FastQCFastQC Report
Thu 26 May 2016
SRR938601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508848
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63340.25246647066701533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.19319623986785966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34360.13695528784525807No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31560.12579478708953273No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30030.11969637060515424No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28640.114155979158562No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26260.10466955351619547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.051.7125631
GTATCAA65350.047.1061061
ATCAACG85850.035.595773
TATCAAC87050.035.2142682
TCAACGC86600.035.177744
CAACGCA88350.034.480955
AACGCAG92150.033.0015566
ACGCAGA105600.028.7475037
GTGGTAT14650.028.6243271
CGCAGAG106100.028.5224748
GTACATG83850.027.733651
TACATGG86050.026.9523532
GCAGAGT114300.025.6424089
ACATGGG89600.024.7706053
AGGTAAG12500.023.952912
TAAGGTG14300.023.9289825
TGGTATC18100.022.8438032
CATGGGG63400.022.5634234
GAGTACT68500.022.4327212-13
CAGAGTA110400.021.3836210-11
Produced by FastQC (version 0.11.5)