##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938607_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 695506 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44703568337297 31.0 31.0 34.0 30.0 34.0 2 31.446246330010094 31.0 31.0 34.0 30.0 34.0 3 31.436814348114897 31.0 31.0 34.0 28.0 34.0 4 35.14373851555558 37.0 35.0 37.0 33.0 37.0 5 35.16262404637774 37.0 35.0 37.0 33.0 37.0 6 35.241534940029275 37.0 35.0 37.0 33.0 37.0 7 35.190494402636354 37.0 35.0 37.0 32.0 37.0 8 35.18180001322778 37.0 35.0 37.0 32.0 37.0 9 36.704029871776804 39.0 37.0 39.0 32.0 39.0 10-11 36.564754293996025 39.0 37.0 39.0 32.0 39.0 12-13 36.463340359393015 39.0 37.0 39.0 32.0 39.0 14-15 37.36097747539202 39.5 37.0 41.0 32.0 41.0 16-17 37.279884717026164 39.0 36.5 41.0 32.0 41.0 18-19 37.543067924647666 39.5 37.0 41.0 32.0 41.0 20-21 37.5980897361058 40.0 37.0 41.0 32.0 41.0 22-23 37.49306548038407 40.0 37.0 41.0 32.0 41.0 24-25 37.35139596207654 40.0 36.5 41.0 32.0 41.0 26-27 37.14307353207593 39.0 36.0 41.0 31.5 41.0 28-29 36.93486540734372 39.0 36.0 41.0 31.0 41.0 30-31 36.59098268023568 39.0 35.0 40.5 30.5 41.0 32-33 36.19657558669516 38.0 35.0 40.0 30.0 41.0 34-35 35.9158648523521 38.0 35.0 40.0 30.0 41.0 36-37 35.60530390823372 38.0 35.0 40.0 29.0 41.0 38-39 35.33934646142521 38.0 35.0 40.0 27.0 41.0 40-41 35.16658734791648 38.0 35.0 40.0 26.5 41.0 42-43 34.78422400381881 38.0 34.5 40.0 24.5 41.0 44-45 34.2784239100741 38.0 33.5 40.0 23.5 41.0 46-47 33.68219540881027 38.0 33.0 40.0 19.0 41.0 48-49 33.19685092580079 38.0 33.0 40.0 13.5 41.0 50-51 32.456292972310806 37.0 32.0 39.5 9.0 40.5 52-53 32.49510500268869 37.0 32.5 39.5 7.5 40.5 54-55 32.662325702438224 37.0 33.0 40.0 3.5 41.0 56-57 32.411404790181535 37.0 32.5 40.0 2.0 41.0 58-59 32.1250887842808 37.0 32.0 40.0 2.0 41.0 60-61 31.731649331565798 36.0 31.0 40.0 2.0 41.0 62-63 31.244260293944265 36.0 31.0 39.0 2.0 41.0 64-65 30.807310792430258 35.0 30.0 39.0 2.0 40.0 66-67 30.352784160021624 35.0 29.0 39.0 2.0 40.0 68-69 29.884385612776885 35.0 28.5 38.0 2.0 40.0 70-71 29.33293817738452 35.0 28.0 38.0 2.0 40.0 72-73 28.754506071838346 34.0 26.5 37.0 2.0 39.0 74-75 28.149232357449108 34.0 26.0 36.5 2.0 39.0 76-77 27.484267569222983 34.0 25.5 36.0 2.0 39.0 78-79 26.765193254982705 33.0 23.5 35.5 2.0 37.5 80-81 26.12177105589312 32.5 21.0 35.0 2.0 37.0 82-83 25.442220484079215 32.0 19.0 35.0 2.0 36.5 84-85 24.746851932262267 31.5 13.5 35.0 2.0 36.0 86-87 24.05826405523461 31.0 6.0 34.5 2.0 35.5 88-89 23.324184119188043 31.0 2.0 34.0 2.0 35.0 90-91 22.48501594522549 30.0 2.0 34.0 2.0 35.0 92-93 21.615151414941064 29.0 2.0 34.0 2.0 35.0 94-95 20.721165597421155 29.0 2.0 34.0 2.0 35.0 96-97 19.589239345167403 27.0 2.0 34.0 2.0 35.0 98-99 18.232645009532625 25.0 2.0 33.0 2.0 35.0 100-101 14.68420401836936 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.537165222412753 1101 2 0.5784137019899056 1101 3 0.5158375734914387 1101 4 0.2390423064356355 1101 5 0.42823613601493093 1101 6 0.5244331611777611 1101 7 0.48956348518580484 1101 8 0.6575200760709023 1101 9 0.6644501233753815 1101 10-11 0.7812667562737161 1101 12-13 0.9421828703034336 1101 14-15 1.093771238675565 1101 16-17 0.9264831487385052 1101 18-19 1.0641690265766073 1101 20-21 1.0341851036193646 1101 22-23 1.0278284148756356 1101 24-25 1.1255254970349071 1101 26-27 1.0806642114400304 1101 28-29 0.9476812660360849 1101 30-31 0.9641789610950937 1101 32-33 1.1758795661697121 1101 34-35 1.2275410453632318 1101 36-37 0.9865160824693433 1101 38-39 1.3863326035483823 1101 40-41 1.4871299585876372 1101 42-43 1.5854156445234722 1101 44-45 1.4720763769003753 1101 46-47 1.5565781862282293 1101 48-49 1.4243744538870544 1101 50-51 1.360309868161714 1101 52-53 1.3700253211454552 1101 54-55 1.5489973625288798 1101 56-57 1.700714429756495 1101 58-59 1.5113759699366156 1101 60-61 1.4201046897726144 1101 62-63 1.5032028004413611 1101 64-65 1.639874054027505 1101 66-67 1.7029387569872405 1101 68-69 1.5047364096618594 1101 70-71 1.5556555852177354 1101 72-73 1.5664294426724688 1101 74-75 1.9190759438464866 1101 76-77 2.069264688658315 1101 78-79 2.1228312269533482 1101 80-81 1.9623769198161227 1101 82-83 2.2521656416781184 1101 84-85 1.8613225744288222 1101 86-87 1.967306593753424 1101 88-89 1.7225412097078383 1101 90-91 1.8025278052515006 1101 92-93 1.6320105361325297 1101 94-95 1.6729803868358708 1101 96-97 1.7094093440374962 1101 98-99 1.7576775057469582 1101 100-101 1.777424735105937 1102 1 0.5657603292306419 1102 2 0.44533229231274873 1102 3 0.4518893950251126 1102 4 0.3823391881555409 1102 5 0.3555970746399524 1102 6 0.33101571369272875 1102 7 0.40367185423785656 1102 8 0.4444206902556189 1102 9 0.4329976804085476 1102 10-11 0.4996840829513616 1102 12-13 0.6220131117732208 1102 14-15 0.7906848517343619 1102 16-17 0.7792648594676521 1102 18-19 0.6897180961181704 1102 20-21 0.5723964778757988 1102 22-23 0.5586814116235033 1102 24-25 0.57866914667418 1102 26-27 0.5990643834697451 1102 28-29 0.7951051694674902 1102 30-31 0.5768209701701466 1102 32-33 0.678938409028305 1102 34-35 0.6874873585636152 1102 36-37 0.9098915856320886 1102 38-39 0.6871055291648673 1102 40-41 1.0028409583411886 1102 42-43 1.1868733063691224 1102 44-45 1.1710280104577748 1102 46-47 1.17507452093232 1102 48-49 0.9254109812064399 1102 50-51 1.0029386850708804 1102 52-53 0.9216571872328885 1102 54-55 0.7140782309889673 1102 56-57 0.7676260132513093 1102 58-59 0.8997052521958082 1102 60-61 0.7563551186388153 1102 62-63 0.7609103112777795 1102 64-65 0.7614787626159405 1102 66-67 0.7095263834121646 1102 68-69 0.7400269030875215 1102 70-71 0.7693541756372255 1102 72-73 0.7215557830303503 1102 74-75 0.6634137019514554 1102 76-77 0.8315459668970391 1102 78-79 0.8331399622933695 1102 80-81 0.9017340312633486 1102 82-83 1.0121262101493258 1102 84-85 1.343106029616532 1102 86-87 1.5532477557271243 1102 88-89 1.4979397532540624 1102 90-91 1.4562853979953303 1102 92-93 1.1577666695232054 1102 94-95 1.467320319473675 1102 96-97 1.8351565376820602 1102 98-99 2.3041484805658676 1102 100-101 2.386766286562631 1103 1 0.5190238895352195 1103 2 0.4271578806309897 1103 3 0.4268682437701372 1103 4 0.31681730052354595 1103 5 0.43901135722071416 1103 6 0.4752516050222795 1103 7 0.3859204676555805 1103 8 0.5227900068350735 1103 9 0.6770048248324514 1103 10-11 0.6366032068193874 1103 12-13 0.6189344291047192 1103 14-15 0.5608098791526501 1103 16-17 0.738936466649676 1103 18-19 0.8736791778090165 1103 20-21 0.657878883165175 1103 22-23 0.8406903108181822 1103 24-25 0.8451906269486784 1103 26-27 0.8083756359373879 1103 28-29 0.8799217019150802 1103 30-31 0.8140048764152539 1103 32-33 0.8413173768470585 1103 34-35 0.7296096911553462 1103 36-37 0.6996872801766258 1103 38-39 0.9570973820148012 1103 40-41 1.0953972833498113 1103 42-43 1.0543332756606247 1103 44-45 0.9690805282404966 1103 46-47 0.6593677556642419 1103 48-49 0.5063967863642063 1103 50-51 0.7743562891820588 1103 52-53 0.5940712504673158 1103 54-55 0.4846106159104764 1103 56-57 0.472863690686836 1103 58-59 0.15778973187493506 1103 60-61 0.40055967477580623 1103 62-63 0.5079804707739122 1103 64-65 0.6482177091267687 1103 66-67 0.5898024464585703 1103 68-69 0.5159881101191388 1103 70-71 0.44414187970766505 1103 72-73 0.5799207126828456 1103 74-75 0.6044252019671212 1103 76-77 0.2581354445393771 1103 78-79 0.5593330497498918 1103 80-81 0.7081922144488821 1103 82-83 0.4188256743175316 1103 84-85 0.6478329434134196 1103 86-87 0.7617286255920739 1103 88-89 0.6321248815716771 1103 90-91 1.0067898945954603 1103 92-93 0.722223160608344 1103 94-95 0.7289848449021825 1103 96-97 1.272462651178131 1103 98-99 1.6588706940838698 1103 100-101 1.813575110552856 1104 1 0.4282047658664432 1104 2 0.3336082028491454 1104 3 0.30196933599178166 1104 4 0.4190417550511967 1104 5 0.4171647960588558 1104 6 0.4444089002626441 1104 7 0.4641722319808821 1104 8 0.5060938283338743 1104 9 0.563960310202809 1104 10-11 0.7193658124873537 1104 12-13 0.5911106573220053 1104 14-15 0.787629964793723 1104 16-17 0.9287610556831893 1104 18-19 1.0932706375381258 1104 20-21 0.8839466298514793 1104 22-23 0.8795565455905034 1104 24-25 0.8147629323621146 1104 26-27 0.8632165699869532 1104 28-29 0.7846162118625557 1104 30-31 0.877098128137014 1104 32-33 0.7668602952837347 1104 34-35 0.8327141800756408 1104 36-37 0.9532595600509737 1104 38-39 0.8190234974344506 1104 40-41 1.0131805756787244 1104 42-43 1.2818141851926725 1104 44-45 0.7844563359141361 1104 46-47 0.8715645854121803 1104 48-49 0.3254342607187084 1104 50-51 0.3015862771786857 1104 52-53 0.360574821683052 1104 54-55 0.35820915847988033 1104 56-57 0.5970794278726004 1104 58-59 0.7601950882729014 1104 60-61 0.8621978369830714 1104 62-63 0.7481702687597327 1104 64-65 0.7308398498130906 1104 66-67 0.8178771397766944 1104 68-69 1.126783056785012 1104 70-71 0.9754613141020059 1104 72-73 1.030681351841075 1104 74-75 0.8045292737489831 1104 76-77 0.686297183580983 1104 78-79 0.7969710672356562 1104 80-81 1.1617046902931634 1104 82-83 1.1659291161352314 1104 84-85 1.5290698014246047 1104 86-87 1.5331878439257345 1104 88-89 1.6902944100349906 1104 90-91 1.5930832791204779 1104 92-93 1.8363754331614857 1104 94-95 2.03866543604693 1104 96-97 2.3224362607876365 1104 98-99 2.183250945558754 1104 100-101 2.222942209397635 1105 1 0.5225641636940495 1105 2 0.37875525233145524 1105 3 0.4061505593926569 1105 4 0.3517102426329828 1105 5 0.4519262773521291 1105 6 0.5505036725423906 1105 7 0.4290693291034131 1105 8 0.42112749409008643 1105 9 0.8092836285030032 1105 10-11 0.7351148755630135 1105 12-13 0.6330459619018143 1105 14-15 0.8131889433873809 1105 16-17 0.6696816235546237 1105 18-19 0.5784981989940121 1105 20-21 0.8035894869943405 1105 22-23 0.8305863683688841 1105 24-25 0.8635504887363723 1105 26-27 0.772243597872361 1105 28-29 0.6123690226355976 1105 30-31 0.7171500559779602 1105 32-33 0.7446678923109857 1105 34-35 0.8120343541212449 1105 36-37 0.7263809473342135 1105 38-39 0.7872633500673629 1105 40-41 0.8837738552315386 1105 42-43 0.7841961962452046 1105 44-45 0.6571894553958089 1105 46-47 0.6175429567518194 1105 48-49 0.6277432234322902 1105 50-51 0.5734174084751302 1105 52-53 0.5227064039735936 1105 54-55 0.6325374313630618 1105 56-57 0.6879849096740358 1105 58-59 0.8747023295865048 1105 60-61 0.8090789905116225 1105 62-63 0.6499242228980755 1105 64-65 0.8917248463194731 1105 66-67 0.8533333742936229 1105 68-69 0.660254282091497 1105 70-71 0.7775082555998161 1105 72-73 0.8984320982045411 1105 74-75 1.1771353526411872 1105 76-77 1.3551460370396597 1105 78-79 1.3566523541568252 1105 80-81 1.499422247302629 1105 82-83 1.208320972833448 1105 84-85 1.1165015171077641 1105 86-87 1.575722621287099 1105 88-89 1.5877369241682544 1105 90-91 1.4436597257951895 1105 92-93 1.6866858886019997 1105 94-95 1.7203890592445426 1105 96-97 1.8865798484138807 1105 98-99 1.9044524319336524 1105 100-101 1.8153523281805857 1106 1 0.10745852390486732 1106 2 0.1010122499049082 1106 3 -0.0613673305935869 1106 4 -0.1689926831332329 1106 5 0.07616701551692273 1106 6 -0.02826249799593228 1106 7 0.3123304069847421 1106 8 0.3110924869031777 1106 9 0.31291765270665195 1106 10-11 0.187635895330871 1106 12-13 0.0576307828473972 1106 14-15 0.12850478085511696 1106 16-17 -0.027700372086862046 1106 18-19 0.10273178889215728 1106 20-21 0.1476597100202497 1106 22-23 0.10744936121288617 1106 24-25 0.34759343243121776 1106 26-27 0.16647340112115216 1106 28-29 0.29137417711544344 1106 30-31 0.16650265485586857 1106 32-33 -0.02580126581640485 1106 34-35 -0.0010896702369578293 1106 36-37 -0.13244465041542952 1106 38-39 0.07778243014340802 1106 40-41 0.3305366564247194 1106 42-43 -0.1524353859559966 1106 44-45 0.31790470709207597 1106 46-47 0.25753789500625857 1106 48-49 0.46780172330092284 1106 50-51 0.17660456941545988 1106 52-53 0.1540036404923555 1106 54-55 0.4190979172906424 1106 56-57 -0.0273382692567381 1106 58-59 0.056974435118100075 1106 60-61 0.6252178446251548 1106 62-63 0.5479851106741549 1106 64-65 0.24343405483248048 1106 66-67 -0.21388834561797054 1106 68-69 -0.12866802977385206 1106 70-71 -0.2825120686610134 1106 72-73 0.11158181768336917 1106 74-75 0.35527498726816376 1106 76-77 0.399671044381126 1106 78-79 0.5087819580269368 1106 80-81 0.39845298098525816 1106 82-83 -0.03904962422069502 1106 84-85 0.2783218521875952 1106 86-87 0.11450293380158882 1106 88-89 -0.12010858826586457 1106 90-91 -0.024760391359105682 1106 92-93 -0.33941397847888055 1106 94-95 -0.5762118433690198 1106 96-97 -0.39749130195473725 1106 98-99 -0.028690023360706363 1106 100-101 -0.10381912975669394 1107 1 0.12510714310270288 1107 2 0.011469552956203444 1107 3 -0.07516103945625474 1107 4 0.049609358442545215 1107 5 0.016734603354485955 1107 6 0.3006453219377718 1107 7 0.3126204794456058 1107 8 0.07553838170992577 1107 9 0.16232424460332595 1107 10-11 0.10651865834739027 1107 12-13 0.014327926206163966 1107 14-15 0.28475841868820595 1107 16-17 0.5925842805990769 1107 18-19 0.23904008248365471 1107 20-21 0.26454364477685033 1107 22-23 0.2653260689667931 1107 24-25 0.17775028148094663 1107 26-27 0.33213053928915315 1107 28-29 0.33669227401108515 1107 30-31 0.452331851794078 1107 32-33 0.441629512780068 1107 34-35 0.26647450352408697 1107 36-37 0.3004989934671869 1107 38-39 0.27149739959992303 1107 40-41 0.472461287202556 1107 42-43 0.4903184322181815 1107 44-45 0.3266030641750888 1107 46-47 0.12134792044484044 1107 48-49 0.3260803340828389 1107 50-51 0.46999377986494295 1107 52-53 0.36741300334437454 1107 54-55 0.43505953612397263 1107 56-57 0.337546169309924 1107 58-59 0.27454595610922894 1107 60-61 0.10291947838195625 1107 62-63 0.19300893669174712 1107 64-65 0.31924601917646456 1107 66-67 0.46935634386136726 1107 68-69 0.40742595370346635 1107 70-71 0.5597390844724366 1107 72-73 0.8918118527783214 1107 74-75 0.7330523833716498 1107 76-77 0.7502760935503119 1107 78-79 0.6203224527149835 1107 80-81 0.6314441433170579 1107 82-83 0.733536429019054 1107 84-85 1.0520519741134464 1107 86-87 0.5724777318481777 1107 88-89 0.26193306384947235 1107 90-91 0.5040731929250732 1107 92-93 0.3337058462562865 1107 94-95 0.5201695026817035 1107 96-97 0.3278577868933148 1107 98-99 0.5352432828737541 1107 100-101 0.4829058567415796 1108 1 0.2829067622719421 1108 2 0.039765810400837154 1108 3 0.10365648483976031 1108 4 -0.054129016147456355 1108 5 0.1506980937710054 1108 6 0.18371849693368603 1108 7 0.2316711390581645 1108 8 0.19264764061023243 1108 9 0.2581362464732706 1108 10-11 0.41623155300321457 1108 12-13 0.33129256823666253 1108 14-15 0.37066578027598496 1108 16-17 0.5530445841234695 1108 18-19 0.5768687010409934 1108 20-21 0.3853471900755707 1108 22-23 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1019.0 9 1346.0 10 1871.0 11 2826.0 12 4248.0 13 5892.0 14 7226.0 15 9019.0 16 11407.0 17 13832.0 18 11209.0 19 7966.0 20 7881.0 21 8240.0 22 9136.0 23 10233.0 24 11326.0 25 12269.0 26 13955.0 27 15873.0 28 18500.0 29 21070.0 30 24674.0 31 29497.0 32 35039.0 33 42445.0 34 53850.0 35 69588.0 36 79847.0 37 91161.0 38 52946.0 39 3425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.869447308293466 22.118308838437216 15.85891745069027 19.153326402579047 2 14.607841302786442 23.766727071479668 41.346254877604686 20.279176748129203 3 17.05818591682297 32.045079294703946 30.820355066382415 20.076379722090667 4 10.703478814157101 19.645381494885285 40.32551082396907 29.325628866988552 5 10.475600711420897 41.3133924263917 34.31252115181059 13.898485710376809 6 25.299611264548794 41.35391045411957 18.857339414550882 14.489138866780749 7 24.422606472100018 36.90011692407701 23.397759027099635 15.279517576723336 8 25.19404650788195 34.840354770372905 21.463693851634417 18.501904870110724 9 27.99562233586636 17.23769968418691 22.236347843094393 32.530330136852335 10-11 22.5044451733186 29.98355717442342 27.85936852644662 19.65262912581135 12-13 25.103903072711294 28.755220329181046 28.378666098898176 17.762210499209488 14-15 22.547849298440404 28.921283805162606 28.68901263900315 19.841854257393845 16-17 18.074732481877806 32.324746864572546 29.75707628581291 19.84344436773674 18-19 17.962738154484338 29.483906323265856 34.83765365153428 17.71570187071552 20-21 19.27092399787656 28.70404071937954 34.992497471583185 17.03253781116071 22-23 19.158153322181967 28.715655972189662 34.77414167796905 17.35204902765932 24-25 18.401191665533837 29.612902900980565 34.92504979058823 17.060855642897373 26-27 17.779328302918266 29.707238474762804 35.041180828795646 17.47225239352328 28-29 17.707526371251113 28.99100518253977 35.76865948088287 17.532808965326254 30-31 17.94863309590594 29.752834272995216 35.003909962238474 17.29462266886037 32-33 17.35884341509027 30.16449687117888 34.7473207221463 17.72933899158455 34-35 18.043217571894406 29.75702254970582 34.83374564518832 17.366014233211448 36-37 18.159670623235044 29.585214873167793 34.128459240056905 18.126655263540254 38-39 17.577767677871808 30.042673700803707 34.606904308504824 17.772654312819657 40-41 17.487764972401518 29.765224114061738 35.11814308060195 17.628867832934787 42-43 17.48898272906143 29.757562507910574 34.069328262895674 18.684126500132322 44-45 17.39853764801461 30.059026105211693 33.86408897771962 18.678347269054072 46-47 18.100546287788262 30.063659279774406 33.11993741920362 18.715857013233713 48-49 17.874166900330003 30.44834602304999 32.39069948018894 19.28678759643106 50-51 18.204589719827073 30.39418617274826 32.08137805191061 19.319846055514056 52-53 17.964153655129376 30.52259682891635 32.326417572747054 19.18683194320722 54-55 17.979602621390285 30.466939638282653 32.207654948539215 19.345802791787843 56-57 18.158784026689293 30.73467450856329 31.358407270531053 19.748134194216362 58-59 17.85989576742099 29.848943293335328 32.285782492148044 20.00537844709564 60-61 18.297145610193684 30.66655161379628 31.49867149888722 19.537631277122816 62-63 18.488619639201904 30.324309905878444 32.20213953802716 18.984930916892488 64-65 18.43937671697248 29.807684703280557 32.18191478424293 19.57102379550404 66-67 18.166569365317198 30.09084974275057 31.641536076752068 20.10104481518016 68-69 18.38483290469949 29.910637572770092 32.0683781823097 19.636151340220714 70-71 18.59190601967943 29.504835720691013 31.909931690815906 19.993326568813647 72-73 18.073252587168394 29.55236287881675 31.66553167559918 20.70885285841568 74-75 17.997044430621425 29.990184057903274 31.488104013030394 20.52466749844491 76-77 17.779490767472915 29.36661966331603 32.44575858877773 20.408130980433327 78-79 17.825692169325634 29.01665261155481 32.22819175054801 20.929463468571548 80-81 17.918467586321928 29.91611524735506 31.372379859607346 20.79303730671567 82-83 18.325454996423296 29.010996121341964 31.41547652854714 21.2480723536876 84-85 17.954458365415793 28.784489991351215 31.488762691892646 21.772288951340347 86-87 17.94367943032332 29.06888290682358 31.57746774573922 21.409969917113884 88-89 18.336631982900723 28.33247510909996 31.787426967035557 21.543465940963763 90-91 18.29296796060717 28.393672236098055 31.61429608049438 21.69906372280039 92-93 18.60381163049247 28.428518624522326 31.470761247416558 21.49690849756865 94-95 18.132255811661945 27.58425750044413 31.972861529210967 22.31062515868295 96-97 18.635002031782108 27.40920810842884 31.51829862522071 22.437491234568345 98-99 18.507750753430617 27.360371742774365 31.556931741451233 22.574945762343788 100-101 18.93283163302013 26.76331352432479 32.14174712577521 22.162107716879863 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3566.0 1 2246.5 2 1295.0 3 1589.0 4 1272.0 5 1274.0 6 2153.5 7 3936.5 8 5805.0 9 6106.0 10 4629.0 11 3294.0 12 2944.5 13 2781.5 14 2625.0 15 2492.5 16 2356.5 17 2294.0 18 2291.0 19 2380.5 20 2555.0 21 2749.5 22 2984.5 23 3233.5 24 3556.0 25 3980.0 26 4773.5 27 5642.0 28 6311.0 29 7710.0 30 10111.5 31 12424.0 32 15937.0 33 20335.5 34 23499.5 35 26892.5 36 32792.0 37 36727.5 38 36546.0 39 35540.5 40 33026.5 41 29601.5 42 26021.0 43 24731.0 44 24676.5 45 23795.5 46 21624.0 47 19025.0 48 19030.0 49 17586.5 50 13975.5 51 11030.5 52 9049.0 53 8279.5 54 8015.0 55 7522.0 56 6759.0 57 6319.0 58 6151.5 59 5708.0 60 4926.0 61 4494.5 62 4031.0 63 2698.5 64 1621.0 65 1264.5 66 1005.0 67 759.5 68 571.5 69 465.5 70 368.0 71 307.0 72 272.5 73 251.5 74 211.5 75 148.0 76 106.0 77 77.0 78 49.0 79 32.5 80 24.5 81 12.0 82 6.0 83 6.0 84 6.0 85 3.5 86 2.5 87 2.0 88 2.0 89 1.0 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007907911649935442 2 0.10769137865093903 3 0.1177559934781296 4 0.12135049877355479 5 0.16117761744686604 6 0.09906456594191855 7 0.026311778762512474 8 0.026311778762512474 9 0.02271727346708727 10-11 0.05319867837229298 12-13 0.05564294197318211 14-15 0.028468481939767597 16-17 0.03249432787064382 18-19 0.03795797591969013 20-21 0.05851854620952228 22-23 0.03235054765882681 24-25 0.049532282970959275 26-27 0.057655864938620224 28-29 0.05513971123182259 30-31 0.06994907304897442 32-33 0.05118575540685486 34-35 0.06347896351720905 36-37 0.05391757943137802 38-39 0.033069448717911856 40-41 0.03860498687286666 42-43 0.03364456956517989 44-45 0.00733279080266741 46-47 0.012221318004445684 48-49 0.00877059292083749 50-51 0.008698702814928986 52-53 0.009417603874014028 54-55 0.010567845568550092 56-57 0.013802900334432773 58-59 0.019841669230747108 60-61 0.024945866750250897 62-63 0.010855405992184108 64-65 0.008554922603111978 66-67 0.01035217525082458 68-69 0.01143052683945214 70-71 0.030409514799297203 72-73 0.028684152257493104 74-75 0.02997817416384618 76-77 0.023795625055714833 78-79 0.004241516248601738 80-81 0.004025845930876225 82-83 0.00481663709586977 84-85 0.004672856884052762 86-87 0.013515339910798757 88-89 0.03910821761422619 90-91 0.036304503483794534 92-93 0.030625185117022714 94-95 0.047519360005521157 96-97 0.043637294286461945 98-99 0.026815009503872 100-101 0.07706619353391632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 695506.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.53624060058143 #Duplication Level Percentage of deduplicated Percentage of total 1 76.55609558529586 26.439597365748874 2 11.02031413377082 7.612004408357942 3 4.0439153571739865 4.1898490123124095 4 2.056947697914858 2.8415696239199852 5 1.1830756429255445 2.0429492526382083 6 0.7860974383440497 1.6289310159690495 7 0.5344392817739916 1.2920266535223954 8 0.41451392709155 1.1452602174660513 9 0.31874126219737664 0.9907312428523449 >10 2.40491978998389 17.186384096568062 >50 0.39648609734394974 9.603464584561285 >100 0.2694606144379743 16.69225318469935 >500 0.010828401816672847 2.371559663504174 >1k 0.0024988619576937336 1.3513344880289735 >5k 0.0012494309788468668 2.912524965204756 >10k+ 4.1647699294895563E-4 1.6995602246461257 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11816 1.6989069828297672 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8149 1.1716649460967985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6591 0.9476553760859001 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5509 0.7920851868998973 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2097 0.3015071041802659 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1854 0.2665685127087329 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1764 0.2536282936452022 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.21380117497189097 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1144 0.1644845623186572 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1049 0.15082544219604144 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 921 0.13242157508346442 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 854 0.12278830089172488 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 807 0.11603063093632549 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 742 0.10668491716821996 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 712 0.10237151081370972 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.875604236340161E-4 0.0 14-15 0.0 0.0 0.0 5.032307413595281E-4 0.0 16-17 0.0 0.0 0.0 5.751208472680322E-4 0.0 18-19 0.0 0.0 0.0 5.751208472680322E-4 0.0 20-21 0.0 0.0 0.0 8.626812709020483E-4 0.0 22-23 0.0 0.0 0.0 0.0028756042363401613 0.0 24-25 0.0 0.0 0.0 0.007907911649935442 0.0 26-27 0.0 0.0 0.0 0.017972526477126008 0.0 28-29 0.0 0.0 0.0 0.0339321299888139 0.0 30-31 0.0 0.0 0.0 0.08109203946479254 0.0 32-33 0.0 0.0 0.0 0.15305403547920507 0.0 34-35 0.0 0.0 0.0 0.250321348773411 0.0 36-37 0.0 0.0 0.0 0.3941734506963276 0.0 38-39 0.0 0.0 0.0 0.6008575051832766 0.0 40-41 0.0 0.0 0.0 0.8304745034550385 0.0 42-43 0.0 0.0 0.0 1.0457134805450996 0.0 44-45 0.0 0.0 0.0 1.3059556639338843 0.0 46-47 0.0 0.0 0.0 1.6117042843627516 0.0 48-49 0.0 0.0 0.0 1.946065166943204 0.0 50-51 0.0 0.0 0.0 2.283373543865905 0.0 52-53 0.0 0.0 0.0 2.606447679818722 0.0 54-55 0.0 0.0 0.0 2.959787550359019 0.0 56-57 0.0 0.0 0.0 3.3464125399349536 0.0 58-59 0.0 0.0 0.0 3.760715220285663 0.0 60-61 0.0 0.0 0.0 4.211753744755617 0.0 62-63 0.0 0.0 0.0 4.656537830011532 0.0 64-65 0.0 0.0 0.0 5.097871190183837 0.0 66-67 0.0 0.0 0.0 5.590462195868907 0.0 68-69 0.0 0.0 0.0 6.119070144614137 0.0 70-71 0.0 0.0 0.0 6.69368776114081 0.0 72-73 0.0 0.0 0.0 7.274056586140163 0.0 74-75 0.0 0.0 0.0 7.846158048960038 0.0 76-77 0.0 0.0 0.0 8.437741730481118 0.0 78-79 0.0 0.0 0.0 9.10746995712474 0.0 80-81 0.0 0.0 0.0 9.820835478054827 0.0 82-83 0.0 0.0 0.0 10.542324580952574 0.0 84-85 0.0 0.0 0.0 11.253533398705404 0.0 86-87 0.0 0.0 0.0 11.966683249317764 0.0 88-89 0.0 7.189010590850403E-5 0.0 12.692341978358202 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGAGT 25 0.0049963673 57.069263 1 CGAGTAC 25 0.0049977917 57.065155 3 CGTCACT 115 5.4569682E-11 41.35454 1 GGTATCA 4535 0.0 38.906094 1 GTATCAA 7620 0.0 37.38461 1 ACGCAGT 55 0.0025726794 34.562542 8 GGACCGA 90 2.4022796E-5 31.684612 6 CATATAG 175 1.4915713E-10 29.891273 3 TATCAAC 9590 0.0 29.653252 2 ATCAACG 9595 0.0 29.538675 3 TCAACGC 9710 0.0 29.188835 4 CAACGCA 9825 0.0 28.798784 5 AGGTAAG 135 4.1790918E-7 28.180325 2 GGTAAGG 135 4.1790918E-7 28.180325 3 AACGCAG 10085 0.0 28.0873 6 CATGGGG 1780 0.0 28.051691 4 GTACATG 5920 0.0 28.036562 1 AGGACCG 125 7.3201463E-6 26.630405 5 ACATGGG 6020 0.0 25.988976 3 TACATGG 6290 0.0 25.780628 2 >>END_MODULE