FastQCFastQC Report
Thu 26 May 2016
SRR938604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688995
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102981.4946407448530106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80951.1748996727117034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66560.9660447463334276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57060.8281627588008621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.307259123796254No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16380.23773757429299194No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.2330931283971582No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.2031945079427282No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12510.1815688067402521No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11080.16081393914324488No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.15036393587761884No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8960.13004448508334604No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT8540.12394864984506418No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8020.11640142526433428No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7770.11277295190821415No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7720.1120472572369901No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC7240.10508058839323942No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG6940.10072642036589524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT1200.047.5196761
GGTATCA46850.040.673191
GTGGTAC2050.034.7704931
GTATCAA75100.034.231881
GCCGGTG300.00395163731.66598792-93
CATTCCG600.003950277331.6659879
GGTACTC2300.030.9775983
GTCACTA1901.2732926E-1130.0015352
ACGGAGG1652.6448106E-928.7872628
CTACACG1652.6448106E-928.7872624
TGGTACT2500.028.5014572
GTACATG59900.028.321411
CATGGGG17100.028.0549554
ACATGGG60600.026.9631183
ATGGGGA8050.026.5522255
GGCATTC908.94501E-426.3883277
TACACGG1806.690243E-926.3883275
ATCAACG96850.026.38563
TCAACGC97200.026.1928544
TACATGG64250.026.0246772
Produced by FastQC (version 0.11.5)