FastQCFastQC Report
Thu 26 May 2016
SRR938605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688941
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116511.6911462665162909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81871.1883455912770469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68280.9910863194380941No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53550.7772799122130923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.29915478974251786No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18230.2646090158663804No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.24602977613467628No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15510.2251281314365091No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.16474560230847055No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.15327872778655938No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8770.12729682222425434No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8420.12221656136011648No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT7770.11278179118386046No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7190.10436307318043199No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG7070.10262126945558474No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7010.1017503675931611No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC6960.1010246160411414No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6930.10058916510992959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG1201.8189894E-1243.5629433
GGTATCA47050.041.649531
AGGTAAG1400.040.734182
GTATCAA76050.036.300051
TAAGGTG2200.034.5623365
CATGGGT4250.033.5457954
GGTCCTA2550.033.536058
AGGGGTC2650.032.279925
GTAAGGT2500.030.4148544
GTATGGT1354.0842679E-728.254271
ATCAACG97300.028.1818033
GTACATG60500.028.055791
TCAACGC97900.027.960544
TATCAAC98200.027.8267272
CAACGCA98750.027.7679925
GGGGTCC3100.027.5941246
AACGCAG100500.027.284476
CATATAG1405.77571E-727.156123
ACATGGG62100.026.5548383
TACATGG64800.026.2551082
Produced by FastQC (version 0.11.5)