FastQCFastQC Report
Thu 26 May 2016
SRR938606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691570
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116701.6874647541102132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82191.1884552539873041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66640.963604551961479No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53690.7763494657084604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.29700536460517374No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.2586867562213514No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.24162413060138527No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15280.2209465419263415No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.17265063551050508No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.1548650172795234No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9230.13346443599346414No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA9140.1321630492936362No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT8030.11611261332909177No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG7570.10946108130774904No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7460.10787049756351491No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7210.10425553450843733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.042.0815161
GTATCAA74900.039.3632131
GGTAAGG1401.0913936E-1137.3397063
CATGGGT4550.031.3340234
GGTGTGC2051.8189894E-1230.1258078
AGGTAAG1601.893568E-929.7020422
ATCAACG97950.029.6929453
GTACATG60650.029.4497871
TCAACGC99350.029.3223574
TATCAAC99950.029.1938842
ATAAGCC1153.8469407E-628.9272083
CAACGCA100900.028.9148315
AACGCAG102500.028.5520576
GGATGAC3050.028.0363738
CGTCACT856.302922E-428.017721
TAAGGTG2400.027.7178925
TACATGG63750.027.5075842
CATGGGA39400.027.2595394
ACATGGG62300.027.1561533
ATGGGAT15400.027.1522225
Produced by FastQC (version 0.11.5)