FastQCFastQC Report
Thu 26 May 2016
SRR938603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491665
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62030.24894999929765838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46510.18666233221560682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.1332041024776605No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30960.12425426371522658No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29840.11975927743095481No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28520.11446161502449165No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26470.10623418477202994No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.049.9842571
GTATCAA66400.043.9803851
ATCAACG83850.034.597973
TATCAAC85450.034.117112
TCAACGC85000.034.0179754
CAACGCA88200.032.6759225
AACGCAG91350.031.5231866
GTGGTAT18450.029.9033851
TGGTATC18850.027.5003972
ACGCAGA105400.027.138037
CGCAGAG106700.026.6737968
TAAGGTG12400.025.3131435
GTACATG86650.025.2492241
TACATGG86450.024.755482
GCAGAGT117800.023.5150389
ACATGGG88850.023.390953
GAGTACT66350.022.1988312-13
CATGGGG63450.021.2118554
AGGTAAG13150.020.9761642
GTACTTT70750.020.84912314-15
Produced by FastQC (version 0.11.5)