080818232717789 1108 4 0.3533436862472641 1108 5 0.28289558451154306 1108 6 0.31200868205596777 1108 7 0.32018740210410357 1108 8 0.3954260282592301 1108 9 0.4668443340455326 1108 10-11 0.5058053092056554 1108 12-13 0.5258071956051893 1108 14-15 0.5124801653937325 1108 16-17 0.5677817613545102 1108 18-19 0.761451252876995 1108 20-21 0.7913804477485868 1108 22-23 0.6289091647304517 1108 24-25 0.7625649256138587 1108 26-27 0.8938070766889794 1108 28-29 0.6861448007507178 1108 30-31 0.8285882291300197 1108 32-33 0.9668373339514531 1108 34-35 1.050374112985395 1108 36-37 0.9988388644568573 1108 38-39 1.0883279717106191 1108 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0.2255622379777762 2311 42-43 0.6114041577502647 2311 44-45 0.5023660317382692 2311 46-47 0.5099544020693401 2311 48-49 0.706565295403287 2311 50-51 0.6645872460007354 2311 52-53 0.6554974742707529 2311 54-55 0.7003380617207071 2311 56-57 0.7807150439081383 2311 58-59 0.9424126985629364 2311 60-61 0.9591843321129083 2311 62-63 1.1345655924323204 2311 64-65 1.317896890657206 2311 66-67 1.425217626959487 2311 68-69 1.436413795990017 2311 70-71 1.4547518699136965 2311 72-73 1.3978041769812748 2311 74-75 1.5308420990612177 2311 76-77 1.7312525089300763 2311 78-79 2.0351991660703135 2311 80-81 2.0846181174550615 2311 82-83 2.2645063434496358 2311 84-85 2.5191515440765038 2311 86-87 2.7311012105160835 2311 88-89 2.826092784869779 2311 90-91 2.990185049061303 2311 92-93 3.0694187198322673 2311 94-95 3.189976156164178 2311 96-97 3.1040688832546586 2311 98-99 3.3930209343565316 2311 100-101 3.3090613004476754 2312 1 -0.12609339389078045 2312 2 -0.2678129882321194 2312 3 -0.17949874754457795 2312 4 -0.16947675949279528 2312 5 -0.18210721849327882 2312 6 -0.2617208092920933 2312 7 -0.130516837284965 2312 8 -0.03643678645591564 2312 9 -0.1766418564840464 2312 10-11 -0.12496470736321896 2312 12-13 -0.13748639734868107 2312 14-15 -0.16172733170982667 2312 16-17 -0.126528138858518 2312 18-19 -0.026356049884896038 2312 20-21 0.025264042072812742 2312 22-23 -0.09265815098755326 2312 24-25 0.019443624022997597 2312 26-27 0.1485136332287027 2312 28-29 0.2045751180508617 2312 30-31 0.21264822589099452 2312 32-33 0.23697162902688973 2312 34-35 0.0413210303029814 2312 36-37 0.2861849794921767 2312 38-39 0.22314066869956406 2312 40-41 0.37184406427623173 2312 42-43 0.4393207859328996 2312 44-45 0.4986869578181299 2312 46-47 0.32619836007555847 2312 48-49 0.6942805751052674 2312 50-51 0.6465705593223632 2312 52-53 0.9104237388562026 2312 54-55 0.9397925120147264 2312 56-57 0.9561899369225131 2312 58-59 1.0386554250799698 2312 60-61 1.165256805251044 2312 62-63 1.2173259754254566 2312 64-65 1.3719081587831887 2312 66-67 1.38651130634128 2312 68-69 1.6040742744235637 2312 70-71 1.768026091766476 2312 72-73 1.7030484736470193 2312 74-75 1.7099092460086673 2312 76-77 1.889716226180159 2312 78-79 1.9705071923010316 2312 80-81 2.0559305770468193 2312 82-83 2.386425003589409 2312 84-85 2.5639420369800483 2312 86-87 2.8188491246812895 2312 88-89 2.73939163650612 2312 90-91 2.8835122249487455 2312 92-93 2.9864920858000623 2312 94-95 3.0263020082007515 2312 96-97 3.2221742371538618 2312 98-99 3.1061048911312596 2312 100-101 3.052090763826266 2313 1 -0.35473536919942106 2313 2 -0.35176360551606933 2313 3 -0.29900492038408544 2313 4 -0.3067607101100762 2313 5 -0.3954405518266171 2313 6 -0.26468377225505435 2313 7 -0.27619584963064625 2313 8 -0.31544913213492976 2313 9 -0.4200986466075065 2313 10-11 -0.3274338431656929 2313 12-13 -0.2937826936449781 2313 14-15 -0.22641868973452262 2313 16-17 -0.2551701141671572 2313 18-19 -0.13549185235403627 2313 20-21 -0.16263719249509023 2313 22-23 -0.28969518802459504 2313 24-25 -0.21018600560663003 2313 26-27 -0.12506661368487926 2313 28-29 -0.05912858565283585 2313 30-31 0.0862284728045779 2313 32-33 -0.04228763023237292 2313 34-35 -0.0046048956229469695 2313 36-37 0.21803683134402974 2313 38-39 0.25523943413165995 2313 40-41 0.3955477679799344 2313 42-43 0.6141356007477157 2313 44-45 0.5268351059662777 2313 46-47 0.41607490328544117 2313 48-49 0.6903299578213193 2313 50-51 0.8549656210507663 2313 52-53 0.8993126277450969 2313 54-55 0.9871999194221317 2313 56-57 1.091004751737323 2313 58-59 1.0744578942157723 2313 60-61 1.232911126238701 2313 62-63 1.127943259376071 2313 64-65 1.3338834674251636 2313 66-67 1.4976224174523907 2313 68-69 1.7433335336828186 2313 70-71 1.7588902892973373 2313 72-73 1.7749003254988693 2313 74-75 1.9116376410703957 2313 76-77 2.0524322755628788 2313 78-79 1.9729763281035027 2313 80-81 2.3467947745776847 2313 82-83 2.425437349268421 2313 84-85 2.4572753703133827 2313 86-87 2.570700976533143 2313 88-89 2.748033611814762 2313 90-91 2.796104817541334 2313 92-93 2.969208135182779 2313 94-95 3.235684724250138 2313 96-97 3.473532261845218 2313 98-99 3.526104891131258 2313 100-101 3.536041381110218 2314 1 -0.48068276587145675 2314 2 -0.5259342979476322 2314 3 -0.4934332617689492 2314 4 -0.47035706008323785 2314 5 -0.37833910255125147 2314 6 -0.2407224195980504 2314 7 -0.4463837186590922 2314 8 -0.42125696895725895 2314 9 -0.4267545779011215 2314 10-11 -0.47704736973574313 2314 12-13 -0.4311686303062743 2314 14-15 -0.4559141272009697 2314 16-17 -0.30116582001792835 2314 18-19 -0.40328036550486956 2314 20-21 -0.27777406850153596 2314 22-23 -0.4126849894201925 2314 24-25 -0.3644962578878932 2314 26-27 -0.4403215787949222 2314 28-29 -0.5089031428187027 2314 30-31 -0.4069921391116935 2314 32-33 -0.3588684139146068 2314 34-35 -0.45108369326116815 2314 36-37 -0.28710004466240946 2314 38-39 -0.18107296522421024 2314 40-41 -0.04202603770981739 2314 42-43 -0.012520330545889635 2314 44-45 -0.16663778721675726 2314 46-47 -0.011407651733350122 2314 48-49 0.12300306571182062 2314 50-51 0.21165375846354095 2314 52-53 0.25168514519007346 2314 54-55 0.25307216848279523 2314 56-57 0.23208902441580648 2314 58-59 0.7148873091379393 2314 60-61 0.8356164402483657 2314 62-63 0.8661075105837988 2314 64-65 0.9567068705384223 2314 66-67 1.1563234373128317 2314 68-69 1.282447865405846 2314 70-71 1.4910373639081804 2314 72-73 1.5460597457887246 2314 74-75 1.7116322733838665 2314 76-77 1.983312576153324 2314 78-79 2.114243102123897 2314 80-81 2.1833862936329744 2314 82-83 2.2822006342925754 2314 84-85 2.539454651043389 2314 86-87 2.780915683994227 2314 88-89 2.812698131406819 2314 90-91 2.805262090756578 2314 92-93 2.7833627245547596 2314 94-95 2.976060462307032 2314 96-97 2.953177994534432 2314 98-99 2.968375422532226 2314 100-101 2.717726834679725 2315 1 -0.4076872186352887 2315 2 -0.4584730351022124 2315 3 -0.4341306723591529 2315 4 -0.28450852968438056 2315 5 -0.28331182819103873 2315 6 -0.437510473711896 2315 7 -0.3960315627083659 2315 8 -0.500450460926217 2315 9 -0.6317618100971529 2315 10-11 -0.5108193617566883 2315 12-13 -0.5033650907844489 2315 14-15 -0.413943755570088 2315 16-17 -0.474174366299259 2315 18-19 -0.4166901804929992 2315 20-21 -0.2909241389327235 2315 22-23 -0.43020061190900805 2315 24-25 -0.4136272126323064 2315 26-27 -0.32558822466310744 2315 28-29 -0.438196136569708 2315 30-31 -0.35092489386934034 2315 32-33 -0.3807157909436327 2315 34-35 -0.30575744086225143 2315 36-37 -0.09411834731800184 2315 38-39 -0.3002200860538835 2315 40-41 -0.30440089235685264 2315 42-43 -0.0023503503044466356 2315 44-45 0.060877409848771435 2315 46-47 0.24912677446516085 2315 48-49 0.26728726738137 2315 50-51 0.42611200553048434 2315 52-53 0.42138058937926104 2315 54-55 0.4796412714068694 2315 56-57 0.837958920517984 2315 58-59 1.0294055881588982 2315 60-61 1.3640907305004966 2315 62-63 1.4030465426985295 2315 64-65 1.2836512913482387 2315 66-67 1.4443142103542286 2315 68-69 1.6515186463884817 2315 70-71 1.8068881632312852 2315 72-73 1.844004478575627 2315 74-75 1.9182889662377 2315 76-77 2.086907403006041 2315 78-79 2.22535208611853 2315 80-81 2.5810809720602315 2315 82-83 2.8563244986487213 2315 84-85 2.8245901247285943 2315 86-87 2.975362255555151 2315 88-89 3.0323373251824037 2315 90-91 3.2401976154925833 2315 92-93 3.2875784330736266 2315 94-95 3.1068629997206507 2315 96-97 3.34658038824951 2315 98-99 3.4739359413595707 2315 100-101 3.1091408471777218 2316 1 -0.6573973005371521 2316 2 -0.7299269743643713 2316 3 -0.6954737179487722 2316 4 -0.5692805815797186 2316 5 -0.577723656849443 2316 6 -0.40842806449249736 2316 7 -0.44009911362523013 2316 8 -0.48600891566275095 2316 9 -0.3870528153881594 2316 10-11 -0.4616601242412912 2316 12-13 -0.33844699264717093 2316 14-15 -1.1089317964485375 2316 16-17 -0.762651933886417 2316 18-19 -0.6830886729188848 2316 20-21 -0.5516782883855029 2316 22-23 -0.5564599436478019 2316 24-25 -0.8197209286575315 2316 26-27 -0.363730574449292 2316 28-29 -0.3407927155361463 2316 30-31 -0.39451226793616456 2316 32-33 -0.2813101230448254 2316 34-35 -0.15526276810730621 2316 36-37 -0.2148601752516015 2316 38-39 -0.07043281345838892 2316 40-41 -0.12721563469214914 2316 42-43 0.10424891040440087 2316 44-45 0.13525046196154733 2316 46-47 0.29977184223351827 2316 48-49 0.30628125158083463 2316 50-51 0.3618589070308076 2316 52-53 0.6311272734410167 2316 54-55 0.6057556440965826 2316 56-57 0.8861578043333012 2316 58-59 1.1192439588191867 2316 60-61 0.8849557736258156 2316 62-63 1.100444527767749 2316 64-65 1.221355140010992 2316 66-67 1.4997972863852524 2316 68-69 1.7256470801059542 2316 70-71 1.4226379639125923 2316 72-73 1.7899891129164232 2316 74-75 2.129503360671272 2316 76-77 2.1800798317949095 2316 78-79 2.4643986179732806 2316 80-81 2.6376150011969983 2316 82-83 2.8630832143115477 2316 84-85 2.743204678617122 2316 86-87 3.1401716344056254 2316 88-89 3.1030682811873334 2316 90-91 3.2662993042655017 2316 92-93 3.404134568465377 2316 94-95 3.6363984059683823 2316 96-97 3.5660030888365917 2316 98-99 3.345638122993261 2316 100-101 3.0361902057339254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6217.0 3 2221.0 4 2553.0 5 2900.0 6 3415.0 7 4457.0 8 5364.0 9 7014.0 10 9121.0 11 11288.0 12 13619.0 13 16154.0 14 18828.0 15 22018.0 16 24556.0 17 28281.0 18 27264.0 19 26820.0 20 28350.0 21 30713.0 22 34062.0 23 37473.0 24 41514.0 25 46104.0 26 51451.0 27 57783.0 28 63737.0 29 70988.0 30 79125.0 31 90023.0 32 102038.0 33 118584.0 34 137813.0 35 164092.0 36 188574.0 37 192777.0 38 106664.0 39 8530.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37142817561955 18.431943188617687 14.815961364167974 23.38066727159479 2 15.585258936831986 19.535059950865957 40.4808504238781 24.398830688423953 3 19.318981405700946 23.47463824341589 29.55110721855051 27.655273132332653 4 12.960586140390317 16.421050841386332 36.78327505836797 33.83508795985538 5 14.014236630834127 36.90430883453531 34.14904451285889 14.93241002177167 6 32.70579918517475 35.75222508949524 17.168841039978528 14.373134685351483 7 29.121844943939635 32.281470853924226 20.19347467984484 18.4032095222913 8 26.51606413431796 35.05600567097605 19.428406850020433 18.99952334468556 9 26.379258942763446 16.934055439792488 19.848627609784042 36.838058007660024 10-11 25.156799239052745 26.35502546675771 27.12229623272658 21.365879061462962 12-13 26.163247021638057 23.68665915976157 27.355335756869803 22.794758061730565 14-15 22.77897616304109 25.22712714417456 25.716651066551076 26.277245626233274 16-17 22.422189545218465 28.278918152075587 26.642225330522578 22.656666972183366 18-19 22.464146612326445 26.719209126606696 28.944749384953 21.87189487611386 20-21 22.847063937746352 25.949994712287317 29.295283413428454 21.907657936537884 22-23 22.563565317038137 25.848606979199072 29.142416546152255 22.44541115761053 24-25 22.444949959340303 26.05638144642221 29.17455500220572 22.324113592031765 26-27 21.981152331086324 26.470361583578335 28.970611369400277 22.57787471593506 28-29 22.144729262366695 26.780206488860358 28.856786349713136 22.21827789905981 30-31 22.270109177830278 26.320654774699992 28.788229445358343 22.62100660211139 32-33 21.913239678516685 26.368162640862835 28.756065541189884 22.962532139430593 34-35 22.721900817443323 26.501679470604085 28.5781013612719 22.19831835068069 36-37 22.77360991105599 26.0571732830001 28.58146045025714 22.58775635568677 38-39 22.26186456504985 26.441091404763757 28.463399919186294 22.833644111000094 40-41 22.792180294848833 26.230250297935783 28.552980500149566 22.42458890706582 42-43 22.397762282645974 26.311854891606252 28.378938061890025 22.91144476385775 44-45 22.41861843929733 26.59328504504581 28.532422464785217 22.455674050871643 46-47 22.814668293513574 26.822385987226966 27.762392155643877 22.600553563615584 48-49 22.611970708916267 26.8497793286725 27.715782130696294 22.822467831714935 50-51 22.759202134992538 27.08143577026252 27.614015151817 22.545346942927942 52-53 23.01013770340617 26.750032804233363 27.701727441710727 22.538102050649737 54-55 22.510980966325036 26.964647344699276 27.89739198065395 22.626979708321734 56-57 22.60867786694759 27.03401866855153 27.681661910677718 22.67564155382316 58-59 22.619396098552482 26.647734426590063 27.685545603925355 23.047323870932097 60-61 22.349156158491752 26.873355773439922 28.05162074519854 22.72586732286978 62-63 22.47126725299559 26.91096772959702 28.0489887725209 22.568776244886486 64-65 22.154861673727968 26.918146441236235 27.847130543344 23.07986134169179 66-67 22.16368664691202 26.96262695054148 27.782977923169355 23.090708479377145 68-69 22.601227098160948 26.668373773526067 27.937733454389168 22.792665673923818 70-71 22.40121346392415 26.76835536854185 27.593447082294 23.236984085239996 72-73 22.42851029228118 26.83625086766285 27.583908609632875 23.151330230423095 74-75 22.400719561866303 27.110093656204665 27.463977412217726 23.025209369711302 76-77 22.52579771910629 26.74749380873336 27.582620836285194 23.14408763587516 78-79 22.648289828325417 26.892143120678487 27.55232168817015 22.907245362825947 80-81 22.752469719358707 26.96839773910212 27.28991082686823 22.989221714670947 82-83 22.6762243260761 27.013696301254875 27.386200631156164 22.923878741512862 84-85 22.706220063158717 26.977149655453236 27.371513129113595 22.94511715227445 86-87 22.791899596775373 26.964043391336663 27.16139264293677 23.0826643689512 88-89 22.621856035126758 27.049255671064948 27.12039968163188 23.20848861217641 90-91 22.628783492927525 26.982502071700086 27.168191227150523 23.220523208221866 92-93 22.781623729551963 26.565261049510948 27.32896435573076 23.324150865206327 94-95 22.522184092701757 26.448730642966083 27.50468840784353 23.52439685648863 96-97 22.763905614208184 26.15777951163219 27.4748004451348 23.60351442902483 98-99 22.57871539200435 26.263584993072026 27.15678367718189 24.000915937741734 100-101 23.01522895548946 25.766832178367622 27.480162792466196 23.737776073676724 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 332.0 1 239.5 2 212.5 3 298.5 4 270.5 5 248.5 6 405.5 7 948.0 8 1570.5 9 1766.0 10 1594.5 11 1394.5 12 1397.0 13 1571.5 14 1727.5 15 1853.0 16 2009.5 17 2164.5 18 2446.5 19 2803.5 20 3123.5 21 3651.5 22 4408.5 23 5325.0 24 6505.0 25 8181.0 26 10416.0 27 13139.0 28 15629.5 29 18146.5 30 21206.5 31 25266.5 32 30442.5 33 33324.5 34 34599.0 35 37115.0 36 41116.5 37 45240.5 38 47837.0 39 50043.5 40 52942.0 41 56581.0 42 59350.0 43 61524.0 44 63985.5 45 66901.0 46 68388.0 47 69495.5 48 70235.0 49 69096.5 50 68249.0 51 66775.5 52 65154.0 53 64653.0 54 64404.5 55 61319.0 56 56319.5 57 51658.0 58 47790.0 59 42497.5 60 35062.0 61 29526.0 62 25253.5 63 19811.0 64 14858.5 65 11605.0 66 9141.0 67 7308.0 68 5798.5 69 4398.0 70 3235.5 71 2373.0 72 1776.5 73 1403.5 74 1092.5 75 835.5 76 600.5 77 396.5 78 283.0 79 207.5 80 129.5 81 78.5 82 54.5 83 37.5 84 23.0 85 16.5 86 14.0 87 7.5 88 6.5 89 8.5 90 5.5 91 4.0 92 4.0 93 3.5 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3016225892902201 2 0.036866163608209365 3 0.044250020584493364 4 0.04717169061108056 5 0.06061137273338167 6 0.058433400531743944 7 0.031075944828245643 8 0.03304143193704066 9 0.040797137825799405 10-11 0.03293518939061931 12-13 0.03516628286546772 14-15 0.02945574599532002 16-17 0.022709344297564125 18-19 0.027357455703498304 20-21 0.0405846527329567 22-23 0.03301487130043533 24-25 0.05391809230883645 26-27 0.04637487151292042 28-29 0.039150378356268445 30-31 0.03923006026608446 32-33 0.034289781857491555 34-35 0.03410385740125419 36-37 0.03330703830309405 38-39 0.020106401910240986 40-41 0.019548628541528883 42-43 0.02063761464234775 44-45 0.009508707904711061 46-47 0.016812882971179055 48-49 0.01455522885972531 50-51 0.007251053793257317 52-53 0.005630854960331689 54-55 0.007277614429862655 56-57 0.006454234695097171 58-59 0.008393161167286858 60-61 0.007490099522705361 62-63 0.005019960318408912 64-65 0.00849940371370821 66-67 0.003134155119429902 68-69 0.008393161167286858 70-71 0.014342743766882604 72-73 0.01452866822311997 74-75 0.012138210928639537 76-77 0.010438330185897895 78-79 0.006135507055833115 80-81 0.007197932520046641 82-83 0.01195228647240217 84-85 0.004488747586302149 86-87 0.0018858051989790092 88-89 0.020557932732531734 90-91 0.030624414005954892 92-93 0.014608350132935986 94-95 0.023878012308199005 96-97 0.01904397644602746 98-99 0.013864652307986518 100-101 0.10911109517472914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1882485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.892836261864446 #Duplication Level Percentage of deduplicated Percentage of total 1 81.11209000165583 50.202573053300966 2 11.194916040307838 13.857702108961858 3 3.360990998220285 6.240637965913453 4 1.4279581793371858 3.5352152713002605 5 0.7462729211866324 2.3094473858833755 6 0.4670685468987693 1.7344918257763493 7 0.31966896411059925 1.3849653197587999 8 0.23246944241699952 1.1510554508321822 9 0.1669349781160206 0.929887134022151 >10 0.8876533742161635 9.817183609839493 >50 0.05145682149502852 2.1898862214135075 >100 0.027604086181154532 3.120615093039768 >500 0.0029321396343671606 1.255937544967245 >1k 0.0018972668222375728 1.9576185890020674 >5k 8.623940101079877E-5 0.31278342598844244 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5860 0.3112906610145632 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4888 0.25965678345378584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3252 0.17275038048111938 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2245 0.11925725835796833 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1911 0.10151475310560243 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1892 0.10050544891459959 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.3121273210676313E-5 0.0 5 0.0 0.0 0.0 5.3121273210676313E-5 0.0 6 0.0 0.0 0.0 5.3121273210676313E-5 0.0 7 0.0 0.0 0.0 1.0624254642135263E-4 0.0 8 0.0 0.0 0.0 1.0624254642135263E-4 0.0 9 0.0 0.0 0.0 1.0624254642135263E-4 0.0 10-11 0.0 0.0 0.0 1.5936381963202895E-4 0.0 12-13 0.0 0.0 0.0 2.1248509284270525E-4 1.5936381963202895E-4 14-15 0.0 0.0 0.0 2.1248509284270525E-4 1.5936381963202895E-4 16-17 0.0 0.0 0.0 2.1248509284270525E-4 1.5936381963202895E-4 18-19 0.0 0.0 0.0 2.6560636605338155E-4 1.5936381963202895E-4 20-21 0.0 0.0 0.0 3.718489124747342E-4 1.5936381963202895E-4 22-23 0.0 0.0 0.0 4.5153082229074863E-4 1.5936381963202895E-4 24-25 0.0 0.0 0.0 7.436978249494684E-4 1.5936381963202895E-4 26-27 0.0 0.0 0.0 0.0012217892838455552 1.5936381963202895E-4 28-29 0.0 0.0 0.0 0.003638807214931328 1.5936381963202895E-4 30-31 0.0 0.0 0.0 0.010597694005529925 1.5936381963202895E-4 32-33 0.0 0.0 0.0 0.02523260477507125 1.5936381963202895E-4 34-35 0.0 0.0 0.0 0.047862267162819355 1.5936381963202895E-4 36-37 0.0 0.0 0.0 0.08549868923258352 1.5936381963202895E-4 38-39 0.0 0.0 0.0 0.14371960467148476 1.5936381963202895E-4 40-41 0.0 0.0 0.0 0.20775729952695507 1.5936381963202895E-4 42-43 0.0 0.0 0.0 0.2743979367697485 1.5936381963202895E-4 44-45 0.0 0.0 0.0 0.3563375006972167 1.859244562373671E-4 46-47 0.0 0.0 0.0 0.4547712199565999 2.1248509284270525E-4 48-49 0.0 0.0 0.0 0.5601638260065818 2.1248509284270525E-4 50-51 0.0 2.6560636605338157E-5 0.0 0.6734980624015596 2.6560636605338155E-4 52-53 0.0 5.3121273210676313E-5 0.0 0.7842027957726091 3.187276392640579E-4 54-55 0.0 5.3121273210676313E-5 0.0 0.9091971516373305 3.187276392640579E-4 56-57 0.0 5.3121273210676313E-5 0.0 1.056900851799616 3.187276392640579E-4 58-59 0.0 5.3121273210676313E-5 0.0 1.219956599919787 3.187276392640579E-4 60-61 0.0 5.3121273210676313E-5 0.0 1.3977535013559206 3.187276392640579E-4 62-63 0.0 1.0624254642135263E-4 0.0 1.569175850006773 3.718489124747342E-4 64-65 0.0 1.0624254642135263E-4 0.0 1.745990007888509 4.780914588960868E-4 66-67 0.0 1.0624254642135263E-4 0.0 1.953906671235096 4.780914588960868E-4 68-69 0.0 1.0624254642135263E-4 0.0 2.1922352634947955 4.780914588960868E-4 70-71 0.0 1.0624254642135263E-4 0.0 2.445039402704404 4.780914588960868E-4 72-73 0.0 1.0624254642135263E-4 0.0 2.7057586116224033 5.843340053174395E-4 74-75 0.0 1.0624254642135263E-4 0.0 2.9655481982592162 6.905765517387921E-4 76-77 0.0 1.0624254642135263E-4 0.0 3.247462795188275 6.905765517387921E-4 78-79 0.0 1.0624254642135263E-4 0.0 3.572936836150089 6.905765517387921E-4 80-81 0.0 1.3280318302669078E-4 0.0 3.922448253239734 6.905765517387921E-4 82-83 0.0 1.5936381963202895E-4 0.0 4.274721976536334 6.905765517387921E-4 84-85 0.0 1.5936381963202895E-4 0.0 4.626916017923118 6.905765517387921E-4 86-87 0.0 1.5936381963202895E-4 0.0 4.998393081485377 7.436978249494683E-4 88-89 0.0 1.5936381963202895E-4 0.0 5.393801278629047 7.968190981601447E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3415 0.0 51.624023 1 GTATCAA 6280 0.0 43.626366 1 ATCAACG 7780 0.0 34.631924 3 TATCAAC 7835 0.0 34.63142 2 TCAACGC 7975 0.0 33.72554 4 CAACGCA 8080 0.0 33.34609 5 AACGCAG 8385 0.0 32.24391 6 ACGCAGA 9330 0.0 28.819902 7 CGCAGAG 9530 0.0 28.165232 8 TGGTATC 1625 0.0 27.780718 2 GTGGTAT 1740 0.0 26.562155 1 GCAGAGT 10375 0.0 25.047073 9 TAAGGTG 1145 0.0 24.071087 5 GAGTACT 6495 0.0 22.927896 12-13 GTACATG 6300 0.0 22.538061 1 TAGCCCT 1315 0.0 22.404697 4 GTACTTT 6720 0.0 21.913403 14-15 TACATGG 6300 0.0 21.647839 2 GTAAGGT 1105 0.0 21.502102 4 CAGAGTA 9985 0.0 21.478786 10-11 >>END_MODULE