FastQCFastQC Report
Thu 26 May 2016
SRR938607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695506
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106701.534134860087476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80701.160306309363255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66830.9608831555730647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59430.8544857988284789No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21480.3088398949829333No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17370.24974622792614298No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.23349906399082104No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.1984166923074711No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12150.17469295735766477No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.15470750791510066No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.1518319036787605No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA9060.13026487190620928No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT8700.125088784280797No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8600.12365098216262692No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG7260.10438443377914786No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7260.10438443377914786No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG7150.10280285144916076No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7140.10265907123734376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCGC200.002076696871.2243356
CGACCGT252.8315257E-547.4880118-19
AGATAGT601.4951347E-647.4828876
GTTCGAC301.4962225E-647.48288716-17
GGTATCA47900.041.4658161
CGTCACT951.9203071E-840.014361
GTATCAA76250.034.9600871
GATAGTC702.0568502E-433.916357
TACCTGG1605.2750693E-1132.6679732
GGTACTC1601.904482E-929.6853433
TCGACCG404.50077E-429.68000618-19
GTATGGT1153.8509042E-628.923421
TCGTATC504.9441664E-528.50817994-95
TGGTACT1754.9349183E-927.15262
ATGCCGC350.00838042827.15064694-95
ATCAACG98750.027.0309473
TATCAAC98900.026.9055082
TCAACGC100250.026.673874
GGATATG2500.026.6095491
CAACGCA101400.026.3713595
Produced by FastQC (version 0.11.5)