FastQCFastQC Report
Thu 26 May 2016
SRR938605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688941
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107371.558478882807091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80571.169476050924535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66560.9661204660486167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57640.8366463891683033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.31076681457483296No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.24312676992659749No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15810.22948264074862723No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.20103317990945524No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12500.1814378880049235No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.15473023089059876No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.149359669405653No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA9380.1361509911588946No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8330.12091020856648102No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT8080.11728145080638255No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT7140.1036373216284123RNA PCR Primer, Index 39 (95% over 23bp)
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC7100.10305672038679656No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7050.10233096883477685No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7000.10160521728275716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45500.039.4294171
GTATCAA73450.035.633731
CGTCACT1106.893606E-834.6088941
AGATAGT902.4136612E-531.6626516
ATGCCGC300.00395367631.66265194-95
AGGCTTA4000.030.8710866
CAACGGA650.005846091529.2270629
GATAGTC650.005846091529.2270627
GTACATG58050.028.6916981
CATATAG1703.587047E-927.9762133
GTATTAT2053.092282E-1127.855941
GTGGTAC2253.6379788E-1227.4948431
CGACCGT350.00839733327.13941418-19
GTTCGAC350.00839733327.13941416-17
TCGACCG350.00839733327.13941418-19
ATCAACG96000.027.0990433
TCAACGC96350.027.00064
TATCAAC96850.026.8768352
ACATGGG59650.026.8693583
CAACGCA97850.026.4894855
Produced by FastQC (version 0.11.5)