FastQCFastQC Report
Thu 26 May 2016
SRR938606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691570
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105521.5258036062871438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80691.1667654756568386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68880.9959946209349739No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58450.8451783622771375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.30756105672600026No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.2487094581893373No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.23034544586954322No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.19361742122995504No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12470.18031435718726954No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.14821348525818065No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.14691209855835274No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA9580.13852538427057276No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8760.1266683054499183No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT7750.11206385470740488No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG7700.11134086209638938No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7110.10280954928640629No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC7080.10237575371979699No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT7050.10194195815318768RNA PCR Primer, Index 39 (95% over 23bp)
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT7010.10136356406437526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45450.041.2323881
GTACACG704.268173E-640.768931
CTGTGCG608.292495E-539.5791479
CATATAG1850.038.545673
CGATGTA500.001604760438.0510032
GTATCAA75150.036.9623831
GTCTGGA550.002562007934.591821
TAAGGTG2700.033.4538355
GTATGGT1001.2846631E-633.294631
GGTACTC1900.032.5271343
GTAAGGT2500.032.326974
GGGACTC2350.030.315947
GTGGTAC2250.029.5952241
GGTATAT1802.0736479E-1029.0667381
GTACATG64150.028.4715231
ATCAACG97250.028.4015873
TCAACGC97650.028.333934
AGGTAAG1703.5852281E-927.9786782
CAACGCA98900.027.9758155
GGTAAGG1703.603418E-927.9645043
Produced by FastQC (version 0.11.5)