##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938604_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688995 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.756363979419298 31.0 31.0 34.0 30.0 34.0 2 31.981822799875182 31.0 31.0 34.0 30.0 34.0 3 31.99455148440845 33.0 31.0 34.0 30.0 34.0 4 35.698637871102115 37.0 35.0 37.0 33.0 37.0 5 35.62239493755397 37.0 35.0 37.0 33.0 37.0 6 35.67286845332695 37.0 35.0 37.0 33.0 37.0 7 35.60175472971502 37.0 35.0 37.0 33.0 37.0 8 35.591264087547806 37.0 35.0 37.0 33.0 37.0 9 37.072251612856405 39.0 37.0 39.0 33.0 39.0 10-11 37.011060312484126 39.0 37.0 39.0 33.0 39.0 12-13 36.909487006436905 39.0 37.0 39.0 33.0 39.0 14-15 37.75031604002932 39.5 37.0 41.0 32.0 41.0 16-17 37.872076720440646 39.5 37.0 41.0 32.5 41.0 18-19 38.12416563255176 40.0 37.0 41.0 33.0 41.0 20-21 38.14626303529053 40.0 37.0 41.0 33.0 41.0 22-23 37.987975964992486 40.0 37.0 41.0 33.0 41.0 24-25 37.84537768779164 40.0 37.0 41.0 33.0 41.0 26-27 37.590836653386454 40.0 36.0 41.0 32.0 41.0 28-29 37.41057409705441 39.0 36.0 41.0 32.0 41.0 30-31 37.033856559191285 39.0 35.5 40.5 31.5 41.0 32-33 36.69599706819353 39.0 35.0 40.0 31.0 41.0 34-35 36.45715426091626 38.0 35.0 40.0 30.0 41.0 36-37 36.23713452202121 38.0 35.0 40.0 30.0 41.0 38-39 35.96067823423972 38.0 35.0 40.0 30.0 41.0 40-41 35.66720222933403 38.0 35.0 40.0 29.5 41.0 42-43 35.29503334567014 38.0 35.0 40.0 27.5 41.0 44-45 34.816402876653676 38.0 34.0 40.0 25.5 41.0 46-47 34.165480155879216 38.0 33.0 40.0 23.5 41.0 48-49 33.60106096560933 38.0 33.0 40.0 19.5 41.0 50-51 33.18971690650875 37.5 33.0 40.0 16.0 41.0 52-53 32.637723060399566 37.0 32.0 40.0 10.5 41.0 54-55 32.26963911204 37.0 31.0 40.0 8.0 41.0 56-57 31.92086807596572 36.0 31.0 39.0 6.0 40.5 58-59 31.455121590142163 36.0 30.5 39.0 2.0 40.0 60-61 31.058924230219375 35.0 30.0 39.0 2.0 40.0 62-63 30.64260408275822 35.0 29.0 39.0 2.0 40.0 64-65 30.053935079354712 35.0 28.0 38.0 2.0 40.0 66-67 29.455027249834906 34.5 28.0 38.0 2.0 40.0 68-69 28.812458000420904 34.0 26.0 37.5 2.0 39.5 70-71 28.1198245270285 33.5 26.0 36.5 2.0 39.0 72-73 27.597370082511482 33.0 25.0 36.0 2.0 39.0 74-75 26.952683981741522 32.5 23.5 35.5 2.0 38.5 76-77 25.768176837277483 31.0 22.0 34.5 2.0 36.5 78-79 26.271247251431433 32.0 22.5 35.0 2.0 37.0 80-81 25.91310822284632 32.0 21.5 35.0 2.0 36.5 82-83 25.29454422746174 31.5 19.0 35.0 2.0 36.0 84-85 24.561253710114006 31.0 14.5 34.0 2.0 35.5 86-87 23.77426976973708 31.0 7.0 34.0 2.0 35.0 88-89 22.890509365089734 30.0 2.0 34.0 2.0 35.0 90-91 22.116975449749273 29.0 2.0 34.0 2.0 35.0 92-93 21.158644837770957 29.0 2.0 34.0 2.0 35.0 94-95 20.109759867633294 27.5 2.0 33.0 2.0 35.0 96-97 18.84760919890565 25.5 2.0 33.0 2.0 34.5 98-99 17.217296932488622 21.0 2.0 32.5 2.0 34.0 100-101 12.990333746979296 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41205533087172697 1101 2 0.280078857568796 1101 3 0.2696712742420786 1101 4 0.19484188016174642 1101 5 0.15810235224513036 1101 6 0.15699986718674808 1101 7 0.24650593426147793 1101 8 0.30811959915823195 1101 9 0.49676642209533384 1101 10-11 0.4290310417820322 1101 12-13 0.3486710645982427 1101 14-15 0.4550761414238522 1101 16-17 0.40551522326049394 1101 18-19 0.5636200108122651 1101 20-21 0.4779509040779999 1101 22-23 0.6073162745979275 1101 24-25 0.6202400089743776 1101 26-27 0.4516353334948988 1101 28-29 0.22917257225901722 1101 30-31 0.322020286545353 1101 32-33 0.5016921537214216 1101 34-35 0.36139516502144886 1101 36-37 0.29914284263339397 1101 38-39 0.32396984536861595 1101 40-41 0.4253727435001835 1101 42-43 0.569021084025735 1101 44-45 0.6887702857671201 1101 46-47 0.5285014102967693 1101 48-49 0.5629515037273762 1101 50-51 0.5159828174484886 1101 52-53 0.6949232406827051 1101 54-55 0.8564615634409094 1101 56-57 0.737168648476672 1101 58-59 0.756862716861022 1101 60-61 0.7973576950914207 1101 62-63 0.9603043385958436 1101 64-65 0.8250404324819307 1101 66-67 1.1539856265720552 1101 68-69 1.2676913223851365 1101 70-71 1.1959295445555647 1101 72-73 1.3488162668560228 1101 74-75 1.4303275239412976 1101 76-77 1.4167936784222874 1101 78-79 1.3927257589595783 1101 80-81 1.438766312199796 1101 82-83 1.650459054732945 1101 84-85 2.1510474198560843 1101 86-87 1.8297701890512563 1101 88-89 1.5198790493901733 1101 90-91 2.058196661625729 1101 92-93 2.366231045370558 1101 94-95 2.760683615725309 1101 96-97 2.6536254409645395 1101 98-99 2.524732335786915 1101 100-101 2.2262967744823303 1102 1 0.15187024837323548 1102 2 0.1165482425576414 1102 3 0.21638673762605265 1102 4 0.20683897207455715 1102 5 0.1709546189512281 1102 6 0.16234286128432274 1102 7 0.09293182116645937 1102 8 0.1642703267961494 1102 9 0.26738241903937876 1102 10-11 0.22257903138191892 1102 12-13 0.16780282805739688 1102 14-15 0.35892196351299077 1102 16-17 0.27514419569528314 1102 18-19 0.3365994447210525 1102 20-21 0.31035389595754737 1102 22-23 0.38680070524127785 1102 24-25 0.1705844201483302 1102 26-27 0.31820588541534534 1102 28-29 0.42870678541194707 1102 30-31 0.3583627507795839 1102 32-33 0.41570026186283116 1102 34-35 0.3730423006787902 1102 36-37 0.6493899068207938 1102 38-39 0.5740370024669303 1102 40-41 0.4394042642079796 1102 42-43 0.46280936049117827 1102 44-45 0.607062893557341 1102 46-47 0.6897240391582997 1102 48-49 0.5823136961293827 1102 50-51 0.6355224524589076 1102 52-53 0.6154240231553203 1102 54-55 0.7536779325215335 1102 56-57 0.7990505259180516 1102 58-59 0.8693699562729904 1102 60-61 0.8368300497794792 1102 62-63 0.8557438171117333 1102 64-65 1.058435377832069 1102 66-67 1.0540657221939043 1102 68-69 1.3596460252303793 1102 70-71 1.809038117251582 1102 72-73 1.5935059271273602 1102 74-75 1.4487076207953855 1102 76-77 1.65592963149858 1102 78-79 1.6665123102885495 1102 80-81 1.7410484213023523 1102 82-83 1.7313593295225367 1102 84-85 1.678658229068315 1102 86-87 1.5700077649880662 1102 88-89 1.7300856960945445 1102 90-91 2.111727646308978 1102 92-93 2.0005772802602095 1102 94-95 1.8025871805966034 1102 96-97 1.8458401465207075 1102 98-99 1.3378359795215857 1102 100-101 1.2827863682126903 1103 1 0.24207820314812167 1103 2 0.32088073267058803 1103 3 0.2666759265905583 1103 4 0.19226432331740995 1103 5 0.14156066876061146 1103 6 0.24092625103028098 1103 7 0.3070147893022295 1103 8 0.33235146597501597 1103 9 0.42278912442292693 1103 10-11 0.374856320250224 1103 12-13 0.25305715995369127 1103 14-15 0.601150678136328 1103 16-17 0.328160193129996 1103 18-19 0.35238072997895387 1103 20-21 0.30682662193466825 1103 22-23 0.6226470728616178 1103 24-25 0.5790451272658288 1103 26-27 0.6739071241179673 1103 28-29 0.5629201557994747 1103 30-31 0.6214700772958892 1103 32-33 0.7092775352918963 1103 34-35 0.6760624548337262 1103 36-37 0.878416877996905 1103 38-39 0.9570680828579086 1103 40-41 1.2896863273663755 1103 42-43 1.3045665842771186 1103 44-45 1.3340269438655383 1103 46-47 1.11559309059475 1103 48-49 1.4298143196374156 1103 50-51 1.407466966182021 1103 52-53 1.4232671604102265 1103 54-55 1.3151427607302253 1103 56-57 1.2473195488512694 1103 58-59 1.4323490182793819 1103 60-61 1.2017473310469207 1103 62-63 1.293450020125949 1103 64-65 1.6723485142556278 1103 66-67 1.69424933046459 1103 68-69 1.4707872230783856 1103 70-71 1.4750492097564205 1103 72-73 1.252635866201718 1103 74-75 1.3609034379472043 1103 76-77 1.584051625299125 1103 78-79 1.5976627716844938 1103 80-81 1.880403060795949 1103 82-83 2.1222086068470354 1103 84-85 2.142454383508287 1103 86-87 2.0442420377401724 1103 88-89 1.6512707176501102 1103 90-91 2.297332330338861 1103 92-93 2.4116923501524923 1103 94-95 1.9007249699340214 1103 96-97 1.7722709490052395 1103 98-99 1.8875907330280768 1103 100-101 1.5781439055341 1104 1 0.1366113429642759 1104 2 0.3156728439998773 1104 3 0.2064579351849929 1104 4 0.26272734161616995 1104 5 0.11381372092487396 1104 6 0.13463409701338236 1104 7 0.11805095993884152 1104 8 0.2780812818491185 1104 9 0.2394885182588311 1104 10-11 0.35386824432318775 1104 12-13 0.23204062561861605 1104 14-15 0.3378392577771834 1104 16-17 0.5133817418365609 1104 18-19 0.44892587924329774 1104 20-21 0.33252456439176825 1104 22-23 0.4238508635215723 1104 24-25 0.28481364657486097 1104 26-27 0.434358474469434 1104 28-29 0.2882396014296802 1104 30-31 0.48861199937677213 1104 32-33 0.6726800225567544 1104 34-35 0.5632106377643709 1104 36-37 0.5138219834353066 1104 38-39 0.4137259274180778 1104 40-41 0.5950023579876245 1104 42-43 0.5807127466607938 1104 44-45 0.4559000762825107 1104 46-47 0.5358241728680113 1104 48-49 0.44040084742704977 1104 50-51 0.3355098814613342 1104 52-53 0.5940887546502367 1104 54-55 0.5587040459119308 1104 56-57 0.6450969471836068 1104 58-59 0.6214835980909896 1104 60-61 0.7123623172445939 1104 62-63 0.5618270371156164 1104 64-65 0.9189121614852027 1104 66-67 0.923382202771716 1104 68-69 1.5668343502020932 1104 70-71 1.2270626421250164 1104 72-73 1.0571266029899 1104 74-75 1.1119065968304938 1104 76-77 1.201784059346764 1104 78-79 1.4301992695928014 1104 80-81 1.5060896198898313 1104 82-83 1.6368219993738542 1104 84-85 1.8790433587210131 1104 86-87 1.937338027950478 1104 88-89 1.7618743204845693 1104 90-91 2.1316332495454375 1104 92-93 1.7270826572187126 1104 94-95 1.6727162047443471 1104 96-97 1.4327194535158974 1104 98-99 1.897721926289126 1104 100-101 2.5860411137642725 1105 1 0.20229290593707816 1105 2 0.11079029893425485 1105 3 0.13276402214865612 1105 4 0.15310410477454894 1105 5 0.04673004801269087 1105 6 -0.012275410441617396 1105 7 0.1401870528763567 1105 8 0.08443203925536125 1105 9 0.08828030185352986 1105 10-11 0.17818161076247208 1105 12-13 0.2559873495466647 1105 14-15 0.31997621291993283 1105 16-17 0.29286351543377265 1105 18-19 0.3616214407339342 1105 20-21 0.23001554716203287 1105 22-23 0.2649762055809717 1105 24-25 0.1613037732984992 1105 26-27 0.21595059801934013 1105 28-29 0.07008936011486355 1105 30-31 0.3774419252887071 1105 32-33 0.4419785364844486 1105 34-35 0.595156368103531 1105 36-37 0.4201936801115025 1105 38-39 0.44635321046568777 1105 40-41 0.5611998070918531 1105 42-43 0.3548139490265214 1105 44-45 0.8125769320253085 1105 46-47 0.8836665042709555 1105 48-49 1.095462300078637 1105 50-51 0.878881884015108 1105 52-53 0.5389035139615501 1105 54-55 0.7610574556256964 1105 56-57 0.7555351732579147 1105 58-59 0.8508999877913368 1105 60-61 0.4174368056181663 1105 62-63 0.5610551581445975 1105 64-65 0.6171218539037504 1105 66-67 0.8246155668296637 1105 68-69 1.016416899168167 1105 70-71 0.9278856375491671 1105 72-73 1.23576834234526 1105 74-75 1.024509779188957 1105 76-77 1.074541042695941 1105 78-79 1.0751503394457949 1105 80-81 0.9025069518573225 1105 82-83 1.2511401489993084 1105 84-85 1.6078243852789882 1105 86-87 1.631234641331492 1105 88-89 1.573371621987473 1105 90-91 1.9643254032725253 1105 92-93 2.0384126498489117 1105 94-95 1.6972479965094642 1105 96-97 1.7109695053508531 1105 98-99 2.1315868844208516 1105 100-101 1.8538707994904655 1106 1 -0.10104533997542475 1106 2 -0.10174370171855429 1106 3 -0.159870076426035 1106 4 -0.07613002742987618 1106 5 -0.06436281951582146 1106 6 -0.03805359265544439 1106 7 -0.14528193358273 1106 8 -0.16944477699769322 1106 9 -0.2353762396247916 1106 10-11 -0.11524377573222466 1106 12-13 -3.484607949388874E-5 1106 14-15 0.10906237428332588 1106 16-17 -0.09878345146071155 1106 18-19 -0.030781206124160576 1106 20-21 -0.18420587498017937 1106 22-23 -0.028319607516827716 1106 24-25 -0.08382337677915075 1106 26-27 -0.39825796569417804 1106 28-29 -0.14338726227265397 1106 30-31 -0.18003821833004707 1106 32-33 -0.29272438435709347 1106 34-35 -0.2889862219796555 1106 36-37 -0.3536275481651714 1106 38-39 -0.2782123230107345 1106 40-41 -0.40300529473701374 1106 42-43 -0.33874892374426935 1106 44-45 -0.33738399813364595 1106 46-47 -0.4710105018912003 1106 48-49 -0.4546776589722157 1106 50-51 0.07717764765740043 1106 52-53 -0.34121933348803424 1106 54-55 -0.22873493020628644 1106 56-57 0.18107794731554705 1106 58-59 0.42553426440580466 1106 60-61 0.4354920177327841 1106 62-63 0.19949691440813666 1106 64-65 0.03285859241276157 1106 66-67 0.04494177527468324 1106 68-69 0.08806406388464083 1106 70-71 0.1432653073842829 1106 72-73 0.32548654493475127 1106 74-75 0.42857106443835846 1106 76-77 0.387877211477651 1106 78-79 0.06302681940148247 1106 80-81 0.2820758977629012 1106 82-83 0.6133096626539718 1106 84-85 0.7788454434287573 1106 86-87 0.587859204886751 1106 88-89 0.6763221368191772 1106 90-91 0.4381248711314001 1106 92-93 0.42023478810669346 1106 94-95 0.43611507003000227 1106 96-97 0.5339465283278777 1106 98-99 0.6889344873130057 1106 100-101 0.9989583851721093 1107 1 -0.0389391665789276 1107 2 0.059112377685913486 1107 3 0.1412258706376548 1107 4 -0.06722334271674413 1107 5 0.012966302974597 1107 6 -0.06485635978079074 1107 7 -0.08328103125924713 1107 8 0.043385135025765464 1107 9 0.07276722980900274 1107 10-11 0.07425543473473084 1107 12-13 0.054783185351439556 1107 14-15 0.17922930412767357 1107 16-17 0.2208674621418112 1107 18-19 -0.02724146629410029 1107 20-21 -0.012798250346747864 1107 22-23 0.005291942223756507 1107 24-25 -0.03927397456845938 1107 26-27 0.04283440967238761 1107 28-29 0.11360010519858577 1107 30-31 -0.09317924397107191 1107 32-33 -0.14884232555567678 1107 34-35 0.09305754070926753 1107 36-37 0.14500204804507888 1107 38-39 0.3529591936446508 1107 40-41 0.32757313221237894 1107 42-43 0.19357305535190505 1107 44-45 0.3587435302519566 1107 46-47 0.6205649925068784 1107 48-49 0.659230341629339 1107 50-51 0.4879904907283148 1107 52-53 0.3698595070262911 1107 54-55 0.11556727296687797 1107 56-57 0.2608668412173394 1107 58-59 0.4702631913927995 1107 60-61 0.45652461416860746 1107 62-63 0.30525486613382924 1107 64-65 0.6492649011577818 1107 66-67 0.556593027248514 1107 68-69 0.6376241811866912 1107 70-71 0.8051318011056168 1107 72-73 1.1535262471679992 1107 74-75 0.9815731698598178 1107 76-77 1.237563278099202 1107 78-79 1.0151322408617425 1107 80-81 1.3197610620609694 1107 82-83 1.2604974211320545 1107 84-85 1.264471242361008 1107 86-87 2.044205546716963 1107 88-89 1.8222889388343653 1107 90-91 2.144830262514212 1107 92-93 2.069008192122034 1107 94-95 1.8847023698269787 1107 96-97 2.3734119016642197 1107 98-99 1.9438568498966617 1107 100-101 1.9252404364541622 1108 1 0.002341432826241885 1108 2 -0.021404094517244232 1108 3 0.04375103650358625 1108 4 -0.04613263120907618 1108 5 -0.08565243415650059 1108 6 -0.13136002027037819 1108 7 0.16629000511687053 1108 8 0.08967031004292636 1108 9 -0.019374156601429604 1108 10-11 0.08826109705456275 1108 12-13 -0.05950702283890763 1108 14-15 0.12181252307070878 1108 16-17 -0.050344789059288075 1108 18-19 -0.4254555477399933 1108 20-21 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-0.3528760947111511 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 23.0 5 70.0 6 146.0 7 285.0 8 619.0 9 1064.0 10 1691.0 11 2641.0 12 3871.0 13 5249.0 14 6382.0 15 8797.0 16 11143.0 17 14220.0 18 12252.0 19 8892.0 20 8367.0 21 9032.0 22 9690.0 23 10906.0 24 12181.0 25 13610.0 26 15437.0 27 17407.0 28 20516.0 29 23978.0 30 27653.0 31 32907.0 32 38809.0 33 46749.0 34 59037.0 35 72729.0 36 81655.0 37 80494.0 38 29737.0 39 748.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.24590611353712 23.121893291833132 16.33068730837127 19.30151328625848 2 14.689901944418235 24.437686439878355 40.49238272886289 20.38002888684052 3 16.935518986092443 31.86415241125261 31.308111434617654 19.892217168037295 4 10.925478414212005 20.43004666216736 39.50768873504162 29.136786188579016 5 10.305169566062984 41.810233470828486 34.45603299883599 13.428563964272547 6 25.24270858279088 41.59522202628466 18.47067104986248 14.691398341061982 7 24.214689511534917 36.911153201402044 23.100457913337543 15.7736993737255 8 25.116727987866383 35.51854512732313 21.230197606658972 18.134529278151508 9 27.273466165064324 17.369287306674096 22.144669314012354 33.21257721424922 10-11 22.401759083883046 30.24956639743394 27.761739925543726 19.586934593139283 12-13 24.835666441701317 28.768568712399944 28.388377274145675 18.00738757175306 14-15 22.114746841413943 29.311823743278254 28.399770680483893 20.17365873482391 16-17 17.92255386468697 32.62897408544329 29.630911690215463 19.81756035965428 18-19 17.79657326976248 29.71603567515004 34.55460489553625 17.93278615955123 20-21 18.925899317121313 29.010733024187402 34.706565359690565 17.35680229900072 22-23 18.970021553131737 28.997597950638248 34.54103440518436 17.491346091045653 24-25 18.261546678742384 29.8729582313719 34.659993091296485 17.20550199858923 26-27 17.610958904109587 29.915630953460948 34.86941848861472 17.603991653814752 28-29 17.476977336555418 29.18584314835376 35.60011320836871 17.73706630672211 30-31 17.70927220081423 29.938461091880203 34.836246997438295 17.51601970986727 32-33 17.18728229065069 30.410149900143978 34.60236113046305 17.800206678742278 34-35 17.739850651051967 29.961744663957607 34.898940158087214 17.399464526903206 36-37 18.003182936095264 29.672860063295868 34.02584811168288 18.29810888892598 38-39 17.43119132882278 30.177180241630534 34.45231041235123 17.939318017195454 40-41 17.37484776477317 29.904732320028565 34.990005820865406 17.730414094332858 42-43 17.438347425799556 29.738599326667746 34.11315436348766 18.709898884045046 44-45 17.285879537237676 30.081873110467015 33.79707776421531 18.835169588079996 46-47 18.031335495903452 30.102105240241222 32.911777298819295 18.95478196503603 48-49 17.712102410032003 30.46241264450395 32.28383369980914 19.541651245654904 50-51 18.010870906174937 30.41923381156612 32.05618328144617 19.513712000812777 52-53 17.8805361432231 30.695941189703845 32.09254058447449 19.330982082598567 54-55 17.893380938903768 30.63208005863613 31.863656485170427 19.610882517289678 56-57 18.068708771471492 30.813866573777748 30.938540918294038 20.178883736456722 58-59 17.668125312955826 30.090203847633145 31.95066727625019 20.291003563160835 60-61 18.158840049637515 31.110675694308377 31.001748924157653 19.72873533189646 62-63 18.228869585410635 30.855666586840254 31.632885579721187 19.282578248027924 64-65 18.115166376509535 30.31199813641473 31.546841085088488 20.025994401987244 66-67 17.771686753982628 30.63511622776462 31.018212112767635 20.574984905485113 68-69 18.048196740584206 30.48178598046266 31.25277127267001 20.217246006283123 70-71 18.3063870611449 30.067438854736867 31.04567598144833 20.580498102669907 72-73 17.746803494077238 30.067729132816005 30.73565899292375 21.449808380183004 74-75 17.58191278601441 30.423370271192095 30.553124478406957 21.441592464386535 76-77 17.19526266518625 29.784323543712222 32.00705375220429 21.013360038897233 78-79 17.14548001074028 29.325539372564386 31.712276576753094 21.816704039942234 80-81 17.11224319479822 30.431933468312543 30.746231830419667 21.709591506469568 82-83 17.586227916886795 29.449061522116494 30.897998223481395 22.066712337515312 84-85 17.04951368800266 29.105856499859932 30.93201211679616 22.912617695341243 86-87 16.946008573661718 29.565416806670243 30.915184322115387 22.573390297552653 88-89 17.18292585577544 28.938090987597874 30.832371787893965 23.04661136873272 90-91 17.028708109665427 28.750853608887837 31.10068194369556 23.119756337751173 92-93 17.161144714927957 28.962289139576992 30.703236972826264 23.173329172668787 94-95 16.734011132156258 28.00593618241061 31.098121176496203 24.16193150893693 96-97 16.970527340929426 28.05290898548537 30.779055565755602 24.1975081078296 98-99 16.655128121394206 28.0460670977293 30.769018643096103 24.52978613778039 100-101 16.606361439487948 27.674511426062598 31.216119130037228 24.503008004412223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3436.0 1 2224.5 2 1216.0 3 1417.5 4 1290.0 5 1326.0 6 2122.5 7 3969.0 8 5560.5 9 5639.5 10 4405.0 11 3159.0 12 2845.5 13 2727.5 14 2570.0 15 2420.0 16 2274.0 17 2187.0 18 2173.0 19 2151.5 20 2278.5 21 2524.5 22 2671.5 23 2993.0 24 3310.5 25 3777.0 26 4510.5 27 5273.5 28 6066.0 29 7492.5 30 9849.5 31 12059.0 32 15579.0 33 20101.0 34 23311.0 35 26734.0 36 32694.0 37 36613.5 38 36408.5 39 35362.0 40 33027.0 41 29770.5 42 26363.5 43 25132.5 44 25089.5 45 24433.5 46 22190.0 47 19586.0 48 19202.0 49 17565.0 50 14511.0 51 11553.0 52 9121.5 53 8007.0 54 7685.0 55 7310.0 56 6620.0 57 6121.5 58 5874.5 59 5535.5 60 4843.5 61 4258.0 62 3646.5 63 2421.5 64 1390.0 65 1030.5 66 808.0 67 606.0 68 450.5 69 339.5 70 285.5 71 257.5 72 219.0 73 205.0 74 167.0 75 123.5 76 98.0 77 55.5 78 31.0 79 23.5 80 13.5 81 6.5 82 3.5 83 1.5 84 2.0 85 1.0 86 1.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023657646281903352 2 0.014513893424480584 3 0.002467361882161699 4 0.0 5 1.4513893424480584E-4 6 0.0 7 0.0 8 0.0 9 4.354168027344175E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013062504082032526 26-27 0.008345488719076337 28-29 0.0 30-31 0.0 32-33 4.354168027344175E-4 34-35 0.010377433798503618 36-37 0.0012336809410808496 38-39 0.013352781950522138 40-41 0.01364305981901175 42-43 0.004862154297200996 44-45 2.902778684896117E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.256946712240292E-5 66-67 4.354168027344175E-4 68-69 9.434030725912379E-4 70-71 7.256946712240292E-5 72-73 7.256946712240292E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001015972539713641 84-85 0.004934723764323398 86-87 0.003555903888997743 88-89 0.0 90-91 0.0012336809410808496 92-93 3.628473356120146E-4 94-95 0.0 96-97 7.256946712240292E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 688995.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.69467694825642 #Duplication Level Percentage of deduplicated Percentage of total 1 74.63754767703324 24.40250509510689 2 11.809592389137796 7.72221616106896 3 4.410353624342592 4.325852609263587 4 2.254331324442596 2.948185375479429 5 1.313159479496957 2.1466662481846783 6 0.8428384872650055 1.6533799236411895 7 0.6062485958123895 1.3874771393294112 8 0.43769966034128527 1.144835919617591 9 0.3367645308810793 0.9909366790309212 >10 2.6044639567604886 17.544677897545498 >50 0.43689950539323974 9.993910589892268 >100 0.2927493630752104 17.220567630702146 >500 0.012457419705328102 2.5678262664908433 >1k 0.0031143549263320256 1.4773037762692465 >5k 0.0013347235398565825 2.9756994240980674 >10k+ 4.4490784661886077E-4 1.4979592642793127 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10298 1.4946407448530106 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8095 1.1748996727117034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6656 0.9660447463334276 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5706 0.8281627588008621 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2117 0.307259123796254 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1638 0.23773757429299194 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1606 0.2330931283971582 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1400 0.2031945079427282 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1251 0.1815688067402521 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1108 0.16081393914324488 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.15036393587761884 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 896 0.13004448508334604 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 854 0.12394864984506418 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 802 0.11640142526433428 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 777 0.11277295190821415 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 772 0.1120472572369901 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 724 0.10508058839323942 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 694 0.10072642036589524 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.1770840136720878E-4 0.0 20-21 0.0 0.0 0.0 0.001015972539713641 0.0 22-23 0.0 0.0 0.0 0.002830209217773714 0.0 24-25 0.0 0.0 0.0 0.008853474988933158 0.0 26-27 0.0 0.0 0.0 0.01886806145182476 0.0 28-29 0.0 0.0 0.0 0.03759098396940472 0.0 30-31 0.0 0.0 0.0 0.09005870869890202 0.0 32-33 0.0 0.0 0.0 0.16516810717058905 0.0 34-35 0.0 0.0 0.0 0.26698306954332035 0.0 36-37 0.0 0.0 0.0 0.41154144805114695 0.0 38-39 0.0 0.0 0.0 0.6248956813910116 0.0 40-41 0.0 0.0 0.0 0.8600933243347194 0.0 42-43 0.0 0.0 0.0 1.0813576295909257 0.0 44-45 0.0 0.0 0.0 1.3328834026371745 0.0 46-47 0.0 0.0 0.0 1.6387637065581027 0.0 48-49 0.0 0.0 0.0 1.968591934629424 0.0 50-51 0.0 0.0 0.0 2.2962430786870733 0.0 52-53 0.0 0.0 0.0 2.6218622776652953 0.0 54-55 0.0 0.0 0.0 2.9516905057366163 0.0 56-57 0.0 0.0 0.0 3.328906595838867 0.0 58-59 0.0 0.0 0.0 3.7546716594460046 0.0 60-61 0.0 0.0 0.0 4.20663430068433 0.0 62-63 0.0 0.0 0.0 4.6381323521941376 0.0 64-65 0.0 0.0 0.0 5.085015130733895 0.0 66-67 0.0 0.0 0.0 5.58015660491005 0.0 68-69 0.0 0.0 0.0 6.107664061422797 0.0 70-71 0.0 0.0 0.0 6.681615976893882 0.0 72-73 0.0 0.0 0.0 7.265437339893613 0.0 74-75 0.0 0.0 0.0 7.842582311918084 0.0 76-77 0.0 0.0 0.0 8.427201939056161 0.0 78-79 0.0 0.0 0.0 9.090704576956291 0.0 80-81 0.0 0.0 0.0 9.787371461331361 0.0 82-83 0.0 0.0 0.0 10.50101960101307 0.0 84-85 0.0 0.0 0.0 11.225698299697385 0.0 86-87 0.0 0.0 0.0 11.948417622769396 0.0 88-89 0.0 0.0 0.0 12.685287991930275 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 120 0.0 47.519676 1 GGTATCA 4685 0.0 40.67319 1 GTGGTAC 205 0.0 34.770493 1 GTATCAA 7510 0.0 34.23188 1 GCCGGTG 30 0.003951637 31.665987 92-93 CATTCCG 60 0.0039502773 31.665987 9 GGTACTC 230 0.0 30.977598 3 GTCACTA 190 1.2732926E-11 30.001535 2 ACGGAGG 165 2.6448106E-9 28.787262 8 CTACACG 165 2.6448106E-9 28.787262 4 TGGTACT 250 0.0 28.501457 2 GTACATG 5990 0.0 28.32141 1 CATGGGG 1710 0.0 28.054955 4 ACATGGG 6060 0.0 26.963118 3 ATGGGGA 805 0.0 26.552225 5 GGCATTC 90 8.94501E-4 26.388327 7 TACACGG 180 6.690243E-9 26.388327 5 ATCAACG 9685 0.0 26.3856 3 TCAACGC 9720 0.0 26.192854 4 TACATGG 6425 0.0 26.024677 2 >>END_MODULE