##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938602_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2502220 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.103635571612408 31.0 31.0 34.0 28.0 34.0 2 31.189234759533534 31.0 31.0 34.0 28.0 34.0 3 31.259508756224474 31.0 31.0 34.0 27.0 34.0 4 34.89901367585584 37.0 35.0 37.0 32.0 37.0 5 34.90673561877053 37.0 35.0 37.0 32.0 37.0 6 34.93936943993734 37.0 35.0 37.0 32.0 37.0 7 34.86113611113331 37.0 35.0 37.0 32.0 37.0 8 34.820364716132076 37.0 35.0 37.0 32.0 37.0 9 36.330745098352665 39.0 35.0 39.0 32.0 39.0 10-11 36.175044360607785 39.0 35.0 39.0 31.0 39.0 12-13 36.028868964359646 38.5 35.0 39.0 30.0 39.0 14-15 37.03170005035528 39.0 36.0 41.0 31.0 41.0 16-17 36.88340493641646 39.0 36.0 41.0 31.0 41.0 18-19 36.85648783879915 39.0 36.0 41.0 30.5 41.0 20-21 36.933082622631105 39.0 36.0 41.0 30.5 41.0 22-23 36.86013659870035 39.0 36.0 41.0 30.0 41.0 24-25 36.739349457681584 39.0 36.0 41.0 30.0 41.0 26-27 36.54468272174309 39.0 36.0 41.0 30.0 41.0 28-29 36.35986763753787 39.0 35.5 41.0 29.5 41.0 30-31 36.14422053216744 39.0 35.0 40.0 29.0 41.0 32-33 35.87554411682426 38.0 35.0 40.0 27.5 41.0 34-35 35.62872049619938 38.0 34.5 40.0 27.0 41.0 36-37 35.341282541103496 38.0 34.0 40.0 26.0 41.0 38-39 35.08407773896779 38.0 34.0 40.0 25.5 41.0 40-41 35.02249342583785 38.0 34.0 40.0 25.0 41.0 42-43 34.8489149635124 38.0 33.5 40.0 24.5 41.0 44-45 34.56518191845641 38.0 33.0 40.0 24.0 41.0 46-47 34.21065873504328 38.0 33.0 40.0 23.5 41.0 48-49 33.84822697444669 38.0 33.0 40.0 22.5 41.0 50-51 33.09382828048693 37.0 31.5 39.5 20.5 40.5 52-53 33.14465554587527 37.0 32.0 39.5 20.5 40.0 54-55 33.26262958492859 37.0 32.0 40.0 19.5 41.0 56-57 32.950409836065575 37.0 32.0 40.0 18.5 41.0 58-59 32.60142073838431 36.5 31.0 39.5 16.0 41.0 60-61 32.14647732813262 36.0 31.0 39.0 13.0 41.0 62-63 31.609172255037528 35.0 30.5 39.0 8.0 40.0 64-65 31.099550798890583 35.0 29.5 38.5 7.5 40.0 66-67 30.55096334455004 35.0 29.0 38.0 4.0 40.0 68-69 29.985346412385802 34.0 28.5 37.5 2.0 39.5 70-71 29.347553372605127 34.0 28.0 37.0 2.0 39.0 72-73 28.80420286785335 34.0 26.5 36.0 2.0 39.0 74-75 28.195327748958924 33.5 26.0 36.0 2.0 38.5 76-77 27.472532391236584 33.0 25.5 35.0 2.0 37.5 78-79 26.696656968611872 32.0 24.0 35.0 2.0 37.0 80-81 26.056943034585288 32.0 22.0 35.0 2.0 36.0 82-83 25.402922205081886 31.0 20.0 34.5 2.0 36.0 84-85 24.761274987810822 31.0 18.0 34.0 2.0 35.0 86-87 24.0993571708323 31.0 12.5 34.0 2.0 35.0 88-89 23.312990664290112 30.0 4.5 34.0 2.0 35.0 90-91 22.477626667519242 29.5 2.0 34.0 2.0 35.0 92-93 21.63621124441496 29.0 2.0 34.0 2.0 35.0 94-95 20.673993493777527 28.5 2.0 34.0 2.0 35.0 96-97 19.437886956382734 26.5 2.0 33.5 2.0 35.0 98-99 17.949165940644708 24.5 2.0 33.0 2.0 34.5 100-101 14.524987211356315 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.723278409960983 1101 2 0.5933195877231299 1101 3 0.6514053290745601 1101 4 0.5260833526447755 1101 5 0.5358892435298728 1101 6 0.5327166051557057 1101 7 0.4794692065551729 1101 8 0.5040314283297676 1101 9 0.5288110379855553 1101 10-11 0.5724306864892057 1101 12-13 0.6453881629160847 1101 14-15 0.831820991796846 1101 16-17 0.7502975092188393 1101 18-19 0.7209119754157385 1101 20-21 0.7931467887304748 1101 22-23 0.812470094073646 1101 24-25 0.8159867822593014 1101 26-27 0.9596910886152301 1101 28-29 0.8653477829762934 1101 30-31 0.7848198233037635 1101 32-33 0.9107840538292749 1101 34-35 0.8426181285073469 1101 36-37 0.8663532018313091 1101 38-39 0.8061442856918362 1101 40-41 0.800705589872102 1101 42-43 0.7940364310977586 1101 44-45 0.8487115109193653 1101 46-47 1.075657976898185 1101 48-49 1.1431980465631284 1101 50-51 0.8519796112142686 1101 52-53 0.9517401974827848 1101 54-55 1.1189179964583857 1101 56-57 0.9844310920226533 1101 58-59 0.9814560977015105 1101 60-61 1.0188569346728542 1101 62-63 0.9902917244019243 1101 64-65 1.0487924040983394 1101 66-67 1.037383006985749 1101 68-69 0.7924815649097248 1101 70-71 0.9989716763346195 1101 72-73 0.9432943385657673 1101 74-75 1.0196898998186157 1101 76-77 1.2006208407829142 1101 78-79 0.8534140197374853 1101 80-81 1.0512078088981305 1101 82-83 1.1551269984211032 1101 84-85 1.2351137418943985 1101 86-87 1.3564678656008127 1101 88-89 1.305341785520973 1101 90-91 1.5373263470053153 1101 92-93 1.5308753779607578 1101 94-95 1.5184238745403853 1101 96-97 1.5292282832412063 1101 98-99 1.3992257653156166 1101 100-101 1.2514361500745057 1102 1 0.6124850482657962 1102 2 0.6018556475468664 1102 3 0.5874047086423531 1102 4 0.584072816490611 1102 5 0.5822894175067432 1102 6 0.5085203886728422 1102 7 0.5147791548178375 1102 8 0.6532066772422169 1102 9 0.6522845628799985 1102 10-11 0.6274685400496125 1102 12-13 0.6013876064459538 1102 14-15 0.5590723571874463 1102 16-17 0.5946506566730321 1102 18-19 0.8292464747245489 1102 20-21 0.4925002919737551 1102 22-23 0.6853177100069203 1102 24-25 0.5400825782714236 1102 26-27 0.5700546251107923 1102 28-29 0.3979574119882088 1102 30-31 0.45877750119230853 1102 32-33 0.5096001156678156 1102 34-35 0.4801882569352145 1102 36-37 0.5197663385238727 1102 38-39 0.6661762187619118 1102 40-41 0.6479839632523579 1102 42-43 0.8700124325241134 1102 44-45 0.7372870033674488 1102 46-47 0.6990860887135 1102 48-49 0.49088153978834015 1102 50-51 0.7748127157498956 1102 52-53 0.8180948624373983 1102 54-55 0.8068888873142868 1102 56-57 0.6865886130441865 1102 58-59 0.8901277571417907 1102 60-61 0.8688659293408136 1102 62-63 0.5627997816256034 1102 64-65 0.619855557860788 1102 66-67 0.7455276188598283 1102 68-69 0.5024596499009419 1102 70-71 0.5593870620939434 1102 72-73 0.5549756538820496 1102 74-75 0.7393602392414031 1102 76-77 0.7375063350619051 1102 78-79 0.5790116454649592 1102 80-81 0.7691788356734541 1102 82-83 0.7588263254600349 1102 84-85 0.5060929454225445 1102 86-87 0.6441462898309283 1102 88-89 0.9270613901393574 1102 90-91 0.8097657249420749 1102 92-93 0.7248148106650554 1102 94-95 0.6713121223870608 1102 96-97 0.9330174770071444 1102 98-99 0.9941164741034463 1102 100-101 0.7540326108925317 1103 1 0.6080385535484929 1103 2 0.5919152263484264 1103 3 0.5901034113750327 1103 4 0.6098902453572634 1103 5 0.5785358224486146 1103 6 0.6786463726898333 1103 7 0.5448952660249731 1103 8 0.4321799236682935 1103 9 0.5678922442742333 1103 10-11 0.6088632982590312 1103 12-13 0.6294390072285623 1103 14-15 0.8552453843973566 1103 16-17 0.7271327451080367 1103 18-19 0.7338915602661089 1103 20-21 0.6783933958036314 1103 22-23 0.7649922336811485 1103 24-25 0.8964651353540489 1103 26-27 0.9080374167037348 1103 28-29 0.7957328745741208 1103 30-31 0.829676523734399 1103 32-33 0.9689352366346071 1103 34-35 0.9975816237498876 1103 36-37 1.051653478256462 1103 38-39 0.7435619820208856 1103 40-41 1.053809294097178 1103 42-43 0.934770029551828 1103 44-45 0.9469931318457796 1103 46-47 1.0628191641186362 1103 48-49 0.8535971033902499 1103 50-51 1.0352141374842532 1103 52-53 0.9864644281954114 1103 54-55 1.0892624401207627 1103 56-57 1.1334164133349063 1103 58-59 0.8386721860295836 1103 60-61 1.1699427992373188 1103 62-63 1.1685506778255679 1103 64-65 1.0675029319296208 1103 66-67 1.2685183497337498 1103 68-69 1.23471190640997 1103 70-71 1.3091629343890219 1103 72-73 1.099877432479932 1103 74-75 1.0867358778965617 1103 76-77 0.9962694640960486 1103 78-79 1.0505377903813908 1103 80-81 0.8759808939180616 1103 82-83 1.1157608086731514 1103 84-85 1.2021805958305762 1103 86-87 1.222522520357309 1103 88-89 1.2794889947146686 1103 90-91 1.3313654496473504 1103 92-93 1.5104458311828388 1103 94-95 1.4104087447421385 1103 96-97 1.3302482408816125 1103 98-99 1.3186246365795569 1103 100-101 1.0124179712811774 1104 1 0.5003594852556965 1104 2 0.5430106053930928 1104 3 0.5273517768600477 1104 4 0.5398663810100714 1104 5 0.33733974063289196 1104 6 0.4365064893001289 1104 7 0.45735644591159286 1104 8 0.3889280131127748 1104 9 0.3281700108308456 1104 10-11 0.41106020758430617 1104 12-13 0.42869296329956086 1104 14-15 0.5791375957697369 1104 16-17 0.5826277421028152 1104 18-19 0.5728640620992351 1104 20-21 0.5741857974052849 1104 22-23 0.7401552414335484 1104 24-25 0.7059938741731813 1104 26-27 0.8552799339241375 1104 28-29 0.794991589437096 1104 30-31 0.7901718175798536 1104 32-33 0.6246739725280719 1104 34-35 0.7256740428565465 1104 36-37 0.6435026774940766 1104 38-39 0.6638433413889402 1104 40-41 0.5302417975951244 1104 42-43 0.6629846317902661 1104 44-45 0.8080709875188106 1104 46-47 0.642980862055623 1104 48-49 0.6096759628470565 1104 50-51 0.5681304415034276 1104 52-53 0.6429265463750298 1104 54-55 0.7741092223204973 1104 56-57 0.8562773745925156 1104 58-59 0.6868499665412457 1104 60-61 0.7686572827354823 1104 62-63 0.7537695485933078 1104 64-65 0.7357462735992506 1104 66-67 0.7380849569078656 1104 68-69 0.8933525873181267 1104 70-71 0.7824120967804262 1104 72-73 0.7682715712395769 1104 74-75 0.5516284638516709 1104 76-77 0.6863104447927952 1104 78-79 0.7904543539153934 1104 80-81 0.7503581960410663 1104 82-83 0.7953284685961037 1104 84-85 0.8502406918755483 1104 86-87 0.8983236958860417 1104 88-89 0.9628564199725602 1104 90-91 1.1508305002221384 1104 92-93 1.214566179960979 1104 94-95 0.9807954861050057 1104 96-97 0.897670683283394 1104 98-99 0.9934924921179338 1104 100-101 0.7698995267543065 1105 1 0.4532315978030077 1105 2 0.5549320668283073 1105 3 0.4069745020856246 1105 4 0.5542854477506367 1105 5 0.4971999839531591 1105 6 0.5256214799031298 1105 7 0.5269368123091454 1105 8 0.4688319433798611 1105 9 0.5893689830598134 1105 10-11 0.5832106693467196 1105 12-13 0.6379874205923812 1105 14-15 0.7287112993143268 1105 16-17 0.709940542274353 1105 18-19 0.6611642258510884 1105 20-21 0.7679713885776707 1105 22-23 0.7530966090694378 1105 24-25 0.8143384668376612 1105 26-27 0.8131903652956396 1105 28-29 0.7116596287405059 1105 30-31 0.8814184759513424 1105 32-33 0.929614797045005 1105 34-35 0.769845196561441 1105 36-37 0.8669818887250642 1105 38-39 0.7806989496488015 1105 40-41 0.8365128184139436 1105 42-43 0.7694117622152348 1105 44-45 0.698835813294103 1105 46-47 0.8802286586026611 1105 48-49 0.7711720923824217 1105 50-51 0.7273999316467794 1105 52-53 0.8013273429495271 1105 54-55 0.8102358343339588 1105 56-57 0.887692429523959 1105 58-59 0.8376702468410926 1105 60-61 1.0876370649091598 1105 62-63 1.0184887776017923 1105 64-65 0.9497860474183959 1105 66-67 0.9880704514763039 1105 68-69 0.8373145808615412 1105 70-71 0.9312166043711088 1105 72-73 0.9907572902526702 1105 74-75 1.0589853516315664 1105 76-77 1.0153583320080557 1105 78-79 1.143010240635025 1105 80-81 1.101185895998995 1105 82-83 1.3701088281675737 1105 84-85 1.2662436330280116 1105 86-87 1.2847025391761804 1105 88-89 1.4907326689792555 1105 90-91 1.1714871993201328 1105 92-93 1.314068766082805 1105 94-95 1.2876697993229023 1105 96-97 1.4500641536874923 1105 98-99 1.4962921517040222 1105 100-101 1.1837873459758228 1106 1 0.3159876173503342 1106 2 0.3349044016143239 1106 3 0.22707897408696454 1106 4 0.41659657875273837 1106 5 0.3860723790768148 1106 6 0.05202994282944928 1106 7 0.2171847874532915 1106 8 0.2983360891000615 1106 9 0.4039198812191813 1106 10-11 0.22306933008744778 1106 12-13 0.16909933915946596 1106 14-15 0.48513282677977543 1106 16-17 0.2573860888469568 1106 18-19 -0.045360215324144804 1106 20-21 0.3603661134284337 1106 22-23 0.24756615111635227 1106 24-25 0.348355823694547 1106 26-27 0.17551360813728678 1106 28-29 0.11369870123946413 1106 30-31 0.29145579371062524 1106 32-33 0.4356560939866867 1106 34-35 0.14639217018939377 1106 36-37 0.04505761283213161 1106 38-39 0.14840280335550204 1106 40-41 0.16227595662110872 1106 42-43 -0.1744888557101305 1106 44-45 0.3132365689547285 1106 46-47 -0.051283984606925515 1106 48-49 0.4926093098497617 1106 50-51 0.07911696953394909 1106 52-53 0.2502187034414405 1106 54-55 0.1415103026500546 1106 56-57 -0.283699405471431 1106 58-59 -0.7371225040752378 1106 60-61 0.1308941305797049 1106 62-63 0.2097359795083733 1106 64-65 0.15011646430938796 1106 66-67 -0.25691875495449423 1106 68-69 -0.40872983266325136 1106 70-71 -0.27913894458694344 1106 72-73 0.23080132122057861 1106 74-75 0.0446440473784655 1106 76-77 0.35198630725559354 1106 78-79 0.6085227376162976 1106 80-81 0.44600346880111985 1106 82-83 0.3974264914392336 1106 84-85 0.2689470842376096 1106 86-87 0.6265216193115108 1106 88-89 0.25891420182117386 1106 90-91 0.2584335903981376 1106 92-93 0.2558175384666299 1106 94-95 0.16912989635055453 1106 96-97 0.3626755327607434 1106 98-99 0.4183959634624017 1106 100-101 0.1590947023674385 1107 1 0.4525540406020383 1107 2 0.3778779007497981 1107 3 0.37250278140116677 1107 4 0.44589796830049977 1107 5 0.4030470516504252 1107 6 0.35127697229415844 1107 7 0.45352023867827285 1107 8 0.5103870740363305 1107 9 0.4866407887240882 1107 10-11 0.47479125117330767 1107 12-13 0.45240882352931777 1107 14-15 0.5084587980417723 1107 16-17 0.6353614846663049 1107 18-19 0.6049396681097292 1107 20-21 0.5815710823081233 1107 22-23 0.6139471486770205 1107 24-25 0.6383324327003024 1107 26-27 0.5206392169987311 1107 28-29 0.6091829518505989 1107 30-31 0.6892068191728455 1107 32-33 0.6851367741660397 1107 34-35 0.7145697464607039 1107 36-37 0.6688942197813361 1107 38-39 0.7607724657457737 1107 40-41 0.6261380802330478 1107 42-43 0.6520170637581373 1107 44-45 0.9024109541310423 1107 46-47 0.5962052207127755 1107 48-49 0.6427208210358728 1107 50-51 0.442482066921535 1107 52-53 0.47810354232700547 1107 54-55 0.5457394324307572 1107 56-57 0.5929947566306666 1107 58-59 0.46329800672462795 1107 60-61 0.4695420671691082 1107 62-63 0.5705478984599175 1107 64-65 0.4489411683791822 1107 66-67 0.6457321091815906 1107 68-69 0.6606252861797621 1107 70-71 0.6445534452606587 1107 72-73 0.6204931684178483 1107 74-75 0.7956727499516454 1107 76-77 1.0028389941091973 1107 78-79 1.0111167876455447 1107 80-81 0.9331412302648268 1107 82-83 0.9169366285448497 1107 84-85 0.8818010706869046 1107 86-87 0.7593560254161531 1107 88-89 0.6863161424009618 1107 90-91 0.8652650654997309 1107 92-93 1.0351284330360677 1107 94-95 0.9887580685435289 1107 96-97 0.8322730728611347 1107 98-99 0.9068153906897969 1107 100-101 0.6214954288967345 1108 1 0.371933203443362 1108 2 0.40568612585031616 1108 3 0.47483220645681357 1108 4 0.39186138799684755 1108 5 0.36703740571251586 1108 6 0.4183996847535951 1108 7 0.34683875384358487 1108 8 0.26345010296142846 1108 9 0.35176536560520333 1108 10-11 0.4128626683004981 1108 12-13 0.37777565372142163 1108 14-15 0.5438509496685953 1108 16-17 0.43098761019291487 1108 18-19 0.5369563772613546 1108 20-21 0.5035495705328614 1108 22-23 0.5610022920987419 1108 24-25 0.41761618733698214 1108 26-27 0.5978361078961498 1108 28-29 0.3714797848486526 1108 30-31 0.5992883162712488 1108 32-33 0.44645710288974527 1108 34-35 0.5697384726662307 1108 36-37 0.5920246905859514 1108 38-39 0.5030348190935214 1108 40-41 0.5971503216109539 1108 42-43 0.5659286362556841 1108 44-45 0.5703284335504932 1108 46-47 0.5188806771188581 1108 48-49 0.666758664444238 1108 50-51 0.5771785502063054 1108 52-53 0.5280257780279953 1108 54-55 0.5377976968796716 1108 56-57 0.5854742679136891 1108 58-59 0.6558024410133712 1108 60-61 0.5467150805837377 1108 62-63 0.7540480492130222 1108 64-65 0.7353002167724796 1108 66-67 0.8197760703118746 1108 68-69 0.7267280486491074 1108 70-71 0.6592262290841013 1108 72-73 0.6596240534756035 1108 74-75 0.633315827643326 1108 76-77 0.5678298994727022 1108 78-79 0.6255781639097435 1108 80-81 0.7105573842720467 1108 82-83 0.8626699625563603 1108 84-85 0.8769210218355532 1108 86-87 1.0343561031239403 1108 88-89 1.1625091617552883 1108 90-91 1.1539324589461124 1108 92-93 0.944880237198884 1108 94-95 1.0174275078239354 1108 96-97 0.9997184421694989 1108 98-99 0.6503592160433875 1108 100-101 0.3205131177315934 1109 1 0.6248256763611444 1109 2 0.5880782354806442 1109 3 0.5479149288593703 1109 4 0.5597804667036499 1109 5 0.5513647834913371 1109 6 0.5644377656224293 1109 7 0.4876939442084236 1109 8 0.5319328065912359 1109 9 0.5531895346029216 1109 10-11 0.541804590029507 1109 12-13 0.48763905827005516 1109 14-15 0.45906267562058645 1109 16-17 0.6701160869044358 1109 18-19 0.7083170475938729 1109 20-21 0.6176306808176975 1109 22-23 0.6392744628258313 1109 24-25 0.5368619368421435 1109 26-27 0.5055366800225372 1109 28-29 0.45735760522504876 1109 30-31 0.46352689325895113 1109 32-33 0.48373167583341115 1109 34-35 0.6493839932323411 1109 36-37 0.5941384420669991 1109 38-39 0.5069981920065985 1109 40-41 0.5618923969983882 1109 42-43 0.636406086676196 1109 44-45 0.5278412623856852 1109 46-47 0.6491961857165478 1109 48-49 0.6027193345871282 1109 50-51 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14 19807.0 15 23315.0 16 26656.0 17 30114.0 18 29587.0 19 28877.0 20 30609.0 21 33101.0 22 36905.0 23 41132.0 24 46142.0 25 51581.0 26 58219.0 27 66195.0 28 75700.0 29 86692.0 30 100966.0 31 118144.0 32 139009.0 33 168721.0 34 206909.0 35 254728.0 36 301224.0 37 292070.0 38 133002.0 39 7357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92685090871819 17.796478516838672 14.241442930447985 24.035227643995157 2 15.86843741440975 19.215798385855344 39.416117056475066 25.499647143259836 3 19.43921115804187 22.930137492383302 28.904586971089337 28.72606437848549 4 13.503023294683464 15.644080524160367 35.37738602766829 35.47551015348788 5 14.644300266917258 36.545670842038966 33.35541981985585 15.454609071187933 6 34.090652795139846 34.500144361773 16.70801571743802 14.70118712564913 7 30.291560073672603 31.600500052571885 19.564656821531845 18.54328305222367 8 27.399333091601356 34.193987110382096 19.02671831255624 19.3799614854603 9 26.885218505194665 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23.7410604098682 34-35 23.539485237317656 25.975855605437108 27.350405991757015 23.13425316548822 36-37 23.65034438833138 25.48275739364384 27.403331572775354 23.463566645249433 38-39 23.05534896336246 25.791207014305193 27.510704762422534 23.642739259909813 40-41 23.39909226530733 25.681160787524206 27.614074444797993 23.305672502370474 42-43 23.24018358001006 25.62705148942404 27.43013368770732 23.702631242858573 44-45 23.137070509379843 25.962733217138577 27.51631515821977 23.38388111526181 46-47 23.462439069818377 26.229150441766926 26.940378102365703 23.368032386048995 48-49 23.400545453564405 26.09114194522722 26.928867011770198 23.579445589438176 50-51 23.50871660579551 26.330972028044698 26.868580650885733 23.291730715274063 52-53 23.700607954366053 26.117377438901805 26.844496616103058 23.337517990629088 54-55 23.195292003536434 26.298903737715612 27.008268730545222 23.497535528202736 56-57 23.26102231160792 26.404347534148464 26.977025726454183 23.35760442778943 58-59 23.279231835470053 25.8682978651971 26.95960053781749 23.892869761515353 60-61 22.882116525533387 26.196651850886678 27.357146896849837 23.564084726730094 62-63 23.01059480077622 26.330714690941516 27.241101408463653 23.417589099818613 64-65 22.79483426173407 26.273064721604676 27.11704843504109 23.815052581620165 66-67 22.799987370719734 26.232074215405966 27.138185108479924 23.82975330539438 68-69 23.18627098321343 25.800499600319743 27.35257793764988 23.660651478816945 70-71 22.967406674561417 26.109477197663555 26.997506658072385 23.925609469702646 72-73 23.09819447267486 26.16786473544655 26.808820040655796 23.9251207512228 74-75 22.91917910778877 26.5318179474337 26.803591293499622 23.745411651277905 76-77 23.08077704400725 26.114130461935698 26.919772534466063 23.885319959590994 78-79 23.20001950358968 26.226213264159536 26.95158293811587 23.622184294134914 80-81 23.225744825072418 26.421306569296387 26.697078891667044 23.65586971396415 82-83 23.080247992925905 26.419399801166026 26.730639915670416 23.769712290237653 84-85 23.084601253388907 26.281580266788275 26.808818125120276 23.825000354702546 86-87 23.20443402174445 26.24358142978616 26.600953030904062 23.951031517565326 88-89 23.10286303763782 26.3311834783038 26.49728471450875 24.068668769549625 90-91 23.050663216770747 26.465444504257036 26.50086563896069 23.98302664001153 92-93 23.27335943188384 25.844840139882475 26.76928205801601 24.11251837021767 94-95 22.915209178676367 25.783565531991442 26.959803314077835 24.341421975254352 96-97 23.17315843644226 25.519142690205065 26.936911707413508 24.370787165939163 98-99 23.04381481468897 25.754332204967064 26.50969870681911 24.692154273524856 100-101 23.484986715323792 25.16317743845834 26.79153061877781 24.56030522744006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 417.0 1 291.0 2 208.5 3 277.5 4 257.0 5 272.5 6 509.5 7 1060.0 8 1686.0 9 1975.5 10 1763.5 11 1489.0 12 1538.5 13 1659.0 14 1798.0 15 1989.5 16 2119.0 17 2261.5 18 2478.0 19 2712.5 20 3020.5 21 3566.0 22 4266.5 23 5146.5 24 6298.5 25 7881.0 26 9974.5 27 12746.5 28 15484.0 29 18380.0 30 21864.5 31 26872.0 32 34478.5 33 37852.0 34 38047.5 35 40977.0 36 45704.5 37 49984.5 38 53511.0 39 56826.0 40 60204.0 41 66793.5 42 72182.5 43 76098.0 44 81289.5 45 88218.0 46 92735.5 47 94460.0 48 95737.0 49 95021.0 50 95443.5 51 94807.0 52 94246.5 53 95976.0 54 97777.0 55 94173.0 56 86798.0 57 80977.5 58 76346.0 59 68409.5 60 56020.0 61 46319.0 62 39547.0 63 31236.0 64 23886.0 65 18886.0 66 14243.5 67 10980.5 68 8513.0 69 6314.5 70 4832.0 71 3644.5 72 2800.0 73 2232.0 74 1695.5 75 1323.5 76 948.0 77 626.5 78 390.0 79 217.5 80 140.5 81 96.0 82 60.0 83 40.0 84 31.0 85 19.5 86 10.5 87 9.5 88 8.5 89 5.5 90 4.5 91 4.0 92 3.0 93 1.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18527547537786446 2 0.0481572363740998 3 0.045399685079649275 4 0.05219365203699115 5 0.057748719137405986 6 0.06226470893846265 7 0.03528866366666401 8 0.03305065102189256 9 0.039884582490748215 10-11 0.040364156628913524 12-13 0.03738680052113723 14-15 0.03390988801943874 16-17 0.03167187537466729 18-19 0.03910527451622959 20-21 0.04885661532559088 22-23 0.045299773800864834 24-25 0.06636107136862467 26-27 0.05051514255341257 28-29 0.043161672434877826 30-31 0.0514543085739863 32-33 0.0478175380262327 34-35 0.04731798163231051 36-37 0.044720288383915084 38-39 0.025837056693656033 40-41 0.024298423000375668 42-43 0.02124113786957182 44-45 0.003956486639863801 46-47 0.011130116456586552 48-49 0.010610577806907467 50-51 0.008552405463948014 52-53 0.008912086067571996 54-55 0.010550631039636802 56-57 0.008152760348810257 58-59 0.010230914947526596 60-61 0.010810400364476346 62-63 0.0038565753610793614 64-65 0.006294410563419684 66-67 0.0039165221283500255 68-69 0.008792192533030669 70-71 0.02228021516892999 72-73 0.019402770339938136 74-75 0.02010214929142921 76-77 0.012089264732917169 78-79 0.004655865591354877 80-81 0.006234463796149019 82-83 0.005295297775575289 84-85 0.004935617171951307 86-87 0.008092813581539593 88-89 0.029313969195354523 90-91 0.03550846847998977 92-93 0.02192053456530601 94-95 0.03077267386560734 96-97 0.03438946215760405 98-99 0.020801528242920288 100-101 0.12388998569270487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2502220.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.51322437367097 #Duplication Level Percentage of deduplicated Percentage of total 1 75.918112405629 39.10786758376741 2 12.809393816115483 13.197063554805407 3 4.584991330872887 7.085630615357739 4 2.1352570891529807 4.399759101160362 5 1.1663911356205396 3.004228413834088 6 0.7221550654268867 2.2320321550750957 7 0.4899190974494572 1.766611867430219 8 0.3512000457312672 1.447315740463862 9 0.26947159346080213 1.2493215590649256 >10 1.383946306025166 12.81735253950578 >50 0.10202309351197271 3.643218398597921 >100 0.060354597711948246 5.787417404099053 >500 0.003509184461160596 1.22806700591331 >1k 0.003197256953497424 2.7866206579147317 >5k 7.798187691457132E-5 0.2474934030101726 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6161 0.2462213554363725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4761 0.19027103931708644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3370 0.13468040380142432 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3093 0.12361023411210845 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3070 0.12269105034729161 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2903 0.11601697692449106 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2734 0.10926297447866294 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 3.9964511513775766E-5 0.0 0.0 0.0 2 0.0 3.9964511513775766E-5 0.0 0.0 0.0 3 0.0 3.9964511513775766E-5 0.0 0.0 0.0 4 0.0 3.9964511513775766E-5 0.0 0.0 0.0 5 0.0 3.9964511513775766E-5 0.0 0.0 0.0 6 0.0 3.9964511513775766E-5 0.0 0.0 0.0 7 0.0 3.9964511513775766E-5 0.0 0.0 0.0 8 0.0 3.9964511513775766E-5 0.0 0.0 0.0 9 0.0 3.9964511513775766E-5 0.0 3.9964511513775766E-5 0.0 10-11 0.0 3.9964511513775766E-5 0.0 3.9964511513775766E-5 0.0 12-13 3.9964511513775766E-5 3.9964511513775766E-5 0.0 3.9964511513775766E-5 0.0 14-15 1.198935345413273E-4 3.9964511513775766E-5 0.0 3.9964511513775766E-5 0.0 16-17 1.398757902982152E-4 3.9964511513775766E-5 0.0 3.9964511513775766E-5 0.0 18-19 1.9982255756887882E-4 3.9964511513775766E-5 0.0 3.9964511513775766E-5 0.0 20-21 2.397870690826546E-4 3.9964511513775766E-5 0.0 7.992902302755153E-5 0.0 22-23 2.397870690826546E-4 3.9964511513775766E-5 0.0 1.7984030181199094E-4 0.0 24-25 2.797515805964304E-4 5.994676727066365E-5 0.0 3.39698347867094E-4 0.0 26-27 2.797515805964304E-4 7.992902302755153E-5 0.0 9.791305320875064E-4 0.0 28-29 3.39698347867094E-4 7.992902302755153E-5 0.0 0.0031172318980745095 0.0 30-31 3.596806036239819E-4 7.992902302755153E-5 0.0 0.010790418108719457 0.0 32-33 3.9964511513775763E-4 7.992902302755153E-5 0.0 0.028055087082670585 0.0 34-35 4.995563939221971E-4 7.992902302755153E-5 0.0 0.05419187761267994 0.0 36-37 5.19538649679085E-4 7.992902302755153E-5 0.0 0.0974534613263422 0.0 38-39 5.994676727066365E-4 7.992902302755153E-5 0.0 0.16381453269496687 0.0 40-41 6.194499284635244E-4 7.992902302755153E-5 0.0 0.23605038725611655 0.0 42-43 6.993789514910759E-4 9.991127878443941E-5 0.0 0.31176315431896473 1.9982255756887883E-5 44-45 7.593257187617396E-4 1.398757902982152E-4 0.0 0.40793775127686616 3.9964511513775766E-5 46-47 9.391660205737305E-4 1.5985804605510306E-4 0.0 0.5202180463748192 3.9964511513775766E-5 48-49 9.591482763306184E-4 1.7984030181199094E-4 0.0 0.6400516341488758 3.9964511513775766E-5 50-51 9.591482763306184E-4 1.9982255756887884E-4 0.0 0.7709553916122484 3.9964511513775766E-5 52-53 9.591482763306184E-4 1.9982255756887884E-4 0.0 0.8946255724916274 3.9964511513775766E-5 54-55 9.591482763306184E-4 2.797515805964304E-4 0.0 1.0352606885086044 3.9964511513775766E-5 56-57 9.591482763306184E-4 3.197160921102061E-4 0.0 1.2010534645235031 3.9964511513775766E-5 58-59 9.591482763306184E-4 3.596806036239819E-4 0.0 1.3817530033330403 3.9964511513775766E-5 60-61 9.591482763306184E-4 3.596806036239819E-4 0.0 1.576779819520266 3.9964511513775766E-5 62-63 9.591482763306184E-4 3.596806036239819E-4 0.0 1.774264453165589 3.9964511513775766E-5 64-65 9.591482763306184E-4 3.596806036239819E-4 0.0 1.9795221843003412 3.9964511513775766E-5 66-67 0.0011190063223857215 3.596806036239819E-4 0.0 2.21575241185827 3.9964511513775766E-5 68-69 0.001198935345413273 3.596806036239819E-4 0.0 2.4763609914396016 3.9964511513775766E-5 70-71 0.0012189176011701609 3.9964511513775763E-4 0.0 2.758170744378991 3.9964511513775766E-5 72-73 0.0012788643684408245 4.3960962665153344E-4 0.0 3.0463748191605857 3.9964511513775766E-5 74-75 0.0013188288799546003 4.3960962665153344E-4 0.0 3.336377296960299 7.992902302755153E-5 76-77 0.0013188288799546003 4.3960962665153344E-4 0.0 3.661668438426677 7.992902302755153E-5 78-79 0.0013188288799546003 4.3960962665153344E-4 0.0 4.01807195210653 7.992902302755153E-5 80-81 0.0014786869260097035 4.3960962665153344E-4 0.0 4.394817402146893 9.991127878443941E-5 82-83 0.0015186514375234792 4.3960962665153344E-4 0.0 4.791545107944145 1.198935345413273E-4 84-85 0.0015386336932803671 4.3960962665153344E-4 0.0 5.188852299158347 1.198935345413273E-4 86-87 0.0015785982047941429 4.3960962665153344E-4 0.0 5.59956758398542 1.198935345413273E-4 88-89 0.0015985804605510307 4.3960962665153344E-4 0.0 6.0444125616452595 1.198935345413273E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3290 0.0 51.529675 1 GTATCAA 6020 0.0 48.412586 1 ATCAACG 8040 0.0 35.76651 3 TATCAAC 8080 0.0 35.471794 2 TCAACGC 8095 0.0 35.406063 4 CAACGCA 8355 0.0 34.243946 5 AACGCAG 8830 0.0 32.44981 6 GTGGTAT 1585 0.0 29.444225 1 ACGCAGA 10365 0.0 27.361992 7 GTACATG 8690 0.0 27.290588 1 CGCAGAG 10360 0.0 27.28349 8 TACATGG 8695 0.0 26.621748 2 TAAGGTG 1330 0.0 25.728508 5 ACATGGG 9075 0.0 24.773748 3 TGGTATC 1710 0.0 24.73837 2 GCAGAGT 11630 0.0 24.099405 9 CATGGGG 6180 0.0 22.996403 4 GAGTACT 6480 0.0 21.331297 12-13 AGAGTAC 10935 0.0 20.221493 10-11 CAGAGTA 10865 0.0 20.155033 10-11 >>END_MODULE