##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938605_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688941 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.735666769723387 31.0 31.0 34.0 30.0 34.0 2 31.941156064162243 31.0 31.0 34.0 30.0 34.0 3 31.96815257039427 33.0 31.0 34.0 30.0 34.0 4 35.6963252295915 37.0 35.0 37.0 33.0 37.0 5 35.587404436664386 37.0 35.0 37.0 33.0 37.0 6 35.64870431575418 37.0 35.0 37.0 33.0 37.0 7 35.58752345991892 37.0 35.0 37.0 33.0 37.0 8 35.580458994311556 37.0 35.0 37.0 33.0 37.0 9 37.090549118139286 39.0 37.0 39.0 33.0 39.0 10-11 37.03104474838919 39.0 37.0 39.0 33.0 39.0 12-13 36.9312248799244 39.0 37.0 39.0 33.0 39.0 14-15 37.81381642259642 40.0 37.0 41.0 32.0 41.0 16-17 37.79500494236807 39.0 37.0 41.0 32.5 41.0 18-19 37.944167933103124 39.5 37.0 41.0 33.0 41.0 20-21 37.999194415777254 40.0 37.0 41.0 33.0 41.0 22-23 37.95230433375282 40.0 37.0 41.0 33.0 41.0 24-25 37.85171879740065 40.0 37.0 41.0 33.0 41.0 26-27 37.63384672998123 40.0 36.5 41.0 32.0 41.0 28-29 37.46678162571251 39.5 36.0 41.0 32.0 41.0 30-31 37.10575288740255 39.0 35.5 40.5 32.0 41.0 32-33 36.72413240030714 38.5 35.0 40.0 31.0 41.0 34-35 36.42984812923022 38.0 35.0 40.0 30.0 41.0 36-37 36.285429376390724 38.0 35.0 40.0 30.0 41.0 38-39 36.065055643371494 38.0 35.0 40.0 30.0 41.0 40-41 35.81827616588358 38.0 35.0 40.0 30.0 41.0 42-43 35.475262032597854 38.0 35.0 40.0 28.5 41.0 44-45 34.993373888330055 38.0 34.5 40.0 26.0 41.0 46-47 34.39213735283573 38.0 33.5 40.0 23.5 41.0 48-49 33.84623284141893 38.0 33.0 40.0 21.0 41.0 50-51 33.45258810260966 38.0 33.0 40.0 18.0 41.0 52-53 32.98137431216897 37.0 33.0 40.0 14.0 41.0 54-55 32.700648531586886 37.0 32.5 40.0 9.0 41.0 56-57 32.37176187801278 37.0 32.0 40.0 7.5 41.0 58-59 31.961049639954656 36.0 31.0 39.5 4.0 41.0 60-61 31.592951355776474 36.0 31.0 39.0 2.0 40.5 62-63 31.150390962361072 35.0 30.5 39.0 2.0 40.0 64-65 30.65412350259311 35.0 29.0 39.0 2.0 40.0 66-67 30.177544230928337 35.0 29.0 38.0 2.0 40.0 68-69 29.651294523043337 35.0 28.0 38.0 2.0 40.0 70-71 29.13292865426793 34.0 27.5 37.0 2.0 39.5 72-73 28.587320975235905 34.0 26.0 36.5 2.0 39.0 74-75 27.993468961783375 33.5 26.0 36.0 2.0 39.0 76-77 26.685717644907186 31.5 25.0 34.5 2.0 37.0 78-79 27.311636990685706 33.0 26.0 35.0 2.0 37.0 80-81 27.033358444337033 33.0 25.5 35.0 2.0 37.0 82-83 26.573266796431042 33.0 24.5 35.0 2.0 36.5 84-85 25.976172850795642 32.5 22.5 35.0 2.0 36.0 86-87 25.395570157676783 32.0 20.0 35.0 2.0 35.5 88-89 24.694274255705494 31.0 17.0 34.0 2.0 35.0 90-91 24.17912564356019 31.0 9.0 34.0 2.0 35.0 92-93 23.46840585768593 31.0 2.0 34.0 2.0 35.0 94-95 22.771664772455114 30.5 2.0 34.0 2.0 35.0 96-97 21.879729178550846 30.0 2.0 34.0 2.0 35.0 98-99 20.85462035210562 29.0 2.0 34.0 2.0 35.0 100-101 17.908677956457808 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23672773937305536 1101 2 0.2957731759356754 1101 3 0.34275700713115853 1101 4 0.12535584451643444 1101 5 0.22394564745860635 1101 6 0.1336595937360343 1101 7 0.1391145983666462 1101 8 0.23377453193773334 1101 9 0.31922394413022204 1101 10-11 0.3727165611024148 1101 12-13 0.3418278342469918 1101 14-15 0.407908540535054 1101 16-17 0.6241964887689448 1101 18-19 0.6273635616176918 1101 20-21 0.4305458562199007 1101 22-23 0.3814598048077329 1101 24-25 0.49530213402413636 1101 26-27 0.38207746189382163 1101 28-29 0.6644950988519227 1101 30-31 0.45148908237116814 1101 32-33 0.5157627146873622 1101 34-35 0.4332473669259045 1101 36-37 0.9091352580382406 1101 38-39 1.0024029338295861 1101 40-41 1.2056119887044687 1101 42-43 1.3063120635998544 1101 44-45 1.5021267167734678 1101 46-47 1.2858352967614337 1101 48-49 1.2851073155155603 1101 50-51 1.3851953964193413 1101 52-53 1.2475213279367452 1101 54-55 1.1888845295108936 1101 56-57 1.2508070280033579 1101 58-59 1.3152038917265436 1101 60-61 1.0388363086891772 1101 62-63 1.483782756561272 1101 64-65 1.577359108649862 1101 66-67 1.7177592216100805 1101 68-69 1.5578336602453575 1101 70-71 1.6590704093456239 1101 72-73 1.6497341413432345 1101 74-75 1.1965651571850664 1101 76-77 1.2111297747586853 1101 78-79 1.3417394560387699 1101 80-81 1.788872615278322 1101 82-83 1.7820189283529295 1101 84-85 1.7817459633717618 1101 86-87 1.9786851665519833 1101 88-89 2.2702258559595023 1101 90-91 2.248678719475418 1101 92-93 2.4406063163184903 1101 94-95 2.5718873075843547 1101 96-97 2.780930970937259 1101 98-99 2.9942316675084975 1101 100-101 3.9099031883845647 1102 1 0.3131403126408365 1102 2 0.38097050064666504 1102 3 0.2244876769869144 1102 4 0.17917167478617557 1102 5 0.16881579554441828 1102 6 0.050780262263003806 1102 7 0.11168255100281499 1102 8 0.1491825430404745 1102 9 0.4098840554529559 1102 10-11 0.45796925189393534 1102 12-13 0.32444657603436156 1102 14-15 0.6388995195549967 1102 16-17 0.443463146577038 1102 18-19 0.5838901151899094 1102 20-21 0.7180582213112174 1102 22-23 0.6569724947133864 1102 24-25 0.594536605684084 1102 26-27 0.3861634786365258 1102 28-29 0.4774795520565078 1102 30-31 0.3310124911500125 1102 32-33 0.2505702621914665 1102 34-35 0.4575826048523979 1102 36-37 0.589211589407924 1102 38-39 0.5325811387874353 1102 40-41 0.700750846834417 1102 42-43 0.7587895111497218 1102 44-45 0.4719079100232335 1102 46-47 0.7040153029624392 1102 48-49 0.9018153221890159 1102 50-51 0.9538530273416583 1102 52-53 1.1114757948721206 1102 54-55 0.9400213796976615 1102 56-57 1.0119503744769105 1102 58-59 1.3468954362187198 1102 60-61 1.3839810725343824 1102 62-63 1.3824665202735993 1102 64-65 1.2106953948408226 1102 66-67 1.1034651760474894 1102 68-69 1.0099383095196934 1102 70-71 1.0882055173463954 1102 72-73 1.4206551376374001 1102 74-75 1.359684703626133 1102 76-77 1.2053938488163567 1102 78-79 1.3798571569438174 1102 80-81 1.4773572087436477 1102 82-83 1.64817327422114 1102 84-85 1.7393835296265046 1102 86-87 1.8363315913796114 1102 88-89 1.7871678765999768 1102 90-91 2.0968280159570902 1102 92-93 2.3047194993513678 1102 94-95 2.778450771876919 1102 96-97 3.133210577026052 1102 98-99 3.3559050519262676 1102 100-101 4.106390026021689 1103 1 0.16125673124011897 1103 2 0.15404653618375974 1103 3 0.14077935807609876 1103 4 0.22810729264703156 1103 5 -0.01266280143262577 1103 6 -0.016053705661306594 1103 7 -0.0738776197598483 1103 8 0.07608920047672996 1103 9 -0.006897991391397795 1103 10-11 0.3229023313908783 1103 12-13 0.1899277113753115 1103 14-15 0.23480208099494604 1103 16-17 0.33142178278333745 1103 18-19 0.3294984571767543 1103 20-21 0.3278785166844713 1103 22-23 0.41046995634948047 1103 24-25 0.42819444576716137 1103 26-27 0.29931826114489724 1103 28-29 0.2600095509027085 1103 30-31 0.15841816785475515 1103 32-33 0.16042949837468967 1103 34-35 0.10434619086831987 1103 36-37 0.263839488334888 1103 38-39 0.5478618010696579 1103 40-41 0.51317152296226 1103 42-43 0.19723418635452106 1103 44-45 0.303308625380339 1103 46-47 0.6059782082353067 1103 48-49 0.37416734711113975 1103 50-51 0.38153850097729247 1103 52-53 0.5930942903146104 1103 54-55 0.46210947246333234 1103 56-57 0.2540324097832425 1103 58-59 0.2456204852552979 1103 60-61 0.06039736421547559 1103 62-63 0.24498382730025625 1103 64-65 -0.0687265504689698 1103 66-67 0.020030885058677228 1103 68-69 0.013212222061515178 1103 70-71 0.1796587306843449 1103 72-73 0.18685163007999606 1103 74-75 0.31776564282077757 1103 76-77 0.2371522427242816 1103 78-79 0.644135453932396 1103 80-81 0.843674850372814 1103 82-83 0.7088993039892308 1103 84-85 0.9180713685557791 1103 86-87 1.0787597672190046 1103 88-89 0.941719624609668 1103 90-91 1.0447482882543078 1103 92-93 1.082994854145415 1103 94-95 1.007365044404903 1103 96-97 1.4156107731723502 1103 98-99 1.828321978198332 1103 100-101 2.5000383472401033 1104 1 0.2715775251676682 1104 2 0.2823597043209922 1104 3 0.1984923254419435 1104 4 0.2062535915850532 1104 5 0.22271743139173594 1104 6 0.2770769190194855 1104 7 0.23333341914401018 1104 8 0.24773112295079613 1104 9 0.5008466767194193 1104 10-11 0.37846889414954177 1104 12-13 0.4601039146827546 1104 14-15 0.6951398260152928 1104 16-17 0.540808505454649 1104 18-19 0.6139895281335868 1104 20-21 0.7306948196731469 1104 22-23 0.5389779519818205 1104 24-25 0.5304392547701724 1104 26-27 0.6504843642427645 1104 28-29 0.7539148625386431 1104 30-31 0.7212582904309741 1104 32-33 0.6198613505787662 1104 34-35 0.4838701513480643 1104 36-37 0.5513784538339053 1104 38-39 0.5191979434412275 1104 40-41 0.5103008443902084 1104 42-43 0.690819188379848 1104 44-45 0.9525574938398549 1104 46-47 0.786479828495608 1104 48-49 0.695077951010326 1104 50-51 0.8187534144166477 1104 52-53 0.8353793416521995 1104 54-55 0.9660489504577257 1104 56-57 0.8101625413413345 1104 58-59 1.0680214644560841 1104 60-61 1.1651859378580696 1104 62-63 1.2478401965082675 1104 64-65 1.2808666233039467 1104 66-67 1.557255044548647 1104 68-69 1.3464024617543728 1104 70-71 1.3154854156669948 1104 72-73 1.5468378050328866 1104 74-75 1.6873952284215967 1104 76-77 1.6022523642438067 1104 78-79 1.9486607352765084 1104 80-81 1.932078101460327 1104 82-83 2.0899653736252013 1104 84-85 2.1525060826326694 1104 86-87 2.260966403241614 1104 88-89 2.287838925079896 1104 90-91 2.669316805892251 1104 92-93 3.070060289766488 1104 94-95 3.2504789381369577 1104 96-97 3.965527338572752 1104 98-99 4.24533537176308 1104 100-101 4.378730163164445 1105 1 0.24879398113369078 1105 2 0.22806739612416393 1105 3 0.24577084381471437 1105 4 0.2343333462868742 1105 5 0.1596445634034751 1105 6 0.24363126666734303 1105 7 0.22635652242831128 1105 8 0.07406706339711633 1105 9 0.10862989281464763 1105 10-11 0.3211728720464748 1105 12-13 0.3086218365349467 1105 14-15 0.308157764264422 1105 16-17 0.44357056950109097 1105 18-19 0.4522634635624527 1105 20-21 0.32433977679515635 1105 22-23 0.17837651233074325 1105 24-25 0.14682471396638874 1105 26-27 0.1548684952870829 1105 28-29 0.2170125308941877 1105 30-31 0.26266465572193454 1105 32-33 0.2517183446922715 1105 34-35 0.4668142623881195 1105 36-37 0.4317832942097226 1105 38-39 -0.002452162319016793 1105 40-41 -0.07218394745068224 1105 42-43 0.22503857119913562 1105 44-45 0.19924838698101155 1105 46-47 0.3983366586397352 1105 48-49 0.7017535540076878 1105 50-51 0.5756370535528603 1105 52-53 0.5752675555338485 1105 54-55 0.3916273524122502 1105 56-57 0.37879294618171144 1105 58-59 0.39842487009991245 1105 60-61 0.6603601143219038 1105 62-63 0.6140770584624526 1105 64-65 0.4814011634518458 1105 66-67 0.6622456573021829 1105 68-69 0.9978599444965965 1105 70-71 1.014721523362077 1105 72-73 0.8983245973000926 1105 74-75 0.9667652171077101 1105 76-77 1.0936337242057625 1105 78-79 1.3615684041239682 1105 80-81 1.4110705506708143 1105 82-83 1.2231074776801627 1105 84-85 1.0082161109993741 1105 86-87 1.3899826280108272 1105 88-89 1.2955670064742897 1105 90-91 1.6582806424475827 1105 92-93 1.6961014952692963 1105 94-95 1.7626864577722898 1105 96-97 2.3213685424358665 1105 98-99 2.6190307904588686 1105 100-101 3.297132855541509 1106 1 0.2214641208839616 1106 2 0.21251087582315975 1106 3 -0.005699014781168188 1106 4 0.1551056939260036 1106 5 0.249942669745991 1106 6 0.0535750357899758 1106 7 0.11099226211242552 1106 8 0.087955263182117 1106 9 0.04418558619098434 1106 10-11 0.27565790470999474 1106 12-13 0.15504228638197048 1106 14-15 0.005488864901153079 1106 16-17 0.0784874635720314 1106 18-19 0.11762462291083153 1106 20-21 0.44279880267789906 1106 22-23 0.2584393586054574 1106 24-25 0.1720293778215165 1106 26-27 0.3107169199955919 1106 28-29 0.5007913698919637 1106 30-31 0.4243987167489749 1106 32-33 0.38551165487171346 1106 34-35 0.368121837018073 1106 36-37 0.34506783898770266 1106 38-39 0.16526676598661538 1106 40-41 0.3137666024542227 1106 42-43 0.21956743087002195 1106 44-45 -0.2968867738558316 1106 46-47 -0.35593337361032695 1106 48-49 0.14281243104399266 1106 50-51 -0.2941254123365056 1106 52-53 -0.03475787239966621 1106 54-55 0.2672620170942892 1106 56-57 -0.1518229059641527 1106 58-59 0.08953646358374456 1106 60-61 -0.25946230360582234 1106 62-63 0.14392800529119398 1106 64-65 -0.3615989192406204 1106 66-67 -0.20851738743443704 1106 68-69 -0.0472705028696474 1106 70-71 0.08253864897193353 1106 72-73 0.6054878322293895 1106 74-75 0.6177978392858314 1106 76-77 0.9789454748052862 1106 78-79 0.9625607137245602 1106 80-81 0.8548903224396049 1106 82-83 1.0882391654334072 1106 84-85 1.2441449541317624 1106 86-87 1.4429151096117536 1106 88-89 1.9610108582893915 1106 90-91 1.5602270163829175 1106 92-93 1.5323187283793587 1106 94-95 1.9432785358339828 1106 96-97 2.7921439910425008 1106 98-99 2.9154222979425377 1106 100-101 3.431319988593689 1107 1 0.1947709926279444 1107 2 0.23019383290577267 1107 3 0.11111567140092049 1107 4 0.3003103577811288 1107 5 -0.0030565860218061403 1107 6 0.11616000229953016 1107 7 0.30934502817544285 1107 8 0.10590614385689179 1107 9 0.20500920315947724 1107 10-11 0.14855792951773594 1107 12-13 0.3422712618223116 1107 14-15 0.36993937345982886 1107 16-17 0.3715016039838446 1107 18-19 0.3234990957463566 1107 20-21 0.34014437449630464 1107 22-23 0.42918111003189807 1107 24-25 0.29303161051811344 1107 26-27 0.37814435735604945 1107 28-29 0.317615979170057 1107 30-31 0.3395612074460743 1107 32-33 0.32329880446238235 1107 34-35 0.34563610146857826 1107 36-37 0.3231626045545539 1107 38-39 0.5080650790602874 1107 40-41 0.6073562824343739 1107 42-43 0.48679144476235336 1107 44-45 0.05870240996951992 1107 46-47 0.31876769312248854 1107 48-49 0.45824780688125344 1107 50-51 0.10124050182871969 1107 52-53 -0.03430140998339226 1107 54-55 -0.07170598092108804 1107 56-57 0.017499842733435855 1107 58-59 0.0875915367665776 1107 60-61 0.1031474706437443 1107 62-63 0.20766901248543945 1107 64-65 0.3009413933369025 1107 66-67 0.3846307147734507 1107 68-69 0.4504174157609633 1107 70-71 0.39147439692529673 1107 72-73 0.5943590800587124 1107 74-75 0.5701272860732267 1107 76-77 0.5371394713321997 1107 78-79 0.7305339213653461 1107 80-81 0.9652946886018476 1107 82-83 1.2027913857261368 1107 84-85 1.377670133987877 1107 86-87 1.6191492946774915 1107 88-89 1.7031244777386583 1107 90-91 1.8552059298039048 1107 92-93 2.3623658630853903 1107 94-95 2.5022266876573482 1107 96-97 2.728983484964605 1107 98-99 2.6917606755163384 1107 100-101 2.589489177380589 1108 1 0.17403467607715228 1108 2 0.21576240180129602 1108 3 0.2893117425128615 1108 4 0.2177141186882281 1108 5 0.20219001936291647 1108 6 -0.0028769068507656925 1108 7 0.12711554751658127 1108 8 0.20931895707138892 1108 9 0.1491144712290975 1108 10-11 0.2524513549295264 1108 12-13 0.4293591251977986 1108 14-15 0.5074271248480002 1108 16-17 0.4767351441826264 1108 18-19 0.4410073781410162 1108 20-21 0.3836794405221511 1108 22-23 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2316 54-55 -0.5854235057203354 2316 56-57 -0.5233267987403494 2316 58-59 -0.45837917650692006 2316 60-61 -0.2555251793326363 2316 62-63 -0.08918030258305265 2316 64-65 -0.1636783515851583 2316 66-67 -0.38963003156570863 2316 68-69 -0.2830544784289266 2316 70-71 -0.3407835152145253 2316 72-73 -0.5657401169181426 2316 74-75 -0.33926336106895505 2316 76-77 -0.06515623013213556 2316 78-79 0.2568447385594901 2316 80-81 0.25290451382622336 2316 82-83 0.5689132562977015 2316 84-85 0.5547934216591095 2316 86-87 0.40875723043563994 2316 88-89 0.8360625977197635 2316 90-91 0.8884274697188772 2316 92-93 0.7641655796654554 2316 94-95 1.2600963144877682 2316 96-97 1.003664637539 2316 98-99 1.5598098016382096 2316 100-101 1.8843356582497428 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 21.0 5 42.0 6 138.0 7 286.0 8 558.0 9 965.0 10 1527.0 11 2606.0 12 3549.0 13 5149.0 14 6231.0 15 8444.0 16 10745.0 17 13505.0 18 11738.0 19 7929.0 20 7160.0 21 7379.0 22 8244.0 23 8897.0 24 9996.0 25 11155.0 26 12861.0 27 14843.0 28 17332.0 29 20632.0 30 23973.0 31 28671.0 32 34074.0 33 41638.0 34 53073.0 35 70352.0 36 81791.0 37 96956.0 38 62274.0 39 4204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.27746973611635 22.95540975992104 16.3853165732865 19.38180393067611 2 14.644838737314114 24.34920621091135 40.521651499508465 20.48430355226607 3 16.93311463988156 31.983191569657738 31.2420530089703 19.841640781490405 4 10.99092665409665 20.45066268374215 39.457370079585914 29.101040582575287 5 10.314805720147508 41.79494183664277 34.37958601289678 13.510666430312945 6 25.165725366903697 41.669025359210735 18.497665257257154 14.667584016628417 7 24.184218967952262 37.0574548473672 23.093559535577064 15.664766649103479 8 25.11245520298545 35.40970852366168 21.29964684929479 18.178189424058083 9 27.226134023087607 17.325431350434943 22.12656816766603 33.32186645881142 10-11 22.384717994719434 30.211585607475822 27.729007273482054 19.674689124322693 12-13 24.889722051670606 28.794337976691764 28.30960851509781 18.00633145653982 14-15 22.1698229601664 29.26585876003896 28.426527090128182 20.13779118966646 16-17 17.964165291367475 32.61121053907374 29.608268342281853 19.816355827276936 18-19 17.784541782242602 29.785279145819455 34.51623578797023 17.91394328396771 20-21 18.938559325109118 29.07034129192485 34.69970578032081 17.29139360264522 22-23 18.942550958645224 28.996459783929247 34.56232101152349 17.498668245902042 24-25 18.18100005298044 29.92298966445963 34.72113055904525 17.174879723514678 26-27 17.669115901834132 29.945002899741528 34.85228012591919 17.53360107250515 28-29 17.386249330494195 29.19444480732022 35.66002023395327 17.759285628232316 30-31 17.678146604716513 29.86554726747283 34.928462669517415 17.527843458293233 32-33 17.110175371368687 30.419718465231995 34.64934435319289 17.820761810206434 34-35 17.78010702267336 29.998649876746814 34.81982387424401 17.401419226335825 36-37 17.910409847368687 29.702285477889145 34.06595879027166 18.321345884470507 38-39 17.36368129989715 30.152448739174964 34.5379176589541 17.945952301973783 40-41 17.32910914659815 29.942298480163743 35.00435483168576 17.724237541552352 42-43 17.393690026107084 29.867606531343377 34.038135067321726 18.70056837522781 44-45 17.26988486259573 30.1827073257117 33.76275191750518 18.78465589418739 46-47 17.97193083297409 30.147646895742884 32.93090409773841 18.949518173544615 48-49 17.662688096658492 30.509869495355918 32.273881217694985 19.553561190290605 50-51 18.080648415466634 30.372339576248187 32.03177049994122 19.515241508343966 52-53 17.886364724990962 30.66489002686732 32.021609978212936 19.427135269928776 54-55 17.81139458966733 30.632086056716034 31.915795401928467 19.640723951688173 56-57 18.053723032886708 30.795815606851672 30.96585919548989 20.18460216477173 58-59 17.645342634565225 30.13755894916981 31.95846959318723 20.258628823077736 60-61 18.173544614125156 31.16275559155283 31.026023999152326 19.637675795169688 62-63 18.19618806254817 30.89234056327029 31.63064761713993 19.280823757041603 64-65 18.07106849497998 30.364574034641574 31.555967782437104 20.008389687941346 66-67 17.81050281664995 30.758238392658853 31.019364559126423 20.411894231564766 68-69 18.036997443171714 30.552610207427506 31.215585693663954 20.194806655736823 70-71 18.276673909667156 30.16687931187141 30.994381231484265 20.562065546977173 72-73 17.805370851785565 30.11832653304129 30.708870570919718 21.367432044253427 74-75 17.554696265717965 30.47655749911821 30.51843336366975 21.450312871494077 76-77 17.160468022660865 29.8571285494694 31.918553257826144 21.063850170043587 78-79 17.132671738218512 29.397437516420126 31.674337860571516 21.795552884789842 80-81 17.113221596624385 30.419876302905475 30.862584749637488 21.604317350832655 82-83 17.514825451222794 29.42475580008811 30.96264008064639 22.097778668042707 84-85 17.059193250816868 29.116521244337097 30.965375889263232 22.85890961558281 86-87 16.88147874510749 29.509729140100927 31.123015144378535 22.48577697041305 88-89 17.139070790583514 28.982256087427 30.960075652396686 22.918597469592804 90-91 17.014182719174613 28.77360339189215 31.183910865579872 23.02830302335337 92-93 17.109775241512498 28.931205134848557 30.789931481646793 23.16908814199215 94-95 16.607082464245853 28.046015551404256 31.342451675832912 24.004450308516983 96-97 16.902753646538674 27.93526586456605 30.93900638806516 24.222974100830115 98-99 16.61223530026519 27.99267281233081 30.96673009735231 24.42836179005169 100-101 16.548585860785124 27.685122689368374 31.448757865400946 24.31753358444556 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3747.0 1 2329.0 2 1202.0 3 1523.0 4 1378.0 5 1372.0 6 2199.5 7 4101.5 8 5746.5 9 5805.5 10 4461.5 11 3141.0 12 2793.0 13 2675.5 14 2504.5 15 2327.5 16 2218.0 17 2189.0 18 2213.5 19 2286.0 20 2335.0 21 2491.0 22 2750.0 23 3063.0 24 3298.0 25 3697.0 26 4442.0 27 5272.5 28 6004.0 29 7318.0 30 9772.0 31 12014.5 32 15579.5 33 20010.0 34 23288.0 35 27002.5 36 32916.5 37 36489.0 38 36154.5 39 35487.0 40 32768.0 41 29281.0 42 26024.0 43 25001.5 44 25345.5 45 24468.0 46 22188.0 47 19728.0 48 19608.5 49 17837.5 50 14238.5 51 11226.5 52 8908.5 53 8020.5 54 7769.0 55 7340.0 56 6717.5 57 6095.0 58 5836.5 59 5592.5 60 4822.5 61 4230.0 62 3708.5 63 2437.0 64 1348.5 65 1051.0 66 826.0 67 586.0 68 441.5 69 335.5 70 278.0 71 247.5 72 196.0 73 185.0 74 165.0 75 123.0 76 93.5 77 52.5 78 36.0 79 27.0 80 13.5 81 5.5 82 3.5 83 2.0 84 1.5 85 1.0 86 2.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.451503104039388E-4 2 0.04093238753391074 3 1.451503104039388E-4 4 0.0 5 0.10262126945558474 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001088627328029541 26-27 0.012555501849940707 28-29 0.0 30-31 0.0 32-33 4.354509312118164E-4 34-35 0.016692285696452962 36-37 0.003773908070502409 38-39 0.012555501849940707 40-41 0.007402665830600878 42-43 0.006894639744187093 44-45 0.00362875776009847 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.903006208078776E-4 68-69 2.177254656059082E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001088627328029541 84-85 0.0040642086913102865 86-87 7.983267072216633E-4 88-89 7.25751552019694E-5 90-91 5.806012416157552E-4 92-93 2.177254656059082E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.709018624236328E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 688941.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.87305341256068 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84751393516817 24.604663233881627 2 11.757316848891177 7.729978095239984 3 4.324600137364191 4.264884339106209 4 2.2442325588120338 2.950991071041428 5 1.294130785780233 2.127101522189636 6 0.8517164112998203 1.6799111448608097 7 0.5968294960348696 1.3733725530942156 8 0.4097496172042834 1.077577684170616 9 0.3372574421280961 0.9978013717974447 >10 2.59756499062526 17.56242772496791 >50 0.4303995644768814 9.875425478080743 >100 0.2910488858027057 17.106986036761608 >500 0.012788511648906766 2.6558969830817354 >1k 0.003086882122149909 1.4680568062697852 >5k 0.0013229494809213896 2.9684407249912503 >10k+ 4.409831603071299E-4 1.5564852304649635 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10737 1.558478882807091 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8057 1.169476050924535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6656 0.9661204660486167 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5764 0.8366463891683033 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.31076681457483296 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1675 0.24312676992659749 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1581 0.22948264074862723 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1385 0.20103317990945524 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1250 0.1814378880049235 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1066 0.15473023089059876 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.149359669405653 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 938 0.1361509911588946 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 833 0.12091020856648102 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 808 0.11728145080638255 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 714 0.1036373216284123 RNA PCR Primer, Index 39 (95% over 23bp) TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 710 0.10305672038679656 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 705 0.10233096883477685 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 700 0.10160521728275716 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.451503104039388E-4 0.0 18-19 0.0 0.0 0.0 2.903006208078776E-4 0.0 20-21 0.0 0.0 0.0 8.709018624236328E-4 0.0 22-23 0.0 0.0 0.0 0.0028304310528768067 0.0 24-25 0.0 0.0 0.0 0.007765541606610726 0.0 26-27 0.0 0.0 0.0 0.0158939589892313 0.0 28-29 0.0 0.0 0.0 0.036650453376994546 0.0 30-31 0.0 0.0 0.0 0.09064636884725978 0.0 32-33 0.0 0.0 0.0 0.16285864827321933 0.0 34-35 0.0 0.0 0.0 0.26569764319440997 0.0 36-37 0.0 0.0 0.0 0.41803289396334375 0.0 38-39 0.0 0.0 0.0 0.6271219161002175 0.0 40-41 0.0 0.0 0.0 0.8715550388204505 0.0 42-43 0.0 0.0 0.0 1.100239352861856 0.0 44-45 0.0 0.0 0.0 1.3647757935730345 0.0 46-47 0.0 0.0 0.0 1.6669061646788332 0.0 48-49 0.0 0.0 0.0 1.9959619183645625 0.0 50-51 0.0 0.0 0.0 2.336774847193011 0.0 52-53 0.0 0.0 0.0 2.6569764319441 0.0 54-55 0.0 0.0 0.0 2.995612106116489 0.0 56-57 0.0 0.0 0.0 3.3851955392406605 0.0 58-59 0.0 0.0 0.0 3.8219528232461126 0.0 60-61 0.0 0.0 0.0 4.288611071194776 0.0 62-63 0.0 0.0 0.0 4.730158315443557 0.0 64-65 0.0 0.0 0.0 5.175987493849256 0.0 66-67 0.0 0.0 0.0 5.675594862259613 0.0 68-69 0.0 0.0 0.0 6.204014567285152 0.0 70-71 0.0 0.0 0.0 6.780043574123184 0.0 72-73 0.0 0.0 0.0 7.369571559828781 0.0 74-75 0.0 0.0 0.0 7.94661661883964 0.0 76-77 0.0 0.0 0.0 8.535564003303621 0.0 78-79 0.0 0.0 0.0 9.219729991392587 0.0 80-81 0.0 0.0 0.0 9.945554118567483 0.0 82-83 0.0 0.0 0.0 10.670217043259147 0.0 84-85 0.0 0.0 0.0 11.393718765467579 0.0 86-87 0.0 0.0 0.0 12.134783675234889 0.0 88-89 0.0 0.0 0.0 12.875413134070987 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4550 0.0 39.429417 1 GTATCAA 7345 0.0 35.63373 1 CGTCACT 110 6.893606E-8 34.608894 1 AGATAGT 90 2.4136612E-5 31.662651 6 ATGCCGC 30 0.003953676 31.662651 94-95 AGGCTTA 400 0.0 30.871086 6 CAACGGA 65 0.0058460915 29.227062 9 GATAGTC 65 0.0058460915 29.227062 7 GTACATG 5805 0.0 28.691698 1 CATATAG 170 3.587047E-9 27.976213 3 GTATTAT 205 3.092282E-11 27.85594 1 GTGGTAC 225 3.6379788E-12 27.494843 1 CGACCGT 35 0.008397333 27.139414 18-19 GTTCGAC 35 0.008397333 27.139414 16-17 TCGACCG 35 0.008397333 27.139414 18-19 ATCAACG 9600 0.0 27.099043 3 TCAACGC 9635 0.0 27.0006 4 TATCAAC 9685 0.0 26.876835 2 ACATGGG 5965 0.0 26.869358 3 CAACGCA 9785 0.0 26.489485 5 >>END_MODULE