FastQCFastQC Report
Thu 26 May 2016
SRR938604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688995
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111261.6148157824077098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80881.1738837001719897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64150.9310662631804295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52840.766914128549554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.2828757828431266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18550.26923272302411483No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.23962438043817447No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15350.22278826406577695No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.16066880020900007No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.1463000457187643No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9140.13265698589975253No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8730.1267062895957155No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT7840.11378892444792778No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7610.11045072896029724No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG7090.10290350437956734No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC6980.10130697610287447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA250.004942248657.2264331
TCGACGC250.00500385857.047614
ATCGACG250.00500385857.047613
GGTATCA48550.040.5673141
GTATCAA76200.034.3583871
CATATAG1605.2750693E-1132.683533
CGTATCA601.5066689E-731.66550692-93
GTACATG60300.029.7362351
TAAGATC650.00581867629.2551842
AGGGGTC2500.028.5238065
ATGGGAT16300.028.2904825
ACATGGG60600.027.9275993
CATGGGA39850.027.67684
TACATGG64150.027.3455032
GTCCTAT700.0082285727.250681
GTCACTA1754.7493813E-927.250681
GTCCCGA350.00840256227.13595252-53
CGATATC350.00840256227.13595280-81
ATCAACG96750.026.7794573
GTGGTAC2155.4569682E-1126.6169471
Produced by FastQC (version 0.11.5)