FastQCFastQC Report
Thu 26 May 2016
SRR938609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882485
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56830.3018881956562735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50260.26698751915685914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33100.1758314143273386No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21420.11378576721726866No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20400.10836739734977968No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20130.10693312297309142No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19830.10533948477677113No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19020.10103666164670635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.054.490871
GTATCAA54950.043.2794041
ATCAACG74750.031.5491563
TATCAAC75250.031.4776152
TCAACGC78050.030.2761574
CAACGCA78400.030.0803495
AACGCAG82200.028.716316
ACGCAGA93750.025.0264787
CGCAGAG93750.025.0264788
TGGTATC14250.023.6985872
GTGGTAT15400.023.4731751
TAAGGTG11600.022.5435335
GTAAGGT11350.022.2022654
GTACATG63150.022.0685231
GCAGAGT105050.021.8823229
TACATGG62600.021.2746622
GTACCGT1805.1767565E-621.1087026
AGAGTAC96000.020.7541410-11
ACATGGG62350.020.5894433
GAGTACT66500.020.3905312-13
Produced by FastQC (version 0.11.5)