FastQCFastQC Report
Thu 26 May 2016
SRR938602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502220
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62000.24777997138540975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48430.19354812926121603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34670.1385569614182606No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31020.12396991471573242No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30930.12361023411210845No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28410.11353917721063697No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25810.10314840421705525No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25220.10079049803774248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.049.8127861
GTATCAA63300.042.8264921
ATCAACG81800.033.0099833
TATCAAC83800.032.178942
TCAACGC83700.032.1457674
CAACGCA87300.030.8201735
AACGCAG90650.029.6551066
GTGGTAT17500.028.5359691
TAAGGTG14450.028.2919885
ACGCAGA105300.025.3037787
CGCAGAG106350.025.0539518
TGGTATC20100.024.607642
GTACATG85700.024.1961941
TACATGG86250.023.1591632
AGGTAAG15300.022.691552
ACATGGG86400.022.393963
GGTAAGG16100.022.1455063
TAAGGTA8400.022.0708074
GCAGAGT122200.021.3767839
GAGTACT69050.020.94471512-13
Produced by FastQC (version 0.11.5)