##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938603_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2491665 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79608374319983 31.0 31.0 34.0 30.0 34.0 2 32.02520041819426 31.0 31.0 34.0 30.0 34.0 3 32.08678132895072 33.0 31.0 34.0 30.0 34.0 4 35.76697950968529 37.0 35.0 37.0 35.0 37.0 5 35.657812346362775 37.0 35.0 37.0 33.0 37.0 6 35.65258050339833 37.0 35.0 37.0 33.0 37.0 7 35.590060862916964 37.0 35.0 37.0 33.0 37.0 8 35.56367168138574 37.0 35.0 37.0 33.0 37.0 9 37.145431267847 39.0 37.0 39.0 33.0 39.0 10-11 37.044437153469666 39.0 37.0 39.0 33.0 39.0 12-13 36.92952744449997 39.0 37.0 39.0 33.0 39.0 14-15 37.98421376870486 40.0 37.0 41.0 33.0 41.0 16-17 37.89733451326723 40.0 37.0 41.0 33.0 41.0 18-19 38.12059526461222 40.0 37.0 41.0 33.0 41.0 20-21 38.07978038781297 40.0 37.0 41.0 33.0 41.0 22-23 37.97286091830162 40.0 37.0 41.0 33.0 41.0 24-25 37.848051804716924 40.0 37.0 41.0 32.0 41.0 26-27 37.65683589086013 40.0 37.0 41.0 32.0 41.0 28-29 37.538959691611836 39.5 36.5 41.0 32.0 41.0 30-31 37.28439878555103 39.0 36.0 41.0 31.0 41.0 32-33 37.10293016918406 39.0 36.0 41.0 31.0 41.0 34-35 37.02205091775981 39.0 36.0 41.0 31.0 41.0 36-37 36.983131359954086 39.0 36.0 41.0 31.0 41.0 38-39 36.82407245757355 39.0 35.0 41.0 30.0 41.0 40-41 36.64585708752982 39.0 35.0 40.5 30.0 41.0 42-43 36.43720022555199 39.0 35.0 40.0 30.0 41.0 44-45 36.15232003499668 38.5 35.0 40.0 30.0 41.0 46-47 35.78644099427491 38.0 35.0 40.0 28.5 41.0 48-49 35.43450283244337 38.0 34.0 40.0 27.5 41.0 50-51 35.05131227512527 38.0 34.0 40.0 26.5 41.0 52-53 34.539846247388795 38.0 33.0 40.0 25.5 41.0 54-55 34.18136828185169 37.0 33.0 40.0 24.0 41.0 56-57 33.83815701548964 37.0 33.0 40.0 24.0 41.0 58-59 33.34083795373776 36.0 32.0 39.0 22.5 40.5 60-61 32.92762690811165 36.0 31.0 39.0 22.0 40.0 62-63 32.428857410606966 35.0 31.0 39.0 20.5 40.0 64-65 31.890035377950085 35.0 30.5 38.0 18.5 40.0 66-67 31.368548942173206 35.0 30.0 38.0 17.5 40.0 68-69 30.862418703959 34.0 29.0 37.0 13.5 39.0 70-71 30.27720640615813 34.0 29.0 36.5 9.5 39.0 72-73 29.765289073771953 34.0 28.5 36.0 7.5 39.0 74-75 29.166369475832425 33.5 27.5 35.5 7.0 38.0 76-77 27.976096305081143 31.5 26.0 34.5 4.0 36.0 78-79 28.6683597112774 33.0 27.5 35.0 2.0 37.0 80-81 28.30184575374298 33.0 27.0 35.0 2.0 36.5 82-83 27.863299641003103 33.0 26.0 35.0 2.0 36.0 84-85 27.298043075614096 32.5 26.0 35.0 2.0 35.5 86-87 26.68018373256437 32.0 25.0 34.5 2.0 35.0 88-89 25.925979014032784 31.5 23.5 34.0 2.0 35.0 90-91 25.315845227990117 31.0 21.0 34.0 2.0 35.0 92-93 24.47473376236372 31.0 18.0 34.0 2.0 35.0 94-95 23.634974806003214 30.5 8.5 34.0 2.0 35.0 96-97 22.54188042935146 30.0 2.0 34.0 2.0 35.0 98-99 21.152344516618406 29.0 2.0 34.0 2.0 35.0 100-101 17.244669728876072 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23450012404500598 1101 2 0.2624413751790726 1101 3 0.28178080097257663 1101 4 0.15691012597331166 1101 5 -0.00934179276337943 1101 6 0.14208186908355458 1101 7 0.1580443333123256 1101 8 0.20787016167296457 1101 9 0.2553131645027449 1101 10-11 0.20326807577722406 1101 12-13 0.3429141653319334 1101 14-15 0.4282552926162353 1101 16-17 0.36313023593096716 1101 18-19 0.3611823133595635 1101 20-21 0.33747045021468836 1101 22-23 0.29975876053874373 1101 24-25 0.38939029284311744 1101 26-27 0.3634626700667738 1101 28-29 0.2647277576280871 1101 30-31 0.3911972914534232 1101 32-33 0.3707101262786523 1101 34-35 0.25103556495250956 1101 36-37 0.3045441526008972 1101 38-39 0.43064122392163284 1101 40-41 0.47513060062958345 1101 42-43 0.43511327499592056 1101 44-45 0.4710403816338129 1101 46-47 0.4638706548549649 1101 48-49 0.606353220689499 1101 50-51 0.7484307943653548 1101 52-53 0.7859457148297082 1101 54-55 0.7726087960326922 1101 56-57 0.500569294745226 1101 58-59 0.587038570651174 1101 60-61 0.7355396663703502 1101 62-63 0.7881369278066117 1101 64-65 0.641404214054031 1101 66-67 0.607237895699992 1101 68-69 0.525986315649849 1101 70-71 0.4065965198979633 1101 72-73 0.503889389863307 1101 74-75 0.4666026171344484 1101 76-77 0.36007431648117816 1101 78-79 0.5615086056399186 1101 80-81 0.7556341593381362 1101 82-83 0.7397837090265291 1101 84-85 0.8852522225216646 1101 86-87 0.9155550237624048 1101 88-89 0.7388659587384332 1101 90-91 0.9353838934032481 1101 92-93 1.1347026982374615 1101 94-95 1.1282738252405835 1101 96-97 1.2797455390074042 1101 98-99 1.3872419756584797 1101 100-101 1.697025796333424 1102 1 0.20403888104618773 1102 2 0.23468233145059259 1102 3 0.1442170516791208 1102 4 0.15413663026394175 1102 5 0.12100913344801967 1102 6 0.1445717848124488 1102 7 0.17577595039264082 1102 8 0.20075191877691623 1102 9 0.1405658814751476 1102 10-11 0.27067548675301367 1102 12-13 0.34280585576408384 1102 14-15 0.4092579228767548 1102 16-17 0.39628324065837006 1102 18-19 0.33514987990290734 1102 20-21 0.25737652839668357 1102 22-23 0.2822220846228092 1102 24-25 0.3394124525471369 1102 26-27 0.292942623563512 1102 28-29 0.2708165377524381 1102 30-31 0.2935679783400147 1102 32-33 0.28738963915055393 1102 34-35 0.32303742122916645 1102 36-37 0.5584346258550923 1102 38-39 0.5327029595724184 1102 40-41 0.4041685643775921 1102 42-43 0.3983073715223142 1102 44-45 0.4367338679654509 1102 46-47 0.5661354408371935 1102 48-49 0.6359858031621357 1102 50-51 0.7745558810772408 1102 52-53 0.6906611553260902 1102 54-55 0.4771548271975874 1102 56-57 0.6685021155149755 1102 58-59 0.8166298044007263 1102 60-61 0.6317047529537732 1102 62-63 0.6203735163683461 1102 64-65 0.5840975333849947 1102 66-67 0.501875648770234 1102 68-69 0.5795148470716676 1102 70-71 0.5695805006800434 1102 72-73 0.7583877294912824 1102 74-75 0.8888274353606427 1102 76-77 0.7938335818066982 1102 78-79 1.129720349640742 1102 80-81 1.184496692095955 1102 82-83 1.001764080024934 1102 84-85 1.0532532887567 1102 86-87 0.9810232197698028 1102 88-89 1.0274650318847272 1102 90-91 1.1021519648024558 1102 92-93 1.5547326620111654 1102 94-95 1.3483167155083038 1102 96-97 1.3090531927764815 1102 98-99 1.3876235079538404 1102 100-101 1.5235755304169736 1103 1 0.31760245851211977 1103 2 0.22992442200085605 1103 3 0.27485884467375143 1103 4 0.21724861061969136 1103 5 0.13723089356694373 1103 6 0.23344496726249986 1103 7 0.27528205370760617 1103 8 0.267407836151321 1103 9 0.2956396425478687 1103 10-11 0.31722325166123966 1103 12-13 0.37787621447969855 1103 14-15 0.49182556731680194 1103 16-17 0.45671820331805435 1103 18-19 0.5001761696911373 1103 20-21 0.4938765746915763 1103 22-23 0.4000003993367258 1103 24-25 0.5956308705557234 1103 26-27 0.556942229105708 1103 28-29 0.5189117885312058 1103 30-31 0.3350641307909825 1103 32-33 0.4403682394975732 1103 34-35 0.5830230063965374 1103 36-37 0.468469708500983 1103 38-39 0.39873578264731435 1103 40-41 0.5454195758891629 1103 42-43 0.5685675202493954 1103 44-45 0.5726906152232729 1103 46-47 0.5466142187536391 1103 48-49 0.6464456731052053 1103 50-51 0.7979610380739857 1103 52-53 0.7144901007919771 1103 54-55 0.7290791566193207 1103 56-57 0.735286453637606 1103 58-59 0.8101929125760208 1103 60-61 0.8801767045939997 1103 62-63 0.7311131057263651 1103 64-65 0.7934820935962499 1103 66-67 0.8227134054848939 1103 68-69 0.6931532573686674 1103 70-71 0.5036905100151223 1103 72-73 0.6603165298575817 1103 74-75 0.741806685738613 1103 76-77 0.6512179809371155 1103 78-79 0.9088407848380449 1103 80-81 0.9040742924734175 1103 82-83 0.899220271109936 1103 84-85 1.0115209848187163 1103 86-87 1.0523083880295232 1103 88-89 0.9893547479512073 1103 90-91 1.0848098525633567 1103 92-93 1.3276719370712833 1103 94-95 1.2284976999785862 1103 96-97 1.257925563480569 1103 98-99 1.4839866323640543 1103 100-101 1.4576828445017824 1104 1 0.2351070121313228 1104 2 0.27240923160704966 1104 3 0.2693395952030926 1104 4 0.19112453224406778 1104 5 0.08928281751430944 1104 6 0.083300957084667 1104 7 0.15576414136994998 1104 8 0.2611789557087576 1104 9 0.20987241903979736 1104 10-11 0.21742043802210986 1104 12-13 0.2717573160718132 1104 14-15 0.3293202285510617 1104 16-17 0.4758360984916905 1104 18-19 0.35294196947707945 1104 20-21 0.37193393606707303 1104 22-23 0.4213365449454969 1104 24-25 0.43774405438875164 1104 26-27 0.36621944673710516 1104 28-29 0.47417681509007537 1104 30-31 0.43540416614334276 1104 32-33 0.5042122646401026 1104 34-35 0.5047850382481087 1104 36-37 0.3756395497691116 1104 38-39 0.30479403790560156 1104 40-41 0.5006040115010748 1104 42-43 0.5736023012411877 1104 44-45 0.44419757826570816 1104 46-47 0.4876664781377613 1104 48-49 0.5084551314679118 1104 50-51 0.6387303986263504 1104 52-53 0.6439867872582781 1104 54-55 0.6242052653023649 1104 56-57 0.5629497883402692 1104 58-59 0.6844571933610339 1104 60-61 0.7246917754855815 1104 62-63 0.71817072256286 1104 64-65 0.6946793613170748 1104 66-67 0.6840878256414946 1104 68-69 0.8030413974418842 1104 70-71 0.7565869009710333 1104 72-73 0.6796658164046967 1104 74-75 0.8976579652967658 1104 76-77 0.9470248985625567 1104 78-79 1.124667570285105 1104 80-81 0.8803480254691536 1104 82-83 1.126466363102658 1104 84-85 1.2159135451669556 1104 86-87 1.3898543414027387 1104 88-89 1.2599605381031473 1104 90-91 1.3911718003481148 1104 92-93 1.7069116322344904 1104 94-95 1.6283555277049793 1104 96-97 1.5455242008783685 1104 98-99 1.8978442918337741 1104 100-101 2.1112561225124047 1105 1 0.058746558201917765 1105 2 0.11616559831081474 1105 3 0.12853370441575862 1105 4 0.10616723722210963 1105 5 2.0278691771125068E-5 1105 6 0.11482231757489814 1105 7 0.052836128110463676 1105 8 0.0918986838335627 1105 9 0.1709136372744382 1105 10-11 0.05071714853195175 1105 12-13 0.14844081145681542 1105 14-15 0.2500503123163256 1105 16-17 0.18108188455799734 1105 18-19 0.13241750199836844 1105 20-21 0.18190548880119906 1105 22-23 0.1610788081826442 1105 24-25 0.0966643147718429 1105 26-27 0.15156460783480696 1105 28-29 0.19678730180221748 1105 30-31 0.2025224961900065 1105 32-33 0.2763922491977624 1105 34-35 0.23129826343372173 1105 36-37 0.27746047705307575 1105 38-39 0.09762551656333329 1105 40-41 0.18823809659750168 1105 42-43 0.30814244427615023 1105 44-45 0.1788103248752364 1105 46-47 0.07743220418313257 1105 48-49 0.2618067124996486 1105 50-51 0.20750502364739987 1105 52-53 0.23018004700912797 1105 54-55 0.15518691452663802 1105 56-57 0.17175535730114433 1105 58-59 0.40848122721859426 1105 60-61 0.0830391640313195 1105 62-63 0.032362321025374285 1105 64-65 -0.009490484225491258 1105 66-67 0.10515114499690625 1105 68-69 0.09374210935697747 1105 70-71 0.307254564924353 1105 72-73 0.2540478081104034 1105 74-75 0.2730314106727221 1105 76-77 0.3686065926232409 1105 78-79 0.5944226991720072 1105 80-81 0.48511676159946404 1105 82-83 0.48991133752026883 1105 84-85 0.5003207125325666 1105 86-87 0.5485336764547526 1105 88-89 0.45252996230790643 1105 90-91 0.7350017337350749 1105 92-93 0.7456569743077104 1105 94-95 1.1530786006320106 1105 96-97 1.0990149585174116 1105 98-99 1.0576174960473317 1105 100-101 1.108661878257088 1106 1 0.02813129826046179 1106 2 0.10072327333952558 1106 3 0.027803654565346392 1106 4 0.012386211503475408 1106 5 0.05163381132458511 1106 6 9.007050546898654E-4 1106 7 0.08759465932190125 1106 8 0.06964969290395828 1106 9 -0.14037608798754775 1106 10-11 0.12398651303828956 1106 12-13 0.03817070082270391 1106 14-15 0.10049958793280211 1106 16-17 0.15323104055529058 1106 18-19 0.024158826881382822 1106 20-21 0.17854990328394393 1106 22-23 0.16060840726164116 1106 24-25 0.2651106550983613 1106 26-27 0.2242437606592489 1106 28-29 0.17647060939269466 1106 30-31 0.24534710635187196 1106 32-33 0.1066783906001092 1106 34-35 0.151085916912713 1106 36-37 0.25965708430117473 1106 38-39 0.14754902483462473 1106 40-41 0.12543817364186083 1106 42-43 0.17484530961018407 1106 44-45 0.029458106143515295 1106 46-47 -0.4778505145809291 1106 48-49 -0.3209412600384809 1106 50-51 -0.042193128393194 1106 52-53 0.1251865467060611 1106 54-55 0.2677051348254693 1106 56-57 0.22273763606644792 1106 58-59 0.1927572364326764 1106 60-61 0.24527339745834098 1106 62-63 0.09898049356201 1106 64-65 0.24907808518245034 1106 66-67 0.11031347089366861 1106 68-69 -0.028956194031284355 1106 70-71 0.05400180592421222 1106 72-73 0.4530439029869662 1106 74-75 0.42073074195772975 1106 76-77 0.33011978042801005 1106 78-79 0.5375809668292497 1106 80-81 -0.004295828197818707 1106 82-83 0.5554298447459765 1106 84-85 0.5543364288159722 1106 86-87 0.9501869470601036 1106 88-89 0.9712132199403918 1106 90-91 0.68887665395334 1106 92-93 0.7993028688144115 1106 94-95 1.0227032716866304 1106 96-97 1.2221402627416893 1106 98-99 1.041131432190408 1106 100-101 1.1538362088089364 1107 1 0.22491984198556025 1107 2 0.19194299524163227 1107 3 0.17387751777066285 1107 4 0.15314334006504282 1107 5 0.10881302153812555 1107 6 0.10719190223397135 1107 7 0.0984035564314567 1107 8 0.10167047257513673 1107 9 0.2371227650121739 1107 10-11 0.1850630676300824 1107 12-13 0.22173236247387962 1107 14-15 0.3913811705403063 1107 16-17 0.28704872711895035 1107 18-19 0.17862092890841552 1107 20-21 0.238368695302988 1107 22-23 0.3089633379178025 1107 24-25 0.2293738112818673 1107 26-27 0.2902864110065977 1107 28-29 0.3182107984761373 1107 30-31 0.2736654052511156 1107 32-33 0.2975406096753517 1107 34-35 0.3986968688687469 1107 36-37 0.4071280119082985 1107 38-39 0.2606586419352297 1107 40-41 0.23407503154749065 1107 42-43 0.28645670952854374 1107 44-45 0.23600095699013224 1107 46-47 0.34537459731934206 1107 48-49 0.36329776398169145 1107 50-51 0.34281545153015003 1107 52-53 0.3138160349091166 1107 54-55 0.17296052753307833 1107 56-57 0.3126656369623717 1107 58-59 0.2533000656037032 1107 60-61 0.34340886270694426 1107 62-63 0.45346571622457077 1107 64-65 0.3801909463972173 1107 66-67 0.24606021323225136 1107 68-69 0.3595558846944833 1107 70-71 0.6131172248592911 1107 72-73 0.553614260164661 1107 74-75 0.3343283489719546 1107 76-77 0.5415183259955363 1107 78-79 0.8496104167387521 1107 80-81 0.8961420910894873 1107 82-83 0.9463411229197334 1107 84-85 1.0295584049800404 1107 86-87 1.3101535666425583 1107 88-89 1.1997226253426696 1107 90-91 1.3122284235851076 1107 92-93 1.388557697956525 1107 94-95 1.6170101764476748 1107 96-97 1.720060561575906 1107 98-99 2.046088640951684 1107 100-101 1.9053489847663627 1108 1 0.16051915239296122 1108 2 0.09858667548843414 1108 3 0.13664314702390357 1108 4 0.1678570684018581 1108 5 0.06618168136935765 1108 6 0.13050376141190156 1108 7 0.03751978907822462 1108 8 0.05471647566015747 1108 9 0.07755103830860577 1108 10-11 0.1024571335043234 1108 12-13 0.2038356198915352 1108 14-15 0.283053432585497 1108 16-17 0.23264168601560442 1108 18-19 0.205199279330337 1108 20-21 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-0.5812061272094589 2316 72-73 -0.7225339139541731 2316 74-75 -0.6117946619880819 2316 76-77 -0.8748064650114706 2316 78-79 -0.8220721549433812 2316 80-81 -0.6235309704525136 2316 82-83 -0.9062528737130044 2316 84-85 -0.9515346767947506 2316 86-87 -1.1428070305533389 2316 88-89 -1.0333222629570074 2316 90-91 -1.036207041495075 2316 92-93 -1.297979391224775 2316 94-95 -1.2654223596262142 2316 96-97 -1.3766742172695103 2316 98-99 -1.462719925478691 2316 100-101 -1.799707020609567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 49.0 5 68.0 6 179.0 7 441.0 8 1111.0 9 1913.0 10 3205.0 11 4856.0 12 6787.0 13 9391.0 14 12461.0 15 16481.0 16 20598.0 17 25200.0 18 24460.0 19 22737.0 20 23069.0 21 24620.0 22 26907.0 23 30447.0 24 35056.0 25 40953.0 26 48201.0 27 58166.0 28 69174.0 29 82509.0 30 97101.0 31 115190.0 32 138839.0 33 169461.0 34 214068.0 35 276406.0 36 353998.0 37 372431.0 38 159360.0 39 5755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49970401199203 18.731044225625137 14.91623037699842 23.853021385384412 2 16.330186156436937 20.024766250442685 38.440047444555 25.205000148565382 3 19.49302976122392 23.19441016348506 29.456327395536718 27.8562326797543 4 13.997146486385608 16.382017646834544 35.08958868868808 34.53124717809175 5 14.535331224837474 36.701077950773296 33.5584902827036 15.20510054168562 6 34.03815042403407 34.996185278667305 16.403783336671133 14.561880960627501 7 30.15810712916865 31.7457603650571 19.56282245004846 18.533310055725792 8 27.307162078369284 34.695595114110446 18.824159748601836 19.173083058918433 9 26.592338857751745 16.680372361453085 19.17521015064224 37.55207863015293 10-11 25.80878649417157 25.965589274641655 26.582064603387696 21.643559627799082 12-13 26.408124687708824 22.97517924761154 27.113355928666174 23.50334013601347 14-15 22.936710994455513 24.687508152179365 25.030451525385633 27.345329327979485 16-17 23.12722617205764 27.937182566677304 25.865274826270785 23.07031643499427 18-19 23.230570722789782 26.342204911173855 27.853623982357178 22.57360038367919 20-21 23.445627722827908 25.535294672437907 28.203048965250144 22.81602863948404 22-23 23.34770123592056 25.28803029299685 28.21729646641904 23.14697200466355 24-25 23.34537560074204 25.628998090308574 28.047478990584796 22.978147318364588 26-27 22.735694595422775 26.119256973225646 27.85485504508212 23.290193386269454 28-29 22.91060227647158 26.363657525114753 27.890890541778724 22.834849656634944 30-31 22.972860433601344 25.88219193091404 27.830682882886858 23.314264752597758 32-33 22.75570765443356 25.831696535714833 27.760937112456226 23.651658697395384 34-35 23.454103565508134 25.99900857760991 27.52084194639897 23.02604591048299 36-37 23.60488445993792 25.60618188708209 27.52289766222255 23.266035990757437 38-39 23.01436092268557 25.863828704426517 27.620965718971256 23.50084465391665 40-41 23.294910794572164 25.69542146296219 27.76113209129823 23.248535651167415 42-43 23.200737508588748 25.751354935239235 27.471412311288347 23.576495244883674 44-45 23.15303411546123 25.92844491021497 27.625889491705834 23.29263148261796 46-47 23.496597656587063 26.21829178481056 27.030720421886567 23.25439013671581 48-49 23.36347382172162 26.083341861767135 27.005275588813106 23.547908727698147 50-51 23.495195155663794 26.345894209945854 26.9575968072588 23.20131382713156 52-53 23.772557707396462 26.045254879769153 26.915415996933778 23.266771415900614 54-55 23.21747389405029 26.278467610802668 27.024446490855063 23.479612004291976 56-57 23.276624265300512 26.3956631409118 27.01898128359952 23.308731310188165 58-59 23.302989727947264 25.927835703356717 26.90472142699581 23.864453141700213 60-61 22.936470191618856 26.279796842673477 27.31835941027385 23.465373555433818 62-63 23.032712761794212 26.376799029305857 27.1491920448356 23.44129616406433 64-65 22.75875259831865 26.307237520393677 27.072347908670842 23.861661972616826 66-67 22.783737615854275 26.286518279007115 27.079637422074804 23.850106683063803 68-69 23.162106122589883 25.893134005675485 27.254262583535155 23.690497288199477 70-71 22.968716074364792 26.18773084482131 26.87179205213546 23.971761028678436 72-73 23.046365802130627 26.251071573053185 26.71315233514631 23.989410289669873 74-75 22.917563608884016 26.695730103438137 26.563198423188126 23.823507864489716 76-77 23.019975269077776 26.24145997166324 26.833552541364263 23.905012217894722 78-79 23.094261632833064 26.34351998069767 26.951535223433055 23.610683163036207 80-81 23.187805374470244 26.54196167882845 26.63262811426259 23.637604832438715 82-83 23.12140770563702 26.536446079651576 26.60405629152367 23.738089923187733 84-85 22.98970719163923 26.460629867528564 26.689048968912843 23.86061397191936 86-87 23.128009737526302 26.43479499918218 26.519506661435322 23.91768860185619 88-89 22.956887337334862 26.611789213057634 26.315671083936152 24.115652365671345 90-91 22.936626712452497 26.623894327539986 26.475601541217287 23.96387741879023 92-93 23.054595451717148 26.134842736998454 26.585656650809895 24.2249051604745 94-95 22.715970183766146 26.07828527751193 26.77624014850309 24.42950439021884 96-97 22.899619103522177 25.821344415612778 26.777640690198712 24.50139579066633 98-99 22.661897297814974 26.162448327766146 26.377800751023006 24.797853623395874 100-101 22.79977939290501 25.884686230962302 26.772633802257207 24.542900573875478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 380.0 1 271.5 2 210.5 3 269.0 4 257.0 5 274.5 6 488.5 7 1030.0 8 1659.0 9 1848.0 10 1620.5 11 1451.5 12 1479.0 13 1579.5 14 1792.5 15 1987.5 16 2071.5 17 2202.5 18 2430.0 19 2676.5 20 3009.5 21 3516.0 22 4237.0 23 5135.0 24 6195.5 25 7866.5 26 10208.5 27 12819.0 28 15419.5 29 18547.0 30 22046.5 31 26909.5 32 34788.5 33 38377.5 34 38061.0 35 41067.0 36 45778.5 37 49706.0 38 53531.5 39 56503.5 40 59909.5 41 67055.0 42 72516.5 43 76625.5 44 81922.5 45 88500.5 46 93397.0 47 95997.0 48 96625.0 49 95330.0 50 95920.5 51 95353.0 52 94975.5 53 97029.0 54 98856.5 55 94908.5 56 87150.0 57 80693.5 58 75112.5 59 66851.5 60 54367.0 61 44540.5 62 38087.5 63 29626.0 64 22161.0 65 17306.0 66 12776.5 67 9794.0 68 7479.0 69 5361.0 70 4028.5 71 2979.5 72 2307.5 73 1908.0 74 1484.0 75 1156.5 76 802.0 77 485.0 78 307.0 79 186.0 80 105.0 81 69.0 82 47.0 83 28.0 84 12.5 85 7.0 86 5.0 87 3.0 88 1.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.0133806109569306E-4 2 0.04731775740318221 3 0.0 4 0.0 5 0.04438798955718365 6 8.026761221913861E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0065418103958597975 26-27 0.0018260881779854033 28-29 8.026761221913861E-5 30-31 0.0017859543718758341 32-33 4.414718672052624E-4 34-35 0.011839472802322944 36-37 0.023317741349659766 38-39 0.004494986284271763 40-41 0.001143813474122725 42-43 0.0013244156016157871 44-45 0.008588634507447832 46-47 0.0 48-49 0.0 50-51 5.21739479424401E-4 52-53 0.0 54-55 0.0015852853413279874 56-57 0.0 58-59 0.023498343477152826 60-61 0.0 62-63 0.010113719139611465 64-65 0.015672251285786813 66-67 0.00694314845695549 68-69 0.0169966668874026 70-71 8.026761221913861E-5 72-73 4.0133806109569304E-5 74-75 0.022575265936632734 76-77 0.05058866260111211 78-79 0.031043499025751858 80-81 0.026729114868973156 82-83 0.03680270020247505 84-85 0.016494994311032983 86-87 0.010936462164857635 88-89 0.08500340134006779 90-91 0.025966572552891337 92-93 0.02468229075738512 94-95 0.027010051511740144 96-97 0.03860872147740567 98-99 0.05331776141656282 100-101 0.01436790258722581 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2491665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.08657230094142 #Duplication Level Percentage of deduplicated Percentage of total 1 73.73797754149813 36.195445659159425 2 13.896844000454436 13.642968755664214 3 5.026214188735128 7.401588785260936 4 2.309294420775532 4.534213901982351 5 1.2882466114321005 3.1617805216752295 6 0.7970607888036302 2.347498922271287 7 0.5417808438432674 1.8615915193804318 8 0.39703782185349645 1.5591380598895965 9 0.2832241566597016 1.251225273892464 >10 1.5320972191939417 13.481172206221395 >50 0.11553154848351688 3.9091034775265348 >100 0.0672378165162383 6.194832245329371 >500 0.004095077885154986 1.3808008123746516 >1k 0.0031941607504196176 2.6299378892181076 >5k 1.6380311540613426E-4 0.44870197015398594 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6150 0.2468229075738512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5011 0.2011105024150518 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3512 0.1409499270568074 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3118 0.12513720744963708 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2999 0.12036128452259834 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2839 0.11393987554506725 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2692 0.10804020604696057 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2567 0.1030234802832644 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.6053522443827722E-4 0.0 0.0 0.0 0.0 8 1.6053522443827722E-4 0.0 0.0 0.0 0.0 9 1.6053522443827722E-4 0.0 0.0 0.0 0.0 10-11 1.6053522443827722E-4 0.0 0.0 0.0 4.0133806109569304E-5 12-13 1.6053522443827722E-4 0.0 0.0 2.0066903054784652E-5 4.0133806109569304E-5 14-15 2.809366427669851E-4 0.0 0.0 6.0200709164353956E-5 4.0133806109569304E-5 16-17 2.809366427669851E-4 0.0 0.0 8.026761221913861E-5 4.0133806109569304E-5 18-19 2.809366427669851E-4 0.0 0.0 8.026761221913861E-5 4.0133806109569304E-5 20-21 2.809366427669851E-4 0.0 0.0 8.026761221913861E-5 4.0133806109569304E-5 22-23 3.6120425498612375E-4 0.0 0.0 2.4080283665741582E-4 6.0200709164353956E-5 24-25 4.4147186720526233E-4 0.0 0.0 4.414718672052624E-4 1.6053522443827722E-4 26-27 4.4147186720526233E-4 0.0 0.0 0.001204014183287079 1.6053522443827722E-4 28-29 5.618732855339702E-4 0.0 0.0 0.003290972100984683 1.6053522443827722E-4 30-31 6.421408977531089E-4 0.0 0.0 0.010595324812926295 1.6053522443827722E-4 32-33 6.421408977531089E-4 0.0 0.0 0.027010051511740144 1.6053522443827722E-4 34-35 6.822747038626782E-4 4.0133806109569304E-5 0.0 0.053016757870741046 1.6053522443827722E-4 36-37 7.224085099722475E-4 4.0133806109569304E-5 0.0 0.097003409366829 1.6053522443827722E-4 38-39 7.224085099722475E-4 8.026761221913861E-5 0.0 0.1619800414582217 1.6053522443827722E-4 40-41 7.224085099722475E-4 8.026761221913861E-5 0.0 0.23249513879273498 1.6053522443827722E-4 42-43 7.826092191366014E-4 1.0033451527392327E-4 0.0 0.3055185990090963 1.6053522443827722E-4 44-45 9.632113466296633E-4 1.6053522443827722E-4 0.0 0.39800695518859874 1.6053522443827722E-4 46-47 0.0010635458619035866 1.6053522443827722E-4 0.0 0.5072110416127369 1.6053522443827722E-4 48-49 0.0010836127649583712 2.0066903054784653E-4 0.0 0.6262077767276099 1.6053522443827722E-4 50-51 0.0010836127649583712 2.0066903054784653E-4 0.0 0.7582881326342025 1.6053522443827722E-4 52-53 0.0010836127649583712 2.207359336026312E-4 0.0 0.8876795235314539 1.8060212749306187E-4 54-55 0.0010836127649583712 2.4080283665741582E-4 0.0 1.0258802848697557 2.0066903054784653E-4 56-57 0.0010836127649583712 2.4080283665741582E-4 0.0 1.191733238617551 2.0066903054784653E-4 58-59 0.0010836127649583712 2.4080283665741582E-4 0.0 1.3706096124479012 2.0066903054784653E-4 60-61 0.0010836127649583712 2.4080283665741582E-4 0.0 1.5619675999783276 2.0066903054784653E-4 62-63 0.0011237465710679405 2.6086973971220045E-4 0.0 1.7547904714317535 2.0066903054784653E-4 64-65 0.0011237465710679405 2.809366427669851E-4 0.0 1.9582889353103248 2.0066903054784653E-4 66-67 0.001204014183287079 2.809366427669851E-4 0.0 2.202302476456506 2.0066903054784653E-4 68-69 0.001204014183287079 2.809366427669851E-4 0.0 2.463473219714528 2.0066903054784653E-4 70-71 0.0012441479893966485 2.809366427669851E-4 0.0 2.738871397238393 2.4080283665741582E-4 72-73 0.0012642148924514331 2.809366427669851E-4 0.0 3.0319485163535225 2.809366427669851E-4 74-75 0.0012842817955062177 2.809366427669851E-4 0.0 3.3270925264832956 2.809366427669851E-4 76-77 0.0012842817955062177 2.809366427669851E-4 0.0 3.65231682429219 2.809366427669851E-4 78-79 0.0012842817955062177 2.809366427669851E-4 0.0 4.020323759413886 2.809366427669851E-4 80-81 0.0015250846321636336 2.809366427669851E-4 0.0 4.402036389321999 2.809366427669851E-4 82-83 0.0015451515352184182 2.809366427669851E-4 0.0 4.800223946638091 2.809366427669851E-4 84-85 0.001665552953547126 2.809366427669851E-4 0.0 5.20565565595696 2.809366427669851E-4 86-87 0.0017658874688210493 2.809366427669851E-4 0.0 5.628605771642656 3.2107044887655443E-4 88-89 0.0017658874688210493 2.809366427669851E-4 0.0 6.075596037187985 3.6120425498612375E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3875 0.0 53.597145 1 GTATCAA 6135 0.0 43.30411 1 TATCAAC 8410 0.0 31.47684 2 ATCAACG 8395 0.0 31.463198 3 TCAACGC 8580 0.0 30.784796 4 CAACGCA 8745 0.0 30.258274 5 GTGGTAT 1590 0.0 30.189516 1 AACGCAG 9070 0.0 29.212934 6 TGGTATC 1770 0.0 26.045362 2 ACGCAGA 10630 0.0 24.925806 7 CGCAGAG 10760 0.0 24.536398 8 GTACATG 8510 0.0 23.288332 1 GTGTAAG 805 0.0 23.025053 1 TACATGG 8535 0.0 22.830332 2 TAAGGTG 1460 0.0 22.45148 5 TAAGGTA 745 0.0 22.318272 4 ACATGGG 8600 0.0 21.764393 3 GAGTACT 7120 0.0 21.341171 12-13 GCAGAGT 12085 0.0 21.25685 9 GTACTTT 7345 0.0 20.39651 14-15 >>END_MODULE