##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938607_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 695506 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.735210048511444 31.0 31.0 34.0 30.0 34.0 2 31.932239261774882 31.0 31.0 34.0 30.0 34.0 3 31.94774883322358 31.0 31.0 34.0 30.0 34.0 4 35.67472602680638 37.0 35.0 37.0 33.0 37.0 5 35.600614516625306 37.0 35.0 37.0 33.0 37.0 6 35.62308305032595 37.0 35.0 37.0 33.0 37.0 7 35.56382259822345 37.0 35.0 37.0 33.0 37.0 8 35.5566494034559 37.0 35.0 37.0 33.0 37.0 9 37.08722282769667 39.0 37.0 39.0 33.0 39.0 10-11 36.99571966309421 39.0 37.0 39.0 33.0 39.0 12-13 36.88969469709822 39.0 37.0 39.0 32.5 39.0 14-15 37.760817304236056 39.5 37.0 41.0 32.0 41.0 16-17 37.72664218568927 39.0 37.0 41.0 32.5 41.0 18-19 38.06969961438148 40.0 37.0 41.0 33.0 41.0 20-21 38.10531109724431 40.0 37.0 41.0 33.0 41.0 22-23 37.962768833051044 40.0 37.0 41.0 33.0 41.0 24-25 37.80306208717107 40.0 37.0 41.0 32.0 41.0 26-27 37.56860544696954 39.5 36.0 41.0 32.0 41.0 28-29 37.41237602551236 39.0 36.0 41.0 32.0 41.0 30-31 37.03184803581853 39.0 35.5 40.5 31.5 41.0 32-33 36.67401575256001 38.5 35.0 40.0 31.0 41.0 34-35 36.400097195423186 38.0 35.0 40.0 30.0 41.0 36-37 36.242240181968235 38.0 35.0 40.0 30.0 41.0 38-39 36.00155642079292 38.0 35.0 40.0 30.0 41.0 40-41 35.74807909637013 38.0 35.0 40.0 29.5 41.0 42-43 35.40973046961493 38.0 35.0 40.0 28.0 41.0 44-45 34.91294755185433 38.0 34.5 40.0 25.5 41.0 46-47 34.294949288719295 38.0 33.5 40.0 23.5 41.0 48-49 33.78546626484891 38.0 33.0 40.0 20.0 41.0 50-51 33.38801174971891 38.0 33.0 40.0 16.5 41.0 52-53 32.9280904837629 37.0 33.0 40.0 13.0 41.0 54-55 32.625932774124166 37.0 32.0 40.0 8.5 41.0 56-57 32.31107208277139 37.0 31.5 40.0 7.5 41.0 58-59 31.86473732793103 36.0 31.0 39.5 2.0 41.0 60-61 31.498674346447046 36.0 31.0 39.0 2.0 40.5 62-63 31.023810721977956 35.0 30.0 39.0 2.0 40.0 64-65 30.50787484220122 35.0 29.0 38.5 2.0 40.0 66-67 30.038451860947283 35.0 28.5 38.0 2.0 40.0 68-69 29.577591710208107 34.5 28.0 38.0 2.0 40.0 70-71 29.076647074216467 34.0 27.5 37.0 2.0 39.5 72-73 28.61569130963644 34.0 26.0 36.5 2.0 39.0 74-75 28.05782696339068 33.5 26.0 36.0 2.0 39.0 76-77 26.804527207529482 31.5 25.0 35.0 2.0 37.0 78-79 27.46238062647914 33.0 26.0 35.0 2.0 37.5 80-81 27.093348583621133 33.0 25.5 35.0 2.0 37.0 82-83 26.666198422443514 33.0 24.5 35.0 2.0 36.5 84-85 26.099089008577927 32.5 23.0 35.0 2.0 36.0 86-87 25.544120395798167 32.0 20.5 35.0 2.0 36.0 88-89 24.838166744787248 31.5 18.0 34.0 2.0 35.0 90-91 24.28039945018447 31.0 11.0 34.0 2.0 35.0 92-93 23.536278623045668 31.0 4.0 34.0 2.0 35.0 94-95 22.81956086647707 30.5 2.0 34.0 2.0 35.0 96-97 21.860099697198876 29.5 2.0 34.0 2.0 35.0 98-99 20.672668531975283 29.0 2.0 34.0 2.0 35.0 100-101 17.008204817787338 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37159606687200863 1101 2 0.40919155503860694 1101 3 0.39379384231402526 1101 4 0.3434352325655965 1101 5 0.29917561591519615 1101 6 0.34589235293129406 1101 7 0.2482613465031278 1101 8 0.3354151574546407 1101 9 0.3663439936703341 1101 10-11 0.3213603938439036 1101 12-13 0.42507054132402544 1101 14-15 0.481220577451964 1101 16-17 0.5698515290918635 1101 18-19 0.7066293750155666 1101 20-21 0.5095896187447977 1101 22-23 0.5361654039627979 1101 24-25 0.41168546867061906 1101 26-27 0.4908003709436599 1101 28-29 0.32875165343649115 1101 30-31 0.6307042310439144 1101 32-33 0.4539022905263934 1101 34-35 0.4560307235859966 1101 36-37 0.4270114590830971 1101 38-39 0.2525136056765689 1101 40-41 0.4203965103547276 1101 42-43 0.5296271474377008 1101 44-45 0.298529379483476 1101 46-47 0.20638378014461267 1101 48-49 -0.07739881369776924 1101 50-51 0.25153158758196525 1101 52-53 0.35897537803231216 1101 54-55 0.4558062300930459 1101 56-57 0.3799098108940129 1101 58-59 0.6767816345887105 1101 60-61 0.6844085953487387 1101 62-63 0.5218550652598459 1101 64-65 0.3327598859562073 1101 66-67 0.5143126951545263 1101 68-69 0.4341202659772172 1101 70-71 0.25628119137039107 1101 72-73 0.4372992152916275 1101 74-75 0.04009545732350972 1101 76-77 0.20631108372136353 1101 78-79 0.3133843313655724 1101 80-81 0.39834584999652733 1101 82-83 0.4959158950897411 1101 84-85 0.7546891303830847 1101 86-87 0.6860813309003149 1101 88-89 0.9209129691304021 1101 90-91 0.657110365513919 1101 92-93 0.8355728757999685 1101 94-95 0.8906535000838609 1101 96-97 0.4919929952805724 1101 98-99 0.6351411560910485 1101 100-101 1.2890270109407815 1102 1 0.10190325587756988 1102 2 0.23102241504218668 1102 3 0.24623004393671266 1102 4 0.07773631355003374 1102 5 0.25619718244865197 1102 6 0.1873689090511519 1102 7 0.2464999749044594 1102 8 0.3271809264419332 1102 9 0.1474748473919263 1102 10-11 0.44756303131062225 1102 12-13 0.2515065786486801 1102 14-15 0.299492452044241 1102 16-17 0.19625108258779278 1102 18-19 0.2650711531069092 1102 20-21 0.2689014515860393 1102 22-23 0.4690508640697857 1102 24-25 0.49448684684120536 1102 26-27 0.314721198619182 1102 28-29 0.18443274128278375 1102 30-31 0.3612349137022619 1102 32-33 0.477872890842427 1102 34-35 0.27496220647242353 1102 36-37 0.23607901940221865 1102 38-39 0.4464698540758647 1102 40-41 0.3866888352202693 1102 42-43 0.3782579165941229 1102 44-45 0.42410744279620616 1102 46-47 0.1706235876259754 1102 48-49 0.2596356550638532 1102 50-51 0.43326575335868966 1102 52-53 0.1866726444029183 1102 54-55 0.2791338935333485 1102 56-57 0.3563632444813578 1102 58-59 0.3393762871708468 1102 60-61 0.05298345484975542 1102 62-63 0.2887064866794766 1102 64-65 0.47212811168238034 1102 66-67 0.3974255147504486 1102 68-69 0.32410460934078245 1102 70-71 0.4939648789914983 1102 72-73 0.5942835731520795 1102 74-75 0.6505895111842719 1102 76-77 0.38915305317159365 1102 78-79 0.6851678348762356 1102 80-81 0.8875698317111222 1102 82-83 0.8204419677384642 1102 84-85 0.7515988776057227 1102 86-87 0.5684064863794092 1102 88-89 0.6176308742337895 1102 90-91 0.5684015064671364 1102 92-93 1.073598448306143 1102 94-95 0.8144784066997524 1102 96-97 1.2110433526288027 1102 98-99 1.1966248640077985 1102 100-101 1.6046362908804745 1103 1 0.2921235422812316 1103 2 0.31692784543105645 1103 3 0.29893583781381494 1103 4 0.2809450372676352 1103 5 0.12935232298698907 1103 6 0.13704458980821244 1103 7 0.23551698991401793 1103 8 0.28453372937490684 1103 9 0.38819179659758163 1103 10-11 0.38002002375883137 1103 12-13 0.4247275230913843 1103 14-15 0.7055182095725172 1103 16-17 0.5614235828228047 1103 18-19 0.513151872562922 1103 20-21 0.3657350303783815 1103 22-23 0.34135212061100617 1103 24-25 0.5635652788281718 1103 26-27 0.3894086300734543 1103 28-29 0.33831987191920376 1103 30-31 0.37380815971485504 1103 32-33 0.4110975955082381 1103 34-35 0.4980217746734681 1103 36-37 0.58829441751584 1103 38-39 0.40830358954417534 1103 40-41 0.64709995294659 1103 42-43 0.6917038477193742 1103 44-45 0.7494215781015683 1103 46-47 0.9193231771349559 1103 48-49 0.7817974699982102 1103 50-51 0.8756011652597948 1103 52-53 0.853375868794231 1103 54-55 0.6905571379791837 1103 56-57 0.9317008811811363 1103 58-59 0.9826251199349265 1103 60-61 1.0354122456246806 1103 62-63 0.7215865042271865 1103 64-65 0.6590112000789574 1103 66-67 0.9393755037831326 1103 68-69 0.8070817659854121 1103 70-71 0.6350985862581311 1103 72-73 0.5823291971924398 1103 74-75 0.6531041603867891 1103 76-77 0.6712951269346377 1103 78-79 0.7227935673363639 1103 80-81 0.750713378228216 1103 82-83 0.6726416982558128 1103 84-85 0.7233972043064405 1103 86-87 0.7712470215845002 1103 88-89 0.6328127750265686 1103 90-91 0.7203615227614328 1103 92-93 1.0324161714910502 1103 94-95 0.6737442231381969 1103 96-97 0.9137977160543684 1103 98-99 1.2265186377050377 1103 100-101 1.3004812443477114 1104 1 0.2595846164552498 1104 2 0.37705962712293584 1104 3 0.2797261874439272 1104 4 0.33215942088175865 1104 5 0.3322816563316735 1104 6 0.24690735977765144 1104 7 0.2620137932715565 1104 8 0.1860060577735254 1104 9 0.1836151921044049 1104 10-11 0.4483338906320853 1104 12-13 0.43217535950501684 1104 14-15 0.5435408031519913 1104 16-17 0.4453133135556868 1104 18-19 0.5462569148301171 1104 20-21 0.5147726324283255 1104 22-23 0.789994079629281 1104 24-25 0.387016758818298 1104 26-27 0.6982357900417853 1104 28-29 0.5251042454028223 1104 30-31 0.6547434436669448 1104 32-33 0.7183370755324177 1104 34-35 0.5918183303018623 1104 36-37 0.7932455952934347 1104 38-39 0.5169277137662931 1104 40-41 0.5899541162435185 1104 42-43 0.8778252690547248 1104 44-45 0.9814431245784476 1104 46-47 0.8412799820248367 1104 48-49 0.6688104183156085 1104 50-51 0.4617721749901875 1104 52-53 0.2790901119433755 1104 54-55 0.18331893490938 1104 56-57 0.07279901938165523 1104 58-59 0.4463500384226684 1104 60-61 0.4227433684080957 1104 62-63 0.5877340179970112 1104 64-65 0.3709983964830563 1104 66-67 0.21313962477030302 1104 68-69 0.44263685805382025 1104 70-71 0.1644653445817461 1104 72-73 0.2856213984063949 1104 74-75 0.4144935293159371 1104 76-77 0.5766036391125269 1104 78-79 0.6234507731899228 1104 80-81 0.597832143498465 1104 82-83 0.7963147298306374 1104 84-85 0.8997158725281551 1104 86-87 1.1178337225302997 1104 88-89 1.0694949921598926 1104 90-91 0.9164368034785717 1104 92-93 1.1456990509380418 1104 94-95 1.3791295654045364 1104 96-97 1.6463438779551538 1104 98-99 1.7150068939812684 1104 100-101 1.5524945757514281 1105 1 0.20565588622594433 1105 2 0.17026136757900545 1105 3 0.17611664669565386 1105 4 0.1389942045951571 1105 5 0.10588738586226043 1105 6 0.25160786158797777 1105 7 0.3272808961109135 1105 8 0.3955992102857522 1105 9 0.20446572885089154 1105 10-11 0.28496366259422956 1105 12-13 0.39947828779060046 1105 14-15 0.4720725315392116 1105 16-17 0.5016637550217382 1105 18-19 0.36395763896147315 1105 20-21 0.3732970559816451 1105 22-23 0.43032629077074347 1105 24-25 0.34157592797284764 1105 26-27 0.2742576727833139 1105 28-29 0.4506386098822759 1105 30-31 0.5040336043732907 1105 32-33 0.39412552119080146 1105 34-35 0.44212084570553145 1105 36-37 0.4588549137206641 1105 38-39 0.43289433385140796 1105 40-41 0.5282600299829596 1105 42-43 0.6670847812188612 1105 44-45 0.6729091729809156 1105 46-47 0.5981282638008665 1105 48-49 0.7021625430694698 1105 50-51 0.6170832656336529 1105 52-53 0.7493626140077794 1105 54-55 0.7159763064395861 1105 56-57 1.018012765173431 1105 58-59 0.9397240776782105 1105 60-61 0.9610381660959568 1105 62-63 0.823850863579171 1105 64-65 0.6541043800948216 1105 66-67 0.5683689330342929 1105 68-69 0.6203110624621324 1105 70-71 0.9084785568030505 1105 72-73 0.9442514244497175 1105 74-75 0.8678855130547412 1105 76-77 0.8210416433048628 1105 78-79 1.2107991184862463 1105 80-81 1.1154749053608768 1105 82-83 1.3362094449421171 1105 84-85 1.2559535654710494 1105 86-87 1.0115325092926675 1105 88-89 1.1600233222113445 1105 90-91 1.4275171248912635 1105 92-93 1.2816443917244271 1105 94-95 1.4431024422386827 1105 96-97 1.2786725319601118 1105 98-99 1.4804979058595613 1105 100-101 1.7682293467607657 1106 1 -0.0022118437936846647 1106 2 0.029527453110915758 1106 3 0.09906799211535855 1106 4 0.1136932046106125 1106 5 5.702346590368279E-4 1106 6 0.004057591885811007 1106 7 -0.054567916117648 1106 8 -0.034063777921538474 1106 9 -0.2843327441247965 1106 10-11 -0.07337797818412639 1106 12-13 -0.02140199226689532 1106 14-15 0.08528368747188608 1106 16-17 0.062165138307932466 1106 18-19 -0.03844630379057179 1106 20-21 -0.13239450983649448 1106 22-23 0.027788076523663108 1106 24-25 0.0994312934231445 1106 26-27 -0.1158744349120795 1106 28-29 0.06337160473552217 1106 30-31 -0.11850700550909465 1106 32-33 -0.3022518531928995 1106 34-35 -0.17221181530435103 1106 36-37 -0.055550775526789664 1106 38-39 -0.2501003262002115 1106 40-41 -0.05180055640674652 1106 42-43 -0.12291556653465108 1106 44-45 -0.22879056890869975 1106 46-47 -0.8055700948025546 1106 48-49 -0.27646124172520103 1106 50-51 0.07279588985893781 1106 52-53 0.20272307211507723 1106 54-55 0.2909809431530874 1106 56-57 0.253401321764521 1106 58-59 -0.3493856346909965 1106 60-61 -0.1950799773639602 1106 62-63 -0.18510282338084139 1106 64-65 0.06842854046328384 1106 66-67 -0.01332029897139364 1106 68-69 -0.32515539091571455 1106 70-71 -0.28800024568840854 1106 72-73 0.20022409102364946 1106 74-75 0.2706748826162624 1106 76-77 -0.11111840831721054 1106 78-79 0.026607606763086267 1106 80-81 -0.22120072561855153 1106 82-83 -0.027551354736406353 1106 84-85 -0.06109910759428061 1106 86-87 0.41966375919606946 1106 88-89 0.37011570872778066 1106 90-91 0.34233393606883666 1106 92-93 0.2863419620865528 1106 94-95 0.5205529453220983 1106 96-97 0.6015075731495827 1106 98-99 0.616173938687492 1106 100-101 0.9466008170626132 1107 1 0.08546637424538517 1107 2 0.050822970155536495 1107 3 0.12160585897591147 1107 4 0.1533529801479645 1107 5 -0.04664478346095535 1107 6 0.007460218186601253 1107 7 -0.07870118151295458 1107 8 -0.10337010398207269 1107 9 0.1449096133246286 1107 10-11 0.20183876510365906 1107 12-13 0.07955753689970635 1107 14-15 0.08792869030582295 1107 16-17 0.1086836008475558 1107 18-19 0.12119946430031803 1107 20-21 0.18744186639511184 1107 22-23 0.08826723042810158 1107 24-25 0.1879310653727586 1107 26-27 0.04362915950554935 1107 28-29 0.0968291730504447 1107 30-31 0.13470144399635586 1107 32-33 -0.012202292786263058 1107 34-35 -0.14135631146565686 1107 36-37 0.11865872930805921 1107 38-39 0.08565854724994892 1107 40-41 0.0012273611592377165 1107 42-43 -0.12118944455536962 1107 44-45 -0.3277711168784734 1107 46-47 -0.10362241089757163 1107 48-49 -0.01846850498694863 1107 50-51 -0.10137831010404597 1107 52-53 0.014263757640264885 1107 54-55 -0.23904441910352148 1107 56-57 -0.2616385536569865 1107 58-59 -0.3040293703662833 1107 60-61 -0.03353442549156327 1107 62-63 -0.03880420523261208 1107 64-65 -0.4658381773477345 1107 66-67 -0.35028144643698056 1107 68-69 -0.31966500624528393 1107 70-71 -0.06324561454798783 1107 72-73 0.10903160695378133 1107 74-75 -0.2873901814000597 1107 76-77 -0.33867643712217443 1107 78-79 0.0057262063237786265 1107 80-81 -0.04524124416124309 1107 82-83 -0.10099980103578687 1107 84-85 -0.09512632478794814 1107 86-87 0.09037903590039065 1107 88-89 0.1953508678000837 1107 90-91 0.290362302102114 1107 92-93 0.5213235558438853 1107 94-95 0.5846456005728875 1107 96-97 0.5349722024388832 1107 98-99 0.2951925395840469 1107 100-101 0.07141403520664724 1108 1 0.1613227683634122 1108 2 0.08286102282572116 1108 3 0.056826622993867204 1108 4 0.15375395464902653 1108 5 0.03583244080731873 1108 6 0.20027194260499925 1108 7 0.08606133136193961 1108 8 -0.030736708697226334 1108 9 0.11553015462120442 1108 10-11 0.08370046500749595 1108 12-13 0.03158882431289811 1108 14-15 0.060711833413812144 1108 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2316 70-71 -0.6935960926853895 2316 72-73 -0.6731936304953372 2316 74-75 -0.8885089545466265 2316 76-77 -0.8665530895484892 2316 78-79 -0.6402334696153069 2316 80-81 -0.606105238633063 2316 82-83 -0.6373837721855864 2316 84-85 -1.1131729976210316 2316 86-87 -1.1019574490248765 2316 88-89 -1.069869629664364 2316 90-91 -0.9889521816642493 2316 92-93 -0.8976612164604916 2316 94-95 -0.8085553826950047 2316 96-97 -1.2175381539057497 2316 98-99 -1.658143625023346 2316 100-101 -1.8973873641145662 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 16.0 5 59.0 6 153.0 7 290.0 8 631.0 9 1030.0 10 1770.0 11 2754.0 12 3884.0 13 5329.0 14 6540.0 15 8677.0 16 10874.0 17 13840.0 18 11701.0 19 7889.0 20 7225.0 21 7593.0 22 8122.0 23 8919.0 24 9901.0 25 11321.0 26 12789.0 27 14806.0 28 17665.0 29 20363.0 30 23857.0 31 28325.0 32 33807.0 33 41772.0 34 53551.0 35 70116.0 36 85610.0 37 104344.0 38 57388.0 39 2586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.1544721525685 23.100801720766523 16.451522924382893 19.293203202282086 2 14.713142699315549 24.435304982780796 40.45635931031109 20.395193007592564 3 16.973829125845068 31.917194100410352 31.331577297679676 19.777399476064907 4 10.945556184993372 20.455035614358465 39.483627747280394 29.115780453367762 5 10.34465893725366 41.80082856238672 34.40777928018643 13.446733220173194 6 25.294102423271692 41.57332934582879 18.464111021328357 14.668457209571162 7 24.292241907330776 36.97610085319178 22.999945363519508 15.731711875957934 8 25.130768102647572 35.566767216961466 21.17753117873893 18.124933501652034 9 27.227658711786813 17.427599474339544 22.11670352232763 33.228038291546014 10-11 22.374932782750975 30.249127973015327 27.707740839043804 19.66819840518989 12-13 24.79331594551305 28.858557654427138 28.290913378173588 18.057213021886223 14-15 22.150204311680994 29.310171299744358 28.376390714098797 20.16323367447585 16-17 17.948946522388017 32.6446500820985 29.62555319436497 19.780850201148517 18-19 17.723067809623497 29.73691096841724 34.52292287916999 18.01709834278928 20-21 18.921116424588718 29.048491314237406 34.71544458279296 17.31494767838092 22-23 18.93693224788859 29.077606807130348 34.54211753744756 17.443343407533508 24-25 18.160903177845313 29.91902429414966 34.76591960233257 17.154152925672456 26-27 17.5664678132014 29.996750478441726 34.8554322366917 17.581349471665177 28-29 17.391019912854752 29.19962530849864 35.60388810728312 17.80546667136349 30-31 17.685317651456874 29.948669652549626 34.94582994845398 17.420182747539524 32-33 17.05581004799411 30.44513171780958 34.646054216954084 17.85300401724222 34-35 17.692948158416584 29.991645180448284 34.8774171096926 17.437989551442538 36-37 17.922557569107017 29.731712105206764 34.031264898790695 18.31446542689552 38-39 17.417505072131387 30.28907194733898 34.38869133977601 17.904731640753624 40-41 17.41232156619161 29.879611097691434 34.99861969012043 17.709447645996526 42-43 17.432407561841114 29.81256434293996 34.120234308949634 18.634793786269288 44-45 17.288645206933147 30.131125241213503 33.801165302066885 18.77906424978647 46-47 17.99819124493534 30.222888084358722 32.863267894166256 18.91565277653967 48-49 17.708833568653613 30.550635077195597 32.24860748864855 19.49192386550224 50-51 18.082373772556597 30.411121199769088 31.99206902079869 19.514436006875624 52-53 17.920190480024615 30.736255330651353 32.0393353903489 19.304218798975135 54-55 17.80584577713523 30.593959540959048 31.967660373533324 19.632534308372403 56-57 18.03291416608915 30.86040954355534 30.996569404146047 20.110106886209465 58-59 17.580974665821977 30.2241180569388 31.88420387286242 20.3107034043768 60-61 18.137011039444666 31.242649236670854 30.884277058716965 19.736062665167516 62-63 18.173837519466346 30.85777021731847 31.666836824933025 19.301555438282158 64-65 18.145788694114096 30.37130243460504 31.51015976646199 19.97274910481888 66-67 17.743817523385818 30.752523353466476 30.922700079875938 20.580959043271765 68-69 18.103115407486555 30.46228140124668 31.226123202656442 20.20847998861032 70-71 18.310913205637334 30.09111352022844 31.047180038705633 20.550793235428593 72-73 17.795545829616014 30.0669081696694 30.656910693013927 21.48063530770066 74-75 17.548595491074767 30.4426518555165 30.572374060161188 21.436378593247543 76-77 17.24558831779936 29.877737386408263 31.87387343323604 21.002800862556338 78-79 17.17923990297246 29.33925336543613 31.715967905831793 21.76553882575962 80-81 17.153234806820535 30.34134316680857 30.793442368318107 21.711979658052787 82-83 17.647930323963365 29.456465063091763 30.761524271181322 22.13408034176355 84-85 17.10305593524158 29.150419659914768 30.805115730937345 22.94140867390631 86-87 17.002681069235432 29.49607545818675 30.87507540282544 22.626168069752374 88-89 17.207863927545837 28.8802113126525 30.84108450995079 23.070840249850878 90-91 17.05274118541285 28.791165961106586 31.03922636002189 23.116866493458677 92-93 17.087324604012977 29.03176165777028 30.533013959769573 23.34789977844717 94-95 16.680773520632084 28.006575687646208 31.13168130926946 24.180969482452248 96-97 16.965016476964614 27.935179578057372 30.80552291168318 24.294281033294833 98-99 16.648048711189116 27.993324869536444 30.729265196171866 24.62936122310257 100-101 16.55453526404986 27.681105337368482 31.303967057311045 24.460392341270612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3663.0 1 2300.5 2 1205.0 3 1514.0 4 1356.5 5 1348.5 6 2144.0 7 3963.5 8 5620.0 9 5726.5 10 4431.0 11 3176.0 12 2843.0 13 2797.0 14 2623.5 15 2392.0 16 2267.0 17 2263.5 18 2313.5 19 2273.5 20 2370.5 21 2581.0 22 2709.0 23 3009.0 24 3376.0 25 3758.0 26 4437.0 27 5325.5 28 6038.5 29 7436.5 30 9978.5 31 12152.0 32 15627.0 33 20120.5 34 23527.5 35 26995.0 36 33029.0 37 37094.5 38 36742.0 39 35608.0 40 33159.5 41 30126.0 42 26627.5 43 25373.0 44 25512.0 45 24678.5 46 22165.5 47 19539.0 48 19613.0 49 17904.5 50 14409.0 51 11472.0 52 9143.5 53 8157.5 54 7908.5 55 7485.0 56 6816.0 57 6183.5 58 5924.5 59 5631.5 60 4792.5 61 4263.0 62 3696.5 63 2400.5 64 1391.0 65 1044.0 66 818.5 67 615.0 68 477.5 69 372.0 70 294.5 71 245.5 72 200.5 73 203.0 74 175.0 75 123.5 76 91.5 77 56.5 78 43.0 79 27.5 80 13.5 81 9.0 82 4.5 83 5.0 84 3.5 85 1.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.875604236340161E-4 2 0.05061063455958683 3 0.0 4 0.0 5 0.04687234905234462 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.007260900696758906 26-27 0.002731824024523153 28-29 7.189010590850403E-5 30-31 0.0015815823299870884 32-33 5.751208472680322E-4 34-35 0.014234240969883799 36-37 0.027246350139323024 38-39 0.005607428260863314 40-41 0.0014378021181700805 42-43 0.0020129229654381126 44-45 0.009201933556288516 46-47 0.0 48-49 0.0 50-51 7.907911649935442E-4 52-53 0.0 54-55 0.0017972526477126008 56-57 0.0 58-59 0.025952328232969956 60-61 0.0 62-63 0.010783515886275604 64-65 0.016678504570772936 66-67 0.007548461120392922 68-69 0.020057339548472622 70-71 0.0 72-73 7.189010590850403E-5 74-75 0.0250177568561594 76-77 0.05219221688957393 78-79 0.03443536073017343 80-81 0.027893361092499562 82-83 0.04148059110920682 84-85 0.017469295735766478 86-87 0.0107116257803671 88-89 0.08792159952610042 90-91 0.028109031410225072 92-93 0.026815009503872 94-95 0.03127219607019925 96-97 0.042127602062383356 98-99 0.055858612290907626 100-101 0.01675039467668144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 695506.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.85212778401231 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95821143168656 24.62536740414779 2 11.681381305695686 7.675164626969744 3 4.3884054454763985 4.3250536938853825 4 2.188115306134301 2.87536974573109 5 1.2741736376311703 2.09296575812395 6 0.8509363124392495 1.6773041083386266 7 0.5904933082614572 1.3579273133026692 8 0.4252541018305381 1.1176401675209775 9 0.3502053835038421 1.035449280856553 >10 2.54320632584076 17.298392646813358 >50 0.44023067493571344 10.089688342350602 >100 0.29232016982317116 17.301537588568262 >500 0.012252941250073042 2.54732895047563 >1k 0.0030632353125182605 1.4715536507177913 >5k 0.0013128151339363973 2.9753101167697746 >10k+ 4.376050446454658E-4 1.5339466054277877 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10670 1.534134860087476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8070 1.160306309363255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6683 0.9608831555730647 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5943 0.8544857988284789 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2148 0.3088398949829333 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1737 0.24974622792614298 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1624 0.23349906399082104 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1380 0.1984166923074711 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1215 0.17469295735766477 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1076 0.15470750791510066 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1056 0.1518319036787605 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 906 0.13026487190620928 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 870 0.125088784280797 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 860 0.12365098216262692 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 726 0.10438443377914786 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 726 0.10438443377914786 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 715 0.10280285144916076 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 714 0.10265907123734376 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.875604236340161E-4 0.0 14-15 0.0 0.0 0.0 5.032307413595281E-4 0.0 16-17 0.0 0.0 0.0 5.751208472680322E-4 0.0 18-19 0.0 0.0 0.0 6.470109531765362E-4 0.0 20-21 0.0 0.0 0.0 0.0010064614827190563 0.0 22-23 0.0 0.0 0.0 0.0030193844481571693 0.0 24-25 0.0 0.0 0.0 0.008195472073569458 0.0 26-27 0.0 0.0 0.0 0.018331977006668526 0.0 28-29 0.0 0.0 0.0 0.03457914094199044 0.0 30-31 0.0 0.0 0.0 0.08209850094751159 0.0 32-33 0.0 0.0 0.0 0.15434805738555815 0.0 34-35 0.0 0.0 0.0 0.2525499420565746 0.0 36-37 0.0 0.0 0.0 0.39755228567402723 0.0 38-39 0.0 0.0 0.0 0.6053865818555124 0.0 40-41 0.0 0.0 0.0 0.8367289426690783 0.0 42-43 0.0 0.0 0.0 1.0535495020891266 0.0 44-45 0.0 0.0 0.0 1.3150857073842641 0.0 46-47 0.0 0.0 0.0 1.6232067013081122 0.0 48-49 0.0 0.0 0.0 1.9574238036767475 0.0 50-51 0.0 0.0 0.0 2.293797609222638 0.0 52-53 0.0 0.0 0.0 2.6186689978231676 0.0 54-55 0.0 0.0 0.0 2.9716494178339223 0.0 56-57 0.0 0.0 0.0 3.3582025173039485 0.0 58-59 0.0 0.0 0.0 3.771858186701481 0.0 60-61 0.0 0.0 0.0 4.225700425301866 0.0 62-63 0.0 0.0 0.0 4.671419081934592 0.0 64-65 0.0 0.0 0.0 5.111889760835996 0.0 66-67 0.0 0.0 0.0 5.604840217050608 0.0 68-69 0.0 0.0 0.0 6.135461088761276 0.0 70-71 0.0 0.0 0.0 6.711085166770667 0.0 72-73 0.0 0.0 0.0 7.293466914735459 0.0 74-75 0.0 0.0 0.0 7.867796970838498 0.0 76-77 0.0 0.0 0.0 8.46642588273861 0.0 78-79 0.0 0.0 0.0 9.140323735524927 0.0 80-81 0.0 0.0 0.0 9.859224794609968 0.0 82-83 0.0 0.0 0.0 10.587902908098563 0.0 84-85 0.0 0.0 0.0 11.30701963750133 0.0 86-87 0.0 0.0 0.0 12.033972388448124 0.0 88-89 0.0 0.0 0.0 12.778394435130682 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTCGC 20 0.0020766968 71.224335 6 CGACCGT 25 2.8315257E-5 47.48801 18-19 AGATAGT 60 1.4951347E-6 47.482887 6 GTTCGAC 30 1.4962225E-6 47.482887 16-17 GGTATCA 4790 0.0 41.465816 1 CGTCACT 95 1.9203071E-8 40.01436 1 GTATCAA 7625 0.0 34.960087 1 GATAGTC 70 2.0568502E-4 33.91635 7 TACCTGG 160 5.2750693E-11 32.667973 2 GGTACTC 160 1.904482E-9 29.685343 3 TCGACCG 40 4.50077E-4 29.680006 18-19 GTATGGT 115 3.8509042E-6 28.92342 1 TCGTATC 50 4.9441664E-5 28.508179 94-95 TGGTACT 175 4.9349183E-9 27.1526 2 ATGCCGC 35 0.008380428 27.150646 94-95 ATCAACG 9875 0.0 27.030947 3 TATCAAC 9890 0.0 26.905508 2 TCAACGC 10025 0.0 26.67387 4 GGATATG 250 0.0 26.609549 1 CAACGCA 10140 0.0 26.371359 5 >>END_MODULE