FastQCFastQC Report
Thu 26 May 2016
SRR938601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508848
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59900.23875499830998131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50270.20037084749654024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.14357187043615235No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32570.12982053914784794No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31270.12463887808268975No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29230.11650765610351843No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26400.10522757855398175No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26280.10474927137873638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38400.050.7862321
GTATCAA62650.043.8833851
TATCAAC82550.033.074112
ATCAACG82600.033.038923
TCAACGC83900.032.4703254
CAACGCA86950.031.3313455
AACGCAG90500.030.0705286
ATACGCG856.3748483E-427.966954
ACGCGTA856.4143655E-427.9374076
GTGGTAT16950.026.6592061
ACGCAGA104150.025.9926537
CGCAGAG103950.025.9055968
TAAGGTG12750.025.35675
TACGCGT950.001220382925.0230585
GTACATG81600.025.0069351
TACATGG81750.023.9719182
CGCGTAT1000.00165548923.7467947
ACATGGG83850.023.0206183
GCAGAGT117200.022.531099
TGGTATC19350.022.369372
Produced by FastQC (version 0.11.5)