FastQCFastQC Report
Thu 26 May 2016
SRR938602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502220
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61610.2462213554363725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47610.19027103931708644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33700.13468040380142432No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30930.12361023411210845No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30700.12269105034729161No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29030.11601697692449106No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27340.10926297447866294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.051.5296751
GTATCAA60200.048.4125861
ATCAACG80400.035.766513
TATCAAC80800.035.4717942
TCAACGC80950.035.4060634
CAACGCA83550.034.2439465
AACGCAG88300.032.449816
GTGGTAT15850.029.4442251
ACGCAGA103650.027.3619927
GTACATG86900.027.2905881
CGCAGAG103600.027.283498
TACATGG86950.026.6217482
TAAGGTG13300.025.7285085
ACATGGG90750.024.7737483
TGGTATC17100.024.738372
GCAGAGT116300.024.0994059
CATGGGG61800.022.9964034
GAGTACT64800.021.33129712-13
AGAGTAC109350.020.22149310-11
CAGAGTA108650.020.15503310-11
Produced by FastQC (version 0.11.5)