FastQCFastQC Report
Thu 26 May 2016
SRR938608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884178
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55620.2951950399590697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46760.24817188184980402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29500.1565669485579388No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21930.11639027735171517No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20020.10625323085186218No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19660.10434258334403651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.053.0348931
GTATCAA57250.042.2438241
TATCAAC73900.032.3566252
ATCAACG73950.032.207033
TCAACGC76100.031.297114
CAACGCA76200.031.0680545
AACGCAG80050.029.6823526
ACGCAGA92000.025.6148177
CGCAGAG93400.025.0782628
TGGTATC15200.024.3944282
GTGGTAT16400.023.2689531
GTACATG60500.023.1804921
GCAGAGT101450.022.8973399
TACATGG61700.022.651752
GTGTAGC10750.021.7429451
ACATGGG63000.021.2794193
GAGTACT64900.021.11573812-13
GTGTAAG8950.020.7860661
AGAGTAC92800.020.70447210-11
CAGAGTA97150.020.19310-11
Produced by FastQC (version 0.11.5)