##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938605_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688941 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.379761111619136 31.0 31.0 34.0 30.0 34.0 2 31.49273740421894 33.0 31.0 34.0 30.0 34.0 3 31.488028728149434 33.0 31.0 34.0 28.0 34.0 4 35.20126106589679 37.0 35.0 37.0 33.0 37.0 5 35.240380526053755 37.0 35.0 37.0 33.0 37.0 6 35.30538173805885 37.0 35.0 37.0 33.0 37.0 7 35.25481717592653 37.0 35.0 37.0 32.0 37.0 8 35.23631196285313 37.0 35.0 37.0 32.0 37.0 9 36.741429237046425 39.0 37.0 39.0 32.0 39.0 10-11 36.61950442781022 39.0 37.0 39.0 32.0 39.0 12-13 36.49699829158085 39.0 37.0 39.0 32.0 39.0 14-15 37.42121966902826 39.5 37.0 41.0 32.0 41.0 16-17 37.33496917733159 39.0 37.0 41.0 32.0 41.0 18-19 37.401061919670916 39.5 37.0 41.0 32.0 41.0 20-21 37.460436379893196 39.5 37.0 41.0 32.0 41.0 22-23 37.487194839616166 40.0 37.0 41.0 32.0 41.0 24-25 37.390045011111255 40.0 37.0 41.0 32.0 41.0 26-27 37.19925944311632 39.0 36.0 41.0 31.5 41.0 28-29 37.01339592214718 39.0 36.0 41.0 31.0 41.0 30-31 36.66115748663529 39.0 35.5 40.5 31.0 41.0 32-33 36.25109189321002 38.0 35.0 40.0 30.0 41.0 34-35 35.959758527943606 38.0 35.0 40.0 30.0 41.0 36-37 35.662373120484915 38.0 35.0 40.0 29.5 41.0 38-39 35.3277341601095 38.0 35.0 40.0 28.0 41.0 40-41 35.09684864161082 38.0 35.0 40.0 26.5 41.0 42-43 34.760742937348766 38.0 34.0 40.0 24.5 41.0 44-45 34.29774538022849 38.0 33.5 40.0 23.5 41.0 46-47 33.72322593661866 38.0 33.0 40.0 20.0 41.0 48-49 33.214397894739896 38.0 33.0 40.0 14.0 41.0 50-51 32.41607409052445 37.0 32.0 39.5 9.0 40.5 52-53 32.45973385239084 37.0 32.5 39.5 7.5 40.5 54-55 32.595815171400744 37.0 33.0 40.0 4.0 41.0 56-57 32.35488017116124 37.0 32.0 40.0 2.0 41.0 58-59 32.00776191284885 36.5 31.5 40.0 2.0 41.0 60-61 31.61832871029595 36.0 31.0 39.5 2.0 41.0 62-63 31.13125071667966 35.5 30.5 39.0 2.0 41.0 64-65 30.710785829265497 35.0 29.5 39.0 2.0 40.0 66-67 30.226174665174522 35.0 29.0 39.0 2.0 40.0 68-69 29.685408474746023 35.0 28.5 38.0 2.0 40.0 70-71 29.10670797644501 34.5 27.5 37.5 2.0 40.0 72-73 28.51793912686282 34.0 26.0 37.0 2.0 39.0 74-75 27.86705900795569 34.0 26.0 36.0 2.0 39.0 76-77 27.200030191264563 33.5 24.5 36.0 2.0 38.5 78-79 26.490460721600254 33.0 22.0 35.0 2.0 37.5 80-81 25.809136776589 32.5 19.5 35.0 2.0 37.0 82-83 25.11760658750169 32.0 15.5 35.0 2.0 36.5 84-85 24.387925090827807 31.0 7.5 35.0 2.0 36.0 86-87 23.702493392032117 31.0 2.0 34.5 2.0 35.5 88-89 22.962631052586506 30.5 2.0 34.0 2.0 35.0 90-91 22.13879054955359 30.0 2.0 34.0 2.0 35.0 92-93 21.298322352712354 29.0 2.0 34.0 2.0 35.0 94-95 20.437747209122406 29.0 2.0 34.0 2.0 35.0 96-97 19.41183715296375 26.5 2.0 34.0 2.0 35.0 98-99 18.252578958140102 25.0 2.0 34.0 2.0 35.0 100-101 15.412745068155328 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7775780852555378 1101 2 0.6651577361394061 1101 3 0.7972397259902948 1101 4 0.6406838307370251 1101 5 0.6024707297742538 1101 6 0.5798942061493975 1101 7 0.5910122282593164 1101 8 0.44731672099524644 1101 9 0.7183714038362652 1101 10-11 0.7570745684435991 1101 12-13 0.844589192868753 1101 14-15 0.997845880157648 1101 16-17 1.2026511323728855 1101 18-19 1.2560448594953115 1101 20-21 1.1612324906199305 1101 22-23 1.0601516214239624 1101 24-25 1.1190514429706795 1101 26-27 1.2246116911058635 1101 28-29 1.3051739527505575 1101 30-31 1.0743826079341616 1101 32-33 1.1321840698881758 1101 34-35 1.1340261260030289 1101 36-37 1.0807147057911095 1101 38-39 1.2795824309461992 1101 40-41 1.170322644774565 1101 42-43 0.9654963297188459 1101 44-45 0.8853125794454613 1101 46-47 1.454039409503828 1101 48-49 1.131139837285616 1101 50-51 1.2129362624686308 1101 52-53 1.0988823133764 1101 54-55 1.4460497396898333 1101 56-57 1.4840631003929374 1101 58-59 1.5428143596116541 1101 60-61 1.594668423292859 1101 62-63 1.5128144864465831 1101 64-65 1.422791319930944 1101 66-67 1.6123625909892283 1101 68-69 1.5321681631528747 1101 70-71 1.6885222384237615 1101 72-73 1.8341147362734205 1101 74-75 2.009294598409152 1101 76-77 1.932643795781079 1101 78-79 2.302368294906767 1101 80-81 2.480057222469352 1101 82-83 2.726324702653802 1101 84-85 2.950345401226887 1101 86-87 3.0575599096473347 1101 88-89 3.2598012957178497 1101 90-91 3.833777694973538 1101 92-93 3.9871411498173828 1101 94-95 4.2676735819614535 1101 96-97 4.570011639486072 1101 98-99 4.924954399412105 1101 100-101 5.420871431539018 1102 1 0.24412220833064424 1102 2 -0.016295365099317394 1102 3 0.4038231220718558 1102 4 0.11951184154448669 1102 5 0.1375506783945255 1102 6 0.04561891123274364 1102 7 0.2351485912245721 1102 8 0.3025233472077673 1102 9 0.48264477939546424 1102 10-11 0.4743790523579037 1102 12-13 0.4635376802674358 1102 14-15 0.6941703106252319 1102 16-17 0.8835837924546794 1102 18-19 0.7287752193010206 1102 20-21 0.5152579737812673 1102 22-23 0.3926101706850105 1102 24-25 0.33396042113429303 1102 26-27 0.4777121916152325 1102 28-29 0.3943133423948879 1102 30-31 0.2764577286322165 1102 32-33 0.23886343384263853 1102 34-35 0.32949422775850934 1102 36-37 0.23549242943315818 1102 38-39 0.2966351837477319 1102 40-41 0.4010146469596805 1102 42-43 0.4559992013728902 1102 44-45 0.5393853083288391 1102 46-47 0.5341578190947089 1102 48-49 0.3432251453424868 1102 50-51 0.5735503092123722 1102 52-53 0.5280949338899035 1102 54-55 0.46698180920010657 1102 56-57 0.5219925566242054 1102 58-59 0.4034763902918428 1102 60-61 0.3968195800748333 1102 62-63 0.5602336888797375 1102 64-65 0.5944852267092315 1102 66-67 0.6976891032214425 1102 68-69 0.8533689427072879 1102 70-71 0.9404674333861855 1102 72-73 1.1770411658256172 1102 74-75 0.894088858053923 1102 76-77 0.8785806636849927 1102 78-79 1.2806795556293338 1102 80-81 1.9397433337035004 1102 82-83 2.038729657548899 1102 84-85 2.04660855989869 1102 86-87 2.356820957025935 1102 88-89 2.599092019315613 1102 90-91 2.9874923898695265 1102 92-93 2.7787284584352996 1102 94-95 3.387725699898585 1102 96-97 3.5364893232645507 1102 98-99 4.093459219952184 1102 100-101 4.288406238348307 1103 1 0.13951565473655236 1103 2 0.3032933045672337 1103 3 0.005126918079700005 1103 4 0.07640292265675441 1103 5 0.07088978073674213 1103 6 0.15189847740327167 1103 7 0.15206044076882108 1103 8 0.14495729205604135 1103 9 0.09919318138000222 1103 10-11 0.19562775433089996 1103 12-13 0.33729734335732076 1103 14-15 0.4398219201787086 1103 16-17 0.32060063796061655 1103 18-19 0.5716129947711934 1103 20-21 0.44977251168365484 1103 22-23 0.36889233290954593 1103 24-25 0.3357992927161533 1103 26-27 0.24824986324325238 1103 28-29 0.47937016713240155 1103 30-31 0.3517288720497689 1103 32-33 0.27207129123274143 1103 34-35 0.38374454505399314 1103 36-37 0.4008466465786569 1103 38-39 0.3231726246153883 1103 40-41 -0.06269107589712775 1103 42-43 0.2818616682010884 1103 44-45 0.10654378300288414 1103 46-47 0.14677608377683526 1103 48-49 0.3262498367005122 1103 50-51 0.39352838697861614 1103 52-53 0.6427467742037365 1103 54-55 0.6367348956198597 1103 56-57 0.4937099905690516 1103 58-59 0.8664898320825429 1103 60-61 0.6843830391725589 1103 62-63 0.6885941365548298 1103 64-65 0.7505369170288354 1103 66-67 0.657320463553738 1103 68-69 0.7500459935899464 1103 70-71 0.7886992321631574 1103 72-73 0.7352432542068357 1103 74-75 0.6832604283781407 1103 76-77 0.8662638297170666 1103 78-79 0.9396518066965989 1103 80-81 1.2301047621100984 1103 82-83 1.5382176580104208 1103 84-85 1.7492161519143856 1103 86-87 1.852378820185045 1103 88-89 2.1099161222347327 1103 90-91 2.490117288911872 1103 92-93 2.5655852139447006 1103 94-95 2.5404962664152606 1103 96-97 2.6147098724741973 1103 98-99 2.616261227567275 1103 100-101 2.9184469098609416 1104 1 0.7245507016712658 1104 2 0.5691811015415738 1104 3 0.43426694731483195 1104 4 0.4889506939182624 1104 5 0.4105940832322972 1104 6 0.3994658855151414 1104 7 0.3750377571246446 1104 8 0.3859600042308884 1104 9 0.47904955939377913 1104 10-11 0.556114757025469 1104 12-13 0.5661481032946227 1104 14-15 0.9137975898569479 1104 16-17 0.9586023462785462 1104 18-19 0.8467908711735106 1104 20-21 0.9241642536395887 1104 22-23 0.748106990981789 1104 24-25 0.8013412433686611 1104 26-27 1.019484959490235 1104 28-29 0.8159473232233481 1104 30-31 0.8894549600017285 1104 32-33 0.9980414661151826 1104 34-35 1.0953283054668148 1104 36-37 1.410794956752838 1104 38-39 1.5735204522337796 1104 40-41 1.5610485993463428 1104 42-43 1.5163591233357323 1104 44-45 1.7491101784520708 1104 46-47 1.6889655925914013 1104 48-49 1.8711614268451058 1104 50-51 1.9273072918939889 1104 52-53 1.935339146652268 1104 54-55 1.8608239394901673 1104 56-57 1.8802191221447515 1104 58-59 2.064301379959474 1104 60-61 2.182750670950796 1104 62-63 2.2849071637998613 1104 64-65 2.1565794312501367 1104 66-67 2.26238862015542 1104 68-69 2.206497095190837 1104 70-71 2.6374712685837878 1104 72-73 2.7517397495133658 1104 74-75 2.5853887811722096 1104 76-77 2.911772010270422 1104 78-79 3.1007763465955094 1104 80-81 3.144117592106049 1104 82-83 3.2010989475262797 1104 84-85 3.019288887512559 1104 86-87 2.989885204362853 1104 88-89 3.5601778735247755 1104 90-91 3.771575875410875 1104 92-93 3.7978933363800245 1104 94-95 4.132072894160455 1104 96-97 4.380052812894409 1104 98-99 4.942428871673581 1104 100-101 5.266108319487399 1105 1 0.5946029259157797 1105 2 0.4820395795778758 1105 3 0.30583998747093233 1105 4 0.5212198660369154 1105 5 0.5042656853770211 1105 6 0.5626051610984604 1105 7 0.4067432845321264 1105 8 0.4721710000168713 1105 9 0.6402744925762605 1105 10-11 0.6515664516489039 1105 12-13 0.7211042824803542 1105 14-15 0.7884809240101305 1105 16-17 0.9046789691653885 1105 18-19 0.7883009470913294 1105 20-21 0.7718510557449534 1105 22-23 0.6371181736928548 1105 24-25 0.7064091266880652 1105 26-27 0.9148899228430807 1105 28-29 1.102002138183913 1105 30-31 1.1945343211770663 1105 32-33 1.1579269745022103 1105 34-35 1.1892468864758783 1105 36-37 0.9141501799971294 1105 38-39 1.160523625041101 1105 40-41 1.0593023255502914 1105 42-43 1.239662860197683 1105 44-45 1.1437936765746244 1105 46-47 1.053970634649552 1105 48-49 1.0802941108597324 1105 50-51 1.098855121759371 1105 52-53 1.1015856418069703 1105 54-55 1.262958820694358 1105 56-57 1.4035386410586241 1105 58-59 1.2630947703541437 1105 60-61 1.4910294844684273 1105 62-63 1.5438516929618125 1105 64-65 1.5687574363987835 1105 66-67 1.4628760191092915 1105 68-69 1.4373065299884153 1105 70-71 1.4913120461265414 1105 72-73 1.664734527088914 1105 74-75 1.7172908668626015 1105 76-77 1.8946269629652583 1105 78-79 1.9504010616987273 1105 80-81 2.2420247280530567 1105 82-83 2.535876236128768 1105 84-85 2.5510733251796047 1105 86-87 2.7276448908534157 1105 88-89 3.1804514564194903 1105 90-91 3.314084934718597 1105 92-93 3.3239132258646684 1105 94-95 3.734648033124497 1105 96-97 3.677555764343076 1105 98-99 4.3405588010027785 1105 100-101 4.478632095046125 1106 1 0.20608684223100227 1106 2 0.30071980780908447 1106 3 0.22784652017580243 1106 4 0.15823301413912105 1106 5 0.34704663303269 1106 6 0.27033483942476977 1106 7 0.2465204283343283 1106 8 0.288986513123632 1106 9 0.4221503711837187 1106 10-11 0.25125325393122466 1106 12-13 0.3466076728714853 1106 14-15 0.5715465404824656 1106 16-17 0.448189677839828 1106 18-19 0.6188041307513004 1106 20-21 0.3045786667595891 1106 22-23 0.4020669043634868 1106 24-25 0.3763235399850018 1106 26-27 0.40717161473094876 1106 28-29 0.2546710375471761 1106 30-31 0.5878755102947295 1106 32-33 0.7988132310142433 1106 34-35 0.6621495987887869 1106 36-37 0.6617334926573477 1106 38-39 0.8238298765915815 1106 40-41 0.9379428613974738 1106 42-43 0.6367954989142959 1106 44-45 0.6624046498649534 1106 46-47 1.3644363999683264 1106 48-49 1.5088126753185094 1106 50-51 1.1176335472948011 1106 52-53 1.372963711764804 1106 54-55 1.369259986658136 1106 56-57 1.2132787799819624 1106 58-59 0.5167218686754893 1106 60-61 1.6840088940442186 1106 62-63 2.256375467045995 1106 64-65 2.0029838483726508 1106 66-67 1.9674923260637271 1106 68-69 1.279982044749019 1106 70-71 1.6475832360711387 1106 72-73 2.0866077181912033 1106 74-75 2.2857695322061886 1106 76-77 2.3465640339980887 1106 78-79 2.671145500630338 1106 80-81 3.139904699937617 1106 82-83 3.0605320287360627 1106 84-85 3.396334013180926 1106 86-87 3.7606433196965448 1106 88-89 3.5368001705516328 1106 90-91 3.6593507909990883 1106 92-93 3.8773635690384083 1106 94-95 3.74138333453854 1106 96-97 4.542462115124518 1106 98-99 4.801159975235809 1106 100-101 4.57272681926675 1107 1 0.2781086730422153 1107 2 0.15169475199166982 1107 3 0.29061010241345997 1107 4 0.18880607293346685 1107 5 0.21840361641149997 1107 6 0.25323734500651085 1107 7 0.4609386868309784 1107 8 0.1122859425456113 1107 9 0.2434354121164901 1107 10-11 0.26579058957995016 1107 12-13 0.34590313305506015 1107 14-15 0.4640843722859884 1107 16-17 0.620598509395272 1107 18-19 0.5375538206545443 1107 20-21 0.4165062281587453 1107 22-23 0.45568139208365466 1107 24-25 0.5779608508259955 1107 26-27 0.6370450952568731 1107 28-29 0.688841218643681 1107 30-31 0.7251665050851059 1107 32-33 0.830944215881523 1107 34-35 0.917034242797719 1107 36-37 1.0796099501120722 1107 38-39 0.9612994871100682 1107 40-41 0.8986126703778794 1107 42-43 1.008341021117225 1107 44-45 1.0174431018619785 1107 46-47 1.2202637380978203 1107 48-49 1.2074780189057108 1107 50-51 1.163021788426036 1107 52-53 1.2427925383586995 1107 54-55 1.4360622689702183 1107 56-57 1.4148317445068983 1107 58-59 1.4929510921932305 1107 60-61 1.4179260361925685 1107 62-63 1.5232769803181334 1107 64-65 1.5779528386976267 1107 66-67 1.8283357892242407 1107 68-69 1.7750651506780706 1107 70-71 1.6345591725633248 1107 72-73 1.675970159272822 1107 74-75 1.6784115565177764 1107 76-77 1.8059488020457195 1107 78-79 1.970257383537806 1107 80-81 2.0071684062139745 1107 82-83 2.263548649921873 1107 84-85 2.230757233225578 1107 86-87 2.4364954655660576 1107 88-89 2.482233065614892 1107 90-91 2.686038957707101 1107 92-93 2.9386833408071986 1107 94-95 3.1467169986417396 1107 96-97 3.065573005722385 1107 98-99 3.3703289159453043 1107 100-101 3.5538332444714147 1108 1 0.33317283243748363 1108 2 0.46680463953774876 1108 3 0.3529777007523158 1108 4 0.4479615019848424 1108 5 0.35878717294176 1108 6 0.3589496309474356 1108 7 0.2731303717733482 1108 8 0.3970267385887212 1108 9 0.3799759375236107 1108 10-11 0.4632848735610935 1108 12-13 0.48406934088484377 1108 14-15 0.3650493295908248 1108 16-17 0.5889583619453163 1108 18-19 0.6400091423847485 1108 20-21 0.6026160690434423 1108 22-23 0.5051928474520508 1108 24-25 0.6436320747539455 1108 26-27 0.5781668347930591 1108 28-29 0.7197151862606859 1108 30-31 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0.1362622886859377 2316 76-77 0.165485882820402 2316 78-79 0.2665776480630768 2316 80-81 0.40859495321444683 2316 82-83 0.5028401000872016 2316 84-85 0.5298266711093795 2316 86-87 0.40880533802708996 2316 88-89 0.26672527156670967 2316 90-91 0.4323970115568905 2316 92-93 0.6820891036791856 2316 94-95 0.9344354681740974 2316 96-97 0.89435430000675 2316 98-99 1.2474002432953313 2316 100-101 1.5430727343155315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3357.0 3 650.0 4 555.0 5 571.0 6 589.0 7 696.0 8 1002.0 9 1301.0 10 1768.0 11 2643.0 12 4032.0 13 5402.0 14 6785.0 15 9206.0 16 11189.0 17 13804.0 18 11571.0 19 8188.0 20 7981.0 21 8376.0 22 9440.0 23 10525.0 24 11661.0 25 13239.0 26 14741.0 27 17034.0 28 18963.0 29 21947.0 30 25588.0 31 29772.0 32 35405.0 33 42155.0 34 52278.0 35 68102.0 36 73548.0 37 83189.0 38 56150.0 39 5538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79882838658584 22.116969178181904 15.850991969906072 19.233210465326184 2 14.584334629028112 23.761164295743665 41.31417428135405 20.340326793874176 3 17.052380281628782 32.09964030396276 30.754955077929303 20.09302433647916 4 10.820191106851384 19.59716534518312 40.33777700328193 29.244866544683568 5 10.502878654962267 41.38988781669562 34.20327196463118 13.903961563710936 6 25.369050316328845 41.26464727445957 18.82679470868118 14.539507700530411 7 24.402252189467326 37.09724254884196 23.25321741352475 15.247287848165957 8 25.184359196616413 34.88574013012224 21.43009595825402 18.499804715007325 9 27.88010437070476 17.25035175018404 22.169191475521234 32.70035240358995 10-11 22.501628011821868 30.048560852069084 27.81203196928811 19.637779166820938 12-13 25.157939519073985 28.72539069249455 28.289795604264768 17.826874184166698 14-15 22.55456738464934 28.99971470406536 28.675871150773457 19.769846760511847 16-17 18.10331498490483 32.35571028598432 29.700571939607705 19.840402789503138 18-19 17.90479840931414 29.514003073641813 34.81236919418171 17.768829322862334 20-21 19.266065703266577 28.752294270712326 34.93404697737094 17.047593048650157 22-23 19.19595830238827 28.739089369345038 34.74246809845213 17.32248422981456 24-25 18.392073283640073 29.527018440725723 34.86070516986599 17.220203105768217 26-27 17.725809332537544 29.686635503025606 35.056772718889725 17.53078244554713 28-29 17.681784223598605 28.93264728709467 35.750249934476145 17.63531855483058 30-31 17.930355020521187 29.803892527500132 34.85937197110117 17.406380480877505 32-33 17.364167859116012 30.215178119625357 34.70154644526676 17.719107575991874 34-35 17.927174732146874 29.80585639674932 34.82729481049546 17.439674060608347 36-37 18.16215737463084 29.56248203204177 34.08605163737103 18.18930895595636 38-39 17.52898656451956 30.117572249551944 34.540849769276164 17.812591416652328 40-41 17.58065312261365 29.826048831760097 35.02222660979597 17.571071435830284 42-43 17.446367726415517 29.801425935416248 34.109429146747075 18.64277719142116 44-45 17.396741572136644 29.992212114987794 33.98580761383703 18.625238699038526 46-47 18.10093845426877 30.112194969699303 33.08876954266232 18.698097033369603 48-49 17.854630944872003 30.392873448252583 32.50970261758368 19.242792989291736 50-51 18.15540583205533 30.279017476424354 32.27096260049543 19.29461409102489 52-53 18.00978068220912 30.4918356415724 32.386062600430535 19.11232107578795 54-55 17.986809339283912 30.445284596452442 32.26159517431138 19.306310889952265 56-57 18.11869424571911 30.6694904736433 31.37183746078012 19.839977819857467 58-59 17.861448428380957 29.891862506650174 32.27336960900229 19.97331945596658 60-61 18.308140741740736 30.702445807984695 31.55083590630185 19.43857754397272 62-63 18.44179068947148 30.320110201634737 32.2564045990893 18.98169450980449 64-65 18.401192076436892 29.749289428378155 32.29660522411676 19.552913271068196 66-67 18.09274628133063 30.226331699138875 31.652979834451628 20.027942185078874 68-69 18.37955967036971 29.967135679996748 32.066082831441165 19.587221818192376 70-71 18.57350035639264 29.567015607239345 31.92994961145218 19.929534424915836 72-73 18.14677251588355 29.657548216824914 31.645886608167483 20.54979265912405 74-75 18.011201066104583 30.047629209291255 31.53058010083282 20.410589623771344 76-77 17.895163169274923 29.415035191665197 32.47504429352106 20.21475734553882 78-79 17.741039953955948 29.09898382289008 32.30980156204298 20.850174661110994 80-81 17.851191322953472 29.97998252281179 31.485720672726586 20.68310548150815 82-83 18.40626878064784 29.0611926128726 31.486382125033025 21.046156481446534 84-85 18.05435801856255 28.705687502358785 31.608952641403715 21.631001837674948 86-87 17.982339318205078 29.116174830153653 31.72819068368916 21.173295167952112 88-89 18.391424531287655 28.35785900737803 31.966298007264786 21.284418454069527 90-91 18.242000762264286 28.4922775367974 31.88094157788708 21.384780123051236 92-93 18.560978336137552 28.49633370739051 31.63202645595586 21.310661500516083 94-95 18.176862155825113 27.61522100510319 32.095065949461734 22.112850889609962 96-97 18.62945286271663 27.408111786589238 31.80437346883223 22.1580618818619 98-99 18.58392562655332 27.365616217220634 31.769200171881167 22.281257984344876 100-101 18.863015490133396 26.91047400389433 32.357304774913544 21.869205731058734 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3642.0 1 2306.0 2 1314.0 3 1594.5 4 1285.5 5 1214.0 6 2099.5 7 4183.5 8 6166.5 9 6270.5 10 4634.0 11 3186.5 12 2855.5 13 2763.5 14 2593.5 15 2401.5 16 2271.5 17 2281.5 18 2356.5 19 2448.0 20 2583.0 21 2769.0 22 2991.0 23 3263.0 24 3551.5 25 4079.0 26 4901.0 27 5619.0 28 6291.0 29 7755.5 30 10115.0 31 12232.0 32 15500.5 33 19966.5 34 23320.0 35 26489.0 36 32272.0 37 36183.5 38 35913.0 39 35099.0 40 32521.5 41 29161.0 42 25935.0 43 24560.0 44 24445.5 45 23662.0 46 21323.5 47 18663.5 48 18711.0 49 17337.0 50 13846.0 51 10937.0 52 8982.5 53 8169.5 54 7980.5 55 7496.0 56 6737.5 57 6302.5 58 5985.5 59 5598.0 60 4952.0 61 4527.0 62 3984.0 63 2669.0 64 1608.5 65 1196.5 66 981.0 67 759.5 68 565.0 69 437.0 70 369.0 71 319.0 72 265.5 73 250.5 74 210.0 75 161.0 76 126.0 77 89.0 78 57.5 79 35.5 80 22.0 81 13.5 82 13.0 83 10.0 84 3.5 85 5.0 86 5.0 87 3.0 88 2.5 89 2.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2939293785679761 2 0.0362875776009847 3 0.044125694362797396 4 0.04659324963966435 5 0.06168888192167399 6 0.06052767943844248 7 0.02743340866634443 8 0.029610663322403512 9 0.03512637511775319 10-11 0.03157019251285669 12-13 0.03309427077209805 14-15 0.02648993164871883 16-17 0.020974219853369155 18-19 0.02634478133831489 20-21 0.039045433498659535 22-23 0.032005643444068504 24-25 0.05566514403991053 26-27 0.04615779870845254 28-29 0.03781165586022606 30-31 0.038101956481033934 32-33 0.03374744716891577 34-35 0.03251366953048229 36-37 0.031207316736846843 38-39 0.018942115507714012 40-41 0.018434089421300228 42-43 0.018869540352512044 44-45 0.00733009067539891 46-47 0.013861854643576154 48-49 0.011684599987517074 50-51 0.00602373788176346 52-53 0.004644809932926042 54-55 0.006459188812975277 56-57 0.005297986329743766 58-59 0.007184940364994971 60-61 0.006967214899389063 62-63 0.0031933068288866532 64-65 0.00856386831383239 66-67 0.002685280742472868 68-69 0.006967214899389063 70-71 0.013644129177970248 72-73 0.01444245588519191 74-75 0.011321724211507227 76-77 0.010233096883477686 78-79 0.0051528360193398275 80-81 0.0062414633473693686 82-83 0.0097250707970639 84-85 0.003919058380906348 86-87 0.0013789279488374186 88-89 0.020175893146147494 90-91 0.029538088167201545 92-93 0.014224730419586001 94-95 0.022643448423014453 96-97 0.018651814886906135 98-99 0.013498978867566307 100-101 0.11045938621739743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 688941.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.61068095303798 #Duplication Level Percentage of deduplicated Percentage of total 1 76.471664655632 26.4673638734379 2 11.101837097131567 7.684842835228439 3 4.054024149005292 4.209376091914003 4 2.035626644401196 2.8181769731549235 5 1.2242278476901072 2.1185679725113333 6 0.752852474575535 1.5634042081343384 7 0.5710248462991917 1.3834491140063212 8 0.3881702058197956 1.0747868119283224 9 0.3155637168222392 0.9829687610960411 >10 2.4063718149289293 17.23300652355476 >50 0.3941554685876254 9.527576870439137 >100 0.2701939938928932 16.69718390574538 >500 0.010085001327261957 2.226640236196066 >1k 0.002521250331815489 1.3556181346560112 >5k 0.0012606251659077446 2.962551378923179 >10k+ 4.202083886359149E-4 1.694486309073832 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11651 1.6911462665162909 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8187 1.1883455912770469 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6828 0.9910863194380941 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5355 0.7772799122130923 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2061 0.29915478974251786 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1823 0.2646090158663804 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1695 0.24602977613467628 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1551 0.2251281314365091 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 0.16474560230847055 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1056 0.15327872778655938 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 877 0.12729682222425434 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 842 0.12221656136011648 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 777 0.11278179118386046 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 719 0.10436307318043199 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 707 0.10262126945558474 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 701 0.1017503675931611 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 696 0.1010246160411414 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 693 0.10058916510992959 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.451503104039388E-4 0.0 18-19 0.0 0.0 0.0 2.903006208078776E-4 0.0 20-21 0.0 0.0 0.0 8.709018624236328E-4 0.0 22-23 0.0 0.0 0.0 0.0027578558976748373 0.0 24-25 0.0 0.0 0.0 0.007620391296206787 0.0 26-27 0.0 0.0 0.0 0.01596653414443327 0.0 28-29 0.0 0.0 0.0 0.0362875776009847 0.0 30-31 0.0 0.0 0.0 0.08999319245044206 0.0 32-33 0.0 0.0 0.0 0.1622054718764016 0.0 34-35 0.0 0.0 0.0 0.2636655388487548 0.0 36-37 0.0 0.0 0.0 0.4146218616688512 0.0 38-39 0.0 0.0 0.0 0.6231302825641092 0.0 40-41 0.0 0.0 0.0 0.865676451249091 0.0 42-43 0.0 0.0 0.0 1.0926915367208512 0.0 44-45 0.0 0.0 0.0 1.3559216246383943 0.0 46-47 0.0 0.0 0.0 1.655439290156922 0.0 48-49 0.0 0.0 0.0 1.9817371879449763 0.0 50-51 0.0 0.0 0.0 2.321679214911001 0.0 52-53 0.0 0.0 0.0 2.6402841462476467 0.0 54-55 0.0 0.0 0.0 2.9792826961960457 0.0 56-57 0.0 0.0 0.0 3.3666888746641583 0.0 58-59 0.0 0.0 0.0 3.8030832828936005 0.0 60-61 0.0 0.0 0.0 4.267564276186205 0.0 62-63 0.0 0.0 0.0 4.7104178732286215 0.0 64-65 0.0 0.0 0.0 5.15515842430629 0.0 66-67 0.0 0.0 0.0 5.655346393958263 0.0 68-69 0.0 0.0 0.0 6.186015928795063 0.0 70-71 0.0 0.0 0.0 6.761391759236277 0.0 72-73 0.0 0.0 0.0 7.344750856749707 0.0 74-75 0.0 0.0 0.0 7.914175524464359 0.0 76-77 0.0 0.0 0.0 8.497534621977788 0.0 78-79 0.0 0.0 0.0 9.177563826220243 0.0 80-81 0.0 0.0 0.0 9.893445157132469 0.0 82-83 0.0 0.0 0.0 10.603447900473334 0.0 84-85 0.0 0.0 0.0 11.313087768038192 0.0 86-87 0.0 0.0 0.0 12.036081464160212 0.0 88-89 0.0 0.0 0.0 12.753922324262891 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAAGG 120 1.8189894E-12 43.562943 3 GGTATCA 4705 0.0 41.64953 1 AGGTAAG 140 0.0 40.73418 2 GTATCAA 7605 0.0 36.30005 1 TAAGGTG 220 0.0 34.562336 5 CATGGGT 425 0.0 33.545795 4 GGTCCTA 255 0.0 33.53605 8 AGGGGTC 265 0.0 32.27992 5 GTAAGGT 250 0.0 30.414854 4 GTATGGT 135 4.0842679E-7 28.25427 1 ATCAACG 9730 0.0 28.181803 3 GTACATG 6050 0.0 28.05579 1 TCAACGC 9790 0.0 27.96054 4 TATCAAC 9820 0.0 27.826727 2 CAACGCA 9875 0.0 27.767992 5 GGGGTCC 310 0.0 27.594124 6 AACGCAG 10050 0.0 27.28447 6 CATATAG 140 5.77571E-7 27.15612 3 ACATGGG 6210 0.0 26.554838 3 TACATGG 6480 0.0 26.255108 2 >>END_MODULE