##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938609_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1882485 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800077557058888 31.0 31.0 34.0 30.0 34.0 2 32.03062600764415 31.0 31.0 34.0 30.0 34.0 3 32.09706106555962 33.0 31.0 34.0 30.0 34.0 4 35.76766667463486 37.0 35.0 37.0 35.0 37.0 5 35.64017030680191 37.0 35.0 37.0 33.0 37.0 6 35.68233691105108 37.0 35.0 37.0 33.0 37.0 7 35.613984706385445 37.0 35.0 37.0 33.0 37.0 8 35.59751286198828 37.0 35.0 37.0 33.0 37.0 9 37.1611667556448 39.0 37.0 39.0 33.0 39.0 10-11 37.100256310143244 39.0 37.0 39.0 33.0 39.0 12-13 36.991651726308575 39.0 37.0 39.0 33.0 39.0 14-15 38.04429118957123 40.0 37.0 41.0 33.0 41.0 16-17 37.95961933295617 40.0 37.0 41.0 33.0 41.0 18-19 37.959338587027254 40.0 37.0 41.0 33.0 41.0 20-21 37.950934270392594 40.0 37.0 41.0 32.5 41.0 22-23 37.97914538495658 40.0 37.0 41.0 33.0 41.0 24-25 37.90641412813382 40.0 37.0 41.0 32.5 41.0 26-27 37.7314424816134 40.0 37.0 41.0 32.0 41.0 28-29 37.59316435456326 39.5 37.0 41.0 32.0 41.0 30-31 37.340865398661876 39.0 36.0 41.0 31.5 41.0 32-33 37.10903991266863 39.0 36.0 41.0 31.0 41.0 34-35 36.94122317043695 39.0 36.0 40.5 30.5 41.0 36-37 36.93757825427559 39.0 36.0 41.0 31.0 41.0 38-39 36.77933848078471 39.0 35.0 41.0 30.0 41.0 40-41 36.595341529945784 39.0 35.0 40.5 30.0 41.0 42-43 36.36375376165016 39.0 35.0 40.0 30.0 41.0 44-45 36.0769310246828 38.5 35.0 40.0 29.5 41.0 46-47 35.70375726765419 38.0 35.0 40.0 28.5 41.0 48-49 35.30362924538575 38.0 34.0 40.0 27.0 41.0 50-51 34.923142548280595 38.0 34.0 40.0 26.0 41.0 52-53 34.39413381779934 38.0 33.0 40.0 24.0 41.0 54-55 34.05874230073546 37.5 33.0 40.0 23.5 41.0 56-57 33.6878293850947 37.0 33.0 40.0 23.0 41.0 58-59 33.22399700396019 36.5 32.0 39.0 22.0 41.0 60-61 32.80842344029301 36.0 31.0 39.0 20.5 40.0 62-63 32.31377593978172 35.5 31.0 39.0 18.5 40.0 64-65 31.812794258652794 35.0 30.5 38.5 17.5 40.0 66-67 31.314673689298985 35.0 30.0 38.0 13.5 40.0 68-69 30.744565826553732 34.0 29.0 37.5 9.5 40.0 70-71 30.17340244410978 34.0 29.0 37.0 7.5 39.0 72-73 29.58446255879861 34.0 28.0 36.0 6.5 39.0 74-75 28.956957691561954 33.5 27.0 36.0 2.0 38.5 76-77 27.70574320645317 31.5 26.0 34.5 2.0 36.5 78-79 28.322893940721972 33.0 27.0 35.0 2.0 37.0 80-81 28.018493108842833 33.0 26.0 35.0 2.0 37.0 82-83 27.52690300321118 33.0 26.0 35.0 2.0 36.0 84-85 26.926631819111442 32.0 25.0 35.0 2.0 36.0 86-87 26.27866862152952 32.0 24.5 35.0 2.0 35.0 88-89 25.52766688711995 31.5 21.0 34.0 2.0 35.0 90-91 24.978599563874347 31.0 19.5 34.0 2.0 35.0 92-93 24.202283683535327 31.0 14.5 34.0 2.0 35.0 94-95 23.408046013646857 31.0 4.5 34.0 2.0 35.0 96-97 22.44170365235314 30.0 2.0 34.0 2.0 35.0 98-99 21.27422954233367 29.5 2.0 34.0 2.0 35.0 100-101 18.17518333479417 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23119169164129616 1101 2 0.262148110091303 1101 3 0.2621245601360087 1101 4 0.16718463095307357 1101 5 0.15763334666368678 1101 6 0.27422091464396914 1101 7 0.3058433286746052 1101 8 0.25120546646561337 1101 9 0.32917943102321345 1101 10-11 0.24918028882288468 1101 12-13 0.40158542028818545 1101 14-15 0.5810729674965174 1101 16-17 0.45183608939645836 1101 18-19 0.6032195885362128 1101 20-21 0.6722421998791148 1101 22-23 0.6020349984730373 1101 24-25 0.5986268705366413 1101 26-27 0.45519039595108524 1101 28-29 0.39405823787238603 1101 30-31 0.5130609316778774 1101 32-33 0.48869787339616977 1101 34-35 0.40169802790368436 1101 36-37 0.5535633796031973 1101 38-39 0.636796728343036 1101 40-41 0.7078159100251895 1101 42-43 0.5181474857625616 1101 44-45 0.5617167885517702 1101 46-47 0.6161769774260861 1101 48-49 0.7185390653584989 1101 50-51 0.8307047750623013 1101 52-53 0.8175993633020084 1101 54-55 0.905661997360653 1101 56-57 1.0642528218662122 1101 58-59 1.0831418885302284 1101 60-61 1.1767255737081044 1101 62-63 1.2241068381292948 1101 64-65 1.201015303218032 1101 66-67 1.203318787971586 1101 68-69 1.3576438004236344 1101 70-71 1.266117102437665 1101 72-73 1.344897879994317 1101 74-75 1.365135924836352 1101 76-77 1.362014884720299 1101 78-79 1.6013635979231218 1101 80-81 2.045498656868869 1101 82-83 2.1308013183322174 1101 84-85 2.336617429425747 1101 86-87 2.4626172101733914 1101 88-89 2.6747704686362717 1101 90-91 2.690789603679775 1101 92-93 3.1433131722345813 1101 94-95 3.3059352626318983 1101 96-97 3.714541490672108 1101 98-99 3.779339406902519 1101 100-101 4.475411908625119 1102 1 0.3748654028001219 1102 2 0.3988509780575882 1102 3 0.3763884135380593 1102 4 0.27824034796982744 1102 5 0.19683505481611974 1102 6 0.22484903961052538 1102 7 0.24669943181337572 1102 8 0.2586956292927809 1102 9 0.3718876482964788 1102 10-11 0.4635916290011721 1102 12-13 0.48545506917146497 1102 14-15 0.5861830727012389 1102 16-17 0.4604207576959922 1102 18-19 0.5601110230355246 1102 20-21 0.5731723800295256 1102 22-23 0.5985459573762881 1102 24-25 0.510816529844071 1102 26-27 0.6705147944687937 1102 28-29 0.5597857228483107 1102 30-31 0.5503450914218533 1102 32-33 0.36448007892462186 1102 34-35 0.6040594214932042 1102 36-37 0.5402192255371077 1102 38-39 0.5962531297286446 1102 40-41 0.6493158121413032 1102 42-43 0.6247568824894003 1102 44-45 0.5016563712202213 1102 46-47 0.5892957341811709 1102 48-49 0.48889340501367684 1102 50-51 0.5643735888795973 1102 52-53 0.6504514604405927 1102 54-55 0.6721060175366986 1102 56-57 0.9641165083305978 1102 58-59 1.001137698722367 1102 60-61 1.123017222355955 1102 62-63 1.2091697891111792 1102 64-65 1.2065408641304494 1102 66-67 1.1148600845836718 1102 68-69 1.1483485454943505 1102 70-71 1.1423780262474956 1102 72-73 1.4535088604886717 1102 74-75 1.5418213688420508 1102 76-77 1.516613035187028 1102 78-79 1.9169679884086825 1102 80-81 2.1340824690778533 1102 82-83 2.1221659770259116 1102 84-85 2.2334556855944783 1102 86-87 2.2397345077976745 1102 88-89 2.2955281780149335 1102 90-91 2.472103318691012 1102 92-93 2.7247121186137306 1102 94-95 2.808004987430259 1102 96-97 3.365825437652404 1102 98-99 3.678128888571166 1102 100-101 4.027044240395458 1103 1 0.27635633851808095 1103 2 0.32510823649664644 1103 3 0.2785176768437978 1103 4 0.2104055448470845 1103 5 0.20105704497430565 1103 6 0.1604573276292669 1103 7 0.23115871502633212 1103 8 0.29342010474776714 1103 9 0.3598148650155082 1103 10-11 0.47174704404588397 1103 12-13 0.44604705060149286 1103 14-15 0.49098652566873113 1103 16-17 0.5837305301671947 1103 18-19 0.4613851939746283 1103 20-21 0.5475111262717363 1103 22-23 0.46280636837200007 1103 24-25 0.5047098524891283 1103 26-27 0.535704067535768 1103 28-29 0.4590397854080521 1103 30-31 0.6965864871022447 1103 32-33 0.7293314049464215 1103 34-35 0.6946225645734074 1103 36-37 0.7938826411865207 1103 38-39 0.8845437720643332 1103 40-41 0.8356323347677019 1103 42-43 0.8853934286978671 1103 44-45 0.8658817908365393 1103 46-47 0.8983748913463927 1103 48-49 1.0860223547603027 1103 50-51 1.094263497820215 1103 52-53 1.318047140656347 1103 54-55 1.3884708949168996 1103 56-57 1.3086036089327848 1103 58-59 1.5520386434290216 1103 60-61 1.5827060929463173 1103 62-63 1.5244034906265327 1103 64-65 1.5407058107663296 1103 66-67 1.7794051527229477 1103 68-69 1.753449925063567 1103 70-71 1.8236623037876036 1103 72-73 1.7310182195465735 1103 74-75 1.8319933658621785 1103 76-77 1.5687138358079658 1103 78-79 2.005885476312418 1103 80-81 2.246548700881956 1103 82-83 2.477769278394394 1103 84-85 2.651142176771046 1103 86-87 2.8055484451518957 1103 88-89 2.9327136229341093 1103 90-91 3.1216876786653565 1103 92-93 3.2259374228039412 1103 94-95 3.355351959221757 1103 96-97 3.587351770710349 1103 98-99 3.866601395193019 1103 100-101 4.26742850330281 1104 1 0.3645306659257095 1104 2 0.37903378072491023 1104 3 0.3572089479696743 1104 4 0.35125045370168095 1104 5 0.29203238277609955 1104 6 0.2610026169876676 1104 7 0.1929732604349894 1104 8 0.2838492928419072 1104 9 0.31698782618852306 1104 10-11 0.35669999441290656 1104 12-13 0.40178265495095644 1104 14-15 0.47573654832459766 1104 16-17 0.5533398398344289 1104 18-19 0.5699843561091669 1104 20-21 0.6902680179560647 1104 22-23 0.5657563339006728 1104 24-25 0.4348517871737059 1104 26-27 0.5427798665994743 1104 28-29 0.5966143633493814 1104 30-31 0.5917066148010193 1104 32-33 0.6667093070478529 1104 34-35 0.666688458149352 1104 36-37 0.7582137081687321 1104 38-39 0.5750381459537621 1104 40-41 0.6617926017742306 1104 42-43 0.7168670693173596 1104 44-45 0.7954237753066593 1104 46-47 0.8364845204899822 1104 48-49 0.7909381896604302 1104 50-51 0.9000866857239345 1104 52-53 1.0045411772888713 1104 54-55 1.0681339018490235 1104 56-57 1.2465339986480544 1104 58-59 1.2500333987399515 1104 60-61 1.181572123948719 1104 62-63 1.2623089875700018 1104 64-65 1.2651323593089536 1104 66-67 1.5466316390910002 1104 68-69 1.5546329626470659 1104 70-71 1.5973330928655258 1104 72-73 1.821179666952279 1104 74-75 1.8949782443636565 1104 76-77 1.8671903580239047 1104 78-79 2.2034715657267974 1104 80-81 2.3724830401738792 1104 82-83 2.4568653725234846 1104 84-85 2.6841023001565993 1104 86-87 2.911594807652598 1104 88-89 3.139441926933358 1104 90-91 3.1242020493657634 1104 92-93 3.443452785773818 1104 94-95 3.86920200493498 1104 96-97 4.023661835993654 1104 98-99 4.460485143654427 1104 100-101 4.489165600071452 1105 1 0.26929414372697735 1105 2 0.2458149646572565 1105 3 0.26808038031639825 1105 4 0.19673472960020177 1105 5 0.050117962409643724 1105 6 0.16012611945419053 1105 7 0.23095665543146282 1105 8 0.2791948266685438 1105 9 0.23642823316526318 1105 10-11 0.35650454427197076 1105 12-13 0.36105965459859846 1105 14-15 0.45025865896589323 1105 16-17 0.4751746486786814 1105 18-19 0.3988603251014098 1105 20-21 0.4491150272936224 1105 22-23 0.38327472273083174 1105 24-25 0.29664541384742193 1105 26-27 0.4696148956692454 1105 28-29 0.4935042285713749 1105 30-31 0.43834542294055723 1105 32-33 0.5277844479921825 1105 34-35 0.5182933515602031 1105 36-37 0.7092217904449782 1105 38-39 0.6493554468699045 1105 40-41 0.46818795061143703 1105 42-43 0.5586917477881173 1105 44-45 0.596322845955001 1105 46-47 0.8135860224279625 1105 48-49 0.8220125598154695 1105 50-51 0.8962569640889768 1105 52-53 0.8750645432155792 1105 54-55 0.8352079857109018 1105 56-57 0.9423243880067602 1105 58-59 0.910272783870326 1105 60-61 0.9784724498824744 1105 62-63 1.0733461396136974 1105 64-65 1.0860752548339754 1105 66-67 1.1358796790839527 1105 68-69 1.1565169918930103 1105 70-71 1.199081683422257 1105 72-73 1.5112964965858247 1105 74-75 1.6945559619464596 1105 76-77 1.5602609623227046 1105 78-79 1.8864778641764097 1105 80-81 2.217861607827153 1105 82-83 2.3828993312972706 1105 84-85 2.373907510692188 1105 86-87 2.3681255951790234 1105 88-89 2.5130655174900873 1105 90-91 2.683914544961251 1105 92-93 3.0803592780218843 1105 94-95 3.0442840389377963 1105 96-97 3.333295600968988 1105 98-99 3.777941318777753 1105 100-101 4.399481513566869 1106 1 0.1658526880307747 1106 2 0.19407445832813863 1106 3 0.2095954119619634 1106 4 0.10835788360864029 1106 5 0.05216833160795886 1106 6 0.12660818696473797 1106 7 0.159589303110792 1106 8 0.011928212744493294 1106 9 0.19939632388256712 1106 10-11 0.26501258752091417 1106 12-13 0.04730042464079531 1106 14-15 0.15683830031610313 1106 16-17 0.13662968559851407 1106 18-19 0.27190884830815776 1106 20-21 0.439136783622736 1106 22-23 0.32379094108526374 1106 24-25 0.18252687481788854 1106 26-27 0.06512188406586716 1106 28-29 0.3005420713983753 1106 30-31 0.4177725062738844 1106 32-33 0.3960465787938716 1106 34-35 0.225766320969484 1106 36-37 0.4861699708247329 1106 38-39 0.36193456079395503 1106 40-41 0.36845166369160154 1106 42-43 0.5159405610792547 1106 44-45 0.05647909595500522 1106 46-47 0.19159564795538842 1106 48-49 0.4961192317563956 1106 50-51 0.19764145775986464 1106 52-53 0.5065683670552374 1106 54-55 0.43780555955056144 1106 56-57 0.43120228885064193 1106 58-59 0.863931802857671 1106 60-61 0.6775824182369021 1106 62-63 0.6901413664069409 1106 64-65 0.5175593001926195 1106 66-67 0.634927015581841 1106 68-69 0.5048753779689612 1106 70-71 0.8015869576838632 1106 72-73 1.280155278231394 1106 74-75 1.4099864735498358 1106 76-77 1.4368630192847291 1106 78-79 1.7007117776785172 1106 80-81 1.6382400360971943 1106 82-83 2.0130351435335534 1106 84-85 1.89981402440527 1106 86-87 2.2078605635334583 1106 88-89 2.495858239009074 1106 90-91 2.4699410481258077 1106 92-93 2.1812361240134415 1106 94-95 2.661784698220501 1106 96-97 3.0998370039225733 1106 98-99 3.425073439030921 1106 100-101 3.6849014766470383 1107 1 0.21594164119916925 1107 2 0.11605005060265228 1107 3 0.10730086818292506 1107 4 0.1327101466881686 1107 5 0.09065817558794009 1107 6 0.033017508062215484 1107 7 0.07827981434619602 1107 8 0.09244842879190429 1107 9 0.14206366036445672 1107 10-11 0.18124728736263052 1107 12-13 0.18926312190563976 1107 14-15 0.3026737668189412 1107 16-17 0.28752956315458533 1107 18-19 0.3560055896784249 1107 20-21 0.3703167356690855 1107 22-23 0.30780523545412564 1107 24-25 0.3424489927992269 1107 26-27 0.37897804657925604 1107 28-29 0.41368164894549153 1107 30-31 0.39289468355733703 1107 32-33 0.3601898659227558 1107 34-35 0.48797258352852424 1107 36-37 0.41741951752620565 1107 38-39 0.3643685072002043 1107 40-41 0.49306156031821047 1107 42-43 0.618121780228293 1107 44-45 0.6573596149978087 1107 46-47 0.7816311332301211 1107 48-49 0.7604781289930926 1107 50-51 0.6997695609882015 1107 52-53 0.8876990962152433 1107 54-55 0.8778131003047633 1107 56-57 0.8867353039150814 1107 58-59 0.9914158787473113 1107 60-61 1.0918924658919096 1107 62-63 1.152303472357211 1107 64-65 1.2832980914534495 1107 66-67 1.4224014708770092 1107 68-69 1.3626116371575883 1107 70-71 1.308129121912316 1107 72-73 1.4393201210381328 1107 74-75 1.4959870687041281 1107 76-77 1.553684980691429 1107 78-79 1.8526121117328636 1107 80-81 2.045419431386307 1107 82-83 1.9303004308928173 1107 84-85 2.316318036897112 1107 86-87 2.5360777564530395 1107 88-89 2.6706373493067375 1107 90-91 2.627338826643083 1107 92-93 2.940545809029633 1107 94-95 2.969222686982963 1107 96-97 3.420251596081897 1107 98-99 3.8264999428087627 1107 100-101 4.14082246191537 1108 1 0.24919134352515826 1108 2 0.2156308072405011 1108 3 0.11594081169883452 1108 4 0.10311874826821565 1108 5 0.06544286570597535 1108 6 0.13094894151944203 1108 7 0.061207559400521916 1108 8 0.15915036400457439 1108 9 0.2196349057182445 1108 10-11 0.216748037797025 1108 12-13 0.26254857572204315 1108 14-15 0.35164509522556386 1108 16-17 0.4229890932632756 1108 18-19 0.3265932548020487 1108 20-21 0.351716828924971 1108 22-23 0.2897756792562305 1108 24-25 0.30105725584203924 1108 26-27 0.23090320924139007 1108 28-29 0.2963146788740971 1108 30-31 0.46858397616961867 1108 32-33 0.5123339350422995 1108 34-35 0.49060956203783235 1108 36-37 0.53400289328723 1108 38-39 0.5022586591134299 1108 40-41 0.510468475333937 1108 42-43 0.5630957063316728 1108 44-45 0.7628152883532522 1108 46-47 0.9380439037767658 1108 48-49 0.8866865528359895 1108 50-51 0.9159477225820822 1108 52-53 0.9145966341170251 1108 54-55 0.8998407220042068 1108 56-57 0.9856607696078399 1108 58-59 0.9934269000829019 1108 60-61 0.9699420302726551 1108 62-63 1.1891905623348507 1108 64-65 1.114795980952369 1108 66-67 1.2389529110886564 1108 68-69 1.2505670885966786 1108 70-71 1.2773128472298616 1108 72-73 1.4124508966026426 1108 74-75 1.534610462871452 1108 76-77 1.6247512709335368 1108 78-79 1.823182686746204 1108 80-81 1.9937965851228157 1108 82-83 2.127628455918863 1108 84-85 2.1412224598176515 1108 86-87 2.2417901385695416 1108 88-89 2.483523598552985 1108 90-91 2.496162537309047 1108 92-93 2.758834828812329 1108 94-95 3.0554167857423735 1108 96-97 3.2829366411640777 1108 98-99 3.575185789850746 1108 100-101 3.960201167535928 1109 1 0.3455967895153158 1109 2 0.3184661525622232 1109 3 0.2588032474870232 1109 4 0.2153126183690972 1109 5 0.14344837097696228 1109 6 0.20383112305390227 1109 7 0.2431675535596156 1109 8 0.28256618196012084 1109 9 0.2551816078952882 1109 10-11 0.315605628685212 1109 12-13 0.36802622227318693 1109 14-15 0.349554465301388 1109 16-17 0.3197137730487327 1109 18-19 0.47684808608052975 1109 20-21 0.42782104331234194 1109 22-23 0.41870657568260583 1109 24-25 0.41815650546009664 1109 26-27 0.3799263832502362 1109 28-29 0.6816741124806569 1109 30-31 0.5186994321198242 1109 32-33 0.611776978589738 1109 34-35 0.695783339861137 1109 36-37 0.6915016455565777 1109 38-39 0.6686904000736504 1109 40-41 0.7647862227496631 1109 42-43 0.9517842378889085 1109 44-45 0.9659913349758824 1109 46-47 0.9048392846669842 1109 48-49 0.8564173699306608 1109 50-51 0.65811126574485 1109 52-53 0.8193708112141422 1109 54-55 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2316 6 -0.43777152133728237 2316 7 -0.43416812259999205 2316 8 -0.3642261842396195 2316 9 -0.47173941650495266 2316 10-11 -0.4549827242465483 2316 12-13 -0.4389411381462054 2316 14-15 -0.6757544688879875 2316 16-17 -0.3914234455628218 2316 18-19 -0.5472076606926066 2316 20-21 -0.6348132610118071 2316 22-23 -0.6779073390398338 2316 24-25 -0.7489679328040282 2316 26-27 -0.608669049663078 2316 28-29 -0.5770782010328901 2316 30-31 -0.6146031913415371 2316 32-33 -0.6596410339966567 2316 34-35 -0.4320661582064105 2316 36-37 -0.46410987812426896 2316 38-39 -0.46022408128839487 2316 40-41 -0.4669211310729864 2316 42-43 -0.3551872317907794 2316 44-45 -0.4054374438725006 2316 46-47 -0.6240095033203872 2316 48-49 -0.4880652084493775 2316 50-51 -0.22847191718956594 2316 52-53 -0.3334673726900519 2316 54-55 -0.14744200160518517 2316 56-57 0.06469168252730384 2316 58-59 0.047110841962670236 2316 60-61 0.4244571974725275 2316 62-63 0.2767616805827444 2316 64-65 0.34104774658942816 2316 66-67 0.5814153367450352 2316 68-69 0.7098980484632804 2316 70-71 0.6656310238585839 2316 72-73 1.0037269728668967 2316 74-75 0.9518012530423512 2316 76-77 0.9293859306129129 2316 78-79 1.0276252829993417 2316 80-81 1.1570652164014845 2316 82-83 1.237919784113096 2316 84-85 1.4387528461817887 2316 86-87 1.4867305228806984 2316 88-89 1.75891946218821 2316 90-91 1.7601952241849936 2316 92-93 1.8320422752314194 2316 94-95 2.413368180870826 2316 96-97 2.6845346610907512 2316 98-99 3.1200352748914035 2316 100-101 3.719772213084884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 7.0 4 30.0 5 50.0 6 149.0 7 405.0 8 834.0 9 1515.0 10 2541.0 11 3947.0 12 5752.0 13 7776.0 14 10343.0 15 13741.0 16 17031.0 17 21225.0 18 20722.0 19 18073.0 20 18325.0 21 19673.0 22 21307.0 23 23474.0 24 27159.0 25 31738.0 26 37484.0 27 43901.0 28 52081.0 29 62272.0 30 72886.0 31 86006.0 32 102710.0 33 123694.0 34 153537.0 35 195459.0 36 247011.0 37 277243.0 38 152915.0 39 9461.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.03260160511324 19.214665303928214 15.452986585808965 23.299746505149578 2 15.994555795654202 20.264727359305795 39.42658349073199 24.314133354308012 3 19.370872800309805 23.65314709683657 29.962374122235495 27.013605980618134 4 13.456521565908892 17.108237250230413 36.30780590549194 33.12743527836875 5 13.915831275431309 37.07020689625834 34.24779692814253 14.766164900167823 6 32.69970278647639 36.21925274304974 16.804967901470665 14.276076569003207 7 28.990934854726596 32.419169342650804 20.08074433528023 18.50915146734237 8 26.466452587935628 35.39077336605604 19.214814460672994 18.92795958533534 9 26.12360789063392 16.81426412428253 19.821884370924604 37.24024361415895 10-11 25.160970738146656 26.497501972127267 27.050228819884353 21.291298469841724 12-13 26.071363118431222 23.648395604745854 27.471932047267313 22.808309229555615 14-15 22.509927037931245 25.31390688372019 25.73653973338433 26.439626344964235 16-17 22.277468346361324 28.394701684209966 26.718698953776528 22.609131015652185 18-19 22.35074914275545 26.771793666350597 29.064587500033202 21.81286969086075 20-21 22.63683375963155 25.98830800776633 29.374736053673733 22.000122178928386 22-23 22.482250854588486 25.939808285324983 29.25975505780923 22.31818580227731 24-25 22.455250140241727 26.14974310265694 29.199150687609432 22.1958560694919 26-27 21.94758611749599 26.57397177926529 28.996235127985194 22.48220697525353 28-29 22.099140232193086 26.81006754369889 28.97114718045562 22.119645043652405 30-31 22.25614015516724 26.34645163175271 28.85127902745573 22.546129185624324 32-33 21.923313211778854 26.399360841615206 28.85230113220597 22.825024814399974 34-35 22.668747551086817 26.58265206505642 28.594300590395612 22.154299793461153 36-37 22.77437111637501 26.10502527859041 28.647094728443072 22.4735088765915 38-39 22.23683228366683 26.481905173263375 28.611972440642642 22.669290102427155 40-41 22.765341537232313 26.2591793161446 28.639944495119686 22.33553465150341 42-43 22.40202743712536 26.328207986021923 28.454934493710475 22.81483008314224 44-45 22.395423153511828 26.620145274874545 28.609035707275403 22.375395864338223 46-47 22.784774152010932 26.875382141565158 27.815231266364687 22.52461244005922 48-49 22.504508668063757 26.911954145716965 27.817698414595597 22.765838771623677 50-51 22.81107153576257 27.045740072298052 27.700884734805324 22.442303657134055 52-53 23.040635117942507 26.793626509640184 27.68978238870429 22.475955983713018 54-55 22.542516939045996 26.956283848211278 27.88372815719647 22.61747105554626 56-57 22.63125602594443 27.039896732244877 27.68279694127709 22.646050300533606 58-59 22.603181433052587 26.70698571303357 27.646701036130434 23.043131817783408 60-61 22.331200514213926 26.969643848423758 27.94046167698547 22.758693960376842 62-63 22.424401788062056 27.0335487400962 27.972600047277936 22.56944942456381 64-65 22.13220822476673 27.003800827098225 27.734377697564653 23.129613250570387 66-67 22.126695137981585 27.00823568065416 27.74024896419855 23.124820217165713 68-69 22.51973987561116 26.769470550612912 27.81829752603475 22.892492047741175 70-71 22.32108091166729 26.86526054656478 27.528426521326864 23.285232020441065 72-73 22.391068189122358 26.896602097759082 27.472702305728863 23.2396274073897 74-75 22.330111528113108 27.235834548482458 27.310841786255935 23.123212137148503 76-77 22.394255465515 26.85139589425679 27.595678053211582 23.15867058701663 78-79 22.496699840901787 27.052380231449387 27.528665567056308 22.922254360592515 80-81 22.604515468786065 27.169254248839763 27.240383652561285 22.98584662981289 82-83 22.51250355252289 27.22047953082793 27.407069985364934 22.85994693128425 84-85 22.515746424201087 27.20449274773343 27.323489045832964 22.956271782232523 86-87 22.645950135408068 27.161429365223555 27.11715214894569 23.075468350422693 88-89 22.465762666306148 27.353213266829023 26.867923746516905 23.313100320347925 90-91 22.403024963492093 27.29468586202482 27.07261049185936 23.229678682623724 92-93 22.511087773090697 26.958441501870674 27.213610351031804 23.31686037400683 94-95 22.215157092885203 26.87243191844822 27.271346119623797 23.64106486904278 96-97 22.453804412784166 26.583983404914246 27.294267949014202 23.667944233287383 98-99 22.158343891186384 26.797185634945297 26.9917422980794 24.052728175788918 100-101 22.350593821199713 26.507209414869514 27.249691761192352 23.892505002738424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 361.0 1 249.0 2 210.5 3 275.0 4 230.0 5 229.5 6 418.0 7 957.0 8 1551.0 9 1767.5 10 1548.5 11 1291.0 12 1309.0 13 1473.5 14 1702.0 15 1892.0 16 1998.0 17 2154.0 18 2396.5 19 2660.0 20 3032.0 21 3550.0 22 4109.5 23 4989.5 24 6255.0 25 7921.5 26 10183.5 27 12801.5 28 15255.5 29 17994.0 30 21124.5 31 25441.5 32 31190.0 33 33873.0 34 34518.5 35 37061.5 36 41107.0 37 45310.0 38 48042.5 39 50206.0 40 53159.0 41 57116.0 42 60244.5 43 62532.5 44 65398.5 45 68490.0 46 69143.0 47 70048.0 48 71161.5 49 70035.0 50 69464.0 51 67943.0 52 66021.5 53 66094.5 54 66458.5 55 62238.5 56 56603.5 57 51863.0 58 47277.0 59 41931.5 60 34211.0 61 28298.5 62 24139.0 63 18385.0 64 13133.5 65 10134.5 66 7951.5 67 6430.5 68 5058.5 69 3614.5 70 2520.0 71 1775.0 72 1314.5 73 1078.5 74 839.0 75 618.0 76 464.0 77 318.0 78 200.0 79 129.5 80 77.0 81 43.0 82 21.5 83 15.5 84 14.5 85 11.0 86 4.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.3121273210676313E-5 2 0.04520620350228554 3 2.1248509284270525E-4 4 0.0 5 0.10247093602339462 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011155467374242027 26-27 0.011500755650111423 28-29 0.0 30-31 0.0 32-33 5.04652095501425E-4 34-35 0.014475546949909294 36-37 0.003293518939061931 38-39 0.011713240742954127 40-41 0.006799522970966568 42-43 0.00576365814335838 44-45 0.0028419881167711827 46-47 1.0624254642135263E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.9216700265871973E-4 68-69 5.3121273210676313E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.6560636605338157E-5 82-83 0.0010624254642135262 84-85 0.0039044135809847094 86-87 0.0014342743766882606 88-89 2.6560636605338157E-5 90-91 8.49940371370821E-4 92-93 3.1872763926405785E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.49940371370821E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1882485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.600793864434934 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4363280720191 47.34468214768036 2 12.12254182300289 14.450262326115732 3 3.6352624788330274 6.499935889321263 4 1.601983731944701 3.8191800872725725 5 0.8615532089758867 2.567462760570713 6 0.5043900149164986 1.8037227183790496 7 0.34410053102154875 1.4356065372640547 8 0.2433463345322034 1.1602907777695743 9 0.1690141572757265 0.9066040153165583 >10 0.9866375479742199 10.51084487854505 >50 0.05820069766444483 2.3909075210554094 >100 0.03145919737761023 3.473685881627801 >500 0.0029484956422140655 1.2237384875640678 >1k 0.0020550121142704084 1.8427967955680833 >5k 1.7869670558873116E-4 0.5702791759496778 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5683 0.3018881956562735 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5026 0.26698751915685914 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3310 0.1758314143273386 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2142 0.11378576721726866 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2040 0.10836739734977968 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2013 0.10693312297309142 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1983 0.10533948477677113 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1902 0.10103666164670635 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.3121273210676313E-5 2 0.0 0.0 0.0 0.0 5.3121273210676313E-5 3 0.0 0.0 0.0 0.0 5.3121273210676313E-5 4 0.0 0.0 0.0 5.3121273210676313E-5 5.3121273210676313E-5 5 0.0 0.0 0.0 5.3121273210676313E-5 5.3121273210676313E-5 6 0.0 0.0 0.0 5.3121273210676313E-5 5.3121273210676313E-5 7 0.0 0.0 0.0 5.3121273210676313E-5 5.3121273210676313E-5 8 0.0 0.0 0.0 5.3121273210676313E-5 5.3121273210676313E-5 9 0.0 0.0 0.0 5.3121273210676313E-5 5.3121273210676313E-5 10-11 0.0 0.0 0.0 1.0624254642135263E-4 5.3121273210676313E-5 12-13 0.0 0.0 0.0 1.3280318302669078E-4 3.187276392640579E-4 14-15 0.0 0.0 0.0 1.859244562373671E-4 3.187276392640579E-4 16-17 0.0 0.0 0.0 2.1248509284270525E-4 3.187276392640579E-4 18-19 0.0 0.0 0.0 2.1248509284270525E-4 3.718489124747342E-4 20-21 0.0 0.0 0.0 3.4528827586939603E-4 4.249701856854105E-4 22-23 0.0 0.0 0.0 4.5153082229074863E-4 4.249701856854105E-4 24-25 0.0 0.0 0.0 7.436978249494684E-4 4.249701856854105E-4 26-27 0.0 0.0 0.0 0.0012217892838455552 4.780914588960868E-4 28-29 0.0 0.0 0.0 0.003612246578325989 4.780914588960868E-4 30-31 0.0 0.0 0.0 0.010650815278740601 4.780914588960868E-4 32-33 0.0 0.0 0.0 0.02539196859470328 4.780914588960868E-4 34-35 0.0 5.3121273210676313E-5 0.0 0.04815443416547808 5.312127321067631E-4 36-37 0.0 1.0624254642135263E-4 0.0 0.08602990196469029 5.312127321067631E-4 38-39 0.0 1.0624254642135263E-4 0.0 0.14448986313303958 5.843340053174395E-4 40-41 0.0 1.0624254642135263E-4 0.0 0.2087666037179579 5.843340053174395E-4 42-43 0.0 1.0624254642135263E-4 0.0 0.2755134835071727 5.843340053174395E-4 44-45 0.0 1.3280318302669078E-4 0.0 0.35817018462298505 5.843340053174395E-4 46-47 0.0 1.5936381963202895E-4 0.0 0.4574272836171337 6.640159151334539E-4 48-49 0.0 1.5936381963202895E-4 0.0 0.5631651779429849 7.968190981601447E-4 50-51 0.0 1.5936381963202895E-4 0.0 0.6768712632504376 7.968190981601447E-4 52-53 0.0 1.5936381963202895E-4 0.0 0.7877884817143297 8.49940371370821E-4 54-55 0.0 1.5936381963202895E-4 0.0 0.9130484439451045 8.765010079761592E-4 56-57 0.0 1.5936381963202895E-4 0.0 1.0608583866538113 9.030616445814974E-4 58-59 0.0 1.5936381963202895E-4 0.0 1.2249234389649852 9.030616445814974E-4 60-61 0.0 1.5936381963202895E-4 0.0 1.4030390680403828 9.296222811868355E-4 62-63 0.0 1.859244562373671E-4 0.0 1.5745145379644458 0.0010624254642135262 64-65 0.0 2.1248509284270525E-4 0.0 1.7529223340425024 0.0010624254642135262 66-67 0.0 2.1248509284270525E-4 0.0 1.9624060749488041 0.0010624254642135262 68-69 0.0 2.1248509284270525E-4 0.0 2.201478365033453 0.0010624254642135262 70-71 0.0 2.1248509284270525E-4 0.0 2.456487037081305 0.0011155467374242027 72-73 0.0 2.1248509284270525E-4 0.0 2.720898174487446 0.001168668010634879 74-75 0.0 2.1248509284270525E-4 0.0 2.986876389453302 0.0012749105570562316 76-77 0.0 2.1248509284270525E-4 0.0 3.2768654199103846 0.0013280318302669079 78-79 0.0 2.1248509284270525E-4 0.0 3.6086343317476635 0.0013811531034775841 80-81 0.0 2.6560636605338155E-4 0.0 3.9648390292618534 0.0013811531034775841 82-83 0.0 2.6560636605338155E-4 0.0 4.328401023115722 0.0013811531034775841 84-85 0.0 2.6560636605338155E-4 0.0 4.693237927526647 0.0013811531034775841 86-87 0.0 2.6560636605338155E-4 0.0 5.077278172203231 0.0014342743766882606 88-89 0.0 2.6560636605338155E-4 0.0 5.491039769241189 0.001540516923109613 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3090 0.0 54.49087 1 GTATCAA 5495 0.0 43.279404 1 ATCAACG 7475 0.0 31.549156 3 TATCAAC 7525 0.0 31.477615 2 TCAACGC 7805 0.0 30.276157 4 CAACGCA 7840 0.0 30.080349 5 AACGCAG 8220 0.0 28.71631 6 ACGCAGA 9375 0.0 25.026478 7 CGCAGAG 9375 0.0 25.026478 8 TGGTATC 1425 0.0 23.698587 2 GTGGTAT 1540 0.0 23.473175 1 TAAGGTG 1160 0.0 22.543533 5 GTAAGGT 1135 0.0 22.202265 4 GTACATG 6315 0.0 22.068523 1 GCAGAGT 10505 0.0 21.882322 9 TACATGG 6260 0.0 21.274662 2 GTACCGT 180 5.1767565E-6 21.108702 6 AGAGTAC 9600 0.0 20.75414 10-11 ACATGGG 6235 0.0 20.589443 3 GAGTACT 6650 0.0 20.39053 12-13 >>END_MODULE