##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938606_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 691570 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.728563992076 31.0 31.0 34.0 30.0 34.0 2 31.92932602628801 31.0 31.0 34.0 30.0 34.0 3 31.956081090851253 31.0 31.0 34.0 30.0 34.0 4 35.67766531226051 37.0 35.0 37.0 33.0 37.0 5 35.568009022947784 37.0 35.0 37.0 33.0 37.0 6 35.618914932689385 37.0 35.0 37.0 33.0 37.0 7 35.55415937649117 37.0 35.0 37.0 33.0 37.0 8 35.56475700218344 37.0 35.0 37.0 33.0 37.0 9 37.09145711930824 39.0 37.0 39.0 33.0 39.0 10-11 37.00528507598652 39.0 37.0 39.0 33.0 39.0 12-13 36.91089043769973 39.0 37.0 39.0 32.5 39.0 14-15 37.794756857584915 40.0 37.0 41.0 32.0 41.0 16-17 37.76897711005394 39.0 37.0 41.0 32.5 41.0 18-19 38.00112642248796 39.5 37.0 41.0 33.0 41.0 20-21 38.0960813800483 40.0 37.0 41.0 33.0 41.0 22-23 37.95871061497752 40.0 37.0 41.0 33.0 41.0 24-25 37.825974232543345 40.0 37.0 41.0 33.0 41.0 26-27 37.588466821869076 40.0 36.0 41.0 32.0 41.0 28-29 37.424424859377936 39.0 36.0 41.0 32.0 41.0 30-31 37.070350073022254 39.0 35.5 40.5 31.5 41.0 32-33 36.69848171551686 38.5 35.0 40.0 31.0 41.0 34-35 36.425606952296945 38.0 35.0 40.0 30.0 41.0 36-37 36.26868212906864 38.0 35.0 40.0 30.0 41.0 38-39 36.06376577931374 38.0 35.0 40.0 30.0 41.0 40-41 35.80141706551759 38.0 35.0 40.0 30.0 41.0 42-43 35.46279913819281 38.0 35.0 40.0 28.5 41.0 44-45 35.01212603207195 38.0 34.5 40.0 26.0 41.0 46-47 34.415437338230404 38.0 34.0 40.0 23.5 41.0 48-49 33.89301878334803 38.0 33.0 40.0 21.0 41.0 50-51 33.50218488367049 38.0 33.0 40.0 18.0 41.0 52-53 33.04611029975274 37.0 33.0 40.0 14.0 41.0 54-55 32.77144106887228 37.0 33.0 40.0 9.5 41.0 56-57 32.462493312318344 37.0 32.0 40.0 8.0 41.0 58-59 32.06075017713319 36.0 31.0 40.0 4.0 41.0 60-61 31.701759041022598 36.0 31.0 39.0 2.0 41.0 62-63 31.25104038636725 35.5 30.5 39.0 2.0 40.0 64-65 30.763097011148545 35.0 29.5 39.0 2.0 40.0 66-67 30.290151394652746 35.0 29.0 38.0 2.0 40.0 68-69 29.781160981534768 35.0 28.0 38.0 2.0 40.0 70-71 29.244136529924667 34.0 27.5 37.0 2.0 39.5 72-73 28.839336581980135 34.0 27.0 37.0 2.0 39.0 74-75 28.369232326445623 34.0 26.0 36.0 2.0 39.0 76-77 27.06810373497983 31.5 25.5 35.0 2.0 37.0 78-79 27.73912836010816 33.0 26.0 35.0 2.0 37.5 80-81 27.453142125887474 33.0 26.0 35.0 2.0 37.0 82-83 27.00754659687378 33.0 25.5 35.0 2.0 36.5 84-85 26.46708576138352 33.0 24.5 35.0 2.0 36.0 86-87 25.955470162094944 32.5 23.0 35.0 2.0 36.0 88-89 25.363929898636435 32.0 20.0 35.0 2.0 35.0 90-91 24.856424512341484 31.5 18.0 34.0 2.0 35.0 92-93 24.210198533770985 31.0 9.5 34.0 2.0 35.0 94-95 23.540507107017365 31.0 2.0 34.0 2.0 35.0 96-97 22.66233786890698 30.5 2.0 34.0 2.0 35.0 98-99 21.603361192648613 30.0 2.0 34.0 2.0 35.0 100-101 18.221931257862543 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3016888245955691 1101 2 0.33045148343662945 1101 3 0.27419152076279474 1101 4 0.15200401333370195 1101 5 0.1870628050839116 1101 6 0.08613194217062414 1101 7 0.18202596273164318 1101 8 0.17671541891292009 1101 9 0.4155381453615661 1101 10-11 0.26646860155580043 1101 12-13 0.33226870408160636 1101 14-15 0.370337887818323 1101 16-17 0.3056652897018708 1101 18-19 0.5757090581430973 1101 20-21 0.4480004199234173 1101 22-23 0.40335555686630187 1101 24-25 0.40410909416089424 1101 26-27 0.6014523108864793 1101 28-29 0.5984608195339192 1101 30-31 0.5420350275411678 1101 32-33 0.3930540615468061 1101 34-35 0.44378473415937236 1101 36-37 0.4467292252715822 1101 38-39 0.1401635452393748 1101 40-41 0.44736601416828137 1101 42-43 0.5259217178817366 1101 44-45 0.4987097045480766 1101 46-47 0.40015934412404874 1101 48-49 0.35058554486684557 1101 50-51 0.5238824120209884 1101 52-53 0.7985672917941429 1101 54-55 0.5848755748609662 1101 56-57 0.5097363420708021 1101 58-59 0.8817468103907302 1101 60-61 0.7396818998697547 1101 62-63 0.6922764752989501 1101 64-65 0.6192081770570041 1101 66-67 0.848363139878547 1101 68-69 0.5118847716597976 1101 70-71 0.4916778051711539 1101 72-73 0.14192248476905434 1101 74-75 0.47069376218421155 1101 76-77 0.45895283168487566 1101 78-79 0.7206413229942861 1101 80-81 0.5416835241757276 1101 82-83 0.777096798328305 1101 84-85 0.5778677158950707 1101 86-87 0.4778515188288921 1101 88-89 0.23417441471860911 1101 90-91 0.32291580511291684 1101 92-93 0.36187198324456915 1101 94-95 0.1678805381616293 1101 96-97 0.31613924771863466 1101 98-99 0.362061104908701 1101 100-101 0.2024065801849524 1102 1 0.4117762106138052 1102 2 0.3950838922512183 1102 3 0.37264326848316287 1102 4 0.375166057406652 1102 5 0.25287302544865753 1102 6 0.357256562231413 1102 7 0.4138316238258639 1102 8 0.4723081240800937 1102 9 0.6265278870029078 1102 10-11 0.6052152770877157 1102 12-13 0.6839214396439175 1102 14-15 0.6489639326511494 1102 16-17 0.8427212357505027 1102 18-19 0.7687965391461375 1102 20-21 0.8559079047258464 1102 22-23 0.8416605530669159 1102 24-25 0.6604873236441762 1102 26-27 0.8068094537436181 1102 28-29 0.6748570961296565 1102 30-31 0.7101984963861554 1102 32-33 0.8934221276562297 1102 34-35 0.8713350761046641 1102 36-37 0.9273143316545642 1102 38-39 0.9755358856649039 1102 40-41 1.2293877656880383 1102 42-43 1.1646470218331046 1102 44-45 1.2053752630587127 1102 46-47 1.0337934626650735 1102 48-49 1.2366892682710926 1102 50-51 1.4068516369449995 1102 52-53 1.3630216041041763 1102 54-55 1.230081216958233 1102 56-57 1.1924158633473994 1102 58-59 1.5603894775639802 1102 60-61 1.6755172912071465 1102 62-63 1.6180624745390766 1102 64-65 1.6074514331360348 1102 66-67 1.391799690030524 1102 68-69 1.5630435862494672 1102 70-71 1.428040362162033 1102 72-73 1.552812492367842 1102 74-75 1.4887432492662747 1102 76-77 1.5891936181590438 1102 78-79 1.8678107758818214 1102 80-81 1.9467842080663011 1102 82-83 2.0018284662614363 1102 84-85 1.7201715722780513 1102 86-87 1.8142948129018386 1102 88-89 1.8991273972414078 1102 90-91 1.4534201744138286 1102 92-93 1.6528009346123476 1102 94-95 1.6769539614139042 1102 96-97 1.5732132408797348 1102 98-99 1.3686530501974552 1102 100-101 1.3136884000937634 1103 1 0.17414252829927435 1103 2 0.11312509454773689 1103 3 0.1361128170590895 1103 4 0.10833503185222071 1103 5 0.07797715693576635 1103 6 0.02316897920766081 1103 7 0.23655142569460708 1103 8 4.875224625067176E-5 1103 9 0.24394092313934834 1103 10-11 0.157689666370608 1103 12-13 0.09601870408161517 1103 14-15 0.3604825637442488 1103 16-17 0.44396968785002144 1103 18-19 0.24857364147642613 1103 20-21 0.32484069770119817 1103 22-23 0.37398634390333996 1103 24-25 0.3228243719386725 1103 26-27 0.5050634219975905 1103 28-29 0.3230210047191022 1103 30-31 0.2908255368004262 1103 32-33 0.5716941078431006 1103 34-35 0.5097916786038184 1103 36-37 0.4751551511975123 1103 38-39 0.712848730424561 1103 40-41 0.6516426345386463 1103 42-43 0.5059911623261826 1103 44-45 0.6386691952888128 1103 46-47 0.7033769367166371 1103 48-49 0.592599433755737 1103 50-51 0.5202018564654267 1103 52-53 0.6610267825348899 1103 54-55 0.8144299730091191 1103 56-57 0.6695395828115451 1103 58-59 0.511388014094436 1103 60-61 0.6700349091290185 1103 62-63 0.671194299373024 1103 64-65 0.5983227603903387 1103 66-67 0.7369511028415126 1103 68-69 0.7216532901783204 1103 70-71 0.5642646107267062 1103 72-73 0.3666794292135016 1103 74-75 0.49828056773976215 1103 76-77 0.4670141742774696 1103 78-79 0.43494687854984093 1103 80-81 0.5062900056572062 1103 82-83 0.14080628906904735 1103 84-85 0.05616053996914516 1103 86-87 0.2070934169770382 1103 88-89 0.5707889980519454 1103 90-91 0.7020940458536593 1103 92-93 0.6054599462075316 1103 94-95 0.7266016029764408 1103 96-97 0.7588070717927131 1103 98-99 0.5482185123161081 1103 100-101 -0.03555059574097541 1104 1 0.2805398346965795 1104 2 0.3114512128738589 1104 3 0.29089877184504687 1104 4 0.15685836037554424 1104 5 0.12742400638261842 1104 6 0.30243785847653726 1104 7 0.4162724454156219 1104 8 0.44691743911494086 1104 9 0.522324761523187 1104 10-11 0.39990226858321165 1104 12-13 0.4742871023500115 1104 14-15 0.5618005050621946 1104 16-17 0.6415069653873005 1104 18-19 0.6741724870752748 1104 20-21 0.5984626313231232 1104 22-23 0.6805279504449473 1104 24-25 0.5662009953152918 1104 26-27 0.5907199876541611 1104 28-29 0.6826554443535358 1104 30-31 0.6907870567619483 1104 32-33 0.6972737134227103 1104 34-35 0.8483775371896769 1104 36-37 0.773442789485145 1104 38-39 0.9752825928584272 1104 40-41 1.1081986710946907 1104 42-43 0.8907530670880845 1104 44-45 0.8269327835524081 1104 46-47 0.8728911262308259 1104 48-49 0.792743733900032 1104 50-51 1.0628569791205535 1104 52-53 1.0555241370322435 1104 54-55 1.1476382462173902 1104 56-57 1.127442420714381 1104 58-59 1.1451734878799087 1104 60-61 0.8980772371713392 1104 62-63 0.8826613508400776 1104 64-65 0.9650471471147242 1104 66-67 0.7414051672955786 1104 68-69 0.5526585811836107 1104 70-71 0.6873814938435885 1104 72-73 0.41908924162331473 1104 74-75 0.19652010597930314 1104 76-77 0.050063717327013535 1104 78-79 0.11168569528865646 1104 80-81 0.12778110714830504 1104 82-83 0.36566439392714756 1104 84-85 0.42127838508699256 1104 86-87 0.6381949080785319 1104 88-89 0.7352623025252498 1104 90-91 0.6776303613899692 1104 92-93 1.092540273287856 1104 94-95 1.1889585053333427 1104 96-97 1.1515728245584675 1104 98-99 1.2798010038986014 1104 100-101 1.5712411310507512 1105 1 0.14172595330844118 1105 2 0.2687183681608154 1105 3 0.20954777960296767 1105 4 0.12311212860951315 1105 5 0.12343789348561529 1105 6 0.19094099450442314 1105 7 0.17333386584338228 1105 8 0.12402800731462094 1105 9 0.341806709718 1105 10-11 0.1603854597594747 1105 12-13 0.3143496800363508 1105 14-15 0.5184604567716491 1105 16-17 0.3211963628984762 1105 18-19 0.3368244665589373 1105 20-21 0.41110818159243934 1105 22-23 0.217288769383984 1105 24-25 0.2626420679939869 1105 26-27 0.29231157538435326 1105 28-29 0.18301262292853693 1105 30-31 0.3684262492526216 1105 32-33 0.18436527972220063 1105 34-35 0.2879214915851165 1105 36-37 0.4518034826973292 1105 38-39 0.2254004617881762 1105 40-41 0.4077786290904868 1105 42-43 0.6341916966653329 1105 44-45 0.577843588918654 1105 46-47 0.42317472601937567 1105 48-49 0.317996259152558 1105 50-51 0.16478145728265048 1105 52-53 0.20144587206289088 1105 54-55 0.4235944132626699 1105 56-57 0.2291383045884814 1105 58-59 0.20601528634546895 1105 60-61 0.24429757349069092 1105 62-63 0.2564130117204506 1105 64-65 0.5660643916963295 1105 66-67 0.36321020494219525 1105 68-69 0.4814657476145392 1105 70-71 0.3463045290042466 1105 72-73 0.6247526651085167 1105 74-75 0.4351153940795385 1105 76-77 0.6421443539621023 1105 78-79 0.7103955662757535 1105 80-81 0.8984153134260815 1105 82-83 0.5900796925998755 1105 84-85 0.6046324347776739 1105 86-87 0.5993695969759898 1105 88-89 0.7346550989477478 1105 90-91 0.968595172156764 1105 92-93 0.9679849606137303 1105 94-95 0.9930031955166463 1105 96-97 1.1337853315234447 1105 98-99 1.298067116224118 1105 100-101 0.2979566597464789 1106 1 0.10068133529457057 1106 2 0.2061223999844195 1106 3 0.06406759066294043 1106 4 0.034495543290901765 1106 5 0.01813671735209965 1106 6 -0.11886092516176205 1106 7 0.21818190067743615 1106 8 0.12571684544644057 1106 9 0.17149838073959955 1106 10-11 0.1340874050508063 1106 12-13 -0.12726232302252782 1106 14-15 0.16602596734756503 1106 16-17 -0.04880042891439018 1106 18-19 0.33047568617448064 1106 20-21 0.2543953022343999 1106 22-23 -0.053635571878345445 1106 24-25 0.03951164408956487 1106 26-27 0.05089952914611473 1106 28-29 0.22901984235844708 1106 30-31 0.290310928040455 1106 32-33 0.29111821097619384 1106 34-35 0.2258037248869087 1106 36-37 -0.1859205607483858 1106 38-39 0.053198495310702754 1106 40-41 0.20911819854774194 1106 42-43 0.3175809546862993 1106 44-45 0.3160454302970024 1106 46-47 0.21132642686298908 1106 48-49 0.19429542361149288 1106 50-51 0.1690812351308253 1106 52-53 0.3561490945758905 1106 54-55 0.21130322286382608 1106 56-57 0.015275304267660772 1106 58-59 0.2116215429156938 1106 60-61 -0.44457385084350776 1106 62-63 -0.23432057355982394 1106 64-65 -0.35843425339948354 1106 66-67 0.35878129251763724 1106 68-69 -0.7982347005227943 1106 70-71 -0.707781672360948 1106 72-73 -0.13448398812680296 1106 74-75 0.08418007637821745 1106 76-77 0.25464982704864525 1106 78-79 0.05679503198144431 1106 80-81 -0.44377920763597345 1106 82-83 -0.09334386679294937 1106 84-85 0.07840600940434328 1106 86-87 0.1587170234651225 1106 88-89 0.35708476600248673 1106 90-91 0.23119638642532792 1106 92-93 0.08423735414328348 1106 94-95 0.7007454186894435 1106 96-97 0.75126652125644 1106 98-99 0.5427226598302433 1106 100-101 0.15125978096954285 1107 1 0.18699333334388513 1107 2 0.157384104567182 1107 3 0.37090669572915047 1107 4 0.16445985115335304 1107 5 0.05735006955228528 1107 6 -0.006869403724401479 1107 7 -0.002481523809407804 1107 8 -0.010700462648316034 1107 9 0.3153152314988503 1107 10-11 0.3213674491331844 1107 12-13 0.1682125129645229 1107 14-15 0.3331838248977306 1107 16-17 0.34771925168033846 1107 18-19 0.23750141177701778 1107 20-21 0.27266184320740194 1107 22-23 0.2691161978488097 1107 24-25 0.5358406722227969 1107 26-27 0.20533858275727113 1107 28-29 0.15002962841682432 1107 30-31 0.22044918467440766 1107 32-33 0.27251759620359906 1107 34-35 0.311161500646584 1107 36-37 0.2233611229835617 1107 38-39 0.23991118992309168 1107 40-41 0.5054445387308633 1107 42-43 0.4116678484335523 1107 44-45 0.5342676200931606 1107 46-47 0.49859053524113506 1107 48-49 0.5889056996447692 1107 50-51 0.43451918860241534 1107 52-53 0.4199091911884949 1107 54-55 0.3379911115366099 1107 56-57 0.6975202418016195 1107 58-59 0.21600194161549524 1107 60-61 0.4736289473125588 1107 62-63 0.42939478788307284 1107 64-65 0.5034012709331854 1107 66-67 0.6305426486268679 1107 68-69 0.5298104109599358 1107 70-71 0.7850482207162379 1107 72-73 0.7355862633693278 1107 74-75 0.6094544872851486 1107 76-77 0.8175171402313097 1107 78-79 0.5819216056322354 1107 80-81 0.8765716466521702 1107 82-83 0.7683831795530693 1107 84-85 0.6326382828533497 1107 86-87 0.7089048919782854 1107 88-89 0.522758491098152 1107 90-91 0.42619852718559414 1107 92-93 0.5138025014141476 1107 94-95 0.22481505195704798 1107 96-97 0.42836691427314477 1107 98-99 0.030205452149616008 1107 100-101 -0.1973231893305254 1108 1 0.05003066133026124 1108 2 0.12010922153186598 1108 3 0.015326724844427986 1108 4 0.07036073102077722 1108 5 0.022043672430854144 1108 6 -0.06828982653686211 1108 7 -0.09838432638400718 1108 8 0.13828004476326328 1108 9 -0.08839467776768117 1108 10-11 -0.056580930757128556 1108 12-13 0.11683503061222922 1108 14-15 -0.0034025458551454335 1108 16-17 -0.20050499091037466 1108 18-19 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2316 66-67 -0.19467221194824447 2316 68-69 -0.19055940966631724 2316 70-71 -0.37307714239312517 2316 72-73 -0.1489964090063154 2316 74-75 0.18797535047919922 2316 76-77 -0.18842009625829093 2316 78-79 0.09324788591715105 2316 80-81 -0.08036062332089244 2316 82-83 -0.1612605680451722 2316 84-85 0.10831109377058112 2316 86-87 0.46308599954607743 2316 88-89 0.5480645957064283 2316 90-91 0.4589717083605471 2316 92-93 0.12320063683049298 2316 94-95 -0.23727951765704702 2316 96-97 0.1109305619946852 2316 98-99 0.03101408190464383 2316 100-101 0.08363649391973027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 22.0 5 69.0 6 148.0 7 340.0 8 599.0 9 985.0 10 1649.0 11 2633.0 12 3737.0 13 5057.0 14 6336.0 15 8490.0 16 10440.0 17 13331.0 18 11460.0 19 7659.0 20 6830.0 21 7080.0 22 7784.0 23 8538.0 24 9590.0 25 10416.0 26 11963.0 27 13800.0 28 16696.0 29 19592.0 30 22843.0 31 27214.0 32 32527.0 33 40159.0 34 52227.0 35 69797.0 36 85326.0 37 107911.0 38 64846.0 39 3467.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.16472396585705 23.051695940933797 16.468926186045234 19.31465390716392 2 14.681629192839516 24.398976908098224 40.39259994386883 20.526793955193433 3 16.966928537241003 32.020076262032525 31.204387741970002 19.80860745875647 4 10.946975721908123 20.311609815347687 39.58644822649913 29.154966236245066 5 10.394082110571091 41.759127658404275 34.38651540984366 13.460274821180974 6 25.26179562444872 41.67416169006753 18.512804199141083 14.551238486342669 7 24.21634830892028 36.900964472143094 23.087901441647265 15.794785777289356 8 25.155804907673847 35.47507844469829 21.21853174660555 18.150584901022313 9 27.191752100293538 17.339387191462905 22.108969446332257 33.3598912619113 10-11 22.364547334326243 30.292956605983484 27.725176048700785 19.617320010989488 12-13 24.873910088638894 28.818846971383955 28.30704050204607 18.000202437931083 14-15 22.19189669881574 29.28799687667192 28.328224185548823 20.19188223896352 16-17 17.907948580765503 32.62937952774123 29.626068221582774 19.836603669910495 18-19 17.750119293780816 29.675376317654035 34.58941249620429 17.98509189236086 20-21 18.971253813786024 29.03957661552699 34.63315354917073 17.35601602151626 22-23 18.931633818702373 28.96734965368654 34.502147288054715 17.59886923955637 24-25 18.190908197024733 29.895686324410615 34.70280579783911 17.21059968072554 26-27 17.646076335966175 29.971280740164445 34.821741029208034 17.56090189466134 28-29 17.41421692670301 29.263993521986205 35.61129025261362 17.710499298697165 30-31 17.729441705105774 29.94801683126798 34.83392859724974 17.488612866376503 32-33 17.246699538730713 30.392657287042525 34.55051549373165 17.810127680495107 34-35 17.739815271416816 30.002198116850153 34.85548104774975 17.40250556398328 36-37 18.034222206477015 29.71245798500076 33.93987725004826 18.313442558473962 38-39 17.453056302070795 30.219732151055638 34.43123492057295 17.895976626300616 40-41 17.417842548059543 29.835092214744524 34.96047939520315 17.786585841992775 42-43 17.424835608960805 29.84264008936221 34.04801411286679 18.6845101888102 44-45 17.187992538716255 30.146839799297254 33.765490116691005 18.899677545295486 46-47 18.016108275373426 30.119582977861963 32.88228234307445 18.982026403690156 48-49 17.778099107827117 30.449773703312754 32.25204968405223 19.5200775048079 50-51 18.055728270457074 30.31818904810793 32.0921237184956 19.533958962939398 52-53 17.896959093078067 30.705929985395546 32.0690602542042 19.32805066732218 54-55 17.856760703905607 30.652211634397098 31.871972468441374 19.619055193255925 56-57 18.031435718726954 30.852625186170595 30.962664661567157 20.15327443353529 58-59 17.603207195222463 30.207498879361456 31.93610191303845 20.25319201237763 60-61 18.184276356695634 31.1228075249071 31.01905808522637 19.6738580331709 62-63 18.24818890350941 30.89282357534306 31.64690486863224 19.212082652515292 64-65 18.064476481050367 30.408490825223765 31.551903639544804 19.975129054181064 66-67 17.799104499409676 30.686692641437546 30.98789201648138 20.526310842671407 68-69 18.098581111613193 30.509751468594672 31.2 20.191667419792136 70-71 18.281282534761587 30.058070934456193 31.077276565717327 20.583369965064897 72-73 17.769567795017135 30.084734734011022 30.77909683763032 21.366600633341527 74-75 17.544138698902497 30.447604725479703 30.611434851135822 21.396821724481978 76-77 17.206645748080454 29.825541882961954 31.892650057116416 21.075162311841172 78-79 17.2087424266524 29.398253249851784 31.649218444987493 21.74378587850832 80-81 17.19454363652007 30.342725390946114 30.78549278840071 21.677238184133106 82-83 17.51913055046243 29.495607058782703 30.83792678624104 22.147335604513827 84-85 17.087061770098853 29.17594558169398 30.9145491459542 22.822443502252966 86-87 16.927298163927077 29.56876326263543 30.976310718920697 22.527627854516798 88-89 17.16449527885825 28.946599765750392 30.9786427982706 22.910262157120755 90-91 17.0188721807773 28.774761512955738 31.134904785936456 23.071461520330505 92-93 17.11584372166736 28.991802603096474 30.71045070499927 23.181902970236898 94-95 16.68124701765548 27.95624448718134 31.239860028630506 24.122648466532674 96-97 16.96567231082898 28.021819917000446 30.794351981722745 24.21815579044782 98-99 16.592270619417583 28.095244292326576 30.838643721739984 24.47384136651585 100-101 16.515175614905218 27.75713232210767 31.267189149326896 24.460502913660225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3780.0 1 2351.5 2 1187.0 3 1460.5 4 1310.0 5 1342.5 6 2214.0 7 4049.5 8 5646.5 9 5702.5 10 4423.5 11 3215.0 12 2815.5 13 2673.0 14 2556.0 15 2398.0 16 2259.0 17 2147.0 18 2152.5 19 2213.5 20 2311.5 21 2467.5 22 2704.0 23 3014.5 24 3254.5 25 3616.0 26 4341.5 27 5252.5 28 6051.5 29 7493.5 30 9900.5 31 11959.5 32 15500.0 33 19916.5 34 23081.0 35 26689.5 36 32991.0 37 37163.0 38 36827.0 39 35791.0 40 33189.0 41 29738.5 42 26337.5 43 25350.0 44 25466.5 45 24474.5 46 22104.5 47 19608.0 48 19681.0 49 17894.5 50 14387.5 51 11433.0 52 8967.0 53 8004.0 54 7734.5 55 7431.5 56 6775.0 57 6154.0 58 5857.0 59 5581.0 60 4752.5 61 4214.0 62 3722.0 63 2340.0 64 1314.0 65 1040.5 66 817.5 67 609.0 68 470.0 69 336.0 70 273.0 71 255.5 72 208.5 73 193.5 74 179.5 75 130.0 76 81.5 77 48.0 78 30.5 79 20.0 80 10.5 81 5.0 82 3.0 83 3.0 84 3.0 85 2.0 86 2.5 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010121896554217216 2 0.04800670937143023 3 0.0010121896554217216 4 0.0 5 0.0936998423876108 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.891970444062062E-4 26-27 0.0069407290657489485 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009977298032014113 36-37 2.1689778330465464E-4 38-39 0.0033980652717729228 40-41 0.004627152710499299 42-43 3.614963055077577E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.1689778330465464E-4 68-69 0.001084488916523273 70-71 2.891970444062062E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0015182844831325825 82-83 8.675911332186186E-4 84-85 0.005494743843717917 86-87 0.0018074815275387886 88-89 0.0 90-91 0.0033257660106713713 92-93 0.0013013866998279277 94-95 0.0 96-97 0.0 98-99 1.445985222031031E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 691570.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.73416800349044 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8413012468287 24.498677286135294 2 11.69409357004241 7.655928471406111 3 4.359702334783931 4.281336860160801 4 2.230280073678317 2.9202545050649222 5 1.3231469110943597 2.1656056640531096 6 0.8377294453961481 1.6453425844241032 7 0.603843510407876 1.3836420442256188 8 0.44693653213318635 1.17040764237961 9 0.32686140355796073 0.9629582488130678 >10 2.5907947780082865 17.58052500499527 >50 0.4352434605193603 9.962563123803593 >100 0.2919315893618196 17.039317454118716 >500 0.01326961769826453 2.736095969090534 >1k 0.0030962441295950566 1.4576005779136587 >5k 0.001326961769826453 3.011920852464481 >10k+ 4.4232058994215096E-4 1.5278237109511201 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10552 1.5258036062871438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8069 1.1667654756568386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6888 0.9959946209349739 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5845 0.8451783622771375 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2127 0.30756105672600026 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1720 0.2487094581893373 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1593 0.23034544586954322 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1339 0.19361742122995504 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1247 0.18031435718726954 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1025 0.14821348525818065 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1016 0.14691209855835274 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 958 0.13852538427057276 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 876 0.1266683054499183 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 775 0.11206385470740488 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 770 0.11134086209638938 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 711 0.10280954928640629 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 708 0.10237575371979699 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 705 0.10194195815318768 RNA PCR Primer, Index 39 (95% over 23bp) CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 701 0.10136356406437526 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.891970444062062E-4 0.0 18-19 0.0 0.0 0.0 2.891970444062062E-4 0.0 20-21 0.0 0.0 0.0 6.506933499139638E-4 0.0 22-23 0.0 0.0 0.0 0.0023135763552496495 0.0 24-25 0.0 0.0 0.0 0.008097517243373773 0.0 26-27 0.0 0.0 0.0 0.01648423153115375 0.0 28-29 0.0 0.0 0.0 0.03528203941755716 0.0 30-31 0.0 0.0 0.0 0.08581922292754168 0.0 32-33 0.0 0.0 0.0 0.1623118411729832 0.0 34-35 0.0 0.0 0.0 0.2614341281432104 0.0 36-37 0.0 0.0 0.0 0.4070448400017352 0.0 38-39 0.0 0.0 0.0 0.6155559090186098 0.0 40-41 0.0 0.0 0.0 0.8609396011972758 0.0 42-43 0.0 0.0 0.0 1.089694463322585 0.0 44-45 0.0 0.0 0.0 1.3473690298885146 0.0 46-47 0.0 0.0 0.0 1.6535564006535854 0.0 48-49 0.0 0.0 0.0 1.983602527582168 0.0 50-51 0.0 0.0 0.0 2.3324464623971544 0.0 52-53 0.0 0.0 0.0 2.6608297063204014 0.0 54-55 0.0 0.0 0.0 3.0055525832525993 0.0 56-57 0.0 0.0 0.0 3.3885217693075176 0.0 58-59 0.0 0.0 0.0 3.808291279263126 0.0 60-61 0.0 0.0 0.0 4.263053631591886 0.0 62-63 0.0 0.0 0.0 4.702343942044912 0.0 64-65 0.0 0.0 0.0 5.152117645357665 0.0 66-67 0.0 0.0 0.0 5.651777838830487 0.0 68-69 0.0 0.0 0.0 6.168789854967682 0.0 70-71 0.0 0.0 0.0 6.749425220874242 0.0 72-73 0.0 0.0 0.0 7.322902959931749 0.0 74-75 0.0 0.0 0.0 7.919877958847261 0.0 76-77 0.0 0.0 0.0 8.50738175455847 0.0 78-79 0.0 0.0 0.0 9.17289645299825 0.0 80-81 0.0 0.0 0.0 9.891478809086571 0.0 82-83 0.0 0.0 0.0 10.608036785864048 0.0 84-85 0.0 0.0 0.0 11.340717497867171 0.0 86-87 0.0 0.0 0.0 12.08619517908527 0.0 88-89 0.0 0.0 0.0 12.838252093063609 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4545 0.0 41.232388 1 GTACACG 70 4.268173E-6 40.76893 1 CTGTGCG 60 8.292495E-5 39.579147 9 CATATAG 185 0.0 38.54567 3 CGATGTA 50 0.0016047604 38.051003 2 GTATCAA 7515 0.0 36.962383 1 GTCTGGA 55 0.0025620079 34.59182 1 TAAGGTG 270 0.0 33.453835 5 GTATGGT 100 1.2846631E-6 33.29463 1 GGTACTC 190 0.0 32.527134 3 GTAAGGT 250 0.0 32.32697 4 GGGACTC 235 0.0 30.31594 7 GTGGTAC 225 0.0 29.595224 1 GGTATAT 180 2.0736479E-10 29.066738 1 GTACATG 6415 0.0 28.471523 1 ATCAACG 9725 0.0 28.401587 3 TCAACGC 9765 0.0 28.33393 4 AGGTAAG 170 3.5852281E-9 27.978678 2 CAACGCA 9890 0.0 27.975815 5 GGTAAGG 170 3.603418E-9 27.964504 3 >>END_MODULE