FastQCFastQC Report
Thu 26 May 2016
SRR938603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491665
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61500.2468229075738512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50110.2011105024150518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.1409499270568074No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31180.12513720744963708No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29990.12036128452259834No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28390.11393987554506725No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26920.10804020604696057No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25670.1030234802832644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.053.5971451
GTATCAA61350.043.304111
TATCAAC84100.031.476842
ATCAACG83950.031.4631983
TCAACGC85800.030.7847964
CAACGCA87450.030.2582745
GTGGTAT15900.030.1895161
AACGCAG90700.029.2129346
TGGTATC17700.026.0453622
ACGCAGA106300.024.9258067
CGCAGAG107600.024.5363988
GTACATG85100.023.2883321
GTGTAAG8050.023.0250531
TACATGG85350.022.8303322
TAAGGTG14600.022.451485
TAAGGTA7450.022.3182724
ACATGGG86000.021.7643933
GAGTACT71200.021.34117112-13
GCAGAGT120850.021.256859
GTACTTT73450.020.3965114-15
Produced by FastQC (version 0.11.5)