##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938604_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688995 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.366982343848647 31.0 31.0 34.0 30.0 34.0 2 31.500933969041867 33.0 31.0 34.0 30.0 34.0 3 31.500890427361593 33.0 31.0 34.0 28.0 34.0 4 35.211722871718955 37.0 35.0 37.0 33.0 37.0 5 35.245595396193 37.0 35.0 37.0 33.0 37.0 6 35.27695411432594 37.0 35.0 37.0 33.0 37.0 7 35.23141822509597 37.0 35.0 37.0 32.0 37.0 8 35.21131648270307 37.0 35.0 37.0 32.0 37.0 9 36.71244203513814 39.0 37.0 39.0 32.0 39.0 10-11 36.56933359458341 39.0 37.0 39.0 32.0 39.0 12-13 36.4419792596463 39.0 37.0 39.0 32.0 39.0 14-15 37.500360670251595 40.0 37.0 41.0 32.0 41.0 16-17 37.48589176989673 40.0 37.0 41.0 32.0 41.0 18-19 37.624936320292605 40.0 37.0 41.0 32.0 41.0 20-21 37.647811667718926 40.0 37.0 41.0 32.0 41.0 22-23 37.50165603523973 40.0 37.0 41.0 32.0 41.0 24-25 37.36111365104246 40.0 36.5 41.0 32.0 41.0 26-27 37.14222019027714 39.0 36.0 41.0 31.5 41.0 28-29 36.92783256772546 39.0 36.0 41.0 31.0 41.0 30-31 36.570510671340145 39.0 35.0 40.0 30.5 41.0 32-33 36.154842923388415 38.0 35.0 40.0 30.0 41.0 34-35 35.84146256504039 38.0 35.0 40.0 30.0 41.0 36-37 35.517418123498715 38.0 35.0 40.0 28.5 41.0 38-39 35.250190494851196 38.0 35.0 40.0 27.0 41.0 40-41 34.95063607137933 38.0 34.0 40.0 25.5 41.0 42-43 34.48102018156881 38.0 34.0 40.0 24.0 41.0 44-45 33.886307592943346 38.0 33.0 40.0 22.0 41.0 46-47 33.238661383609454 37.5 33.0 40.0 17.0 41.0 48-49 32.65874861210894 37.0 32.5 40.0 10.5 41.0 50-51 31.91107555207222 36.5 30.5 39.5 7.5 40.5 52-53 31.96051567863337 37.0 31.0 39.0 4.0 40.0 54-55 32.0976320582878 37.0 31.5 40.0 2.0 41.0 56-57 31.83050820397826 36.5 31.0 40.0 2.0 41.0 58-59 31.505042126575667 36.0 31.0 39.0 2.0 41.0 60-61 31.085973773394585 36.0 30.5 39.0 2.0 40.5 62-63 30.488312687319937 35.0 29.0 39.0 2.0 40.0 64-65 29.954873402564605 35.0 28.0 38.5 2.0 40.0 66-67 29.389041284769846 35.0 28.0 38.0 2.0 40.0 68-69 28.701071125334728 34.0 26.0 37.5 2.0 40.0 70-71 28.01467790042018 34.0 25.5 37.0 2.0 39.0 72-73 27.250369741434987 33.5 23.5 36.0 2.0 39.0 74-75 26.44620788249552 33.0 21.5 36.0 2.0 38.5 76-77 25.61663727603248 32.0 18.5 35.0 2.0 37.5 78-79 24.727802814243937 31.0 10.5 35.0 2.0 37.0 80-81 23.85056567899622 31.0 4.5 34.5 2.0 36.0 82-83 22.941205669126774 30.0 2.0 34.0 2.0 36.0 84-85 22.000918003759097 29.0 2.0 34.0 2.0 35.0 86-87 21.1036466157229 28.5 2.0 34.0 2.0 35.0 88-89 20.092831588037647 26.5 2.0 33.5 2.0 35.0 90-91 18.96612239566325 25.5 2.0 33.0 2.0 35.0 92-93 17.814450032293415 23.0 2.0 32.0 2.0 34.0 94-95 16.535440750658566 15.0 2.0 31.5 2.0 34.0 96-97 15.026637348601948 2.0 2.0 31.0 2.0 34.0 98-99 13.274128984970863 2.0 2.0 30.5 2.0 34.0 100-101 9.384137765876385 2.0 2.0 21.5 2.0 29.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5360199862295261 1101 2 0.6373562109144757 1101 3 0.6044842339285807 1101 4 0.4030357069725454 1101 5 0.3950652111330797 1101 6 0.4884375224324131 1101 7 0.4652706323481084 1101 8 0.6367305649434556 1101 9 0.5417778744231825 1101 10-11 0.6774334872363994 1101 12-13 0.6173021185941963 1101 14-15 0.5938342324718917 1101 16-17 0.807980466827793 1101 18-19 0.8297172629840119 1101 20-21 1.0525527809394148 1101 22-23 1.0456244110084825 1101 24-25 1.04684646222222 1101 26-27 1.1432841095947097 1101 28-29 1.0097674001821986 1101 30-31 0.9867832353345136 1101 32-33 0.9916492699955839 1101 34-35 1.2407176892653737 1101 36-37 1.1844899587222315 1101 38-39 1.105956505030484 1101 40-41 1.0046783719582848 1101 42-43 0.9581472742130899 1101 44-45 0.6063111800421481 1101 46-47 0.5102217762547454 1101 48-49 0.20536683656334986 1101 50-51 0.24140300862042707 1101 52-53 0.2255833196055832 1101 54-55 0.5039039264235043 1101 56-57 0.7444740595488035 1101 58-59 0.5567452347943522 1101 60-61 0.7490201550381066 1101 62-63 0.8981985504926691 1101 64-65 0.8380680765015924 1101 66-67 1.0480344854005637 1101 68-69 1.2689531578062336 1101 70-71 1.6752881774299162 1101 72-73 1.4925040598417851 1101 74-75 1.6331372131575108 1101 76-77 1.5005062578630408 1101 78-79 1.56486134406072 1101 80-81 1.9407763791841823 1101 82-83 1.9209830655295441 1101 84-85 2.039514103438261 1101 86-87 2.1486168876321443 1101 88-89 2.3574698206050506 1101 90-91 2.3284369748859994 1101 92-93 2.445012153312625 1101 94-95 2.3721083115395842 1101 96-97 2.4891061237714993 1101 98-99 2.6262777484163013 1101 100-101 2.360187579174136 1102 1 0.5733716552990629 1102 2 0.580143292106797 1102 3 0.37064691429776175 1102 4 0.4433077856387655 1102 5 0.22586875505628257 1102 6 0.4351086251642897 1102 7 0.5090176287622867 1102 8 0.5963944097797551 1102 9 0.6395462871744044 1102 10-11 0.6862156641121828 1102 12-13 0.6855361949684493 1102 14-15 0.6623541886041409 1102 16-17 0.8336529619912554 1102 18-19 0.6674238172703681 1102 20-21 0.45650211121679973 1102 22-23 0.35015782320519406 1102 24-25 0.6032239220819946 1102 26-27 0.35006882488558944 1102 28-29 0.4520849978064376 1102 30-31 0.607911228138228 1102 32-33 0.6109868175908773 1102 34-35 0.8928106494763313 1102 36-37 0.9067368257511745 1102 38-39 0.9416397206848686 1102 40-41 1.0576807255373168 1102 42-43 1.0520255042230673 1102 44-45 1.261129855876682 1102 46-47 1.3698314025162546 1102 48-49 1.2891998433529963 1102 50-51 1.2900617150145202 1102 52-53 1.2161172493432417 1102 54-55 1.1791272699844342 1102 56-57 1.2595197140532584 1102 58-59 1.4284345130711849 1102 60-61 1.2397376885858513 1102 62-63 1.531121547794065 1102 64-65 1.7215807008038375 1102 66-67 1.997788653453501 1102 68-69 1.8708335565592051 1102 70-71 1.6595573972985598 1102 72-73 1.5827533420617854 1102 74-75 1.5710273712539475 1102 76-77 1.8103752707153085 1102 78-79 2.1288269399105353 1102 80-81 2.299161158551552 1102 82-83 2.5939206032669055 1102 84-85 2.399278991982122 1102 86-87 2.5419911313446377 1102 88-89 2.601294448160413 1102 90-91 2.273544364631295 1102 92-93 2.526964761349298 1102 94-95 3.0110606608057857 1102 96-97 2.796976442798524 1102 98-99 2.6034086000175964 1102 100-101 2.176248599715583 1103 1 0.5657185395403417 1103 2 0.4291830897339004 1103 3 0.444484517176587 1103 4 0.39031079035367355 1103 5 0.26480653489262806 1103 6 0.34693390039854677 1103 7 0.3209628550660142 1103 8 0.286324458116475 1103 9 0.2529428026552196 1103 10-11 0.5794544617511619 1103 12-13 0.6211153448597813 1103 14-15 0.8607105026621653 1103 16-17 0.5201792502776144 1103 18-19 0.6394277127110328 1103 20-21 0.693507609963838 1103 22-23 0.687747638052997 1103 24-25 0.7990363233598927 1103 26-27 0.8855921559624136 1103 28-29 0.6353631061424423 1103 30-31 0.6308290082120962 1103 32-33 0.7855013720498434 1103 34-35 0.7867596524572917 1103 36-37 1.0739913714216485 1103 38-39 0.9612309369412131 1103 40-41 1.151829251089275 1103 42-43 1.3342014878812165 1103 44-45 1.1977755014153288 1103 46-47 1.2868240748123085 1103 48-49 0.8540791203212095 1103 50-51 1.0153250729318906 1103 52-53 1.3063762723952266 1103 54-55 1.3069191014353692 1103 56-57 1.2635932998776518 1103 58-59 1.1266756265971516 1103 60-61 1.2480641930216656 1103 62-63 1.2602850969205583 1103 64-65 1.2699186065294228 1103 66-67 1.2802164155940936 1103 68-69 1.2076193429512934 1103 70-71 1.5401965821063435 1103 72-73 1.6081152736302968 1103 74-75 1.5451108144434542 1103 76-77 1.571243906479726 1103 78-79 1.6701269244712869 1103 80-81 1.6686104525029286 1103 82-83 1.8743887687281209 1103 84-85 2.0935047137668974 1103 86-87 2.667838805362763 1103 88-89 2.7134700755282566 1103 90-91 2.672540813604588 1103 92-93 3.0426902277926864 1103 94-95 2.658822563871073 1103 96-97 3.1769101728017333 1103 98-99 2.637788239214281 1103 100-101 2.0801084113567736 1104 1 0.3829301847209088 1104 2 0.4263657073382703 1104 3 0.21636191835476382 1104 4 0.1865485888112275 1104 5 0.12220145222095624 1104 6 0.3139790638921056 1104 7 0.11296035072610522 1104 8 0.2675988256283546 1104 9 0.3000699263639177 1104 10-11 0.33279121009346113 1104 12-13 0.4727332053566613 1104 14-15 0.5323289323272036 1104 16-17 0.47739203379450856 1104 18-19 0.2422052533354062 1104 20-21 0.20625697676425858 1104 22-23 0.2949220027190407 1104 24-25 0.3676068575242013 1104 26-27 0.2828288445420526 1104 28-29 0.3938081387273229 1104 30-31 0.186052549007222 1104 32-33 0.3367265065158236 1104 34-35 0.18014022674796593 1104 36-37 0.1800131705622121 1104 38-39 0.34218941612388676 1104 40-41 0.4133051041300533 1104 42-43 0.35740931963511 1104 44-45 0.38370851032281195 1104 46-47 0.2708873663117828 1104 48-49 0.23849529608033038 1104 50-51 0.20115847741124426 1104 52-53 0.451471465770112 1104 54-55 0.6104564815543299 1104 56-57 0.6429865666183154 1104 58-59 0.6495846450666569 1104 60-61 0.6966683081074656 1104 62-63 0.553022511189603 1104 64-65 0.44890776387596176 1104 66-67 0.6544587104801174 1104 68-69 0.9809623521433544 1104 70-71 0.5197919035440641 1104 72-73 0.9404667918863652 1104 74-75 0.8406798403121485 1104 76-77 0.8356509755482833 1104 78-79 1.358436210449831 1104 80-81 1.2765105065739064 1104 82-83 1.4540865404347727 1104 84-85 1.625676944052337 1104 86-87 1.7877198492171686 1104 88-89 2.2121752179625886 1104 90-91 1.8334588818478466 1104 92-93 2.5506016652268784 1104 94-95 2.3649468132805573 1104 96-97 2.7732561132098255 1104 98-99 2.85962152986 1104 100-101 2.61783003120936 1105 1 0.44095454407163004 1105 2 0.38221258790026624 1105 3 0.5214203943454265 1105 4 0.45008367132632543 1105 5 0.38866597966063665 1105 6 0.4314155224456897 1105 7 0.4323868610735957 1105 8 0.31626209958585605 1105 9 0.31913287350457864 1105 10-11 0.4117989760991634 1105 12-13 0.3449020415643531 1105 14-15 0.4535613520651367 1105 16-17 0.43933183134050324 1105 18-19 0.45624872900787494 1105 20-21 0.4286022571143988 1105 22-23 0.4438730889949909 1105 24-25 0.5389331960465071 1105 26-27 0.48806345674471885 1105 28-29 0.671268862199625 1105 30-31 0.4293904739255723 1105 32-33 0.5683836033886891 1105 34-35 0.5584779361150041 1105 36-37 0.7493344713425074 1105 38-39 0.5688980708168501 1105 40-41 0.9620186733928477 1105 42-43 0.993627482193645 1105 44-45 1.0550132937142749 1105 46-47 0.9267904955009172 1105 48-49 0.8326973764583911 1105 50-51 0.8690257306511775 1105 52-53 1.0115610119422271 1105 54-55 1.2230892252350856 1105 56-57 1.143458726627184 1105 58-59 1.2040072446975536 1105 60-61 1.216069978732751 1105 62-63 1.3467080895753512 1105 64-65 1.505651132593922 1105 66-67 1.5387045785668185 1105 68-69 1.5683889363941539 1105 70-71 1.4381082220098271 1105 72-73 1.6470429543751024 1105 74-75 1.7901043183187326 1105 76-77 1.7635387985416564 1105 78-79 2.072628519168571 1105 80-81 2.1030625273161014 1105 82-83 2.453711891732084 1105 84-85 2.756399574681563 1105 86-87 3.2554415540022035 1105 88-89 3.029199870873658 1105 90-91 2.4904818514058427 1105 92-93 2.924250620418892 1105 94-95 3.4916406873123726 1105 96-97 3.2437594563079735 1105 98-99 3.0263802335139047 1105 100-101 2.350458135404809 1106 1 0.07497398997226412 1106 2 0.20557140729868806 1106 3 0.01462026378292336 1106 4 0.1780250368914551 1106 5 0.21793967626106792 1106 6 -0.1879077285607238 1106 7 -0.005788824626840494 1106 8 0.005479812565944542 1106 9 0.3465668256910064 1106 10-11 0.22154962149338076 1106 12-13 0.2617159207544688 1106 14-15 0.26279469298164315 1106 16-17 -0.3119337347765452 1106 18-19 0.24659897282347032 1106 20-21 0.13556163437080926 1106 22-23 0.14744872128349584 1106 24-25 0.2332883458472068 1106 26-27 0.20711964807814098 1106 28-29 0.49289772838294255 1106 30-31 0.3813157763459216 1106 32-33 0.2275828200136445 1106 34-35 0.04421200402729397 1106 36-37 0.03916345424079992 1106 38-39 -0.06609663744519167 1106 40-41 0.4592610349916484 1106 42-43 0.07892930907962636 1106 44-45 0.23374149620484985 1106 46-47 0.5526131876602491 1106 48-49 0.04110777793810172 1106 50-51 0.02662357834804041 1106 52-53 0.22797232340304419 1106 54-55 -0.17871095478292176 1106 56-57 0.40527710626734503 1106 58-59 0.553345035619504 1106 60-61 0.5937655504877277 1106 62-63 0.6083860156097103 1106 64-65 0.3610333741147791 1106 66-67 0.6189659014024009 1106 68-69 0.541418876750825 1106 70-71 0.4812907820240717 1106 72-73 0.6892067311923427 1106 74-75 0.944123566397387 1106 76-77 1.0507585095237424 1106 78-79 1.6945887371683952 1106 80-81 1.366073773495664 1106 82-83 1.7307853133599629 1106 84-85 1.992572312834497 1106 86-87 1.7967843696024417 1106 88-89 1.6487367696184805 1106 90-91 1.9798766150580356 1106 92-93 2.1453503128057143 1106 94-95 2.2651711172784452 1106 96-97 1.6213363348749539 1106 98-99 1.6485382734937275 1106 100-101 1.06259847031742 1107 1 0.1655823063870301 1107 2 0.008885566258008737 1107 3 0.23153506940740343 1107 4 -0.13666215763445422 1107 5 0.02381981762507479 1107 6 0.012195662671693697 1107 7 0.16290806987650086 1107 8 -0.009635985614366405 1107 9 0.28045095231706796 1107 10-11 0.14213387594860194 1107 12-13 0.1803037845036215 1107 14-15 0.2304040997039607 1107 16-17 0.038251429521530156 1107 18-19 0.10858671626282757 1107 20-21 0.23473638435201138 1107 22-23 0.1646549021993593 1107 24-25 0.16278492453732696 1107 26-27 0.33704389050237893 1107 28-29 0.24749393862431646 1107 30-31 0.296020829357424 1107 32-33 0.1435877827927996 1107 34-35 0.08856778265726462 1107 36-37 0.09029981787715968 1107 38-39 0.17175677258564548 1107 40-41 -0.021004398499592014 1107 42-43 -0.17150023449507756 1107 44-45 -0.5538148990128278 1107 46-47 -0.4803601562001987 1107 48-49 -0.5083649267765438 1107 50-51 -0.41327415832389036 1107 52-53 -0.29654361764515613 1107 54-55 -0.2220760574222176 1107 56-57 -0.2851526252914205 1107 58-59 -0.15927715401881315 1107 60-61 -0.04687754297794555 1107 62-63 0.017774879434060864 1107 64-65 -0.015663182017259913 1107 66-67 3.667586097115816E-4 1107 68-69 0.30713340415890045 1107 70-71 0.46757640500324626 1107 72-73 0.3993014751580546 1107 74-75 0.5938810669613375 1107 76-77 0.9082628331570994 1107 78-79 1.0756455835478214 1107 80-81 1.2145313642919646 1107 82-83 1.5773640034870375 1107 84-85 1.7057358965625973 1107 86-87 1.286477203650115 1107 88-89 1.4822994435037344 1107 90-91 1.5352463797019915 1107 92-93 1.782481864050169 1107 94-95 1.7408422203689078 1107 96-97 1.5078381395219154 1107 98-99 1.294389465312662 1107 100-101 0.9267640090797347 1108 1 0.27931486186632526 1108 2 0.18533260354924508 1108 3 0.22083957638521312 1108 4 0.07766236764580725 1108 5 0.06883240055804407 1108 6 0.2230169850235626 1108 7 0.13346686649055073 1108 8 0.22759519718132992 1108 9 0.29896788208142766 1108 10-11 0.259991631610923 1108 12-13 0.20003611120232279 1108 14-15 0.2774614092441112 1108 16-17 0.30959951920351614 1108 18-19 0.35244538704754547 1108 20-21 0.31721893983129945 1108 22-23 0.2840569269076596 1108 24-25 0.42604704990909426 1108 26-27 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60-61 -0.3901931737148985 2316 62-63 -0.2632866206970732 2316 64-65 -0.476810353972855 2316 66-67 -0.08926083121457395 2316 68-69 -0.4279341279680331 2316 70-71 -0.6194558967713881 2316 72-73 -0.8697998448874031 2316 74-75 -0.594323758635305 2316 76-77 -0.510305223491212 2316 78-79 -0.6418450187775555 2316 80-81 -0.8193385106971114 2316 82-83 -0.6466721884877842 2316 84-85 -0.12736249490518503 2316 86-87 -0.11627871907040088 2316 88-89 -0.8120881777918321 2316 90-91 -1.1643394669629252 2316 92-93 -1.0808857817230617 2316 94-95 -0.8437587062855219 2316 96-97 -0.5269059377575651 2316 98-99 -0.7009007698477561 2316 100-101 -0.4110441472276367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3455.0 3 713.0 4 589.0 5 538.0 6 654.0 7 835.0 8 1033.0 9 1456.0 10 2006.0 11 3035.0 12 4526.0 13 6332.0 14 7456.0 15 9476.0 16 12115.0 17 14829.0 18 12330.0 19 9249.0 20 9471.0 21 10316.0 22 11691.0 23 13087.0 24 14571.0 25 16386.0 26 18751.0 27 20997.0 28 23933.0 29 27061.0 30 31099.0 31 35862.0 32 41795.0 33 48965.0 34 59010.0 35 66429.0 36 69568.0 37 60167.0 38 18890.0 39 319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.701778056886745 22.086197073921298 15.926016048118127 19.28600882107383 2 14.61125226860254 23.754773139745915 41.281451905626135 20.35252268602541 3 17.07222871579681 32.11546140275731 30.732451013844624 20.079858867601256 4 10.785210613136313 19.573052438372716 40.31054692037824 29.33119002811273 5 10.494064745327975 41.49202702055008 34.20385671730236 13.81005151681958 6 25.35290017136716 41.26419588137907 18.805658020854512 14.577245926399257 7 24.467227813198317 36.8811791337125 23.394931676513593 15.256661376575591 8 25.249847180498342 34.910508028650206 21.32648779404142 18.51315699681003 9 27.945406366837272 17.242440741950706 22.120730335039383 32.69142255617264 10-11 22.508265488807286 29.968114161755288 27.868164546026104 19.65545580341132 12-13 25.12841918810616 28.75595272663918 28.333091352575646 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17.557409818788354 30.15207668288179 34.56717808911206 17.723335409217796 40-41 17.517207141099643 29.78492071614223 35.05118049371049 17.64669164904763 42-43 17.421529952712927 29.815170331444996 34.090095844326456 18.673203871515618 44-45 17.376173236987338 30.010044911787677 33.8738521656075 18.739929685617486 46-47 18.077725968852707 30.100558026641274 33.05531199554043 18.76640400896559 48-49 17.81643693036199 30.47659970429813 32.36505608101389 19.341907284325988 50-51 18.26661789297659 30.278735832404312 32.12429742660721 19.33034884801189 52-53 17.969594283196873 30.524962023699537 32.309866290036595 19.19557740306699 54-55 18.004148528346583 30.485296529780715 32.16996482895667 19.340590112916033 56-57 18.1409236593353 30.640197877232506 31.3556738741668 19.863204589265393 58-59 17.840344581085052 29.780209811271458 32.298380512444616 20.081065095198873 60-61 18.403097568321776 30.540223753588002 31.542361133791292 19.51431754429893 62-63 18.493965454047963 30.293427937091792 32.21233298761737 19.00027362124288 64-65 18.391371022934692 29.699993250446887 32.28195133209326 19.626684394525167 66-67 18.066790234018374 30.10734974156812 31.668246335434556 20.15761368897894 68-69 18.326171317924537 29.96659338936664 31.98093290300761 19.726302389701214 70-71 18.57497367097391 29.45555711203064 31.895610038635258 20.07385917836019 72-73 18.1484314781455 29.574450171796364 31.51523377224752 20.761884577810616 74-75 18.0359054569472 29.978552531781084 31.413609525433937 20.571932485837777 76-77 17.82288229940648 29.31302009019614 32.40630937537649 20.457788235020892 78-79 17.84460247025404 28.913062096014468 32.13151947186672 21.11081596186478 80-81 17.94599161140437 29.881414376157167 31.223604192991406 20.948989819447057 82-83 18.375890647987465 28.997908968644694 31.286358070793135 21.33984231257471 84-85 18.16978370065442 28.519971927461373 31.39276224925773 21.91748212262648 86-87 18.095833188188713 28.936467296013745 31.495584700595675 21.472114815201866 88-89 18.44342584225215 28.088886953302776 31.756798443788764 21.710888760656307 90-91 18.41718617974233 28.220884280458662 31.519586635036134 21.842342904762873 92-93 18.68439732269223 28.262116036992253 31.304933510039906 21.748553130275614 94-95 18.32075863400584 27.38797097051902 31.652165959899364 22.639104435575778 96-97 18.801699683813517 27.195158775554052 31.162515206856806 22.840626333775628 98-99 18.693024781679675 27.195687012263097 31.014695977331847 23.09659222872538 100-101 19.352398640621356 26.25327255803409 31.67763514038847 22.716693660956086 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3019.0 1 2073.0 2 1362.0 3 1535.0 4 1247.5 5 1197.0 6 1934.0 7 3681.5 8 5485.0 9 5770.0 10 4468.0 11 3233.0 12 2883.0 13 2758.0 14 2683.0 15 2579.5 16 2404.0 17 2290.5 18 2318.5 19 2395.5 20 2484.0 21 2615.0 22 2888.0 23 3257.0 24 3564.5 25 4023.5 26 4814.5 27 5672.5 28 6324.0 29 7596.0 30 9889.0 31 12243.5 32 15588.0 33 19890.5 34 23257.0 35 26610.5 36 32112.0 37 35974.0 38 35907.0 39 35043.5 40 32730.0 41 29533.5 42 26332.5 43 24836.0 44 24638.0 45 23630.5 46 21531.5 47 19190.5 48 18653.5 49 17356.5 50 14234.5 51 11199.5 52 9194.5 53 8350.5 54 7961.0 55 7440.0 56 6806.5 57 6289.0 58 6014.0 59 5631.5 60 4998.5 61 4533.5 62 3934.5 63 2685.0 64 1706.0 65 1307.5 66 990.5 67 757.5 68 575.0 69 453.5 70 387.5 71 320.5 72 266.0 73 249.5 74 219.0 75 153.5 76 112.0 77 81.0 78 47.0 79 29.5 80 24.0 81 14.5 82 5.0 83 4.0 84 4.5 85 5.0 86 4.0 87 3.5 88 3.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3895528995130589 2 0.035559038889977426 3 0.040638901588545634 4 0.049202098708989186 5 0.06995696630599642 6 0.060232657711594424 7 0.04150973519401447 8 0.0397680679830768 9 0.03918751224609758 10-11 0.04180001306250408 12-13 0.03410764954752937 14-15 0.0322934128694693 16-17 0.027503828039390707 18-19 0.031640287665367674 20-21 0.03918751224609758 22-23 0.03809897023926154 24-25 0.04738786203092911 26-27 0.04078404052279044 28-29 0.03628473356120146 30-31 0.03955035958170959 32-33 0.033889941146162166 34-35 0.03497848315299821 36-37 0.037300706100915106 38-39 0.019158339320314372 40-41 0.015892713299806238 42-43 0.017053824773764686 44-45 0.012917365147787721 46-47 0.02017431186002801 48-49 0.018214936247723135 50-51 0.014368754490235778 52-53 0.011393406338217258 54-55 0.011030559002605245 56-57 0.012046531542318885 58-59 0.006168404705404248 60-61 0.009796878061524395 62-63 0.012554517812175706 64-65 0.008780905521810753 66-67 0.004644445895833787 68-69 0.008635766587565947 70-71 0.01690868583951988 72-73 0.014223615555990972 74-75 0.014876740760092598 76-77 0.007547224580729903 78-79 0.011320836871094855 80-81 0.012119101009441288 82-83 0.014586462891602987 84-85 0.009506600193034783 86-87 0.003918751224609758 88-89 0.02068229812988483 90-91 0.03033403725716442 92-93 0.01364305981901175 94-95 0.025834730295575443 96-97 0.02336736841341374 98-99 0.014368754490235778 100-101 0.1286656652080204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 688995.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.75044312957778 #Duplication Level Percentage of deduplicated Percentage of total 1 76.63919795015104 26.632460898631784 2 10.86757108140274 7.553058216418602 3 4.079874912599069 4.253323833781954 4 2.111122274053315 2.9344973809629864 5 1.2088187037712517 2.100349280968641 6 0.7492714918244314 1.5622509819155286 7 0.5574849383695804 1.3560994052485797 8 0.4109965995985687 1.1425851168639933 9 0.3028569002309855 0.9471970339089367 >10 2.389433813440767 17.10257688095155 >50 0.3999608915388619 9.64496397765914 >100 0.26915607162493893 16.713401746611996 >500 0.010061909219623884 2.227016092353586 >1k 0.002515477304905971 1.326506706848057 >5k 0.0012577386524529855 2.882766414981055 >10k+ 4.192462174843285E-4 1.620946031893628 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11126 1.6148157824077098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8088 1.1738837001719897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6415 0.9310662631804295 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5284 0.766914128549554 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1949 0.2828757828431266 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1855 0.26923272302411483 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1651 0.23962438043817447 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1535 0.22278826406577695 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1107 0.16066880020900007 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.1463000457187643 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 914 0.13265698589975253 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 873 0.1267062895957155 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 784 0.11378892444792778 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 761 0.11045072896029724 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 709 0.10290350437956734 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 698 0.10130697610287447 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.1770840136720878E-4 0.0 20-21 0.0 0.0 0.0 8.708336054688351E-4 0.0 22-23 0.0 0.0 0.0 0.002757639750651311 0.0 24-25 0.0 0.0 0.0 0.008853474988933158 0.0 26-27 0.0 0.0 0.0 0.01908576985319197 0.0 28-29 0.0 0.0 0.0 0.03773612290364952 0.0 30-31 0.0 0.0 0.0 0.09005870869890202 0.0 32-33 0.0 0.0 0.0 0.1641521346308754 0.0 34-35 0.0 0.0 0.0 0.26582195806936193 0.0 36-37 0.0 0.0 0.0 0.4084209609648837 0.0 38-39 0.0 0.0 0.0 0.6216300553705034 0.0 40-41 0.0 0.0 0.0 0.8565374204457217 0.0 42-43 0.0 0.0 0.0 1.0759149195567457 0.0 44-45 0.0 0.0 0.0 1.3262070116619133 0.0 46-47 0.0 0.0 0.0 1.631071343043128 0.0 48-49 0.0 0.0 0.0 1.9593030428377562 0.0 50-51 0.0 0.0 0.0 2.287099325829651 0.0 52-53 0.0 0.0 0.0 2.6135167889462187 0.0 54-55 0.0 0.0 0.0 2.942982169681928 0.0 56-57 0.0 0.0 0.0 3.3182388841718735 0.0 58-59 0.0 0.0 0.0 3.743713669910522 0.0 60-61 0.0 0.0 0.0 4.200828743314538 0.0 62-63 0.0 0.0 0.0 4.633850753633916 0.0 64-65 0.0 0.0 0.0 5.0798626985682045 0.0 66-67 0.0 0.0 0.0 5.5773263956922765 0.0 68-69 0.0 0.0 0.0 6.10534183847488 0.0 70-71 0.0 0.0 0.0 6.6755927111227225 0.0 72-73 0.0 0.0 0.0 7.254261641956763 0.0 74-75 0.0 0.0 0.0 7.823278833663524 0.0 76-77 0.0 0.0 0.0 8.401367208760586 0.0 78-79 0.0 0.0 0.0 9.05137192577595 0.0 80-81 0.0 0.0 0.0 9.729678734969049 0.0 82-83 0.0 0.0 0.0 10.427506730818076 0.0 84-85 0.0 0.0 0.0 11.125334726667102 0.0 86-87 0.0 0.0 0.0 11.822654736246271 0.0 88-89 0.0 0.0 0.0 12.530860165893802 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCGA 25 0.0049422486 57.226433 1 TCGACGC 25 0.005003858 57.04761 4 ATCGACG 25 0.005003858 57.04761 3 GGTATCA 4855 0.0 40.567314 1 GTATCAA 7620 0.0 34.358387 1 CATATAG 160 5.2750693E-11 32.68353 3 CGTATCA 60 1.5066689E-7 31.665506 92-93 GTACATG 6030 0.0 29.736235 1 TAAGATC 65 0.005818676 29.255184 2 AGGGGTC 250 0.0 28.523806 5 ATGGGAT 1630 0.0 28.290482 5 ACATGGG 6060 0.0 27.927599 3 CATGGGA 3985 0.0 27.6768 4 TACATGG 6415 0.0 27.345503 2 GTCCTAT 70 0.00822857 27.25068 1 GTCACTA 175 4.7493813E-9 27.25068 1 GTCCCGA 35 0.008402562 27.135952 52-53 CGATATC 35 0.008402562 27.135952 80-81 ATCAACG 9675 0.0 26.779457 3 GTGGTAC 215 5.4569682E-11 26.616947 1 >>END_MODULE