##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938603_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2491665 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16127248245651 31.0 31.0 34.0 28.0 34.0 2 31.190550094013442 31.0 31.0 34.0 28.0 34.0 3 31.239417016332453 31.0 31.0 34.0 27.0 34.0 4 34.89793531634469 37.0 35.0 37.0 32.0 37.0 5 34.86642787052031 37.0 35.0 37.0 32.0 37.0 6 34.88951283579454 37.0 35.0 37.0 32.0 37.0 7 34.80205926559148 37.0 35.0 37.0 32.0 37.0 8 34.768919979210686 37.0 35.0 37.0 32.0 37.0 9 36.26535910726362 39.0 35.0 39.0 32.0 39.0 10-11 36.09452454483247 39.0 35.0 39.0 30.5 39.0 12-13 35.9522439814341 38.0 35.0 39.0 30.0 39.0 14-15 36.925579883331025 39.0 36.0 41.0 31.0 41.0 16-17 36.75385194237588 39.0 36.0 41.0 30.0 41.0 18-19 36.88958367196232 39.0 36.0 41.0 30.5 41.0 20-21 36.85839167785396 39.0 36.0 41.0 30.0 41.0 22-23 36.774010551177625 39.0 36.0 41.0 30.0 41.0 24-25 36.631961961178575 39.0 36.0 41.0 30.0 41.0 26-27 36.419033457547464 39.0 35.5 41.0 29.5 41.0 28-29 36.20700134247582 39.0 35.0 40.0 29.0 41.0 30-31 35.955548599029164 38.5 35.0 40.0 27.5 41.0 32-33 35.6886343067788 38.0 35.0 40.0 27.0 41.0 34-35 35.43932571192356 38.0 34.0 40.0 26.5 41.0 36-37 35.15724726237275 38.0 34.0 40.0 25.5 41.0 38-39 35.02621720817204 38.0 34.0 40.0 25.0 41.0 40-41 34.92798309564087 38.0 34.0 40.0 25.0 41.0 42-43 34.633148316487166 38.0 33.0 40.0 24.0 41.0 44-45 34.29570949545786 38.0 33.0 40.0 23.5 41.0 46-47 33.91138997417389 38.0 33.0 40.0 22.5 41.0 48-49 33.55097454914686 38.0 32.5 40.0 20.5 41.0 50-51 32.83605039200695 36.5 31.5 39.5 19.0 40.5 52-53 32.8475346806252 37.0 31.5 39.0 19.0 40.0 54-55 32.938268386801596 37.0 32.0 40.0 18.0 41.0 56-57 32.597517322754065 36.5 31.0 39.5 15.0 41.0 58-59 32.20949124380685 36.0 31.0 39.0 12.5 41.0 60-61 31.717517403021674 35.5 30.5 39.0 8.0 40.0 62-63 31.13842069459578 35.0 29.5 39.0 7.0 40.0 64-65 30.604265822251385 35.0 29.0 38.0 2.0 40.0 66-67 30.058054754551677 34.5 28.0 38.0 2.0 40.0 68-69 29.46171676369014 34.0 27.5 37.0 2.0 39.5 70-71 28.81405726692794 34.0 26.5 36.5 2.0 39.0 72-73 28.175296438325375 33.5 26.0 36.0 2.0 39.0 74-75 27.504576859248736 33.0 25.5 35.5 2.0 38.0 76-77 26.758874086203402 32.5 23.5 35.0 2.0 37.0 78-79 25.97889403270504 32.0 21.0 35.0 2.0 36.5 80-81 25.28401189566013 31.0 19.0 35.0 2.0 36.0 82-83 24.551191071030818 31.0 16.5 34.0 2.0 36.0 84-85 23.850821037338484 30.5 9.0 34.0 2.0 35.0 86-87 23.081256709870708 30.0 4.5 34.0 2.0 35.0 88-89 22.25197167355965 29.0 2.0 34.0 2.0 35.0 90-91 21.331351525987643 29.0 2.0 34.0 2.0 35.0 92-93 20.37353255754686 27.0 2.0 33.5 2.0 35.0 94-95 19.349282106543214 25.5 2.0 33.0 2.0 35.0 96-97 18.084604069969277 24.5 2.0 32.5 2.0 34.0 98-99 16.551366656432545 10.5 2.0 32.0 2.0 34.0 100-101 13.045868726333596 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.476925991678236 1101 2 0.37082727120073145 1101 3 0.4335339208739235 1101 4 0.29912059485241826 1101 5 0.2564934896750941 1101 6 0.3065390256384575 1101 7 0.20406390329647195 1101 8 0.44776622629276375 1101 9 0.4522986248835892 1101 10-11 0.5197559857977581 1101 12-13 0.5831930381597275 1101 14-15 0.7660229084273098 1101 16-17 0.6409374158893684 1101 18-19 0.6002813984256008 1101 20-21 0.640650959231948 1101 22-23 0.6939990252587904 1101 24-25 0.8086978930976443 1101 26-27 0.7688444469972708 1101 28-29 0.7689826108377176 1101 30-31 0.629295599056924 1101 32-33 0.8581614387270164 1101 34-35 0.6592706655617562 1101 36-37 0.7147771214959846 1101 38-39 0.8437267681410532 1101 40-41 0.9794983864809197 1101 42-43 1.1043012195587423 1101 44-45 1.0013525878657745 1101 46-47 0.9038570517114266 1101 48-49 0.9238122940625146 1101 50-51 1.0074616570389097 1101 52-53 0.9039176071113459 1101 54-55 0.8823845916754749 1101 56-57 0.8478308566053201 1101 58-59 0.9176992610470123 1101 60-61 0.9893947822288673 1101 62-63 0.8528957346103141 1101 64-65 0.7707974690686719 1101 66-67 0.9493019067906481 1101 68-69 0.7938093656838845 1101 70-71 0.9744505009734326 1101 72-73 0.9996064840727321 1101 74-75 0.6433393945153654 1101 76-77 0.9430429147053765 1101 78-79 1.0470372462854876 1101 80-81 1.1269632773496774 1101 82-83 1.103716214813307 1101 84-85 0.9254364700167876 1101 86-87 0.9348144195363801 1101 88-89 0.772163375117124 1101 90-91 0.5968078456920054 1101 92-93 0.8839990284124966 1101 94-95 0.8193667846680697 1101 96-97 0.8731461057711627 1101 98-99 1.0701353885149985 1101 100-101 1.0471643757976086 1102 1 0.36729920605570854 1102 2 0.2504156145541039 1102 3 0.25978851339229436 1102 4 0.15441050158896985 1102 5 0.14226535391639317 1102 6 0.17305266188530766 1102 7 0.11832736611955141 1102 8 0.3006457031952934 1102 9 0.33462064071011355 1102 10-11 0.5453567867586528 1102 12-13 0.4183184171227836 1102 14-15 0.4710687372644742 1102 16-17 0.6399827822631394 1102 18-19 0.5461999181573773 1102 20-21 0.547703927347662 1102 22-23 0.6605565794697768 1102 24-25 0.49602543279242184 1102 26-27 0.36051978485247105 1102 28-29 0.2235208539906921 1102 30-31 0.38086074858674834 1102 32-33 0.5045728512957481 1102 34-35 0.4140449577358467 1102 36-37 0.5726047157871221 1102 38-39 0.554113968824467 1102 40-41 0.6307734526388771 1102 42-43 0.6502873424455444 1102 44-45 0.5220687798651511 1102 46-47 0.4898677750812439 1102 48-49 0.7221037487667985 1102 50-51 0.6491516877413446 1102 52-53 0.484550908971471 1102 54-55 0.2819030195568857 1102 56-57 0.4157705822103779 1102 58-59 0.5959390740062673 1102 60-61 0.5694459101256903 1102 62-63 0.8038289311315623 1102 64-65 0.918695749604101 1102 66-67 0.5767086433406696 1102 68-69 0.6028072492715708 1102 70-71 0.8857960285670785 1102 72-73 0.9857143568696074 1102 74-75 1.005258023439886 1102 76-77 1.0056598954617577 1102 78-79 1.0476259236401972 1102 80-81 1.145030260675302 1102 82-83 1.0689995860429455 1102 84-85 1.2490487586967127 1102 86-87 1.4418267615280556 1102 88-89 1.3482865348982251 1102 90-91 1.4371952711840166 1102 92-93 1.8991418139836398 1102 94-95 1.8313416960292521 1102 96-97 1.703508432989704 1102 98-99 1.4390888402903386 1102 100-101 1.3566189419812584 1103 1 0.4792565729040028 1103 2 0.34806516606445825 1103 3 0.39222280636155915 1103 4 0.3306660760007034 1103 5 0.34963569018216134 1103 6 0.4854773534218424 1103 7 0.4462686134686962 1103 8 0.40272846576974075 1103 9 0.5879630681349539 1103 10-11 0.7161498023188031 1103 12-13 0.8003019691201843 1103 14-15 0.9860151683726102 1103 16-17 0.9742383668217016 1103 18-19 0.9967610217006424 1103 20-21 0.7813849004027702 1103 22-23 0.8603563540834358 1103 24-25 0.895451059105163 1103 26-27 0.8138751494786121 1103 28-29 0.8302721071235197 1103 30-31 0.8346820744215862 1103 32-33 0.8718821924624294 1103 34-35 1.0567112134561185 1103 36-37 0.986999365747053 1103 38-39 1.0433890130899854 1103 40-41 0.9548217280592084 1103 42-43 0.9440335532407502 1103 44-45 0.9278043834236414 1103 46-47 0.7429344149856618 1103 48-49 0.769928269583005 1103 50-51 1.1022642420268554 1103 52-53 0.9086042260952567 1103 54-55 0.8935978373199163 1103 56-57 1.035595701689239 1103 58-59 1.006488473821591 1103 60-61 1.1784900285188336 1103 62-63 1.033717369966503 1103 64-65 1.0137462078600663 1103 66-67 1.1082773170492324 1103 68-69 1.4021508607039976 1103 70-71 1.1829942207834492 1103 72-73 1.2197916902587487 1103 74-75 1.5185529795802353 1103 76-77 1.390745125617105 1103 78-79 1.2498543565646116 1103 80-81 1.2664749877510886 1103 82-83 1.302217768772035 1103 84-85 1.5977992406708772 1103 86-87 1.691478360794438 1103 88-89 1.6022421317598123 1103 90-91 1.581740354159674 1103 92-93 1.5777008761126439 1103 94-95 1.508508106488602 1103 96-97 1.8605619346588576 1103 98-99 1.7221140589544106 1103 100-101 1.3513808816228714 1104 1 0.42134121154665394 1104 2 0.37793780007036304 1104 3 0.38295254046833804 1104 4 0.38450005957276545 1104 5 0.35682489480949897 1104 6 0.507008055419746 1104 7 0.41437290061485044 1104 8 0.63602879885336 1104 9 0.6864644302603793 1104 10-11 0.7034593427643117 1104 12-13 0.7644560694691407 1104 14-15 0.8334392802303867 1104 16-17 0.890119028239603 1104 18-19 0.8015968937769671 1104 20-21 0.944315264134012 1104 22-23 0.9587316775045949 1104 24-25 0.8854270217786322 1104 26-27 0.909764842004158 1104 28-29 0.863479883657746 1104 30-31 0.8478015577290279 1104 32-33 0.8859675367372617 1104 34-35 0.8411584987485341 1104 36-37 1.0222931251317036 1104 38-39 1.066370890431486 1104 40-41 0.97073044250903 1104 42-43 0.9869076294247279 1104 44-45 0.8516028746811415 1104 46-47 0.8724756725520209 1104 48-49 0.729201619156548 1104 50-51 0.7541964791900213 1104 52-53 0.8247634184386214 1104 54-55 0.9262803116034775 1104 56-57 1.0070027565653703 1104 58-59 0.9601216106042685 1104 60-61 0.9143827293629379 1104 62-63 1.143327978811115 1104 64-65 1.0063281495512868 1104 66-67 1.110810933394184 1104 68-69 1.1308400535656702 1104 70-71 1.1996887738658941 1104 72-73 1.1866914195869747 1104 74-75 1.0585022503475159 1104 76-77 1.0164104815530202 1104 78-79 1.2532589395749056 1104 80-81 1.4296934948776219 1104 82-83 1.3796932758144358 1104 84-85 1.6230382028255868 1104 86-87 1.7201695838097386 1104 88-89 1.458236337209204 1104 90-91 1.4778210964453606 1104 92-93 1.5206560969218437 1104 94-95 1.652196766434944 1104 96-97 1.601704505119411 1104 98-99 1.4921367951370215 1104 100-101 1.4729671667662547 1105 1 0.12906381669922595 1105 2 0.09744589159057782 1105 3 0.10506547043893022 1105 4 0.14657073722637648 1105 5 0.14421036452277036 1105 6 0.17184921139311626 1105 7 0.13666215355222278 1105 8 0.12933630507022542 1105 9 0.2964832197691294 1105 10-11 0.33624424252470675 1105 12-13 0.4047855178197679 1105 14-15 0.41306132380171334 1105 16-17 0.2431127921852294 1105 18-19 0.3416523599445256 1105 20-21 0.31603741647735717 1105 22-23 0.4505906683237981 1105 24-25 0.5114813479218867 1105 26-27 0.4730649352478906 1105 28-29 0.40070744059635643 1105 30-31 0.5955733318003382 1105 32-33 0.5930792346696521 1105 34-35 0.6100251673216661 1105 36-37 0.782113166059375 1105 38-39 0.5614873369339932 1105 40-41 0.8317928343167367 1105 42-43 0.7003799190851296 1105 44-45 0.6542594691082328 1105 46-47 0.6074003181910115 1105 48-49 0.5883339412455655 1105 50-51 0.7157211038776907 1105 52-53 0.6777919283806426 1105 54-55 0.5942959477463745 1105 56-57 0.5058282085337353 1105 58-59 0.6222289456545056 1105 60-61 0.5850389871465147 1105 62-63 0.6379682051327507 1105 64-65 0.457311327895745 1105 66-67 0.5415334915766898 1105 68-69 0.5906510857232732 1105 70-71 0.5773707906568788 1105 72-73 0.5297750178616205 1105 74-75 0.6258628096261845 1105 76-77 0.8413936382645772 1105 78-79 0.8539664688174753 1105 80-81 0.9615741689766217 1105 82-83 0.9412891112733988 1105 84-85 0.9825248748095987 1105 86-87 1.0971701641938232 1105 88-89 1.0533056062053028 1105 90-91 0.7629145157610822 1105 92-93 1.057822237163208 1105 94-95 1.0491954087481012 1105 96-97 1.387556779959798 1105 98-99 1.2368149160794673 1105 100-101 1.0082853978646167 1106 1 0.18128701368359046 1106 2 0.21722482084195605 1106 3 0.08776453130235495 1106 4 0.2384062012410766 1106 5 0.2826105091798894 1106 6 0.0043586308326126755 1106 7 0.30578307414686634 1106 8 0.20517636934656025 1106 9 0.17837869379605564 1106 10-11 0.1833675537345627 1106 12-13 0.1276503331129888 1106 14-15 0.37196947492714827 1106 16-17 0.19419817009976015 1106 18-19 -0.04867046742437964 1106 20-21 0.10330545260214308 1106 22-23 -0.008535072701334911 1106 24-25 -0.015655568367229478 1106 26-27 -0.008661860554049383 1106 28-29 0.22106063623524363 1106 30-31 -0.015888623272545033 1106 32-33 0.13483151641622015 1106 34-35 -0.13051276678418589 1106 36-37 -0.009936395216605831 1106 38-39 0.1132024802920597 1106 40-41 0.36994909849637025 1106 42-43 -0.12651232093546128 1106 44-45 0.14866631078900383 1106 46-47 0.12489084717247323 1106 48-49 0.36322602682375305 1106 50-51 0.1862653409432511 1106 52-53 0.12598423747295584 1106 54-55 0.24234424813568012 1106 56-57 -0.4132301908858693 1106 58-59 -0.1222834488645006 1106 60-61 0.5016872450052183 1106 62-63 0.4675813873227739 1106 64-65 0.46240085364997086 1106 66-67 0.07750589884897963 1106 68-69 0.3219508438470129 1106 70-71 0.24371845319019414 1106 72-73 0.5098202630028368 1106 74-75 0.7854339431022979 1106 76-77 0.6764648416671442 1106 78-79 0.8922939754376458 1106 80-81 0.8669159310141765 1106 82-83 0.694922671019107 1106 84-85 0.8578821886670731 1106 86-87 0.7238589175002268 1106 88-89 0.8111326162666046 1106 90-91 0.9152636982552664 1106 92-93 0.6890891468015745 1106 94-95 0.725560385631109 1106 96-97 0.5512527636171995 1106 98-99 0.9244141044315626 1106 100-101 0.15565720219256995 1107 1 0.2559457133645999 1107 2 0.07716405692170625 1107 3 0.22144544485646733 1107 4 0.16852220180464172 1107 5 0.1606367294256188 1107 6 0.21093023796966293 1107 7 0.28118112840088116 1107 8 0.2629779581677312 1107 9 0.4570220905064275 1107 10-11 0.49767294120389494 1107 12-13 0.5135221075133884 1107 14-15 0.5594771065431701 1107 16-17 0.6731251558552813 1107 18-19 0.5407571162163194 1107 20-21 0.5521490956917816 1107 22-23 0.48408424242208525 1107 24-25 0.6660874385436699 1107 26-27 0.5149737148788276 1107 28-29 0.6648487356106259 1107 30-31 0.5983387320518787 1107 32-33 0.7311406682140316 1107 34-35 0.6102964554593129 1107 36-37 0.7102094815431812 1107 38-39 0.5363478982517336 1107 40-41 0.527670993376951 1107 42-43 0.7548228235143242 1107 44-45 0.6489678183266889 1107 46-47 0.6447534238950468 1107 48-49 0.6635823013668443 1107 50-51 0.7768063276886252 1107 52-53 0.7173493303709435 1107 54-55 0.9973899294538455 1107 56-57 0.8268812939285723 1107 58-59 0.822963996124642 1107 60-61 0.6525240313813789 1107 62-63 0.9096308871584 1107 64-65 0.931073909800606 1107 66-67 1.0325911020035043 1107 68-69 1.0945828753199756 1107 70-71 1.37531728126298 1107 72-73 1.404670069186885 1107 74-75 1.4666297134710362 1107 76-77 1.4447465088094695 1107 78-79 1.434319848873841 1107 80-81 1.4851443123168089 1107 82-83 1.2747491217486697 1107 84-85 1.4267557440789282 1107 86-87 1.274442460665366 1107 88-89 1.419682338277088 1107 90-91 1.2942891634677451 1107 92-93 1.5345974214602762 1107 94-95 1.5928576917158104 1107 96-97 1.522748488105929 1107 98-99 1.476081860899015 1107 100-101 1.3655746542985359 1108 1 0.20808072833601798 1108 2 0.22139053364795558 1108 3 0.1972298564343653 1108 4 0.14428120733953165 1108 5 0.23471712140457157 1108 6 0.1467110201128392 1108 7 0.13304575048599787 1108 8 0.27144542935909755 1108 9 0.13853556478280638 1108 10-11 0.381491106524642 1108 12-13 0.3559447551860089 1108 14-15 0.4879845015164008 1108 16-17 0.5006961566718999 1108 18-19 0.5367901440722349 1108 20-21 0.4180632595607534 1108 22-23 0.34534365626838337 1108 24-25 0.5034796897005549 1108 26-27 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-1.64332735329263 2316 68-69 -1.7911621404389564 2316 70-71 -2.287769131726904 2316 72-73 -1.933642428758791 2316 74-75 -1.848130140882116 2316 76-77 -2.1465674901606313 2316 78-79 -2.380067999767384 2316 80-81 -2.4990264903843062 2316 82-83 -2.621869227270551 2316 84-85 -2.470764607401822 2316 86-87 -2.783755506560439 2316 88-89 -2.9417021832759396 2316 90-91 -2.9552246287919353 2316 92-93 -2.8868219413895524 2316 94-95 -3.0859008040458953 2316 96-97 -3.2015316592939413 2316 98-99 -3.208059140441314 2316 100-101 -2.9692442914783275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8325.0 3 3093.0 4 3271.0 5 3690.0 6 4374.0 7 5577.0 8 6930.0 9 8784.0 10 11067.0 11 13747.0 12 16486.0 13 19208.0 14 21550.0 15 24832.0 16 28252.0 17 32495.0 18 31624.0 19 31648.0 20 33380.0 21 36210.0 22 40076.0 23 44665.0 24 50131.0 25 55429.0 26 62531.0 27 70820.0 28 80612.0 29 91494.0 30 105563.0 31 122526.0 32 144480.0 33 170737.0 34 207057.0 35 248476.0 36 283297.0 37 258473.0 38 106070.0 39 4685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90343068236367 17.766131142645683 14.238600113344646 24.091838061646 2 15.861164837125136 19.195481175717145 39.4421217485494 25.501232238608324 3 19.438712041581883 22.950840091675587 28.92353510319932 28.686912763543205 4 13.44695333516829 15.656375504529462 35.37948451102079 35.51718664928145 5 14.675052326630727 36.52453090939202 33.30562410285002 15.494792661127232 6 34.09505598026731 34.56929139868315 16.628891201034843 14.706761420014702 7 30.275789918240157 31.664053210135556 19.54468047181923 18.515476399805056 8 27.467205888728845 34.13971760693313 19.02343480278571 19.36964170155231 9 26.912735351050515 16.632879257313938 19.29238326082087 37.16200213081468 10-11 25.823135326836805 25.697169400680064 26.640367883146027 21.839327389337107 12-13 26.578262927972624 22.862912633210954 27.020997728401646 23.537826710414777 14-15 23.16081205830996 24.548807985131653 24.954941518818575 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23.428720553252568 25.662307159561976 27.62430617591264 23.284666111272823 42-43 23.23493828253393 25.688902860155054 27.40026252330301 23.67589633400801 44-45 23.168306380273346 25.92326204539288 27.52882610098688 23.379605473346892 46-47 23.513111965126008 26.229569720589417 26.917885925793023 23.339432388491552 48-49 23.411936445348154 26.10186308280038 26.90623015723298 23.579970314618485 50-51 23.524221100525637 26.389865556957677 26.814114111221354 23.271799231295333 52-53 23.7132986731711 26.044069487832083 26.865405355721556 23.37722648327526 54-55 23.188014305992585 26.29969412822933 26.997995306661487 23.5142962591166 56-57 23.301534381542062 26.407185400405687 26.921208134050513 23.370072084001738 58-59 23.330359499392355 25.833598436077793 26.94516337186606 23.890878692663794 60-61 22.945018233319043 26.164411387354058 27.345622959690797 23.544947419636102 62-63 23.064958876650824 26.288057310587952 27.183956695955334 23.463027116805886 64-65 22.822997474359784 26.25190777650342 27.085539631077914 23.83955511805888 66-67 22.79177093215099 26.21710406827547 27.131395883459202 23.85972911611434 68-69 23.23471004552693 25.756562872220545 27.356236948762387 23.65249013349014 70-71 23.012606641977833 26.080892636085512 26.981000008230062 23.925500713706597 72-73 23.07297958174308 26.14677812583678 26.803092144906554 23.977150147513587 74-75 22.928557291585122 26.50185693473088 26.80369162879933 23.765894144884665 76-77 23.10953777070725 26.07512653594882 26.885093094771147 23.930242598572793 78-79 23.21442117164394 26.208369940266312 26.909176142326952 23.668032745762794 80-81 23.253431488824056 26.39480052031721 26.640229382671155 23.71153860818757 82-83 23.097676503812625 26.387548511945795 26.66476823135063 23.850006752890952 84-85 23.09327216665111 26.276729067519145 26.755707535763346 23.874291230066394 86-87 23.20852292249349 26.230436325336605 26.607681291122248 23.953359461047654 88-89 23.086867843319897 26.338008094175088 26.479636083318614 24.095487979186405 90-91 23.075377356521013 26.344315041104117 26.493768137121858 24.08653946525301 92-93 23.27136588335683 25.809098662754838 26.687173273804753 24.23236218008358 94-95 22.941946467720822 25.764437091942856 26.902640476056632 24.39097596427969 96-97 23.20308668358783 25.485717222506736 26.865421743902345 24.445774350003084 98-99 23.07181856008806 25.687608603189112 26.42987884616118 24.810693990561646 100-101 23.58168059791472 25.03035902877616 26.78735706856793 24.60060330474119 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 439.0 1 305.5 2 236.5 3 312.5 4 283.0 5 285.0 6 456.0 7 992.0 8 1652.5 9 1869.5 10 1662.0 11 1450.0 12 1478.0 13 1591.5 14 1717.0 15 1916.5 16 2089.0 17 2246.0 18 2493.5 19 2767.5 20 3066.0 21 3610.0 22 4309.5 23 5153.5 24 6344.0 25 7990.0 26 10021.5 27 12691.5 28 15431.5 29 18242.5 30 21634.0 31 26516.5 32 33763.5 33 37459.0 34 38088.0 35 40980.5 36 45410.5 37 49393.0 38 52918.0 39 56178.5 40 59949.5 41 66381.0 42 71437.0 43 75613.0 44 80893.5 45 87709.5 46 92276.0 47 94305.0 48 95555.0 49 94932.0 50 95082.0 51 93913.0 52 93589.0 53 95735.0 54 97136.0 55 93712.5 56 86727.0 57 80724.5 58 75931.0 59 67979.0 60 55986.5 61 46532.5 62 39700.0 63 31468.5 64 24179.0 65 19104.0 66 14578.5 67 11155.5 68 8534.0 69 6326.0 70 4819.0 71 3581.0 72 2741.5 73 2254.5 74 1765.0 75 1374.0 76 983.0 77 621.5 78 400.0 79 262.0 80 160.5 81 110.5 82 74.0 83 41.0 84 26.0 85 18.5 86 17.0 87 13.0 88 8.5 89 8.0 90 5.0 91 2.0 92 2.0 93 3.0 94 2.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005979937110325827 2 0.10832114268972755 3 0.11594656585054572 4 0.11879606608432515 5 0.15756532278616908 6 0.09612046563241848 7 0.028535136143903777 8 0.026849516287301867 9 0.02404014985963201 10-11 0.052535152197426216 12-13 0.054100370635699425 14-15 0.029518414393588224 16-17 0.033852865453421706 18-19 0.03794651367659778 20-21 0.059377966139107785 22-23 0.03282945339762769 24-25 0.050628796407221684 26-27 0.05915723020550515 28-29 0.05458197630901425 30-31 0.06856860773819916 32-33 0.05183281059050875 34-35 0.06359201578061256 36-37 0.0545217755998499 38-39 0.032769252688463334 40-41 0.03772577774299515 42-43 0.03365219642287386 44-45 0.00694314845695549 46-47 0.011859539705377729 48-49 0.008147162640242568 50-51 0.00806689502802343 52-53 0.009471578241858356 54-55 0.010494990297652372 56-57 0.013464891949760503 58-59 0.019765899508962882 60-61 0.024923093594042538 62-63 0.01035452197626888 64-65 0.008729102828831324 66-67 0.009170574696036587 68-69 0.011337800225953328 70-71 0.03192644276016238 72-73 0.02841473472557507 74-75 0.02937794607220473 76-77 0.023056871609947564 78-79 0.0042541834476143466 80-81 0.004334451059833485 82-83 0.005598665952284918 84-85 0.004515053187326546 86-87 0.012561881312295192 88-89 0.039531799017925764 90-91 0.03680270020247505 92-93 0.030521759546327454 94-95 0.04836123636203101 96-97 0.043966584593033174 98-99 0.026428111323151385 100-101 0.07539135477682594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2491665.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.95148930060481 #Duplication Level Percentage of deduplicated Percentage of total 1 76.08543377517631 39.52751598702949 2 12.862393829834298 13.36441030861604 3 4.484170267273109 6.988779709869874 4 2.096658048264094 4.356980326456763 5 1.1556067591455959 3.001774609172952 6 0.7464858876500895 2.3268632163183667 7 0.48003720021406865 1.7457053229569437 8 0.3282531778579417 1.3642593165901111 9 0.2583999733710025 1.2081837106674185 >10 1.335691867753002 12.502727987416476 >50 0.10001221045875283 3.598226244488736 >100 0.060489274904772294 5.8805408893626305 >500 0.003339174489909778 1.2368773759095957 >1k 0.0029508983864292757 2.64690709525349 >5k 7.765522069550725E-5 0.2502478998911355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6203 0.24894999929765838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4651 0.18666233221560682 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3319 0.1332041024776605 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3096 0.12425426371522658 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2984 0.11975927743095481 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2852 0.11446161502449165 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2647 0.10623418477202994 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.0133806109569304E-5 0.0 0.0 0.0 4 0.0 4.0133806109569304E-5 0.0 0.0 0.0 5 0.0 4.0133806109569304E-5 0.0 0.0 4.0133806109569304E-5 6 4.0133806109569304E-5 4.0133806109569304E-5 0.0 0.0 4.0133806109569304E-5 7 4.0133806109569304E-5 4.0133806109569304E-5 0.0 0.0 4.0133806109569304E-5 8 4.0133806109569304E-5 4.0133806109569304E-5 0.0 0.0 4.0133806109569304E-5 9 4.0133806109569304E-5 4.0133806109569304E-5 0.0 0.0 4.0133806109569304E-5 10-11 8.026761221913861E-5 4.0133806109569304E-5 0.0 0.0 4.0133806109569304E-5 12-13 1.2040141832870791E-4 8.026761221913861E-5 0.0 2.0066903054784652E-5 4.0133806109569304E-5 14-15 2.207359336026312E-4 8.026761221913861E-5 0.0 4.0133806109569304E-5 4.0133806109569304E-5 16-17 2.4080283665741582E-4 8.026761221913861E-5 0.0 4.0133806109569304E-5 4.0133806109569304E-5 18-19 2.809366427669851E-4 8.026761221913861E-5 0.0 4.0133806109569304E-5 4.0133806109569304E-5 20-21 3.2107044887655443E-4 8.026761221913861E-5 0.0 4.0133806109569304E-5 4.0133806109569304E-5 22-23 3.2107044887655443E-4 8.026761221913861E-5 0.0 2.0066903054784653E-4 4.0133806109569304E-5 24-25 5.618732855339702E-4 8.026761221913861E-5 0.0 4.0133806109569306E-4 4.0133806109569304E-5 26-27 5.618732855339702E-4 8.026761221913861E-5 0.0 0.0011638803771775097 4.0133806109569304E-5 28-29 6.421408977531089E-4 8.026761221913861E-5 0.0 0.0032508382948751137 4.0133806109569304E-5 30-31 6.822747038626782E-4 8.026761221913861E-5 0.0 0.010414722685433234 4.0133806109569304E-5 32-33 7.023416069174629E-4 8.026761221913861E-5 0.0 0.02674918177202794 4.0133806109569304E-5 34-35 7.224085099722475E-4 8.026761221913861E-5 0.0 0.052434817682152295 4.0133806109569304E-5 36-37 7.625423160818168E-4 8.026761221913861E-5 0.0 0.09618066634158284 4.0133806109569304E-5 38-39 8.026761221913861E-4 8.026761221913861E-5 0.0 0.1609566294024277 4.0133806109569304E-5 40-41 8.026761221913861E-4 8.026761221913861E-5 0.0 0.23121085699722876 4.0133806109569304E-5 42-43 8.026761221913861E-4 8.026761221913861E-5 0.0 0.30391324676471354 4.0133806109569304E-5 44-45 8.428099283009553E-4 8.026761221913861E-5 0.0 0.39610059939839426 4.0133806109569304E-5 46-47 9.030106374653094E-4 8.026761221913861E-5 0.0 0.5042812737667384 4.0133806109569304E-5 48-49 9.23077540520094E-4 1.0033451527392327E-4 0.0 0.6230372060449538 8.026761221913861E-5 50-51 9.23077540520094E-4 1.6053522443827722E-4 0.0 0.7550372943393273 1.2040141832870791E-4 52-53 9.23077540520094E-4 1.8060212749306187E-4 0.0 0.8843082838182501 2.0066903054784653E-4 54-55 9.23077540520094E-4 2.4080283665741582E-4 0.0 1.0224689113504424 2.0066903054784653E-4 56-57 0.0010033451527392326 2.4080283665741582E-4 0.0 1.1877399249096487 2.0066903054784653E-4 58-59 0.0010033451527392326 2.4080283665741582E-4 0.0 1.3665360311277799 2.0066903054784653E-4 60-61 0.0010033451527392326 2.4080283665741582E-4 0.0 1.5584960257498501 2.0066903054784653E-4 62-63 0.0010033451527392326 2.4080283665741582E-4 0.0 1.7508774253360704 2.0066903054784653E-4 64-65 0.0010434789588488018 2.4080283665741582E-4 0.0 1.9545966251482443 2.0066903054784653E-4 66-67 0.0011638803771775099 2.4080283665741582E-4 0.0 2.1970048140500427 2.0066903054784653E-4 68-69 0.001204014183287079 2.4080283665741582E-4 0.0 2.4572123459614357 2.0066903054784653E-4 70-71 0.0012441479893966485 2.6086973971220045E-4 0.0 2.732229252327259 2.4080283665741582E-4 72-73 0.0012842817955062177 2.809366427669851E-4 0.0 3.0237411530041154 3.411373519313391E-4 74-75 0.001324415601615787 2.809366427669851E-4 0.0 3.3161761312214924 3.6120425498612375E-4 76-77 0.001324415601615787 2.809366427669851E-4 0.0 3.6377683195774715 3.6120425498612375E-4 78-79 0.001324415601615787 2.809366427669851E-4 0.0 3.9983705674719516 3.6120425498612375E-4 80-81 0.0015652184382732028 2.809366427669851E-4 0.0 4.371394228357344 4.0133806109569306E-4 82-83 0.0015652184382732028 2.809366427669851E-4 0.0 4.759769070079646 4.8160567331483165E-4 84-85 0.0016053522443827723 3.6120425498612375E-4 0.0 5.153923179881726 4.8160567331483165E-4 86-87 0.0016454860504923415 3.6120425498612375E-4 0.0 5.564371614964291 4.8160567331483165E-4 88-89 0.0016454860504923415 3.6120425498612375E-4 0.0 5.997756520238475 4.8160567331483165E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4025 0.0 49.984257 1 GTATCAA 6640 0.0 43.980385 1 ATCAACG 8385 0.0 34.59797 3 TATCAAC 8545 0.0 34.11711 2 TCAACGC 8500 0.0 34.017975 4 CAACGCA 8820 0.0 32.675922 5 AACGCAG 9135 0.0 31.523186 6 GTGGTAT 1845 0.0 29.903385 1 TGGTATC 1885 0.0 27.500397 2 ACGCAGA 10540 0.0 27.13803 7 CGCAGAG 10670 0.0 26.673796 8 TAAGGTG 1240 0.0 25.313143 5 GTACATG 8665 0.0 25.249224 1 TACATGG 8645 0.0 24.75548 2 GCAGAGT 11780 0.0 23.515038 9 ACATGGG 8885 0.0 23.39095 3 GAGTACT 6635 0.0 22.19883 12-13 CATGGGG 6345 0.0 21.211855 4 AGGTAAG 1315 0.0 20.976164 2 GTACTTT 7075 0.0 20.849123 14-15 >>END_MODULE