##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938606_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 691570 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37392165652067 31.0 31.0 34.0 30.0 34.0 2 31.43054209985974 31.0 31.0 34.0 28.0 34.0 3 31.44820625533207 31.0 31.0 34.0 28.0 34.0 4 35.13043943490897 37.0 35.0 37.0 33.0 37.0 5 35.187236288445135 37.0 35.0 37.0 33.0 37.0 6 35.27812658154634 37.0 35.0 37.0 33.0 37.0 7 35.23146753040184 37.0 35.0 37.0 32.0 37.0 8 35.20578827884379 37.0 35.0 37.0 32.0 37.0 9 36.72667553537603 39.0 37.0 39.0 32.0 39.0 10-11 36.60000867591133 39.0 37.0 39.0 32.0 39.0 12-13 36.49338172563876 39.0 37.0 39.0 32.0 39.0 14-15 37.41770247408071 39.5 37.0 41.0 32.0 41.0 16-17 37.362006738291136 39.0 37.0 41.0 32.0 41.0 18-19 37.51020287172665 39.5 37.0 41.0 32.0 41.0 20-21 37.628501091718846 40.0 37.0 41.0 32.0 41.0 22-23 37.53200688288966 40.0 37.0 41.0 32.0 41.0 24-25 37.411205662478125 40.0 37.0 41.0 32.0 41.0 26-27 37.2153845597698 39.0 36.0 41.0 32.0 41.0 28-29 37.02865364315977 39.0 36.0 41.0 31.0 41.0 30-31 36.70208438769755 39.0 35.5 40.5 31.0 41.0 32-33 36.29705235912489 38.0 35.0 40.0 30.0 41.0 34-35 36.02112656708648 38.0 35.0 40.0 30.0 41.0 36-37 35.694734444813975 38.0 35.0 40.0 29.5 41.0 38-39 35.411152884017525 38.0 35.0 40.0 28.5 41.0 40-41 35.196858597105134 38.0 35.0 40.0 27.0 41.0 42-43 34.89017669939413 38.0 34.5 40.0 25.5 41.0 44-45 34.429779342655124 38.0 34.0 40.0 23.5 41.0 46-47 33.8522774267247 38.0 33.0 40.0 20.0 41.0 48-49 33.367802970053646 38.0 33.0 40.0 14.5 41.0 50-51 32.58593200977486 37.5 32.0 39.5 9.0 40.5 52-53 32.64394132191969 37.5 33.0 39.5 8.0 40.5 54-55 32.819170149081074 38.0 33.0 40.0 4.5 41.0 56-57 32.593634773052614 37.5 33.0 40.0 2.0 41.0 58-59 32.33789565770638 37.0 32.0 40.0 2.0 41.0 60-61 31.958208135112862 36.5 31.5 40.0 2.0 41.0 62-63 31.510745839177524 36.0 31.0 39.5 2.0 41.0 64-65 31.08406668883844 35.5 30.5 39.0 2.0 40.5 66-67 30.618238211605476 35.0 29.5 39.0 2.0 40.0 68-69 30.123827667481237 35.0 29.0 38.5 2.0 40.0 70-71 29.573290483971256 35.0 28.5 38.0 2.0 40.0 72-73 29.089183307546598 34.5 28.0 37.0 2.0 39.5 74-75 28.570146912098558 34.0 26.5 36.5 2.0 39.0 76-77 27.91946585305898 34.0 26.0 36.0 2.0 39.0 78-79 27.21273623783565 33.5 25.0 35.5 2.0 38.0 80-81 26.61426825917839 33.0 23.5 35.0 2.0 37.0 82-83 26.008240669780356 32.5 21.0 35.0 2.0 36.5 84-85 25.359513136775742 32.0 19.0 35.0 2.0 36.0 86-87 24.75525543328947 32.0 14.0 35.0 2.0 36.0 88-89 24.04559336003586 31.0 4.5 34.5 2.0 35.0 90-91 23.289190537472706 31.0 2.0 34.0 2.0 35.0 92-93 22.543276891710164 30.0 2.0 34.0 2.0 35.0 94-95 21.716072848735486 29.5 2.0 34.0 2.0 35.0 96-97 20.626601790129705 29.0 2.0 34.0 2.0 35.0 98-99 19.349198924186993 27.5 2.0 34.0 2.0 35.0 100-101 16.002736527032695 13.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4255467233729071 1101 2 0.44032615328542946 1101 3 0.5390372359204925 1101 4 0.42899736800968213 1101 5 0.3910326092933474 1101 6 0.53696360866563 1101 7 0.3418960368330275 1101 8 0.496343793257644 1101 9 0.31662852590274326 1101 10-11 0.3966766862550415 1101 12-13 0.36297470339728477 1101 14-15 0.4265664060609424 1101 16-17 0.5762346712896047 1101 18-19 0.4878331812717036 1101 20-21 0.620702118716757 1101 22-23 0.5465886156186741 1101 24-25 0.5249827717456839 1101 26-27 0.6406540649411951 1101 28-29 0.5984077411819229 1101 30-31 0.5276889322388101 1101 32-33 0.5905121953321171 1101 34-35 0.7464187117518222 1101 36-37 0.7631211579775865 1101 38-39 0.8281793422896726 1101 40-41 0.8181358121143845 1101 42-43 0.8253552448665928 1101 44-45 1.035631919446402 1101 46-47 1.1328438756337462 1101 48-49 1.2472235282503519 1101 50-51 1.07771316534825 1101 52-53 0.9956309614472332 1101 54-55 1.199317041501203 1101 56-57 1.164067543283302 1101 58-59 1.1732447046650947 1101 60-61 1.0842718314483228 1101 62-63 1.1317732664692635 1101 64-65 0.9138622563246201 1101 66-67 1.141978088797984 1101 68-69 1.0630906184991638 1101 70-71 1.3271129177152474 1101 72-73 1.0949830297626022 1101 74-75 1.066508963033975 1101 76-77 1.1256032881598266 1101 78-79 0.7227566270686836 1101 80-81 1.0116217634341744 1101 82-83 0.8622940428535344 1101 84-85 1.070364524987074 1101 86-87 1.2854771896304946 1101 88-89 1.6882400554669665 1101 90-91 1.3695821730093662 1101 92-93 1.3331639213488948 1101 94-95 1.2820630556469865 1101 96-97 1.4094679692726508 1101 98-99 1.3622509340404214 1101 100-101 1.0306632421988482 1102 1 0.40479729328172454 1102 2 0.5681519511577697 1102 3 0.45679939397520286 1102 4 0.5143412129944878 1102 5 0.3175951092933502 1102 6 0.3536001968115272 1102 7 0.40388786814001776 1102 8 0.38845055617557733 1102 9 0.5129573632887627 1102 10-11 0.4997209491729748 1102 12-13 0.5498240575006292 1102 14-15 0.7023642784013759 1102 16-17 0.5166412062440173 1102 18-19 0.5517698264084814 1102 20-21 0.5540797782912321 1102 22-23 0.5736165411505922 1102 24-25 0.46108649514994227 1102 26-27 0.5357528491357257 1102 28-29 0.33126773358313244 1102 30-31 0.4813244755518795 1102 32-33 0.6236856375813531 1102 34-35 0.5949037041530403 1102 36-37 0.6710238935398891 1102 38-39 0.5314943118945337 1102 40-41 0.7334901054274567 1102 42-43 0.8837257805808818 1102 44-45 0.8131038989296826 1102 46-47 0.7610377396155101 1102 48-49 0.7150569249068965 1102 50-51 1.0387527740108666 1102 52-53 1.051216067830211 1102 54-55 0.9464817451486169 1102 56-57 0.9084919353805603 1102 58-59 0.9256098262456405 1102 60-61 0.7608343314483257 1102 62-63 0.8263947520315718 1102 64-65 0.9238475336802452 1102 66-67 1.1833107255760993 1102 68-69 1.172413376857822 1102 70-71 1.0168640575328745 1102 72-73 1.212728565476887 1102 74-75 1.1963246925172584 1102 76-77 0.9193675358801912 1102 78-79 0.9623724135428517 1102 80-81 1.128198986078548 1102 82-83 1.2831954486286108 1102 84-85 0.8765907787864649 1102 86-87 0.7294171592353607 1102 88-89 1.0780885547070866 1102 90-91 1.1082861053801842 1102 92-93 1.053960843841299 1102 94-95 1.2164665510877128 1102 96-97 1.3275412025553273 1102 98-99 1.4516482554689922 1102 100-101 1.6679898949344114 1103 1 0.6159286678173537 1103 2 0.57529143106321 1103 3 0.32982427295752714 1103 4 0.49101125689857383 1103 5 0.5064839981822402 1103 6 0.5273455531100737 1103 7 0.546560388684874 1103 8 0.46780212659098197 1103 9 0.6476123221990377 1103 10-11 0.44203548255133995 1103 12-13 0.4878010922861691 1103 14-15 0.7929552949498344 1103 16-17 0.8481328194377582 1103 18-19 0.5517394312717059 1103 20-21 0.5534336001982396 1103 22-23 0.3601650045075644 1103 24-25 0.6077374013753172 1103 26-27 0.44111702790416274 1103 28-29 0.42962301895969546 1103 30-31 0.3961437933499212 1103 32-33 0.44022284348026375 1103 34-35 0.5025182487888671 1103 36-37 0.5079359727924029 1103 38-39 0.4256561941415242 1103 40-41 0.5731242380403145 1103 42-43 0.6172707541258475 1103 44-45 0.432848354631588 1103 46-47 0.5168195700781908 1103 48-49 0.7980221393614642 1103 50-51 0.811642563496406 1103 52-53 0.6805383688546343 1103 54-55 0.8204860229826778 1103 56-57 0.8817295803203322 1103 58-59 0.821554889850276 1103 60-61 0.7500935907075821 1103 62-63 0.7418253498025997 1103 64-65 0.775546284102397 1103 66-67 0.5718044776868751 1103 68-69 0.7838892296102742 1103 70-71 1.0739416214189497 1103 72-73 1.0351624279107483 1103 74-75 0.6923654445154526 1103 76-77 0.736911158530198 1103 78-79 0.8068596363279426 1103 80-81 0.49687060602676425 1103 82-83 0.5204306169276087 1103 84-85 0.595376099061145 1103 86-87 0.7065420044453106 1103 88-89 0.6108500091706688 1103 90-91 0.2292407378241812 1103 92-93 0.4448884583859325 1103 94-95 0.4682204630543936 1103 96-97 1.0201450526059865 1103 98-99 1.431701165521904 1103 100-101 1.2762708810877363 1104 1 0.4758420877307721 1104 2 0.554627650399425 1104 3 0.6597713629046176 1104 4 0.5329160188033342 1104 5 0.4236557153539593 1104 6 0.3751377609022839 1104 7 0.3591241212486125 1104 8 0.3184803372691931 1104 9 0.5469293015160162 1104 10-11 0.3208041213199806 1104 12-13 0.44047064495572386 1104 14-15 0.5236623656569037 1104 16-17 0.6315286828336184 1104 18-19 0.6836586231908939 1104 20-21 0.6636404304050743 1104 22-23 0.9570481213906774 1104 24-25 0.8537210473589596 1104 26-27 0.6655999523870904 1104 28-29 0.8463041156407982 1104 30-31 0.7790586562647874 1104 32-33 0.633633136890559 1104 34-35 0.6406038668744785 1104 36-37 0.9783448232012475 1104 38-39 1.0382487867341155 1104 40-41 1.0380785429946187 1104 42-43 0.9349523318074304 1104 44-45 1.1325982343814687 1104 46-47 1.2663722396308614 1104 48-49 1.2279211292604586 1104 50-51 1.09757810943195 1104 52-53 0.8244729527892289 1104 54-55 0.6537520162486743 1104 56-57 0.7106088491996019 1104 58-59 0.7110017392971244 1104 60-61 0.8715846921986845 1104 62-63 0.8985064464836903 1104 64-65 1.0346516232077363 1104 66-67 1.0147482006305957 1104 68-69 0.854538580259625 1104 70-71 1.0565005439778723 1104 72-73 1.1297559825043066 1104 74-75 0.7928753050253157 1104 76-77 0.8527745543935943 1104 78-79 0.9679755574438644 1104 80-81 0.9181885473447089 1104 82-83 1.0754185919155859 1104 84-85 1.0528748965599455 1104 86-87 1.124339022242328 1104 88-89 1.2499745882952489 1104 90-91 1.1319728205562605 1104 92-93 1.17039110388858 1104 94-95 1.1485764034103312 1104 96-97 1.4567972892582226 1104 98-99 1.2182427720635083 1104 100-101 0.5150010398178964 1105 1 0.3738604609966707 1105 2 0.3891968216022619 1105 3 0.47196686816943156 1105 4 0.5850926650393831 1105 5 0.41165451523394125 1105 6 0.37495202804328187 1105 7 0.4691922709207219 1105 8 0.3190232487031892 1105 9 0.6051208349550734 1105 10-11 0.40837002076706597 1105 12-13 0.6035798130153864 1105 14-15 0.5470932801769308 1105 16-17 0.4523750531849444 1105 18-19 0.5681184977497864 1105 20-21 0.4481331653928535 1105 22-23 0.4611771057176881 1105 24-25 0.6438786345462475 1105 26-27 0.6649998923810827 1105 28-29 0.7448296824831928 1105 30-31 0.4712278731864714 1105 32-33 0.5881142992925135 1105 34-35 0.7372492456980737 1105 36-37 0.5599386968743332 1105 38-39 0.4537760537465374 1105 40-41 0.4203524846745026 1105 42-43 0.11922790222160984 1105 44-45 0.2649757578481058 1105 46-47 0.2344119007398291 1105 48-49 0.4614230508811872 1105 50-51 0.2981195921516502 1105 52-53 0.26240960359716325 1105 54-55 0.39187582862991377 1105 56-57 0.5906897144289829 1105 58-59 0.621914259120544 1105 60-61 0.41465328477223196 1105 62-63 0.3800903191566789 1105 64-65 0.4446426223076472 1105 66-67 0.3463385025179271 1105 68-69 0.2718445965755407 1105 70-71 0.8623454141791775 1105 72-73 0.5783579855617518 1105 74-75 0.6298089983946511 1105 76-77 0.7477854840579887 1105 78-79 0.6878832625000868 1105 80-81 0.5921988055133802 1105 82-83 0.8474315074928569 1105 84-85 0.8213303135302148 1105 86-87 0.6658431726573717 1105 88-89 0.8603570551133579 1105 90-91 0.9168870262625468 1105 92-93 1.0550367113064638 1105 94-95 0.7067536497063891 1105 96-97 1.0289616485512951 1105 98-99 1.1705308580149634 1105 100-101 1.1908614830050723 1106 1 0.16212616345849895 1106 2 0.20373333944805339 1106 3 -0.012148570070948495 1106 4 0.24588841651182491 1106 5 0.27265930330190713 1106 6 0.04101808441456001 1106 7 0.27399527007125357 1106 8 0.28374126116064247 1106 9 0.4054101771152929 1106 10-11 0.3315309123606127 1106 12-13 0.3348608481904307 1106 14-15 0.41071405228063185 1106 16-17 0.21199608355779986 1106 18-19 -0.04178410653143061 1106 20-21 0.07021174781804262 1106 22-23 -0.008067678960493652 1106 24-25 0.40121761482699725 1106 26-27 0.352333808165163 1106 28-29 0.12448432820045952 1106 30-31 0.08819958666106942 1106 32-33 0.38536998777149023 1106 34-35 0.294325719597758 1106 36-37 0.34165182844834163 1106 38-39 0.5581410042012251 1106 40-41 0.537583476165743 1106 42-43 0.2684822509293596 1106 44-45 0.5648101023993348 1106 46-47 0.5881811170745479 1106 48-49 1.1404630967239697 1106 50-51 0.7715400644210106 1106 52-53 1.1191509329165612 1106 54-55 0.9436265101174683 1106 56-57 0.5887153678196668 1106 58-59 0.037995952881928474 1106 60-61 0.7854420347293534 1106 62-63 0.7844691919328888 1106 64-65 0.8578540983360341 1106 66-67 0.5954976144755868 1106 68-69 0.25868396728661125 1106 70-71 0.5707514697837226 1106 72-73 0.6852168475229448 1106 74-75 0.9644360315075815 1106 76-77 0.6279102603424214 1106 78-79 0.9908814041728213 1106 80-81 1.22379299203617 1106 82-83 1.2439029051930497 1106 84-85 1.174169357369383 1106 86-87 1.174751440700394 1106 88-89 1.1953916692330147 1106 90-91 1.5140264936227759 1106 92-93 1.4971238892518919 1106 94-95 1.083266693307472 1106 96-97 0.7156276964481201 1106 98-99 0.5360684714869279 1106 100-101 0.29120510204193195 1107 1 0.5764909528480082 1107 2 0.4956024823567624 1107 3 0.4224238442650403 1107 4 0.5429568229221999 1107 5 0.6103272761843357 1107 6 0.5694690763641503 1107 7 0.4543855220281827 1107 8 0.49856031411901824 1107 9 0.5795259855258905 1107 10-11 0.7080810435901768 1107 12-13 0.6173509651738627 1107 14-15 0.8426666752399541 1107 16-17 0.8434032446408892 1107 18-19 0.6640139938558249 1107 20-21 0.7550476772631995 1107 22-23 0.6969019568030603 1107 24-25 0.8257524554603535 1107 26-27 0.6785578334472504 1107 28-29 0.7807281483151556 1107 30-31 0.9261632340557711 1107 32-33 0.8892483157897217 1107 34-35 0.9383748860452314 1107 36-37 1.0129865684755615 1107 38-39 1.0864019196786359 1107 40-41 0.6631875449542264 1107 42-43 0.5367732714480198 1107 44-45 0.24397541709108594 1107 46-47 0.3253226357279644 1107 48-49 0.47347899594887366 1107 50-51 0.2668860707991314 1107 52-53 0.3970609749061822 1107 54-55 0.438089332887472 1107 56-57 0.34278137235462935 1107 58-59 0.40315301556684346 1107 60-61 0.2827051255263875 1107 62-63 0.302946928986092 1107 64-65 0.4864091523542271 1107 66-67 0.37936242594468794 1107 68-69 0.4620917120388661 1107 70-71 0.3615972716856355 1107 72-73 0.4398002824543923 1107 74-75 0.8048543533435293 1107 76-77 0.7716013534357344 1107 78-79 0.6940606731655876 1107 80-81 0.880913275883703 1107 82-83 0.9509864217229804 1107 84-85 1.0352724152966282 1107 86-87 0.9101659514070768 1107 88-89 1.0553024292893092 1107 90-91 1.0392711450820435 1107 92-93 1.1708931440945243 1107 94-95 1.0504765818919353 1107 96-97 1.3047121230411598 1107 98-99 1.0333152425868555 1107 100-101 0.7910697445541643 1108 1 0.1897155158899153 1108 2 0.20921433355754004 1108 3 0.18720900462797374 1108 4 0.5365441367171684 1108 5 0.3427651773205582 1108 6 0.2480711766928394 1108 7 0.22504867288744634 1108 8 -0.03641556048385297 1108 9 0.3570303116170308 1108 10-11 0.20614734952035008 1108 12-13 0.3995924755061253 1108 14-15 0.34365824279564094 1108 16-17 0.14311392299028824 1108 18-19 0.3250727646050322 1108 20-21 0.21521742483921003 1108 22-23 0.14039176187717572 1108 24-25 0.40667920031711446 1108 26-27 0.38835814657385725 1108 28-29 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-0.9911287496731447 2316 28-29 -1.0998732973837164 2316 30-31 -1.0603259104540186 2316 32-33 -0.7598874156286399 2316 34-35 -0.5943564066514213 2316 36-37 -0.8452008988559712 2316 38-39 -1.1233810433666207 2316 40-41 -0.9451697528382468 2316 42-43 -0.7605811979615495 2316 44-45 -1.1224586167511603 2316 46-47 -0.8949861734190279 2316 48-49 -0.8372297024668356 2316 50-51 -0.7676828512282015 2316 52-53 -0.6587499587151484 2316 54-55 -0.634256622233579 2316 56-57 -0.7373913538750259 2316 58-59 -0.6653082385013462 2316 60-61 -0.5317908376991731 2316 62-63 -0.48549986445089743 2316 64-65 -0.6559798022004131 2316 66-67 -0.2757041676296197 2316 68-69 -0.21818263928162196 2316 70-71 -0.7734156682116833 2316 72-73 -0.41466366340383587 2316 74-75 -0.21894012356954562 2316 76-77 -0.4736757206358426 2316 78-79 -0.6048218150828717 2316 80-81 -0.28757584312875295 2316 82-83 -0.05889379544145967 2316 84-85 -0.34237862453931456 2316 86-87 -0.47823221497031554 2316 88-89 -0.7628942899322233 2316 90-91 -0.7865483665711501 2316 92-93 -0.770605953481958 2316 94-95 -0.6897146845424622 2316 96-97 -0.46163212375846996 2316 98-99 -0.4726219939027452 2316 100-101 -0.38579798073755356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3330.0 3 721.0 4 557.0 5 563.0 6 658.0 7 729.0 8 988.0 9 1239.0 10 1699.0 11 2622.0 12 4046.0 13 5482.0 14 6807.0 15 9164.0 16 11146.0 17 13665.0 18 10942.0 19 7440.0 20 7238.0 21 7759.0 22 8370.0 23 9282.0 24 10322.0 25 11553.0 26 12961.0 27 14776.0 28 16974.0 29 19858.0 30 23088.0 31 27546.0 32 33307.0 33 40564.0 34 52232.0 35 69966.0 36 80273.0 37 96391.0 38 62269.0 39 5043.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85114174300498 22.104503815466984 15.833029138927035 19.211325302601008 2 14.603932824021099 23.783704805290057 41.31316915946122 20.299193211227628 3 17.0622400636505 32.12267187443492 30.721782214024806 20.09330584788977 4 10.840886239846073 19.504654712217988 40.33707783894045 29.317381208995492 5 10.397060206451053 41.46454380393661 34.30096715156864 13.837428838043705 6 25.293789120036113 41.22322185600065 18.89582748552396 14.587161538439277 7 24.511909876366413 36.871218268898346 23.3528178823897 15.264053972345536 8 25.068745000527976 34.90756129913225 21.43990580379938 18.5837878965404 9 27.96185632181414 17.24421761917368 22.169566355794533 32.62435970321765 10-11 22.509120735097977 30.023275595198484 27.806669347675765 19.660934322027767 12-13 25.220883708416146 28.725586347159176 28.30667395713091 17.746855987293763 14-15 22.484032808771957 29.01020410377246 28.691981291391567 19.813781796064017 16-17 18.042395006906727 32.35428976430343 29.793521418084772 19.809793810705063 18-19 17.899645164883328 29.457390132025278 34.85347320365626 17.78949149943513 20-21 19.215974191308373 28.801863318095016 34.94046930154505 17.04169318905156 22-23 19.191442822919438 28.69508546761258 34.785882509518906 17.327589199949077 24-25 18.37089190669355 29.555265688110826 34.92437281197707 17.14946959321855 26-27 17.733555065763824 29.67802743974995 35.056099799847665 17.532317694638564 28-29 17.678041484003913 28.961048260578043 35.80438274877636 17.55652750664168 30-31 17.98037360462492 29.789485714947077 34.88915123001337 17.340989450414636 32-33 17.343334647392197 30.287200222787224 34.66337790283439 17.706087226986188 34-35 17.998926572961214 29.667382284692522 34.90952543530215 17.42416570704411 36-37 18.207272174925677 29.41541955689931 34.17878816372882 18.198520104446196 38-39 17.561026761016638 30.095422676535566 34.52449567723343 17.819054885214367 40-41 17.52006612655599 29.718766881471907 35.0566928188614 17.704474173110704 42-43 17.505560971094518 29.755678843433714 34.08477552189243 18.653984663579326 44-45 17.344622147670417 30.022341440842176 33.90823379703271 18.724802614454696 46-47 18.044984475395633 30.017296159124662 33.1909822801139 18.7467370853658 48-49 17.81677721273508 30.446216620160914 32.46988574696869 19.267120420135317 50-51 18.224518009914565 30.28330836374995 32.18914314801854 19.303030478316956 52-53 17.964829116592036 30.495459570692812 32.441564432592756 19.0981468801224 54-55 17.897737954412015 30.474202383810223 32.2562031994308 19.371856462346962 56-57 18.12265910832815 30.69463926768955 31.408005668753887 19.774695955228413 58-59 17.887529763260513 29.79102630012936 32.310482235319384 20.010961701290746 60-61 18.266568618306263 30.604971398946194 31.61473935839062 19.51372062435692 62-63 18.47445164173083 30.25082370327739 32.23783388270986 19.03689077228192 64-65 18.395102968673402 29.7496388396014 32.30646647028796 19.548791721437237 66-67 18.098729941594378 30.117216138403542 31.7372889321416 20.04676498786048 68-69 18.274086349911496 29.994418028898995 32.06741170073693 19.664083920452573 70-71 18.556047948534005 29.522655219494133 31.91726158794807 20.00403524402379 72-73 18.07415099346943 29.644560884125244 31.656953852139118 20.624334270266207 74-75 17.986734718966083 30.024413208008344 31.574897784154267 20.41395428887131 76-77 17.796140780665436 29.392092692993504 32.53372803827115 20.278038488069914 78-79 17.80565786448369 28.948654655670808 32.34278046022388 20.902907019621622 80-81 17.857034400880377 29.9542459180678 31.46352326676047 20.725196414291354 82-83 18.34849052320142 28.960379216549946 31.570963557671313 21.120166702577325 84-85 18.056234400622383 28.679139420476794 31.599241685875167 21.66538449302565 86-87 18.000274757056918 29.032493637204997 31.732198634891258 21.235032970846827 88-89 18.321402897728998 28.239406163712864 32.017923858315896 21.42126708024224 90-91 18.27445971543072 28.369631960632148 31.8425256988206 21.513382625116535 92-93 18.5495627876302 28.399713056784094 31.683940146884844 21.366784008700858 94-95 18.18857695806942 27.47545768683907 32.182504841926814 22.15346051316469 96-97 18.638703620933807 27.296153417777912 31.766161706017726 22.298981255270554 98-99 18.517232925985304 27.31466315507066 31.69208162580531 22.47602229313872 100-101 18.808583641279366 26.779397234095686 32.28340927927961 22.12860984534534 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3519.0 1 2227.0 2 1286.0 3 1588.5 4 1274.0 5 1286.0 6 2138.0 7 4068.0 8 6115.0 9 6222.5 10 4533.5 11 3163.5 12 2847.5 13 2743.5 14 2643.0 15 2442.0 16 2292.0 17 2336.0 18 2379.5 19 2365.5 20 2482.0 21 2709.5 22 2952.5 23 3334.5 24 3609.0 25 3993.5 26 4784.0 27 5555.5 28 6289.5 29 7655.5 30 10051.5 31 12234.0 32 15560.5 33 19991.5 34 23187.5 35 26729.0 36 32804.5 37 36853.5 38 36339.0 39 35275.0 40 32714.0 41 29360.5 42 26280.5 43 24766.0 44 24569.0 45 23700.5 46 21363.0 47 18900.5 48 18938.0 49 17386.0 50 13847.0 51 11004.0 52 9083.5 53 8230.0 54 7948.5 55 7494.0 56 6780.0 57 6224.5 58 6004.0 59 5659.0 60 4883.0 61 4411.0 62 3928.0 63 2658.5 64 1556.0 65 1196.0 66 951.0 67 744.0 68 585.5 69 429.0 70 349.0 71 306.5 72 252.5 73 246.0 74 214.0 75 155.5 76 122.5 77 79.5 78 45.0 79 31.0 80 24.0 81 15.5 82 6.5 83 4.5 84 7.5 85 6.0 86 2.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17669939413219196 2 0.04497014040516506 3 0.04265656404991541 4 0.04844050493803953 5 0.052778460604132625 6 0.061888167502928124 7 0.03658342611738508 8 0.035715834984166465 9 0.04178897291669679 10-11 0.041644374394493686 12-13 0.03701722168399439 14-15 0.03332995936781526 16-17 0.03108868227366716 18-19 0.03802941133941611 20-21 0.04771751232702402 22-23 0.04540393597177437 24-25 0.06499703573029483 26-27 0.05010338794337522 28-29 0.04215046922220455 30-31 0.051766270948710905 32-33 0.047356016021516255 34-35 0.047139118238211604 36-37 0.04410254927194644 38-39 0.024943245080035283 40-41 0.024364850991222868 42-43 0.020822187197246843 44-45 0.004337955666093092 46-47 0.012580071431669967 48-49 0.011350983992943592 50-51 0.00896510837659239 52-53 0.008748210593287736 54-55 0.010411093598623421 56-57 0.008675911332186185 58-59 0.01033879433752187 60-61 0.010772589904131179 62-63 0.004121057882788438 64-65 0.007085327587952051 66-67 0.0039041600994837835 68-69 0.008386714287779979 70-71 0.0234249605969027 72-73 0.01872550862530185 74-75 0.020388391630637534 76-77 0.012001677342857557 78-79 0.005350145321514814 80-81 0.0067961305435458455 82-83 0.00563934236592102 84-85 0.005205546799311711 86-87 0.007229926110155154 88-89 0.02906430296282372 90-91 0.035354338678658705 92-93 0.020822187197246843 94-95 0.030799485229260955 96-97 0.033113061584510606 98-99 0.021183683502754604 100-101 0.12110126234509884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 691570.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.28536737538322 #Duplication Level Percentage of deduplicated Percentage of total 1 76.2212302037429 26.132728793389816 2 11.087190927736232 7.602568282369053 3 4.19743844034223 4.317321568880668 4 2.09933584124607 2.8790600224572276 5 1.2111298776595598 2.076201639743047 6 0.7951498481933071 1.6357202798272636 7 0.5451753391602383 1.3084075750975543 8 0.3979759413942932 1.0915801085813859 9 0.3113698554228458 0.9607886894513004 >10 2.435823023532478 17.164117933261366 >50 0.41094373819887353 9.819555597465278 >100 0.27198544319195417 16.618469246322075 >500 0.011014986795935839 2.404922519590206 >1k 0.0025419200298313475 1.3518520196790509 >5k 0.0012709600149156737 2.9416253467863047 >10k+ 4.2365333830522456E-4 1.6950803770983693 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11670 1.6874647541102132 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8219 1.1884552539873041 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6664 0.963604551961479 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5369 0.7763494657084604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2054 0.29700536460517374 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1789 0.2586867562213514 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1671 0.24162413060138527 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1528 0.2209465419263415 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1194 0.17265063551050508 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1071 0.1548650172795234 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 923 0.13346443599346414 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 914 0.1321630492936362 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 803 0.11611261332909177 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 757 0.10946108130774904 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 746 0.10787049756351491 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 721 0.10425553450843733 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.891970444062062E-4 0.0 18-19 0.0 0.0 0.0 2.891970444062062E-4 0.0 20-21 0.0 0.0 0.0 6.506933499139638E-4 0.0 22-23 0.0 0.0 0.0 0.002241277094148098 0.0 24-25 0.0 0.0 0.0 0.00795291872117067 0.0 26-27 0.0 0.0 0.0 0.016050435964544443 0.0 28-29 0.0 0.0 0.0 0.034775944589846286 0.0 30-31 0.0 0.0 0.0 0.08495163179432305 0.0 32-33 0.0 0.0 0.0 0.1607212574287491 0.0 34-35 7.229926110155155E-5 0.0 0.0 0.2588313547435545 0.0 36-37 1.445985222031031E-4 0.0 0.0 0.40292378211894675 0.0 38-39 1.445985222031031E-4 0.0 0.0 0.6107841577859074 0.0 40-41 1.445985222031031E-4 0.0 0.0 0.8552279595702532 0.0 42-43 1.445985222031031E-4 0.0 0.0 1.0818861431236173 0.0 44-45 1.445985222031031E-4 0.0 0.0 1.3373917318565005 0.0 46-47 1.445985222031031E-4 0.0 0.0 1.643940598927079 0.0 48-49 1.445985222031031E-4 0.0 0.0 1.9729022369391385 0.0 50-51 1.445985222031031E-4 0.0 0.0 2.321890770276328 0.0 52-53 1.445985222031031E-4 0.0 0.0 2.647960437844325 0.0 54-55 1.445985222031031E-4 0.0 0.0 2.9914542273377966 0.0 56-57 1.445985222031031E-4 0.0 0.0 3.3732666252150905 0.0 58-59 1.445985222031031E-4 0.0 0.0 3.793397631476206 0.0 60-61 1.445985222031031E-4 0.0 0.0 4.250039764593605 0.0 62-63 1.445985222031031E-4 0.0 0.0 4.6895469728299375 0.0 64-65 1.445985222031031E-4 0.0 0.0 5.13794699018176 0.0 66-67 1.445985222031031E-4 0.0 0.0 5.637317986610176 0.0 68-69 1.445985222031031E-4 0.0 0.0 6.156860476885926 0.0 70-71 1.445985222031031E-4 0.0 0.0 6.735832959787151 0.0 72-73 1.445985222031031E-4 0.0 0.0 7.30757551657822 0.0 74-75 1.445985222031031E-4 0.0 0.0 7.90165854504967 0.0 76-77 1.445985222031031E-4 0.0 0.0 8.483450699133854 0.0 78-79 1.445985222031031E-4 0.0 0.0 9.142096967768989 0.0 80-81 1.445985222031031E-4 0.0 0.0 9.852581806613937 0.0 82-83 1.445985222031031E-4 0.0 0.0 10.562777448414478 0.0 84-85 1.445985222031031E-4 0.0 0.0 11.283239585291437 0.0 86-87 1.445985222031031E-4 0.0 0.0 12.019896756655147 0.0 88-89 1.445985222031031E-4 0.0 0.0 12.758867504374106 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4335 0.0 42.081516 1 GTATCAA 7490 0.0 39.363213 1 GGTAAGG 140 1.0913936E-11 37.339706 3 CATGGGT 455 0.0 31.334023 4 GGTGTGC 205 1.8189894E-12 30.125807 8 AGGTAAG 160 1.893568E-9 29.702042 2 ATCAACG 9795 0.0 29.692945 3 GTACATG 6065 0.0 29.449787 1 TCAACGC 9935 0.0 29.322357 4 TATCAAC 9995 0.0 29.193884 2 ATAAGCC 115 3.8469407E-6 28.927208 3 CAACGCA 10090 0.0 28.914831 5 AACGCAG 10250 0.0 28.552057 6 GGATGAC 305 0.0 28.036373 8 CGTCACT 85 6.302922E-4 28.01772 1 TAAGGTG 240 0.0 27.717892 5 TACATGG 6375 0.0 27.507584 2 CATGGGA 3940 0.0 27.259539 4 ACATGGG 6230 0.0 27.156153 3 ATGGGAT 1540 0.0 27.152222 5 >>END_MODULE