FastQCFastQC Report
Thu 26 May 2016
SRR938607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695506
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118161.6989069828297672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81491.1716649460967985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65910.9476553760859001No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55090.7920851868998973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.3015071041802659No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.2665685127087329No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.2536282936452022No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.21380117497189097No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.1644845623186572No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.15082544219604144No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9210.13242157508346442No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8540.12278830089172488No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT8070.11603063093632549No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7420.10668491716821996No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7120.10237151081370972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT250.004996367357.0692631
CGAGTAC250.004997791757.0651553
CGTCACT1155.4569682E-1141.354541
GGTATCA45350.038.9060941
GTATCAA76200.037.384611
ACGCAGT550.002572679434.5625428
GGACCGA902.4022796E-531.6846126
CATATAG1751.4915713E-1029.8912733
TATCAAC95900.029.6532522
ATCAACG95950.029.5386753
TCAACGC97100.029.1888354
CAACGCA98250.028.7987845
AGGTAAG1354.1790918E-728.1803252
GGTAAGG1354.1790918E-728.1803253
AACGCAG100850.028.08736
CATGGGG17800.028.0516914
GTACATG59200.028.0365621
AGGACCG1257.3201463E-626.6304055
ACATGGG60200.025.9889763
TACATGG62900.025.7806282
Produced by FastQC (version 0.11.5)