##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938602_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2502220 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.785142393554523 31.0 31.0 34.0 30.0 34.0 2 32.017133585376186 31.0 31.0 34.0 30.0 34.0 3 32.08594488094572 33.0 31.0 34.0 30.0 34.0 4 35.761188464643396 37.0 35.0 37.0 35.0 37.0 5 35.61896715716444 37.0 35.0 37.0 33.0 37.0 6 35.6464147836721 37.0 35.0 37.0 33.0 37.0 7 35.5830006953825 37.0 35.0 37.0 33.0 37.0 8 35.56740614334471 37.0 35.0 37.0 33.0 37.0 9 37.14437699323001 39.0 37.0 39.0 33.0 39.0 10-11 37.05483190926458 39.0 37.0 39.0 33.0 39.0 12-13 36.94637461933803 39.0 37.0 39.0 33.0 39.0 14-15 38.00888670860276 40.0 37.0 41.0 33.0 41.0 16-17 37.93461386288975 40.0 37.0 41.0 33.0 41.0 18-19 38.02634580492523 40.0 37.0 41.0 33.0 41.0 20-21 38.06425674001487 40.0 37.0 41.0 33.0 41.0 22-23 37.9708049252264 40.0 37.0 41.0 33.0 41.0 24-25 37.87080752291965 40.0 37.0 41.0 32.0 41.0 26-27 37.680246740894084 40.0 37.0 41.0 32.0 41.0 28-29 37.55752651645339 39.5 36.5 41.0 32.0 41.0 30-31 37.32130348250753 39.0 36.0 41.0 31.0 41.0 32-33 37.115410715284824 39.0 36.0 41.0 31.0 41.0 34-35 36.97244766647218 39.0 36.0 40.5 30.5 41.0 36-37 36.98321810232514 39.0 36.0 41.0 31.0 41.0 38-39 36.8619565825547 39.0 35.0 41.0 30.0 41.0 40-41 36.68992154966389 39.0 35.0 41.0 30.0 41.0 42-43 36.47448485744658 39.0 35.0 40.0 30.0 41.0 44-45 36.22323077107528 39.0 35.0 40.0 30.0 41.0 46-47 35.873546091071134 38.0 35.0 40.0 28.5 41.0 48-49 35.52748199598756 38.0 34.5 40.0 27.5 41.0 50-51 35.157637417972836 38.0 34.0 40.0 26.5 41.0 52-53 34.648649399333394 38.0 33.0 40.0 26.0 41.0 54-55 34.31784515350369 37.5 33.0 40.0 24.5 41.0 56-57 33.973997690051235 37.0 33.0 40.0 24.0 41.0 58-59 33.51794366602457 36.5 32.0 39.0 23.0 41.0 60-61 33.13392567400149 36.0 32.0 39.0 22.5 40.0 62-63 32.66156752803511 35.5 31.0 39.0 21.5 40.0 64-65 32.149646114250544 35.0 31.0 38.0 20.0 40.0 66-67 31.643228413169105 35.0 30.0 38.0 18.5 40.0 68-69 31.099147556969413 34.0 29.5 37.5 17.0 39.5 70-71 30.51732701361191 34.0 29.0 36.5 13.0 39.0 72-73 30.07742724460679 34.0 29.0 36.0 9.5 39.0 74-75 29.56355436372501 34.0 28.0 36.0 7.5 38.5 76-77 28.326423735722678 31.5 26.5 34.5 7.0 36.5 78-79 29.02888954608308 33.0 28.0 35.0 6.5 37.0 80-81 28.767459695790137 33.0 28.0 35.0 2.0 36.5 82-83 28.30617951259282 33.0 27.0 35.0 2.0 36.0 84-85 27.79248507325495 33.0 26.0 35.0 2.0 36.0 86-87 27.229795541559096 32.5 25.5 35.0 2.0 35.0 88-89 26.593160073854417 32.0 25.0 34.0 2.0 35.0 90-91 26.051975845449242 32.0 24.0 34.0 2.0 35.0 92-93 25.311581515614137 31.0 21.0 34.0 2.0 35.0 94-95 24.540135559623053 31.0 18.5 34.0 2.0 35.0 96-97 23.537120836697014 31.0 4.5 34.0 2.0 35.0 98-99 22.283423919559432 30.0 2.0 34.0 2.0 35.0 100-101 18.63423839630408 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3594743896339274 1101 2 0.37009411035236894 1101 3 0.3237641274283263 1101 4 0.20769031612525168 1101 5 0.08272428706609247 1101 6 0.16523921958235377 1101 7 0.2730006996712717 1101 8 0.25690636538686107 1101 9 0.2487409393417508 1101 10-11 0.34862009058542043 1101 12-13 0.421629615901395 1101 14-15 0.5196561466393774 1101 16-17 0.38787025914021456 1101 18-19 0.5091543812155876 1101 20-21 0.5093808173262673 1101 22-23 0.5022261424620496 1101 24-25 0.549926259070098 1101 26-27 0.6093055211473271 1101 28-29 0.5271796617970708 1101 30-31 0.47655065511863626 1101 32-33 0.4339457003225178 1101 34-35 0.40948295102549537 1101 36-37 0.44823591866575896 1101 38-39 0.5355140271022023 1101 40-41 0.6037843556967672 1101 42-43 0.7312327584670797 1101 44-45 0.5759309099595455 1101 46-47 0.6008420737349383 1101 48-49 0.5289521231524716 1101 50-51 0.3920150106336706 1101 52-53 0.3657507119149699 1101 54-55 0.4123119580690755 1101 56-57 0.5121112971051716 1101 58-59 0.5657115634089394 1101 60-61 0.6335178121999974 1101 62-63 0.49244762935672526 1101 64-65 0.5679035180843641 1101 66-67 0.6072364358951532 1101 68-69 0.5891266163062419 1101 70-71 0.48893048931375915 1101 72-73 0.3474094757098882 1101 74-75 0.5122768535812909 1101 76-77 0.6297172367498547 1101 78-79 0.6105431728539479 1101 80-81 0.8358435933918216 1101 82-83 0.6503789430056344 1101 84-85 0.6343756709828376 1101 86-87 0.5834969208582486 1101 88-89 0.6116045760248277 1101 90-91 0.636837656842058 1101 92-93 0.7182994706048298 1101 94-95 0.8775174523288314 1101 96-97 0.8232332999737366 1101 98-99 0.697921408866744 1101 100-101 0.5871642703683371 1102 1 0.31301297196232625 1102 2 0.2980953512167801 1102 3 0.3341055170525564 1102 4 0.1664470851126012 1102 5 0.22659276183424737 1102 6 0.2925168893796979 1102 7 0.30107173915906316 1102 8 0.2628410872837179 1102 9 0.3197094292209499 1102 10-11 0.3759539670634453 1102 12-13 0.3703032421757442 1102 14-15 0.4407060682458521 1102 16-17 0.3395725700103327 1102 18-19 0.3512271204957429 1102 20-21 0.29489308565360517 1102 22-23 0.3063674474964202 1102 24-25 0.3821518757245528 1102 26-27 0.37938957532001183 1102 28-29 0.41060604663613276 1102 30-31 0.3510671217572181 1102 32-33 0.3239841655203506 1102 34-35 0.2710454967483926 1102 36-37 0.3189256138144785 1102 38-39 0.34332163875001953 1102 40-41 0.483970181539199 1102 42-43 0.39865910812699923 1102 44-45 0.4323289172208433 1102 46-47 0.2897415341348122 1102 48-49 0.467703311448048 1102 50-51 0.40801432623938183 1102 52-53 0.2807215172215862 1102 54-55 0.4474779116917702 1102 56-57 0.4051499837717145 1102 58-59 0.4986637093768138 1102 60-61 0.45108060097880553 1102 62-63 0.4394306578174607 1102 64-65 0.5417813233214233 1102 66-67 0.6019759543086352 1102 68-69 0.4504658544058948 1102 70-71 0.274009254621685 1102 72-73 -0.014979460128945021 1102 74-75 0.1970450829749062 1102 76-77 0.28128194918831895 1102 78-79 0.31074793147303836 1102 80-81 0.5453132597735895 1102 82-83 0.2185473327841727 1102 84-85 0.07909684027368158 1102 86-87 -0.015504311211383737 1102 88-89 0.22867549638324292 1102 90-91 0.1798787820630281 1102 92-93 0.25498676237826956 1102 94-95 0.20203379903850305 1102 96-97 0.06185080751526684 1102 98-99 0.15258652338685863 1102 100-101 -0.1154529781267577 1103 1 0.280714335628641 1103 2 0.2737705009564735 1103 3 0.28340509466042363 1103 4 0.1545882115827979 1103 5 0.11651326333319645 1103 6 0.13673383505064862 1103 7 0.23083210592027115 1103 8 0.27460157465733914 1103 9 0.3210734685679526 1103 10-11 0.37316426102201206 1103 12-13 0.3191647449317969 1103 14-15 0.5341390332346734 1103 16-17 0.5068720891771363 1103 18-19 0.5229038133282558 1103 20-21 0.5686932097476145 1103 22-23 0.5864470330373592 1103 24-25 0.5658075882143976 1103 26-27 0.5637582377283579 1103 28-29 0.5277105964596345 1103 30-31 0.5792497196242365 1103 32-33 0.5307843275388748 1103 34-35 0.5045839229926585 1103 36-37 0.48012791129442434 1103 38-39 0.5322853076801408 1103 40-41 0.6871162226543603 1103 42-43 0.7499466456318657 1103 44-45 0.7632167924404598 1103 46-47 0.8087339479870863 1103 48-49 0.6620983469416686 1103 50-51 0.6113067031618726 1103 52-53 0.8142065382790165 1103 54-55 0.7866976799418595 1103 56-57 0.8702040914947631 1103 58-59 0.7250031599561879 1103 60-61 0.7888457151440562 1103 62-63 0.5662389891509463 1103 64-65 0.6705925045894432 1103 66-67 0.7246947321691692 1103 68-69 0.5344689803812095 1103 70-71 0.5207658531775685 1103 72-73 0.5495128983908302 1103 74-75 0.6415472863274339 1103 76-77 0.5934281165113084 1103 78-79 0.6482216174505382 1103 80-81 0.5519354407693022 1103 82-83 0.6730084533107892 1103 84-85 0.5711598130090607 1103 86-87 0.5425460855039823 1103 88-89 0.4655885056132014 1103 90-91 0.6474370739177111 1103 92-93 0.5702747587076722 1103 94-95 0.8208208609325638 1103 96-97 0.5965911457771291 1103 98-99 0.5453132063341322 1103 100-101 0.07758369115525099 1104 1 0.3119938294278306 1104 2 0.27151902619000623 1104 3 0.2863953298029003 1104 4 0.16837340675141377 1104 5 0.09716487201911406 1104 6 0.19846684325596442 1104 7 0.19517666571824321 1104 8 0.1538058373108271 1104 9 0.23109159788223366 1104 10-11 0.2998917943213115 1104 12-13 0.21934831801031862 1104 14-15 0.3491888123410334 1104 16-17 0.3755200964827239 1104 18-19 0.5080436820284433 1104 20-21 0.46765808833261957 1104 22-23 0.34855869418933594 1104 24-25 0.48497086099019526 1104 26-27 0.44211479274244425 1104 28-29 0.4406465844338143 1104 30-31 0.4353345926027501 1104 32-33 0.4334880711627065 1104 34-35 0.3540342595662551 1104 36-37 0.4914669190664043 1104 38-39 0.4358809805151793 1104 40-41 0.45268015021544983 1104 42-43 0.46652569544034606 1104 44-45 0.3951670769886775 1104 46-47 0.2984375135005024 1104 48-49 0.39031394378293527 1104 50-51 0.5514063654253505 1104 52-53 0.6211481621318597 1104 54-55 0.676250402460532 1104 56-57 0.6643317115846443 1104 58-59 0.6465589880839246 1104 60-61 0.557253212184726 1104 62-63 0.6412400755632675 1104 64-65 0.5929624533842244 1104 66-67 0.46495894764482415 1104 68-69 0.5398034178766942 1104 70-71 0.4754103576450177 1104 72-73 0.12040864687688924 1104 74-75 0.32711745081331856 1104 76-77 0.21352594870559116 1104 78-79 0.23611436315416157 1104 80-81 0.4111440551926968 1104 82-83 0.2319209974252523 1104 84-85 0.29781319484228774 1104 86-87 0.41595387939534945 1104 88-89 0.24052817147561356 1104 90-91 0.16528307940914289 1104 92-93 0.3924278381816393 1104 94-95 0.6348007534297722 1104 96-97 0.43668204023416024 1104 98-99 0.39795139798093615 1104 100-101 0.3344345919287761 1105 1 0.22311953253317895 1105 2 0.26928081213138455 1105 3 0.15435375894256254 1105 4 0.1851010259092689 1105 5 -0.017152767995405327 1105 6 0.18970022043947665 1105 7 0.13547379334600151 1105 8 0.1812661402851461 1105 9 0.17293394350325997 1105 10-11 0.2502660789735245 1105 12-13 0.3215857901031285 1105 14-15 0.35149461948483207 1105 16-17 0.20259999358690095 1105 18-19 0.2359218601462416 1105 20-21 0.20775926279029733 1105 22-23 0.189739280505556 1105 24-25 0.3318386152303461 1105 26-27 0.238817348295278 1105 28-29 0.21071434817112333 1105 30-31 0.4893679558373023 1105 32-33 0.3279128658793198 1105 34-35 0.3969464456868934 1105 36-37 0.4303580956831965 1105 38-39 0.32247061629158225 1105 40-41 0.18323897660361155 1105 42-43 0.25717336481445585 1105 44-45 0.22285709257738517 1105 46-47 0.032436983736914726 1105 48-49 0.02242867247201019 1105 50-51 -0.07720880733025126 1105 52-53 0.06003125132134102 1105 54-55 0.1327995491627476 1105 56-57 0.04800927995493254 1105 58-59 0.019599066851014868 1105 60-61 0.03553884082638348 1105 62-63 0.06937237697055565 1105 64-65 0.08159694692950126 1105 66-67 0.12367537647435256 1105 68-69 0.13823245366986825 1105 70-71 0.029485641947765373 1105 72-73 0.21997103544290297 1105 74-75 0.043472677331770626 1105 76-77 0.05068574810771409 1105 78-79 0.19029099998638443 1105 80-81 0.2526094821828906 1105 82-83 0.2372680482224574 1105 84-85 0.2894247519985136 1105 86-87 0.2507163872694598 1105 88-89 0.08038160354975687 1105 90-91 0.07078868829967533 1105 92-93 0.22095694290343815 1105 94-95 0.03548269420457473 1105 96-97 0.0595881570744865 1105 98-99 -0.04511125880571498 1105 100-101 -0.18742142070244938 1106 1 0.16750728273682824 1106 2 0.15702414748089666 1106 3 0.20972007931401748 1106 4 0.14707797353368335 1106 5 0.08769883612173857 1106 6 0.1250479594766034 1106 7 0.20861940793692924 1106 8 0.17057419236900984 1106 9 0.09611785070884338 1106 10-11 0.13566200688444496 1106 12-13 0.16355774528636857 1106 14-15 0.2913464857789876 1106 16-17 0.08981506071529566 1106 18-19 0.3736879790475811 1106 20-21 0.31668915961072486 1106 22-23 0.34667061302207713 1106 24-25 0.2909791768208905 1106 26-27 0.36990164595998465 1106 28-29 0.4375233141818953 1106 30-31 0.32851187033953266 1106 32-33 0.2403028346174807 1106 34-35 0.2197981635371562 1106 36-37 -0.11621126269209725 1106 38-39 -0.015896958836904673 1106 40-41 0.2826280672334036 1106 42-43 0.3861098049823326 1106 44-45 0.3028017326083443 1106 46-47 0.014126393336432841 1106 48-49 0.17250743635538868 1106 50-51 0.36434019355375824 1106 52-53 0.2582276211667036 1106 54-55 0.0602282868653603 1106 56-57 0.3303661700776601 1106 58-59 0.4448452362971267 1106 60-61 0.10451392071652066 1106 62-63 0.18413198280402554 1106 64-65 -0.2705354384308336 1106 66-67 -0.04756853160269614 1106 68-69 -0.6959846008341479 1106 70-71 -0.4252129024897613 1106 72-73 -0.12225497296005727 1106 74-75 0.12389152542197834 1106 76-77 -0.07149494429070202 1106 78-79 0.05106082555373703 1106 80-81 -0.8118826661694207 1106 82-83 -5.321906171111834E-4 1106 84-85 0.02315696365733899 1106 86-87 0.265985872006695 1106 88-89 0.29194612088187455 1106 90-91 0.1150479348398612 1106 92-93 -0.081304721573499 1106 94-95 -0.02573268458212752 1106 96-97 0.015972905905215384 1106 98-99 -0.28316207978903307 1106 100-101 -0.3899341104392171 1107 1 0.15337633983003585 1107 2 0.09739957855523329 1107 3 0.11731182653574024 1107 4 0.01236243723011654 1107 5 0.04964764207499428 1107 6 0.1640846514525478 1107 7 0.07233122259246727 1107 8 0.15748448138215565 1107 9 0.1546890904142586 1107 10-11 0.15292291022441873 1107 12-13 0.20201398287043304 1107 14-15 0.28889030176868147 1107 16-17 0.2648599719427125 1107 18-19 0.18178137539056394 1107 20-21 0.2045569067391213 1107 22-23 0.21916938817041398 1107 24-25 0.2608324884136479 1107 26-27 0.28578609254737586 1107 28-29 0.3505072169956449 1107 30-31 0.32075210025736567 1107 32-33 0.4240752999522357 1107 34-35 0.3358292034815804 1107 36-37 0.334656848219403 1107 38-39 0.22616267566908732 1107 40-41 0.24781722148521368 1107 42-43 0.2210106027992893 1107 44-45 0.28893239382085056 1107 46-47 0.25380177956434835 1107 48-49 0.3137352086461789 1107 50-51 0.3427000674998908 1107 52-53 0.356555990286445 1107 54-55 0.43707776966255807 1107 56-57 0.40321244017753344 1107 58-59 0.1768729684940027 1107 60-61 0.27565637247638364 1107 62-63 0.26103408885760615 1107 64-65 0.28993658958651025 1107 66-67 0.3757831896271142 1107 68-69 0.4649589626676054 1107 70-71 0.29215376581868213 1107 72-73 0.2843754709330959 1107 74-75 0.39005395068874193 1107 76-77 0.23136075306249637 1107 78-79 0.2804258555420809 1107 80-81 0.45616024122660903 1107 82-83 0.3211039174048693 1107 84-85 0.5026999967788832 1107 86-87 0.4530669489774972 1107 88-89 0.296851576159451 1107 90-91 0.3877329652084036 1107 92-93 0.19003448413504387 1107 94-95 0.1478285791709908 1107 96-97 0.3857817599193112 1107 98-99 0.09574243189911513 1107 100-101 -0.2561852780447218 1108 1 0.2485009817031134 1108 2 0.24586256096868908 1108 3 0.2718772718903537 1108 4 0.19432072425667712 1108 5 0.09263445803102854 1108 6 0.12567878002191435 1108 7 0.11578428525404405 1108 8 0.15821479326729815 1108 9 0.20305964295051382 1108 10-11 0.16152381971633645 1108 12-13 0.19508435588757322 1108 14-15 0.31054618965924163 1108 16-17 0.29629813957620854 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-0.136635342354829 2316 68-69 -0.14767890685464735 2316 70-71 -0.15338130482882306 2316 72-73 -0.32830645533424274 2316 74-75 0.04849782505664635 2316 76-77 0.182368469793591 2316 78-79 0.18049377389339938 2316 80-81 -0.17815709507301491 2316 82-83 0.06200531249570318 2316 84-85 0.00953914116732335 2316 86-87 0.3307435440829991 2316 88-89 0.49995657136643246 2316 90-91 0.3295288304976616 2316 92-93 0.21297858647443135 2316 94-95 0.33730732578320755 2316 96-97 0.4825575749543134 2316 98-99 0.6033490211323596 2316 100-101 0.5077091548976611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 43.0 5 75.0 6 182.0 7 437.0 8 1059.0 9 1980.0 10 3038.0 11 4672.0 12 6641.0 13 9125.0 14 12085.0 15 15774.0 16 19463.0 17 23938.0 18 23234.0 19 21013.0 20 21209.0 21 22377.0 22 24791.0 23 27999.0 24 32515.0 25 38055.0 26 45655.0 27 55148.0 28 66011.0 29 79047.0 30 94017.0 31 111546.0 32 135197.0 33 166144.0 34 212080.0 35 275656.0 36 361961.0 37 396180.0 38 185787.0 39 8067.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54745016793195 18.680251490286164 14.888514285805634 23.88378405597625 2 16.316858063829976 19.967318144706972 38.486543024179134 25.229280767283917 3 19.535784138585356 23.185575242247015 29.397273916772377 27.881366702395255 4 14.005882776094827 16.355396407989705 35.031811751164966 34.6069090647505 5 14.540667442964697 36.72180356086386 33.55191549738609 15.185613498785349 6 34.06119366002989 34.97682058332201 16.37362022523999 14.58836553140811 7 30.224440696661365 31.760876341808476 19.559071544468512 18.45561141706165 8 27.31918056765592 34.67001302843075 18.86440840533606 19.146397998577264 9 26.603815811559333 16.626755441168243 19.21937319660142 37.550055550671004 10-11 25.78328444341425 25.951315232073917 26.605694143600484 21.65970618091135 12-13 26.4248747112564 22.963808138373125 27.111724788387914 23.49959236198256 14-15 22.965326789810646 24.704042809984735 25.02341920374707 27.307211196457548 16-17 23.08274252463812 27.977815699658702 25.87168594288272 23.067755832820456 18-19 23.242520641670197 26.34850252975358 27.82854824915475 22.580428579421476 20-21 23.449916474170937 25.55358841348882 28.172762586822902 22.82373252551734 22-23 23.39172814540688 25.28360815595751 28.21462541263358 23.11003828600203 24-25 23.371935836784445 25.67126093407335 28.05141449816912 22.90538873097309 26-27 22.72439046860221 26.14573597307896 27.84614686707566 23.283726691243167 28-29 22.921945312562446 26.397499020869468 27.849849333791592 22.830706332776497 30-31 22.978814812446547 25.900160657336286 27.80349050043561 23.31753402978155 32-33 22.74404328955887 25.837776054863284 27.78728489101678 23.630895764561068 34-35 23.485436512916767 26.05540016515171 27.469521675562426 22.9896416463691 36-37 23.68327766936281 25.564289587993365 27.513321186771574 23.239111555872253 38-39 23.000376882062294 25.884823562842385 27.622337795835435 23.49246175925989 40-41 23.319886470025526 25.680411483209603 27.751676061885544 23.248025984879323 42-43 23.221705944968214 25.72585378077007 27.505221886985037 23.54721838727668 44-45 23.15748078855592 25.965867502830985 27.618660153515705 23.257991555097384 46-47 23.457389837824014 26.313713422480838 26.98581659486376 23.243080144831392 48-49 23.368688604519186 26.11788731606334 27.03265500235791 23.48076907705957 50-51 23.474774400332503 26.33699275043761 26.993409852051382 23.194822997178505 52-53 23.767954056797564 26.108715460670922 26.877852467009294 23.245478015522217 54-55 23.218462005738903 26.270911430649583 27.083909488374324 23.42671707523719 56-57 23.28686126719473 26.426812990064825 26.99712655162216 23.289199191118286 58-59 23.308342192133384 25.934170456634508 26.930925338299588 23.826562012932516 60-61 22.922045223841227 26.257803070873067 27.310708091215 23.509443614070705 62-63 23.022296200973535 26.398957725539724 27.16014179408685 23.418604279399894 64-65 22.738708027271784 26.341328899936855 27.0691825658815 23.850780506909864 66-67 22.780782558183915 26.267291419427536 27.112542020244852 23.8393840021437 68-69 23.126628695982145 25.88918482923083 27.28843712526629 23.69574934952073 70-71 22.94801429053002 26.202708678485653 26.921511388003395 23.927765642980933 72-73 23.03466521728705 26.265815955431577 26.751544628370006 23.947974198911368 74-75 22.895468823684567 26.659586287376808 26.58695078770052 23.8579941012381 76-77 22.969123418404457 26.268393666424217 26.866942155366036 23.895540759805293 78-79 23.079385505670963 26.35305848406615 26.944992846352438 23.62256316391045 80-81 23.129079062346946 26.55366802634579 26.66083442721127 23.65641848409599 82-83 23.091132094398418 26.51417570836579 26.68850209195657 23.70619010527923 84-85 23.00837897841609 26.493238665997893 26.726343324294245 23.77203903129178 86-87 23.09698567462583 26.427316945281664 26.551009654225926 23.924687725866583 88-89 22.91228305755811 26.619592449741607 26.39347315322919 24.074651339471085 90-91 22.935065931615284 26.616777332025656 26.49186415777227 23.956292578586794 92-93 23.04552767190998 26.150550904432833 26.618018878160786 24.1859025454964 94-95 22.67684749479412 26.116333918746935 26.787617952781524 24.41920063367742 96-97 22.87458736641862 25.88259625452598 26.809772921645582 24.43304345740982 98-99 22.720267123061845 26.15811774955614 26.410274086884932 24.71134104049708 100-101 22.800533126583595 25.903157995699814 26.72866494552837 24.567643932188215 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 403.0 1 281.0 2 212.0 3 284.0 4 258.0 5 254.5 6 513.5 7 1096.0 8 1662.0 9 1854.5 10 1662.5 11 1470.0 12 1507.5 13 1615.5 14 1751.5 15 1934.5 16 2085.0 17 2207.0 18 2422.0 19 2691.0 20 2988.0 21 3531.0 22 4267.5 23 5116.5 24 6290.0 25 7858.5 26 9940.0 27 12687.0 28 15452.5 29 18500.0 30 22157.0 31 26996.5 32 34977.0 33 38932.5 34 38665.0 35 41454.0 36 46101.0 37 49971.0 38 53781.5 39 56970.5 40 60125.5 41 67368.0 42 72971.5 43 76746.5 44 82226.0 45 89251.0 46 93944.0 47 95935.0 48 97071.0 49 96364.0 50 96786.5 51 95827.0 52 94721.0 53 96810.0 54 99429.0 55 95756.0 56 87480.5 57 80827.0 58 75774.5 59 67226.0 60 54347.0 61 44778.0 62 38306.0 63 29673.0 64 21944.5 65 17098.0 66 12678.5 67 9732.5 68 7424.0 69 5277.0 70 4010.5 71 2953.0 72 2217.0 73 1866.0 74 1475.0 75 1147.0 76 831.0 77 501.0 78 300.5 79 182.5 80 98.0 81 53.5 82 34.0 83 18.5 84 11.0 85 8.5 86 4.5 87 3.0 88 3.0 89 2.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.394321842204123E-4 2 0.04547961410267682 3 8.79219253303067E-4 4 0.0 5 0.09275763122347355 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.9973383635331825E-4 26-27 0.009451606973007969 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011110134200829663 36-37 1.198935345413273E-4 38-39 0.00443606077802911 40-41 0.005974694471309478 42-43 4.5959188240842126E-4 44-45 1.9982255756887883E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.398757902982152E-4 68-69 0.0010590595551150579 70-71 1.398757902982152E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014587046702528154 82-83 6.79396695734188E-4 84-85 0.004995563939221971 86-87 0.002058172342959452 88-89 3.9964511513775766E-5 90-91 0.0032770899441296126 92-93 6.594144399773001E-4 94-95 1.9982255756887883E-5 96-97 0.0 98-99 9.991127878443942E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2502220.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.13224682309068 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7461478207075 36.233139369791324 2 14.00076028104805 13.757776196787544 3 4.945957951847156 7.290180806003474 4 2.311841211469292 4.54343812070809 5 1.2753277859227137 3.132985977915026 6 0.8008775441557101 2.360934790471743 7 0.5556012003834647 1.9108554718712096 8 0.37354909386432794 1.4682645024227223 9 0.27736453864530997 1.2264788675424636 >10 1.5238245390358625 13.456623596374145 >50 0.11331879119710096 3.8544726312228246 >100 0.06785388840509868 6.24957459180721 >500 0.004235681425280051 1.4487556419383893 >1k 0.003258216480983297 2.818390015806499 >5k 8.145541202458242E-5 0.24812941933743657 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6200 0.24777997138540975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4843 0.19354812926121603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3467 0.1385569614182606 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3102 0.12396991471573242 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3093 0.12361023411210845 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2841 0.11353917721063697 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2581 0.10314840421705525 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2522 0.10079049803774248 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 7.992902302755153E-5 0.0 0.0 0.0 6 3.9964511513775766E-5 7.992902302755153E-5 0.0 0.0 0.0 7 3.9964511513775766E-5 7.992902302755153E-5 0.0 0.0 0.0 8 3.9964511513775766E-5 7.992902302755153E-5 0.0 0.0 0.0 9 3.9964511513775766E-5 7.992902302755153E-5 0.0 3.9964511513775766E-5 0.0 10-11 3.9964511513775766E-5 7.992902302755153E-5 0.0 3.9964511513775766E-5 0.0 12-13 7.992902302755153E-5 7.992902302755153E-5 0.0 3.9964511513775766E-5 3.9964511513775766E-5 14-15 7.992902302755153E-5 7.992902302755153E-5 0.0 3.9964511513775766E-5 3.9964511513775766E-5 16-17 7.992902302755153E-5 7.992902302755153E-5 0.0 3.9964511513775766E-5 3.9964511513775766E-5 18-19 9.991127878443941E-5 7.992902302755153E-5 0.0 3.9964511513775766E-5 3.9964511513775766E-5 20-21 1.198935345413273E-4 7.992902302755153E-5 0.0 5.994676727066365E-5 3.9964511513775766E-5 22-23 1.5985804605510306E-4 7.992902302755153E-5 0.0 1.398757902982152E-4 3.9964511513775766E-5 24-25 2.397870690826546E-4 7.992902302755153E-5 0.0 2.9973383635331825E-4 3.9964511513775766E-5 26-27 2.797515805964304E-4 7.992902302755153E-5 0.0 9.391660205737305E-4 3.9964511513775766E-5 28-29 4.595918824084213E-4 7.992902302755153E-5 0.0 0.0031172318980745095 3.9964511513775766E-5 30-31 5.19538649679085E-4 7.992902302755153E-5 0.0 0.010730471341448793 3.9964511513775766E-5 32-33 5.395209054359729E-4 7.992902302755153E-5 0.0 0.0280351048269137 3.9964511513775766E-5 34-35 6.394321842204123E-4 7.992902302755153E-5 0.0 0.05451159370479015 3.9964511513775766E-5 36-37 7.393434630048516E-4 1.5985804605510306E-4 0.0 0.09793303546450752 5.994676727066365E-5 38-39 9.591482763306184E-4 1.9982255756887884E-4 0.0 0.1644140003676735 7.992902302755153E-5 40-41 9.591482763306184E-4 1.9982255756887884E-4 0.0 0.23656992590579567 7.992902302755153E-5 42-43 0.0010190950436012821 1.9982255756887884E-4 0.0 0.31238260424742825 7.992902302755153E-5 44-45 0.0011190063223857215 2.397870690826546E-4 0.0 0.40939645594711893 7.992902302755153E-5 46-47 0.0011190063223857215 2.597693248395425E-4 0.0 0.522036431648696 9.991127878443941E-5 48-49 0.0011589708338994972 2.9973383635331825E-4 0.0 0.642149771003349 1.198935345413273E-4 50-51 0.0011589708338994972 3.197160921102061E-4 0.0 0.7735331026048868 1.198935345413273E-4 52-53 0.0011589708338994972 3.197160921102061E-4 0.0 0.8972632302515366 1.7984030181199094E-4 54-55 0.0011589708338994972 3.197160921102061E-4 0.0 1.0383579381509218 1.9982255756887884E-4 56-57 0.001198935345413273 3.197160921102061E-4 0.0 1.2047301995827704 1.9982255756887884E-4 58-59 0.001198935345413273 3.197160921102061E-4 0.0 1.385729472228661 1.9982255756887884E-4 60-61 0.001198935345413273 3.197160921102061E-4 0.0 1.5807762706716435 1.9982255756887884E-4 62-63 0.001198935345413273 3.197160921102061E-4 0.0 1.7793998928951091 1.9982255756887884E-4 64-65 0.001198935345413273 3.596806036239819E-4 0.0 1.9848974110989444 1.9982255756887884E-4 66-67 0.0012788643684408245 3.596806036239819E-4 0.0 2.220528170984166 1.9982255756887884E-4 68-69 0.001358793391468376 3.596806036239819E-4 0.0 2.4807570877061167 1.9982255756887884E-4 70-71 0.0013987579029821518 4.196273708946455E-4 0.0 2.763625900200622 1.9982255756887884E-4 72-73 0.0014387224144959275 4.795741381653092E-4 0.0 3.0547473843227215 1.9982255756887884E-4 74-75 0.0014587046702528154 4.795741381653092E-4 0.0 3.349285834179249 1.9982255756887884E-4 76-77 0.0014786869260097035 4.795741381653092E-4 0.0 3.6798722734212017 2.397870690826546E-4 78-79 0.0014986691817665914 4.795741381653092E-4 0.0 4.041431209086332 2.397870690826546E-4 80-81 0.001718473995092358 4.795741381653092E-4 0.0 4.424550998713142 2.397870690826546E-4 82-83 0.0017984030181199095 4.795741381653092E-4 0.0 4.827693008608356 2.397870690826546E-4 84-85 0.001878332041147461 4.795741381653092E-4 0.0 5.231894078058684 2.397870690826546E-4 86-87 0.0019182965526612368 4.795741381653092E-4 0.0 5.652540543996931 2.397870690826546E-4 88-89 0.0019182965526612368 4.795741381653092E-4 0.0 6.11073366850237 2.397870690826546E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3800 0.0 49.812786 1 GTATCAA 6330 0.0 42.826492 1 ATCAACG 8180 0.0 33.009983 3 TATCAAC 8380 0.0 32.17894 2 TCAACGC 8370 0.0 32.145767 4 CAACGCA 8730 0.0 30.820173 5 AACGCAG 9065 0.0 29.655106 6 GTGGTAT 1750 0.0 28.535969 1 TAAGGTG 1445 0.0 28.291988 5 ACGCAGA 10530 0.0 25.303778 7 CGCAGAG 10635 0.0 25.053951 8 TGGTATC 2010 0.0 24.60764 2 GTACATG 8570 0.0 24.196194 1 TACATGG 8625 0.0 23.159163 2 AGGTAAG 1530 0.0 22.69155 2 ACATGGG 8640 0.0 22.39396 3 GGTAAGG 1610 0.0 22.145506 3 TAAGGTA 840 0.0 22.070807 4 GCAGAGT 12220 0.0 21.376783 9 GAGTACT 6905 0.0 20.944715 12-13 >>END_MODULE