FastQCFastQC Report
Thu 26 May 2016
SRR938608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884178
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54060.28691556742515834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50150.26616381254849597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.1720113492461965No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20400.10827002544345599No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20160.10699626043823886No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20040.10635937793563029No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19930.10577556897490577No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA19360.1027503770875151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.054.4588051
GTATCAA58300.042.9575651
ATCAACG75500.032.9657443
TATCAAC76350.032.6678852
TGGTATC14750.032.5312042
TCAACGC77450.032.1348954
GTGGTAT15700.031.7824421
CAACGCA79000.031.5645245
AACGCAG81800.030.3679456
ACGCAGA92400.026.781377
CGCAGAG94000.026.3255168
GGACCGA5500.025.0439226
GTACATG64300.023.9459271
CGTACAC2002.0565494E-823.7491783
TAAGGTG12150.023.4553575
GCAGAGT104300.023.4070059
TACATGG65000.023.0233212
ACATGGG66150.022.1874393
GAGTACT65700.021.68817112-13
ACCGAGT7050.021.5589668
Produced by FastQC (version 0.11.5)