##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938608_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884178 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.810416531771413 31.0 31.0 34.0 30.0 34.0 2 32.064491252949566 33.0 31.0 34.0 30.0 34.0 3 32.118807246449116 33.0 31.0 34.0 30.0 34.0 4 35.773789419046395 37.0 35.0 37.0 35.0 37.0 5 35.66988044653955 37.0 35.0 37.0 33.0 37.0 6 35.70152766882959 37.0 35.0 37.0 33.0 37.0 7 35.628310064123454 37.0 35.0 37.0 33.0 37.0 8 35.59574732323591 37.0 35.0 37.0 33.0 37.0 9 37.141313612620465 39.0 37.0 39.0 33.0 39.0 10-11 37.06687850086351 39.0 37.0 39.0 33.0 39.0 12-13 36.9490491344236 39.0 37.0 39.0 33.0 39.0 14-15 37.9614043896065 40.0 37.0 41.0 33.0 41.0 16-17 38.0449169345996 40.0 37.0 41.0 33.0 41.0 18-19 38.185989858707615 40.0 37.5 41.0 33.0 41.0 20-21 38.13628462915924 40.0 37.0 41.0 33.0 41.0 22-23 38.01546563010501 40.0 37.0 41.0 33.0 41.0 24-25 37.895119781676684 40.0 37.0 41.0 32.5 41.0 26-27 37.67204690851926 40.0 37.0 41.0 32.0 41.0 28-29 37.52988438459636 39.5 36.5 41.0 32.0 41.0 30-31 37.25889300267809 39.0 36.0 41.0 31.0 41.0 32-33 37.134441119681895 39.0 36.0 41.0 31.0 41.0 34-35 36.993507513621324 39.0 36.0 41.0 31.0 41.0 36-37 36.851325883223346 39.0 35.5 41.0 30.0 41.0 38-39 36.62693015203447 39.0 35.0 40.0 30.0 41.0 40-41 36.393262207710734 39.0 35.0 40.0 30.0 41.0 42-43 36.12122713459132 38.5 35.0 40.0 29.5 41.0 44-45 35.8092836770199 38.0 35.0 40.0 28.5 41.0 46-47 35.386574676065635 38.0 34.0 40.0 27.0 41.0 48-49 34.95874593589353 38.0 34.0 40.0 26.0 41.0 50-51 34.549834198255155 38.0 33.0 40.0 24.5 41.0 52-53 33.93373794832547 37.5 33.0 40.0 23.5 41.0 54-55 33.48471720824678 37.0 32.0 40.0 22.5 41.0 56-57 33.08262701294676 36.5 32.0 39.0 21.0 40.5 58-59 32.534344950424 36.0 31.0 39.0 19.0 40.0 60-61 32.03000618837498 35.5 31.0 39.0 17.5 40.0 62-63 31.50234425834502 35.0 30.0 38.5 12.5 40.0 64-65 30.83840831386419 34.5 29.0 38.0 8.0 40.0 66-67 30.179143371804578 34.0 28.0 37.5 7.0 40.0 68-69 29.441003185473985 34.0 27.5 37.0 2.0 39.0 70-71 28.651685244175447 33.5 26.0 36.0 2.0 39.0 72-73 28.062564948746882 33.0 26.0 36.0 2.0 38.5 74-75 27.347826213871514 32.0 25.0 35.0 2.0 38.0 76-77 26.219975235885357 30.5 23.0 34.0 2.0 36.0 78-79 26.69747099265568 32.0 25.0 35.0 2.0 36.0 80-81 26.321243268948052 32.0 23.5 35.0 2.0 36.0 82-83 25.63910814158747 31.5 21.0 34.5 2.0 36.0 84-85 24.84994172525101 31.0 19.0 34.0 2.0 35.0 86-87 23.935069563491346 30.5 11.5 34.0 2.0 35.0 88-89 22.961979441432817 29.5 4.5 34.0 2.0 35.0 90-91 22.100717660433354 29.0 2.0 34.0 2.0 35.0 92-93 21.016617591331602 28.5 2.0 33.5 2.0 35.0 94-95 19.855024047621825 26.5 2.0 33.0 2.0 34.5 96-97 18.48542627076635 25.0 2.0 32.5 2.0 34.0 98-99 16.745718557376215 11.5 2.0 32.0 2.0 34.0 100-101 12.57576646155512 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3028253887613914 1101 2 0.25673354957674377 1101 3 0.26470879936786673 1101 4 0.19953658028133958 1101 5 0.22584113189775223 1101 6 0.2641153470722344 1101 7 0.23736082577113393 1101 8 0.2969639371536985 1101 9 0.33632118378900344 1101 10-11 0.2424467030671167 1101 12-13 0.32769391312901064 1101 14-15 0.4293651317051683 1101 16-17 0.334488793429621 1101 18-19 0.4151921677358317 1101 20-21 0.38425190488081995 1101 22-23 0.3515036267490004 1101 24-25 0.2699958452898059 1101 26-27 0.3948675690026491 1101 28-29 0.37273632597774764 1101 30-31 0.5863033928379053 1101 32-33 0.4667847645566283 1101 34-35 0.30020247711667025 1101 36-37 0.28614325178973843 1101 38-39 0.6240361109514865 1101 40-41 0.6812562229095676 1101 42-43 0.6668892107838076 1101 44-45 0.5947751782425215 1101 46-47 0.6717771170712012 1101 48-49 0.8705454848053833 1101 50-51 0.8431615694894461 1101 52-53 1.0812820221970014 1101 54-55 1.0081393394707305 1101 56-57 0.823585065308059 1101 58-59 0.9628070542050011 1101 60-61 0.7745841876644946 1101 62-63 0.9628711149556501 1101 64-65 0.9686760373859933 1101 66-67 1.0756926702909588 1101 68-69 1.4240869000971692 1101 70-71 1.4807146179455621 1101 72-73 1.6934945866351931 1101 74-75 1.7520721512851942 1101 76-77 1.7316748513507179 1101 78-79 1.8885749862137438 1101 80-81 2.030247883410759 1101 82-83 2.085146369653561 1101 84-85 2.1218532737148017 1101 86-87 2.3653100000537854 1101 88-89 2.3128065216713267 1101 90-91 2.5219278164513526 1101 92-93 2.799200965091817 1101 94-95 2.548597525818348 1101 96-97 2.40953523976858 1101 98-99 2.4304111291249875 1101 100-101 2.016537641360115 1102 1 0.23602109986637743 1102 2 0.2177524350437281 1102 3 0.22798461827868266 1102 4 0.1919495824764823 1102 5 0.19663211570743044 1102 6 0.276034590574497 1102 7 0.20777758837184734 1102 8 0.2643850479978269 1102 9 0.21571028413361404 1102 10-11 0.286599473151 1102 12-13 0.33790422356636185 1102 14-15 0.4386014632206141 1102 16-17 0.34932119063486056 1102 18-19 0.41460291833009677 1102 20-21 0.314525836060497 1102 22-23 0.33974169417405875 1102 24-25 0.39321688298357316 1102 26-27 0.46561874671726144 1102 28-29 0.4577102622390612 1102 30-31 0.39682026353303 1102 32-33 0.352044401301697 1102 34-35 0.5769969352550248 1102 36-37 0.43184736169847326 1102 38-39 0.3904866306264907 1102 40-41 0.4988263635075896 1102 42-43 0.6522360821627515 1102 44-45 0.6200725947046166 1102 46-47 0.45542252098857716 1102 48-49 0.7204418446650251 1102 50-51 0.7380490114035965 1102 52-53 0.6951754472589613 1102 54-55 0.47319561067162397 1102 56-57 0.6376107695685391 1102 58-59 0.8568005058043653 1102 60-61 0.8579541170925822 1102 62-63 1.0340997148664464 1102 64-65 1.1182492432729028 1102 66-67 1.0206631748841843 1102 68-69 1.3944482980345292 1102 70-71 1.569328349511327 1102 72-73 1.5022532763778962 1102 74-75 1.4700790610461283 1102 76-77 1.5770096648392418 1102 78-79 1.549105690232686 1102 80-81 1.8301468154530696 1102 82-83 1.8828023270481786 1102 84-85 2.091994938243211 1102 86-87 2.047704256683584 1102 88-89 2.0010109311452418 1102 90-91 2.2440295155348373 1102 92-93 2.504849007692183 1102 94-95 2.2905461235710156 1102 96-97 2.2550922961125544 1102 98-99 2.443655674058821 1102 100-101 2.4290468871020536 1103 1 0.25414729690865556 1103 2 0.28755091281600187 1103 3 0.3231273312890366 1103 4 0.22217262082510558 1103 5 0.2600547404381359 1103 6 0.22158064763186047 1103 7 0.3841223232318072 1103 8 0.2491467319928944 1103 9 0.37135112409150395 1103 10-11 0.3968429624061045 1103 12-13 0.3189205591742734 1103 14-15 0.5532192918233747 1103 16-17 0.43834633483977115 1103 18-19 0.40502128432142825 1103 20-21 0.41992834942183066 1103 22-23 0.475638391373856 1103 24-25 0.4959244341131068 1103 26-27 0.4716697396211771 1103 28-29 0.5232440995923184 1103 30-31 0.4529768851570637 1103 32-33 0.4988008385104834 1103 34-35 0.4497416387637827 1103 36-37 0.6774829330816132 1103 38-39 0.7101629264885858 1103 40-41 0.7801425327665257 1103 42-43 0.7466414670637604 1103 44-45 0.7010615736693566 1103 46-47 0.8947814071616378 1103 48-49 0.929368825729334 1103 50-51 1.0511433715471483 1103 52-53 1.196536470518467 1103 54-55 1.147585283659879 1103 56-57 1.3766010068178147 1103 58-59 1.3406499486397294 1103 60-61 1.2029201770006068 1103 62-63 1.4170703255399495 1103 64-65 1.5564680171324277 1103 66-67 1.505303357858974 1103 68-69 1.428274605744111 1103 70-71 1.677133042446627 1103 72-73 1.5249028520019152 1103 74-75 1.4182003950214153 1103 76-77 1.5792800543899723 1103 78-79 1.7575043007330962 1103 80-81 1.8414875663505583 1103 82-83 1.9848683285812392 1103 84-85 2.2092166411397756 1103 86-87 2.061806243096651 1103 88-89 1.9866818301226665 1103 90-91 2.539194682781069 1103 92-93 2.4503042794292895 1103 94-95 2.5496067254958454 1103 96-97 2.703637143606837 1103 98-99 2.5835522211602147 1103 100-101 2.247714709364976 1104 1 0.16269798710418115 1104 2 0.19112087204055683 1104 3 0.2616535494059846 1104 4 0.20032975036584588 1104 5 0.16327546359529777 1104 6 0.10623535679629725 1104 7 0.15382044414301532 1104 8 0.18449035898832733 1104 9 0.1932909856407008 1104 10-11 0.3074063028973626 1104 12-13 0.24780005370758573 1104 14-15 0.3651781001390546 1104 16-17 0.3157225952483529 1104 18-19 0.38363738955562354 1104 20-21 0.42030612063131656 1104 22-23 0.36429426692996714 1104 24-25 0.3190590805172704 1104 26-27 0.5316358438474182 1104 28-29 0.3675261256044422 1104 30-31 0.2244561100119924 1104 32-33 0.4412302665611847 1104 34-35 0.3437712068496239 1104 36-37 0.3348232931581947 1104 38-39 0.4867847234188858 1104 40-41 0.5076694047783192 1104 42-43 0.4123691713931308 1104 44-45 0.4051065791251034 1104 46-47 0.4102957225751851 1104 48-49 0.49634123011744435 1104 50-51 0.43815244288301614 1104 52-53 0.6759288451902776 1104 54-55 0.6842601815083214 1104 56-57 0.7509667387527301 1104 58-59 0.8292722350683803 1104 60-61 0.7282434829998223 1104 62-63 0.7463985174369405 1104 64-65 0.8929241739513429 1104 66-67 0.8618838881469664 1104 68-69 0.808130584197098 1104 70-71 1.1184305765276648 1104 72-73 1.3323704277045145 1104 74-75 1.2853667531962216 1104 76-77 1.3708741409999483 1104 78-79 1.5647339416190427 1104 80-81 1.8108199547589834 1104 82-83 1.8212607427195628 1104 84-85 2.0617351016731185 1104 86-87 2.053374747454864 1104 88-89 1.788261512874353 1104 90-91 2.2327029805448504 1104 92-93 2.3063240444871163 1104 94-95 2.072973599688236 1104 96-97 1.9369954061488812 1104 98-99 1.645757837160108 1104 100-101 1.627392946534881 1105 1 0.11360057560536418 1105 2 0.12888833390968557 1105 3 0.09784890532088042 1105 4 0.10736136905391191 1105 5 0.025085359804840834 1105 6 0.04192973837148628 1105 7 0.1321594437352971 1105 8 0.21711789796994196 1105 9 0.1905662053085564 1105 10-11 0.21823174680781676 1105 12-13 0.17955694712092907 1105 14-15 0.29670705905627415 1105 16-17 0.18311929437160757 1105 18-19 0.24802920574411047 1105 20-21 0.1528322127104289 1105 22-23 0.28640929038153473 1105 24-25 0.13966138939739636 1105 26-27 0.21052951079980886 1105 28-29 0.23015867272428636 1105 30-31 0.35331775377284913 1105 32-33 0.3624784968584933 1105 34-35 0.4329413487147775 1105 36-37 0.4031013122469034 1105 38-39 0.3896035668599893 1105 40-41 0.523784534171277 1105 42-43 0.424963354890032 1105 44-45 0.4838948665023537 1105 46-47 0.4389231716671418 1105 48-49 0.47713453847428866 1105 50-51 0.38566009153001346 1105 52-53 0.5253018648086965 1105 54-55 0.7753196114152558 1105 56-57 0.9290915280738332 1105 58-59 0.8015625433578109 1105 60-61 0.8509593225210992 1105 62-63 1.045928121633498 1105 64-65 1.265349075955566 1105 66-67 1.2456352886599795 1105 68-69 1.4913064501074054 1105 70-71 1.6058825417403675 1105 72-73 1.520286368030618 1105 74-75 1.5265021880214036 1105 76-77 1.5975060396300975 1105 78-79 1.7493287800263246 1105 80-81 2.0163570775835744 1105 82-83 1.987398906338008 1105 84-85 2.2333083794032795 1105 86-87 2.1861128828222682 1105 88-89 2.0620120465942087 1105 90-91 2.251977089433332 1105 92-93 2.405434618400495 1105 94-95 2.092321546512082 1105 96-97 2.4262672356924035 1105 98-99 2.1537086578848097 1105 100-101 1.8842430202122973 1106 1 -0.009767612585140029 1106 2 0.031153256547341357 1106 3 -0.010275105945851237 1106 4 0.046201383973333066 1106 5 -0.01571475852161086 1106 6 -0.006735230567819883 1106 7 -0.01632739244687542 1106 8 -0.04174372438860985 1106 9 0.02244296573607585 1106 10-11 0.060111594012354885 1106 12-13 -0.02049192684854262 1106 14-15 0.08110854585387273 1106 16-17 -0.07834197300758916 1106 18-19 0.05303814453589695 1106 20-21 0.05327883076036244 1106 22-23 0.016891856522164517 1106 24-25 -0.002921985736030308 1106 26-27 -0.2151466862909075 1106 28-29 0.02172566221637595 1106 30-31 0.14424809550478557 1106 32-33 0.023656488138350085 1106 34-35 -0.1588114904770066 1106 36-37 0.04844831743012179 1106 38-39 0.17878634264422288 1106 40-41 0.10814036239213465 1106 42-43 0.21980682957202902 1106 44-45 0.14575612873692023 1106 46-47 -0.3067376558463337 1106 48-49 0.1031570333618177 1106 50-51 0.3461075455820577 1106 52-53 0.20461949416394987 1106 54-55 0.2874882651946322 1106 56-57 0.4181431158347735 1106 58-59 0.5777069016297318 1106 60-61 0.5009188085727274 1106 62-63 0.41684296517248853 1106 64-65 0.5911085517179302 1106 66-67 0.573619816190174 1106 68-69 0.4916633587002188 1106 70-71 0.2149904631818913 1106 72-73 0.5777036359757268 1106 74-75 0.6338062741424828 1106 76-77 0.8374257834276051 1106 78-79 0.9071145319780705 1106 80-81 0.9729782142892134 1106 82-83 1.1112863804541 1106 84-85 1.2217072424404094 1106 86-87 1.3041386959950891 1106 88-89 1.252745927830464 1106 90-91 1.307119467023334 1106 92-93 1.3125483532720672 1106 94-95 1.2164276673336794 1106 96-97 1.2130256053596717 1106 98-99 1.7252109536752194 1106 100-101 1.5619352685435093 1107 1 0.15971382010148716 1107 2 0.08912781700154682 1107 3 0.21010711460340303 1107 4 0.16562164882483188 1107 5 0.2191780932065157 1107 6 0.1307575155542935 1107 7 0.16884202154435712 1107 8 0.15132914898351402 1107 9 0.23617279211352837 1107 10-11 0.18700740479985 1107 12-13 0.2369444089367363 1107 14-15 0.31870140856083395 1107 16-17 0.24976556213777457 1107 18-19 0.22513959949389317 1107 20-21 0.2728544985092256 1107 22-23 0.20482911505298773 1107 24-25 0.35512694330405026 1107 26-27 0.3278852778239596 1107 28-29 0.3351079559579162 1107 30-31 0.3649328877171314 1107 32-33 0.3345290708325521 1107 34-35 0.5227756841440936 1107 36-37 0.380862303429474 1107 38-39 0.5991913050809003 1107 40-41 0.4669932302225561 1107 42-43 0.28825298357904217 1107 44-45 0.39081264363416324 1107 46-47 0.5702908159511111 1107 48-49 0.4924130599619829 1107 50-51 0.5510263282898578 1107 52-53 0.5197816673473383 1107 54-55 0.4858124233526979 1107 56-57 0.3938732088412422 1107 58-59 0.5860046844264133 1107 60-61 0.5863706663496728 1107 62-63 0.7712443045912387 1107 64-65 0.9054321504778429 1107 66-67 0.9231038106066478 1107 68-69 1.2004879952221224 1107 70-71 0.8702661889617822 1107 72-73 1.2596353371211855 1107 74-75 1.2576319301283014 1107 76-77 1.2144368720582293 1107 78-79 1.337860518165641 1107 80-81 1.7133289882975866 1107 82-83 1.8564763405643028 1107 84-85 1.9178741888061488 1107 86-87 2.1916930020538423 1107 88-89 2.233613334784774 1107 90-91 2.4222714455675387 1107 92-93 2.5038565084606184 1107 94-95 2.6298690377023064 1107 96-97 2.3945900380933516 1107 98-99 2.4543072381213342 1107 100-101 1.9610530401522244 1108 1 0.07392039461922195 1108 2 0.13368794411825746 1108 3 0.14187532137268732 1108 4 0.14292579330791 1108 5 0.06301092339007397 1108 6 0.02885236740144137 1108 7 0.06878371451903575 1108 8 0.005765475369720718 1108 9 0.07507356292500589 1108 10-11 0.13989767048511936 1108 12-13 0.0983393644545103 1108 14-15 0.22076015196431342 1108 16-17 0.2742037252569176 1108 18-19 0.020766449324334246 1108 20-21 0.11214394173260445 1108 22-23 0.18947230194488185 1108 24-25 0.20924954584366873 1108 26-27 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942.0 9 1652.0 10 2892.0 11 4473.0 12 6318.0 13 8872.0 14 11977.0 15 15775.0 16 19906.0 17 24627.0 18 24647.0 19 23135.0 20 23909.0 21 26436.0 22 28780.0 23 31891.0 24 35641.0 25 40190.0 26 46181.0 27 54268.0 28 63548.0 29 73716.0 30 85884.0 31 99446.0 32 115886.0 33 137915.0 34 166634.0 35 201021.0 36 232021.0 37 204708.0 38 68842.0 39 1450.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.05169639581914 19.242842255972064 15.43773016182264 23.26773118638615 2 16.085323985706793 20.31898563248286 39.39150900331852 24.204181378491825 3 19.48606955087345 23.58888323940926 30.02193035996149 26.903116849755804 4 13.49745087778331 17.11733180198474 36.34725593866397 33.03796138156798 5 13.944230085395692 37.020544855294375 34.256781500607694 14.77844355870224 6 32.72541129341283 36.22502757170501 16.82022611451784 14.229335020364317 7 28.971148161160993 32.393648583095654 20.092740707088183 18.54246254865517 8 26.445537523524848 35.42563388384749 19.240432697972274 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26.390258853687758 28.81623099795799 22.858230867927432 34-35 22.699923645503883 26.554113893731635 28.61894966256196 22.127012798202525 36-37 22.770475071875644 26.107962340717894 28.622906689835293 22.49865589757117 38-39 22.222290047323934 26.491874302157115 28.60933564162797 22.676500008890986 40-41 22.769420878276843 26.26722521270593 28.60334119098336 22.360012718033865 42-43 22.42062165154521 26.303979983843558 28.460233599670502 22.81516476494073 44-45 22.469918348381125 26.50911133773688 28.636633710397213 22.384336603484783 46-47 22.85212967270282 26.798765188680772 27.76895133651368 22.580153802102725 48-49 22.517697372541235 26.85959076053324 27.832879908373837 22.789831958551687 50-51 22.81031834571893 27.02706432194835 27.689422124661256 22.473195207671463 52-53 23.104690745778797 26.756919993758547 27.639586068832138 22.498803191630515 54-55 22.54449951119268 26.926569570390907 27.885528861922808 22.6434020564936 56-57 22.622093029427155 27.027011248406467 27.642027451758803 22.708868270407574 58-59 22.64154448252766 26.683970410438928 27.639957583625325 23.034527523408084 60-61 22.30412943999983 26.96852420525025 27.942184867884034 22.78516148686589 62-63 22.405818346249664 27.029081116539942 27.94478547143635 22.62031506577404 64-65 22.078372169486247 26.994817099141187 27.73524667142383 23.191564059948735 66-67 22.133662462503263 27.04432841302562 27.733121957536603 23.088887166934512 68-69 22.641321671897117 26.71082960620627 27.766154554896904 22.881694166999704 70-71 22.365912977943957 26.82329557592106 27.450465786795565 23.36032565933942 72-73 22.420063115189997 26.908793986118056 27.388340991861686 23.28280190683026 74-75 22.4110195533543 27.2779164176633 27.142738106484632 23.16832592249777 76-77 22.381563737608655 26.825278715705203 27.6084318997462 23.18472564693994 78-79 22.48394259990298 27.06538341918863 27.488193790607895 22.96248019030049 80-81 22.57614075125718 27.176350392081417 27.23579285363621 23.011716003025196 82-83 22.532078023263466 27.218048823982727 27.27783686701101 22.972036285742796 84-85 22.548408704172157 27.225404471827357 27.16062422574204 23.065562598258442 86-87 22.630847199174777 27.184276389240146 26.99768828189002 23.187188129695055 88-89 22.45682202000023 27.349300331497346 26.751400345402608 23.442477303099814 90-91 22.402990203818426 27.2395413853178 26.995824382580498 23.361644028283273 92-93 22.51882528521873 26.88088323334225 27.096628326136525 23.503663155302498 94-95 22.22717870264346 26.772265351857772 27.147840370665715 23.85271557483305 96-97 22.42059932766437 26.5198935557044 27.177448202876796 23.882058913754435 98-99 22.1681550256929 26.7239878610195 26.85364652384223 24.25421058944537 100-101 22.536231999435298 26.43100940092146 26.992713529567553 24.04004507007569 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 317.0 1 236.5 2 193.0 3 243.0 4 224.5 5 212.0 6 381.0 7 871.5 8 1380.5 9 1596.5 10 1458.5 11 1254.0 12 1296.5 13 1414.5 14 1585.5 15 1797.0 16 1936.5 17 2122.0 18 2428.0 19 2723.5 20 3017.0 21 3431.5 22 4113.0 23 5118.0 24 6413.5 25 8134.5 26 10290.0 27 12797.0 28 15295.0 29 18087.5 30 21277.0 31 25502.0 32 31188.5 33 33892.0 34 34618.5 35 37053.5 36 40936.0 37 45096.0 38 47673.5 39 50041.5 40 53376.5 41 57413.5 42 60121.0 43 62245.0 44 65006.5 45 67736.0 46 69045.5 47 70431.5 48 71194.0 49 69961.5 50 69268.5 51 67977.5 52 66121.5 53 65861.5 54 66004.5 55 61897.5 56 56468.5 57 51922.5 58 47678.5 59 42435.0 60 34744.0 61 28736.5 62 24460.0 63 18761.0 64 13499.5 65 10431.0 66 8229.0 67 6629.0 68 5149.0 69 3812.5 70 2766.5 71 1902.0 72 1391.5 73 1142.5 74 912.5 75 640.0 76 447.5 77 316.5 78 206.0 79 142.0 80 91.5 81 42.5 82 25.5 83 16.0 84 9.0 85 8.0 86 5.5 87 2.0 88 0.5 89 1.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023830020305937127 2 0.010933149628113691 3 0.0016983533402895054 4 0.0 5 4.2458833507237636E-4 6 0.0 7 0.0 8 0.0 9 3.1844125130428227E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0019106475078256935 26-27 0.008597913785215622 28-29 3.4497802224630583E-4 30-31 0.0 32-33 2.9190448036225876E-4 34-35 0.010747392231519527 36-37 0.001114544379564988 38-39 0.013135701616301645 40-41 0.012684576510287244 42-43 0.005307354188404705 44-45 7.695663573186821E-4 46-47 1.8575739659416467E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.961031282607057E-5 66-67 7.430295863766587E-4 68-69 0.001008397295796894 70-71 2.6536770942023523E-5 72-73 2.1229416753618818E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5922062565214114E-4 82-83 0.001167617921449035 84-85 0.006156530858549457 86-87 0.004325493663549835 88-89 0.0 90-91 0.0013533753180431998 92-93 5.307354188404705E-4 94-95 2.6536770942023523E-5 96-97 0.0 98-99 0.0 100-101 5.3073541884047045E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1884178.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.692652705976954 #Duplication Level Percentage of deduplicated Percentage of total 1 79.42913273167966 47.413356348891 2 12.12347755529139 14.473650705934308 3 3.7098586004421543 6.643539030734257 4 1.56641647922086 3.740142195481998 5 0.8385289826717702 2.502700967326107 6 0.5106769584886774 1.829019739680554 7 0.3372689217871306 1.4092733631730945 8 0.2266709498356785 1.0824472229660063 9 0.17471431208734245 0.9386244678774065 >10 0.9887662716225005 10.550190627260413 >50 0.057655258380060165 2.3462896605841683 >100 0.03157236619547183 3.4228124254916112 >500 0.0029423763696076436 1.188591944242579 >1k 0.0021399100869873753 1.904716993937361 >5k 1.7832584058228128E-4 0.5546443064191253 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5406 0.28691556742515834 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5015 0.26616381254849597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3241 0.1720113492461965 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2040 0.10827002544345599 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2016 0.10699626043823886 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2004 0.10635937793563029 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1993 0.10577556897490577 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1936 0.1027503770875151 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.307354188404705E-5 2 0.0 0.0 0.0 0.0 5.307354188404705E-5 3 0.0 0.0 0.0 0.0 5.307354188404705E-5 4 0.0 0.0 0.0 0.0 5.307354188404705E-5 5 0.0 0.0 0.0 0.0 1.061470837680941E-4 6 0.0 0.0 0.0 0.0 1.061470837680941E-4 7 0.0 0.0 0.0 0.0 1.061470837680941E-4 8 0.0 0.0 0.0 0.0 1.061470837680941E-4 9 0.0 0.0 0.0 0.0 1.061470837680941E-4 10-11 0.0 0.0 0.0 1.061470837680941E-4 1.061470837680941E-4 12-13 0.0 0.0 0.0 1.061470837680941E-4 2.122941675361882E-4 14-15 0.0 0.0 0.0 1.061470837680941E-4 2.3883093847821174E-4 16-17 0.0 0.0 0.0 1.061470837680941E-4 2.6536770942023525E-4 18-19 0.0 0.0 0.0 1.3268385471011763E-4 3.1844125130428227E-4 20-21 0.0 0.0 0.0 1.5922062565214114E-4 4.776618769564235E-4 22-23 0.0 0.0 0.0 2.9190448036225876E-4 5.838089607245175E-4 24-25 0.0 0.0 0.0 5.307354188404705E-4 5.838089607245175E-4 26-27 0.0 0.0 0.0 0.001114544379564988 6.368825026085645E-4 28-29 0.0 0.0 0.0 0.0038743685575354345 6.368825026085645E-4 30-31 0.0 0.0 0.0 0.01098622316999774 6.368825026085645E-4 32-33 0.0 0.0 0.0 0.02658984448390757 6.634192735505882E-4 34-35 0.0 0.0 0.0 0.04930532041027971 7.961031282607057E-4 36-37 0.0 0.0 0.0 0.08815515306940215 7.961031282607057E-4 38-39 0.0 0.0 0.0 0.14950816748736054 8.491766701447528E-4 40-41 0.0 0.0 0.0 0.2125329984746664 8.491766701447528E-4 42-43 0.0 0.0 0.0 0.2760620281098707 8.491766701447528E-4 44-45 0.0 0.0 0.0 0.35535389968463704 8.491766701447528E-4 46-47 0.0 0.0 0.0 0.4541768346727326 8.491766701447528E-4 48-49 0.0 0.0 0.0 0.5598727933348123 9.022502120287998E-4 50-51 0.0 0.0 0.0 0.6775633724626866 9.022502120287998E-4 52-53 0.0 0.0 0.0 0.7901588915696924 9.022502120287998E-4 54-55 0.0 0.0 0.0 0.9142979060364785 9.022502120287998E-4 56-57 0.0 0.0 0.0 1.0584191090226083 9.022502120287998E-4 58-59 0.0 0.0 0.0 1.2189931099927926 9.022502120287998E-4 60-61 0.0 0.0 0.0 1.3901287457979024 9.287869829708234E-4 62-63 0.0 2.6536770942023526E-5 0.0 1.5583718735703314 9.553237539128469E-4 64-65 0.0 5.307354188404705E-5 0.0 1.7390342101436276 9.553237539128469E-4 66-67 0.0 5.307354188404705E-5 0.0 1.949099288920686 9.818605248548705E-4 68-69 0.0 5.307354188404705E-5 0.0 2.192600699084694 0.001008397295796894 70-71 0.0 1.061470837680941E-4 0.0 2.4537225251542054 0.0010880076086229645 72-73 0.0 1.061470837680941E-4 0.0 2.716224263312702 0.0011410811505070115 74-75 0.0 1.5922062565214114E-4 0.0 2.979469031057575 0.001167617921449035 76-77 0.0 2.122941675361882E-4 0.0 3.271267364336066 0.001167617921449035 78-79 0.0 2.122941675361882E-4 0.0 3.5987311177606363 0.001167617921449035 80-81 0.0 2.122941675361882E-4 0.0 3.945672861056652 0.001167617921449035 82-83 0.0 2.122941675361882E-4 0.0 4.3004164150096225 0.001220691463333082 84-85 0.0 2.122941675361882E-4 0.0 4.6589281904363595 0.001220691463333082 86-87 0.0 2.122941675361882E-4 0.0 5.034476572807877 0.0012737650052171293 88-89 0.0 2.122941675361882E-4 0.0 5.4414444919747496 0.0013268385471011763 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3220 0.0 54.458805 1 GTATCAA 5830 0.0 42.957565 1 ATCAACG 7550 0.0 32.965744 3 TATCAAC 7635 0.0 32.667885 2 TGGTATC 1475 0.0 32.531204 2 TCAACGC 7745 0.0 32.134895 4 GTGGTAT 1570 0.0 31.782442 1 CAACGCA 7900 0.0 31.564524 5 AACGCAG 8180 0.0 30.367945 6 ACGCAGA 9240 0.0 26.78137 7 CGCAGAG 9400 0.0 26.325516 8 GGACCGA 550 0.0 25.043922 6 GTACATG 6430 0.0 23.945927 1 CGTACAC 200 2.0565494E-8 23.749178 3 TAAGGTG 1215 0.0 23.455357 5 GCAGAGT 10430 0.0 23.407005 9 TACATGG 6500 0.0 23.023321 2 ACATGGG 6615 0.0 22.187439 3 GAGTACT 6570 0.0 21.688171 12-13 ACCGAGT 705 0.0 21.558966 8 >>END_MODULE