##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938601_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2508848 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.792549807720516 31.0 31.0 34.0 30.0 34.0 2 32.030502844333334 31.0 31.0 34.0 30.0 34.0 3 32.098637302857725 33.0 31.0 34.0 30.0 34.0 4 35.77669193191457 37.0 35.0 37.0 35.0 37.0 5 35.64288470246105 37.0 35.0 37.0 33.0 37.0 6 35.67929902489111 37.0 35.0 37.0 33.0 37.0 7 35.60870487171802 37.0 35.0 37.0 33.0 37.0 8 35.589572983297515 37.0 35.0 37.0 33.0 37.0 9 37.16013126343246 39.0 37.0 39.0 33.0 39.0 10-11 37.089728433129466 39.0 37.0 39.0 33.0 39.0 12-13 36.978057658335615 39.0 37.0 39.0 33.0 39.0 14-15 38.040093700375635 40.0 37.0 41.0 33.0 41.0 16-17 37.95947821470253 40.0 37.0 41.0 33.0 41.0 18-19 37.9440412492108 40.0 37.0 41.0 33.0 41.0 20-21 37.923553559243125 40.0 37.0 41.0 32.5 41.0 22-23 37.95370245626678 40.0 37.0 41.0 33.0 41.0 24-25 37.877016861922286 40.0 37.0 41.0 32.5 41.0 26-27 37.70596464991104 40.0 37.0 41.0 32.0 41.0 28-29 37.56873931780642 39.5 37.0 41.0 32.0 41.0 30-31 37.324875600275504 39.0 36.0 41.0 31.0 41.0 32-33 37.116476765431784 39.0 36.0 41.0 31.0 41.0 34-35 36.95201801783129 39.0 36.0 40.5 30.5 41.0 36-37 36.97567289847771 39.0 36.0 41.0 31.0 41.0 38-39 36.83300801802261 39.0 35.0 41.0 30.0 41.0 40-41 36.65031161712467 39.0 35.0 40.5 30.0 41.0 42-43 36.42594728736057 39.0 35.0 40.0 30.0 41.0 44-45 36.14967168198312 38.5 35.0 40.0 30.0 41.0 46-47 35.80616083557075 38.0 35.0 40.0 28.5 41.0 48-49 35.42128140086606 38.0 34.0 40.0 27.5 41.0 50-51 35.042456537821344 38.0 34.0 40.0 26.5 41.0 52-53 34.50951950855532 38.0 33.0 40.0 25.5 41.0 54-55 34.162605905180385 37.0 33.0 40.0 24.0 41.0 56-57 33.78148158039068 37.0 33.0 40.0 23.0 41.0 58-59 33.30775698647347 36.0 32.0 39.0 22.5 41.0 60-61 32.886650566315694 36.0 31.0 39.0 22.0 40.0 62-63 32.38621749902744 35.0 31.0 39.0 20.5 40.0 64-65 31.873150944178363 35.0 30.5 38.0 18.5 40.0 66-67 31.367266570154907 35.0 30.0 38.0 17.0 40.0 68-69 30.79205117249032 34.0 29.0 37.0 13.0 39.5 70-71 30.2120814015038 34.0 29.0 36.5 8.0 39.0 72-73 29.620717556424303 34.0 28.0 36.0 7.0 39.0 74-75 28.998992366217486 33.5 27.5 35.5 4.5 38.0 76-77 27.75235685860602 31.5 26.0 34.5 2.0 36.0 78-79 28.38171842216029 33.0 27.0 35.0 2.0 37.0 80-81 28.088488023188333 33.0 26.0 35.0 2.0 36.5 82-83 27.606256536864727 33.0 26.0 35.0 2.0 36.0 84-85 27.02497301550353 32.0 25.0 35.0 2.0 35.5 86-87 26.382178992111122 32.0 24.5 34.5 2.0 35.0 88-89 25.62827919427562 31.5 22.0 34.0 2.0 35.0 90-91 25.09054016026479 31.0 20.0 34.0 2.0 35.0 92-93 24.305831002914488 31.0 16.5 34.0 2.0 35.0 94-95 23.511383511476183 31.0 4.5 34.0 2.0 35.0 96-97 22.525394722996374 30.0 2.0 34.0 2.0 35.0 98-99 21.340885338609596 29.5 2.0 34.0 2.0 35.0 100-101 18.216591439577048 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2597408112164281 1101 2 0.33822506389397944 1101 3 0.23230067018507583 1101 4 0.20916720359092977 1101 5 0.17125884772671895 1101 6 0.1543990004220248 1101 7 0.2638318647356357 1101 8 0.1413974220085663 1101 9 0.22616859788011112 1101 10-11 0.34949004597761046 1101 12-13 0.29943417127150695 1101 14-15 0.4783240378816416 1101 16-17 0.46478776537598065 1101 18-19 0.6032722684978609 1101 20-21 0.5019909773561082 1101 22-23 0.5042921790636612 1101 24-25 0.4494608196718204 1101 26-27 0.44463671157120643 1101 28-29 0.43963270563726553 1101 30-31 0.562272418666474 1101 32-33 0.6537603431280061 1101 34-35 0.3656464880119472 1101 36-37 0.49796051529451546 1101 38-39 0.7117194613292952 1101 40-41 0.8280351500831458 1101 42-43 0.6704027724124444 1101 44-45 0.8619499498493539 1101 46-47 0.9157623418640313 1101 48-49 1.0783485573096883 1101 50-51 1.209939956309391 1101 52-53 1.3576016444935846 1101 54-55 1.354402649746902 1101 56-57 1.459245582848176 1101 58-59 1.513906594938092 1101 60-61 1.449377968403489 1101 62-63 1.5651031766201058 1101 64-65 1.6208253140066518 1101 66-67 1.62281791231133 1101 68-69 1.8316243700073152 1101 70-71 1.8046238384812483 1101 72-73 1.9739147051254378 1101 74-75 1.8847094576873573 1101 76-77 1.8232453909511186 1101 78-79 1.9500829629461691 1101 80-81 2.2231220794665525 1101 82-83 2.3942072989052647 1101 84-85 2.66170690165632 1101 86-87 2.5578488453932913 1101 88-89 2.844356444054668 1101 90-91 3.1990908381866134 1101 92-93 3.2570040888129626 1101 94-95 3.4458726270132836 1101 96-97 3.7418782863145346 1101 98-99 4.229740204897023 1101 100-101 4.898001293367233 1102 1 0.40722185380907305 1102 2 0.3988073495892337 1102 3 0.39818568789989683 1102 4 0.3186504045612466 1102 5 0.19094072918071703 1102 6 0.29904634767223115 1102 7 0.3709685902543214 1102 8 0.3434586775201609 1102 9 0.5385882158501545 1102 10-11 0.45309346185888444 1102 12-13 0.4268458735273768 1102 14-15 0.7041944647445106 1102 16-17 0.5881729846241299 1102 18-19 0.6429545425596928 1102 20-21 0.6586919484008007 1102 22-23 0.6473456833058364 1102 24-25 0.6658383430018162 1102 26-27 0.5999888172445083 1102 28-29 0.606869565463235 1102 30-31 0.5500789685559155 1102 32-33 0.42040373758962346 1102 34-35 0.6779621614478231 1102 36-37 0.8016772725532988 1102 38-39 0.8986843823016812 1102 40-41 0.8777614267947556 1102 42-43 0.7506723298742202 1102 44-45 0.6729853838330726 1102 46-47 0.8800306841723682 1102 48-49 0.8887011111029963 1102 50-51 0.9820008562212621 1102 52-53 1.0195600206611317 1102 54-55 0.9785590859490085 1102 56-57 1.0697463461003451 1102 58-59 1.1588142057858448 1102 60-61 1.121605774917974 1102 62-63 1.3639385633943348 1102 64-65 1.2540127763649735 1102 66-67 1.227886366866894 1102 68-69 1.3822674021112071 1102 70-71 1.6769138186304104 1102 72-73 1.7054770606630498 1102 74-75 1.7820383245165452 1102 76-77 1.728684586375369 1102 78-79 1.9452246823078347 1102 80-81 2.2929032955307136 1102 82-83 2.195542181645049 1102 84-85 2.432460449648527 1102 86-87 2.5975521175382603 1102 88-89 2.7087011839865696 1102 90-91 2.828841936080643 1102 92-93 2.9901945600920072 1102 94-95 3.3062004577761392 1102 96-97 3.7846841258090507 1102 98-99 4.037519568302724 1102 100-101 4.459683444175951 1103 1 0.29404446701519404 1103 2 0.2829753752801807 1103 3 0.26227508644848996 1103 4 0.15132486751368646 1103 5 0.08445783195855938 1103 6 0.1878383877517038 1103 7 0.15209373429111395 1103 8 0.14541867113553764 1103 9 0.2411129242703396 1103 10-11 0.3188103316789892 1103 12-13 0.3091050700949225 1103 14-15 0.368241632283862 1103 16-17 0.4815193048257598 1103 18-19 0.4587057563107564 1103 20-21 0.3785739295973798 1103 22-23 0.3059916859315308 1103 24-25 0.42372924768562115 1103 26-27 0.42083063244073315 1103 28-29 0.4523048525325777 1103 30-31 0.39502533486181335 1103 32-33 0.4135506474635946 1103 34-35 0.42979172933901566 1103 36-37 0.5387277051868793 1103 38-39 0.6419210955422017 1103 40-41 0.5772280572693589 1103 42-43 0.5724217837916683 1103 44-45 0.5465754319333129 1103 46-47 0.638697724212804 1103 48-49 0.7378258328296994 1103 50-51 0.8573802559224646 1103 52-53 1.1422353238607243 1103 54-55 0.8618491470746505 1103 56-57 0.9063160886442461 1103 58-59 1.0894009669266893 1103 60-61 1.1874168055201508 1103 62-63 1.105495328961318 1103 64-65 1.1979882042897287 1103 66-67 1.314317187507239 1103 68-69 1.4785249756586047 1103 70-71 1.4901636572186234 1103 72-73 1.492002744894421 1103 74-75 1.5469935524624816 1103 76-77 1.4448711980834155 1103 78-79 1.7114659725526273 1103 80-81 2.0757987372367737 1103 82-83 2.264613542992244 1103 84-85 2.4477978494865376 1103 86-87 2.4341662151362478 1103 88-89 2.4996439444139895 1103 90-91 2.70502620821577 1103 92-93 2.7516223117999132 1103 94-95 2.57746089722227 1103 96-97 3.070278666264141 1103 98-99 3.5540314399954234 1103 100-101 4.046455908058601 1104 1 0.35592458762610946 1104 2 0.28314066634106183 1104 3 0.29753242794952683 1104 4 0.20016064220104823 1104 5 0.24625988666292642 1104 6 0.25782080320912115 1104 7 0.2732093185389033 1104 8 0.21492098430052664 1104 9 0.2789973321371022 1104 10-11 0.33976029125091856 1104 12-13 0.3606737580842392 1104 14-15 0.5848719450266842 1104 16-17 0.6443226594154225 1104 18-19 0.5822655252550817 1104 20-21 0.5394201773689886 1104 22-23 0.5549024330046706 1104 24-25 0.5595460311882974 1104 26-27 0.5614713369870543 1104 28-29 0.5709756455111332 1104 30-31 0.5455342190288732 1104 32-33 0.5677725147733881 1104 34-35 0.5344312826103561 1104 36-37 0.6796171045403909 1104 38-39 0.5637551044964226 1104 40-41 0.7047208436035177 1104 42-43 0.7953029798559754 1104 44-45 0.9592773372388237 1104 46-47 0.92439816707153 1104 48-49 1.1296106851322776 1104 50-51 1.1187777569642492 1104 52-53 1.3577891217033908 1104 54-55 1.3636119989853839 1104 56-57 1.588348404411441 1104 58-59 1.6555007105284147 1104 60-61 1.4787065125273458 1104 62-63 1.5844067675273337 1104 64-65 1.5803431257476674 1104 66-67 1.6539936537602635 1104 68-69 1.833036542090145 1104 70-71 1.8728653866180522 1104 72-73 1.983490103616056 1104 74-75 2.0348167571437656 1104 76-77 2.0548752803176917 1104 78-79 2.361931335128425 1104 80-81 2.7460414027113202 1104 82-83 2.822349862199914 1104 84-85 2.8719917295319313 1104 86-87 3.1235539831124832 1104 88-89 3.0792582794253036 1104 90-91 3.2075900393774717 1104 92-93 3.469189779364953 1104 94-95 3.577134561109421 1104 96-97 3.9777608618389486 1104 98-99 4.408610335996713 1104 100-101 4.504813933955614 1105 1 0.2436355191116384 1105 2 0.24993360693324718 1105 3 0.20858161373754314 1105 4 0.19091441575072565 1105 5 0.11990397791440444 1105 6 0.1606451945429228 1105 7 0.1771132215529434 1105 8 0.21656440230179186 1105 9 0.395679713901977 1105 10-11 0.29913014445747876 1105 12-13 0.3778423794088397 1105 14-15 0.4421071920461017 1105 16-17 0.554197954036475 1105 18-19 0.4544959984800556 1105 20-21 0.4099202612289403 1105 22-23 0.2860339437817032 1105 24-25 0.36315891349929785 1105 26-27 0.4450417203781569 1105 28-29 0.48873614622912953 1105 30-31 0.5781988556722908 1105 32-33 0.5173058622908115 1105 34-35 0.48308661939251607 1105 36-37 0.5306747178091129 1105 38-39 0.5950105679871172 1105 40-41 0.48709774875050726 1105 42-43 0.6374118768931396 1105 44-45 0.68366390020757 1105 46-47 0.7957464810123724 1105 48-49 0.7345646747620904 1105 50-51 0.8285752070290542 1105 52-53 0.9169046095993991 1105 54-55 0.9226631843739597 1105 56-57 0.8819206131640414 1105 58-59 1.1086130704185848 1105 60-61 1.2384415269316946 1105 62-63 1.2767293444636536 1105 64-65 1.4065687107967761 1105 66-67 1.4720759337078952 1105 68-69 1.491544593534524 1105 70-71 1.4162778762392705 1105 72-73 1.5906914214322398 1105 74-75 1.742610161780778 1105 76-77 1.658608114955939 1105 78-79 1.8834532658393606 1105 80-81 2.096811877133444 1105 82-83 2.2495369030814913 1105 84-85 2.352761723907687 1105 86-87 2.4088076105739837 1105 88-89 2.598559847517514 1105 90-91 2.7658414438094283 1105 92-93 3.065366328566448 1105 94-95 3.14221447697685 1105 96-97 3.7011828871960653 1105 98-99 3.9242817756676267 1105 100-101 4.531274267605621 1106 1 0.12443254798554904 1106 2 0.23508006612340182 1106 3 0.15841117281213712 1106 4 0.09267214096813348 1106 5 0.0834818014588663 1106 6 0.13250773966073126 1106 7 0.1482545384094749 1106 8 0.11045817218519716 1106 9 0.10549280652338666 1106 10-11 0.23817568423086044 1106 12-13 0.08494467172659625 1106 14-15 0.05743893465716354 1106 16-17 0.058810486392488315 1106 18-19 0.2439083914861726 1106 20-21 0.4540190706948195 1106 22-23 0.31716982455932907 1106 24-25 0.305440278495432 1106 26-27 0.1728470848715773 1106 28-29 0.3203539006240348 1106 30-31 0.43577731984417056 1106 32-33 0.3525215486472604 1106 34-35 0.3650324341341715 1106 36-37 0.47952654391070126 1106 38-39 0.39145538432318006 1106 40-41 0.38616676426497776 1106 42-43 0.6072764913629101 1106 44-45 0.1580082795378175 1106 46-47 0.07700393472435252 1106 48-49 0.34626176725095803 1106 50-51 0.3063777355669757 1106 52-53 0.37012171751587175 1106 54-55 0.6947384112280091 1106 56-57 0.4926346739209535 1106 58-59 0.9311325571643749 1106 60-61 0.7953886327794066 1106 62-63 0.9367539545869406 1106 64-65 0.6569949996863045 1106 66-67 0.5138517970996226 1106 68-69 0.5034488898876894 1106 70-71 0.6169503857703162 1106 72-73 1.041250044960936 1106 74-75 1.4611083681135533 1106 76-77 1.494064036595205 1106 78-79 1.6987996509364471 1106 80-81 1.641898718568605 1106 82-83 2.0886433711198293 1106 84-85 2.0765868994906462 1106 86-87 2.236587944028617 1106 88-89 2.497394598181799 1106 90-91 2.472933213239674 1106 92-93 1.9325115154574064 1106 94-95 2.6193121959432126 1106 96-97 3.1681142281164263 1106 98-99 3.5394587348128503 1106 100-101 3.9713182876053743 1107 1 0.10374138571651059 1107 2 0.08311621923569135 1107 3 0.14522464150296344 1107 4 0.18155691701898746 1107 5 0.016123739776134016 1107 6 0.05134948331772904 1107 7 0.08847779734605155 1107 8 0.12376054139902237 1107 9 0.1539819983210151 1107 10-11 0.1783513955785736 1107 12-13 0.13621090564934235 1107 14-15 0.30117495979266806 1107 16-17 0.2786982185740854 1107 18-19 0.30264682759849393 1107 20-21 0.31652101167357927 1107 22-23 0.27639393263302736 1107 24-25 0.29521177690766365 1107 26-27 0.29819348475950846 1107 28-29 0.34748502226654665 1107 30-31 0.27777164247216746 1107 32-33 0.4384744424986948 1107 34-35 0.4774461820045701 1107 36-37 0.3977604672382782 1107 38-39 0.38760921530764847 1107 40-41 0.3771265141719624 1107 42-43 0.5158227577962222 1107 44-45 0.4884814781086675 1107 46-47 0.564539966082684 1107 48-49 0.5889965175852154 1107 50-51 0.5260987225176095 1107 52-53 0.626865331421854 1107 54-55 0.6555733673388815 1107 56-57 0.5764954008249887 1107 58-59 0.6785641474318282 1107 60-61 0.8165868962917955 1107 62-63 0.9917574582088164 1107 64-65 0.96872853218877 1107 66-67 1.076314063961231 1107 68-69 1.236645491644719 1107 70-71 1.2508926009153427 1107 72-73 1.3573605849978314 1107 74-75 1.442310490941761 1107 76-77 1.4754051503014303 1107 78-79 1.793446814960145 1107 80-81 1.8999789236820348 1107 82-83 1.8963431736110863 1107 84-85 2.1316699412450255 1107 86-87 2.3098268609485864 1107 88-89 2.702210036847383 1107 90-91 2.67559659398572 1107 92-93 2.9365895063577767 1107 94-95 2.9999406509036675 1107 96-97 3.152563610238797 1107 98-99 3.37262074588158 1107 100-101 3.81245726489837 1108 1 0.12433292446604227 1108 2 0.17222142327912593 1108 3 0.08045489234534386 1108 4 0.09394927943007048 1108 5 0.02922744625310969 1108 6 0.06894588915824329 1108 7 0.16447929491250335 1108 8 0.10915926846753621 1108 9 0.1625930054344522 1108 10-11 0.13904027614765369 1108 12-13 0.2784797188279313 1108 14-15 0.35227941505287674 1108 16-17 0.2891811837556659 1108 18-19 0.31612492568910255 1108 20-21 0.3444131868888505 1108 22-23 0.2632902261560588 1108 24-25 0.29446299368040485 1108 26-27 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0.544061251363317 2316 72-73 0.7696747329232956 2316 74-75 0.8216508944691974 2316 76-77 0.9263296751762304 2316 78-79 0.945567168889955 2316 80-81 0.9396045320684081 2316 82-83 1.0995897790653437 2316 84-85 1.442737179754726 2316 86-87 1.4784522506309479 2316 88-89 1.656307845729728 2316 90-91 1.8207479260414274 2316 92-93 1.7996772363213367 2316 94-95 2.232898455856759 2316 96-97 2.384543845327073 2316 98-99 2.785677004708514 2316 100-101 3.093309765723145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 17.0 4 51.0 5 76.0 6 198.0 7 463.0 8 1108.0 9 1915.0 10 3066.0 11 4769.0 12 6833.0 13 9494.0 14 12719.0 15 16432.0 16 20385.0 17 25395.0 18 25120.0 19 23174.0 20 23932.0 21 25598.0 22 28098.0 23 31761.0 24 36761.0 25 42580.0 26 50147.0 27 60081.0 28 71481.0 29 84751.0 30 99635.0 31 117534.0 32 141012.0 33 170571.0 34 211955.0 35 267086.0 36 336152.0 37 362044.0 38 186073.0 39 10373.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.56328003928496 18.643576653507903 14.884042397148015 23.90910091005912 2 16.321938268301565 19.93800750720873 38.53112963433362 25.20892459015609 3 19.541301956282584 23.17931793179318 29.43679150523748 27.842588606686757 4 13.9825529486043 16.379031332308696 35.043494065802314 34.59492165328469 5 14.50338033024454 36.710338575664814 33.58764280219482 15.198638291895824 6 34.01943840360197 35.01511450673775 16.369305753078702 14.596141336581569 7 30.18696230301716 31.78705923993801 19.5237814327532 18.50219702429163 8 27.338124908324456 34.629359769902365 18.884922482350465 19.147592839422714 9 26.63959713780986 16.645408569989094 19.1952242622909 37.51977002991014 10-11 25.797676862049833 25.943919280881104 26.62118231156292 21.637221545506144 12-13 26.447516948017576 22.971280045662393 27.108059157031434 23.473143849288597 14-15 22.934689546756122 24.72264162675459 25.02790125188931 27.314767574599973 16-17 23.064888745751038 28.02856131579115 25.859039686740687 23.047510251717124 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25.771551913134637 27.45903272996879 23.561730929441822 44-45 23.125638259962837 25.935277549910836 27.64659152239301 23.29249266773332 46-47 23.441784214023414 26.299969667341212 27.009080266752015 23.249165851883355 48-49 23.371463715617686 26.120853076790624 27.001615881073704 23.506067326517986 50-51 23.455506272201422 26.37427616180813 26.96165730247508 23.208560263515366 52-53 23.76150328756465 26.053092893630865 26.931583738831527 23.253820079972957 54-55 23.23215675082747 26.29146923209377 27.013214830073405 23.46315918700535 56-57 23.300156087574855 26.422844269561168 26.975289056969576 23.3017105858944 58-59 23.277117625300537 25.943221749583874 26.931583738831527 23.848076886284066 60-61 22.9116510844818 26.306276825060742 27.288022231717505 23.49404985873995 62-63 23.003785004113443 26.41361692697206 27.141441011970436 23.441157056944064 64-65 22.712256780801386 26.3339189938968 27.058275351874645 23.895548873427167 66-67 22.79754229535902 26.255806954993233 27.091410589334934 23.855240160312814 68-69 23.159852864359905 25.88110887036905 27.241218705924624 23.717819559346413 70-71 22.996989056331827 26.167049578133074 26.889572425272473 23.946388940262622 72-73 23.036852578723895 26.27604906236828 26.704851530433793 23.98224682847403 74-75 22.924525519282156 26.65133559306901 26.57018280900238 23.853956078646455 76-77 23.005140207776638 26.290691982934 26.858203446362634 23.84596436292673 78-79 23.078460711848624 26.37501355203663 26.912471381287347 23.634054354827395 80-81 23.144560657656815 26.558280867322893 26.64192488460334 23.65523359041695 82-83 23.124032166452423 26.558847269139935 26.66610863651311 23.651011927894533 84-85 23.013546067331532 26.49645608315424 26.689362031190612 23.800635818323617 86-87 23.14668940250465 26.4470928400645 26.545725082504106 23.860492674926743 88-89 22.987810735369674 26.64704144971774 26.314538112527387 24.050609702385202 90-91 22.966655552581614 26.660610719305843 26.49172711486656 23.881006613245983 92-93 23.043026034127703 26.210563720346624 26.64307410412336 24.103336141402316 94-95 22.70873379111325 26.136562704588464 26.779009086841665 24.37569441745662 96-97 22.91087383532203 25.9168152076172 26.78386653954325 24.388444417517523 98-99 22.715764366753188 26.18554412224256 26.428663673526653 24.670027837477598 100-101 22.86924868290068 25.856839499726465 26.7361745676018 24.53773724977106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 416.0 1 280.5 2 199.0 3 307.0 4 302.5 5 278.5 6 473.5 7 1066.0 8 1688.5 9 1939.0 10 1762.5 11 1520.5 12 1558.0 13 1687.5 14 1814.0 15 1943.0 16 2116.5 17 2295.0 18 2509.0 19 2750.0 20 3040.5 21 3563.5 22 4294.5 23 5165.0 24 6286.0 25 7837.5 26 10054.5 27 12811.0 28 15590.0 29 18694.0 30 22125.5 31 27035.0 32 34983.0 33 38703.5 34 38480.0 35 41388.5 36 45795.5 37 49883.0 38 53874.0 39 56997.5 40 60757.5 41 68332.5 42 73510.5 43 76885.5 44 82359.5 45 89074.5 46 93758.5 47 96254.5 48 97040.0 49 96088.0 50 96996.0 51 96005.0 52 95089.5 53 97100.0 54 99483.5 55 95414.5 56 87010.0 57 80825.5 58 75769.5 59 67449.5 60 54890.0 61 45300.0 62 38492.0 63 29834.0 64 22399.0 65 17444.5 66 12891.5 67 9894.0 68 7607.5 69 5456.0 70 4036.0 71 2994.0 72 2337.0 73 1953.5 74 1531.5 75 1151.0 76 813.0 77 537.0 78 336.0 79 185.5 80 103.5 81 65.5 82 38.5 83 22.5 84 14.0 85 9.5 86 4.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.044841297679253586 3 3.1887145016358103E-4 4 0.0 5 0.10299547840283668 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010562616786668623 26-27 0.011897890984228618 28-29 0.0 30-31 0.0 32-33 4.3844824397492395E-4 34-35 0.015764207317462038 36-37 0.0032086439672710347 38-39 0.01259542228146145 40-41 0.007134748697410125 42-43 0.005500532515321773 44-45 0.0029495609140131247 46-47 3.985893127044763E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1957679381134289E-4 68-69 2.590830532579096E-4 70-71 0.0 72-73 1.9929465635223814E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.9788396905671447E-5 82-83 9.167554192202956E-4 84-85 0.004484129767925359 86-87 0.0013352741975599957 88-89 3.985893127044763E-5 90-91 7.772491597737288E-4 92-93 2.590830532579096E-4 94-95 1.9929465635223814E-5 96-97 0.0 98-99 0.0 100-101 8.171080910441765E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2508848.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.004472371751135 #Duplication Level Percentage of deduplicated Percentage of total 1 73.56259888417968 36.048963446139936 2 14.158610291610483 13.876704537152342 3 4.9143823447469135 7.22480141512115 4 2.354974468685758 4.616171251475622 5 1.2815951618551025 3.140194735044915 6 0.8046758325318343 2.3659628762113267 7 0.5286301834561375 1.8133670254035017 8 0.3893007545930292 1.5261982458204773 9 0.28782291909904656 1.2694149258251388 >10 1.5280195797795968 13.481141789203294 >50 0.11497272780892798 3.9159518295912696 >100 0.06691740450143445 6.208747902878923 >500 0.004157302225617882 1.409387238696045 >1k 0.0031791134666473275 2.6629038066413315 >5k 1.6303145982806804E-4 0.44008897479474884 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5990 0.23875499830998131 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5027 0.20037084749654024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3602 0.14357187043615235 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3257 0.12982053914784794 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3127 0.12463887808268975 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2923 0.11650765610351843 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2640 0.10522757855398175 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2628 0.10474927137873638 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.985893127044763E-5 7 3.985893127044763E-5 0.0 0.0 0.0 3.985893127044763E-5 8 3.985893127044763E-5 0.0 0.0 0.0 3.985893127044763E-5 9 3.985893127044763E-5 0.0 0.0 0.0 3.985893127044763E-5 10-11 3.985893127044763E-5 0.0 0.0 0.0 3.985893127044763E-5 12-13 3.985893127044763E-5 0.0 0.0 5.9788396905671447E-5 3.985893127044763E-5 14-15 7.971786254089526E-5 0.0 0.0 7.971786254089526E-5 3.985893127044763E-5 16-17 9.964732817611908E-5 0.0 0.0 7.971786254089526E-5 7.971786254089526E-5 18-19 1.395062594465667E-4 0.0 0.0 7.971786254089526E-5 1.1957679381134289E-4 20-21 1.5943572508179052E-4 3.985893127044763E-5 0.0 7.971786254089526E-5 1.1957679381134289E-4 22-23 1.9929465635223816E-4 7.971786254089526E-5 0.0 2.3915358762268579E-4 1.395062594465667E-4 24-25 2.3915358762268579E-4 7.971786254089526E-5 0.0 5.181661065158192E-4 1.5943572508179052E-4 26-27 2.590830532579096E-4 7.971786254089526E-5 0.0 0.0010761911443020861 1.5943572508179052E-4 28-29 3.188714501635811E-4 7.971786254089526E-5 0.0 0.0030890671734596915 1.5943572508179052E-4 30-31 3.587303814340287E-4 7.971786254089526E-5 0.0 0.01128007754953668 1.5943572508179052E-4 32-33 3.587303814340287E-4 7.971786254089526E-5 0.0 0.029296314483779007 1.5943572508179052E-4 34-35 4.3844824397492395E-4 7.971786254089526E-5 0.0 0.05368998042129296 1.5943572508179052E-4 36-37 5.181661065158192E-4 1.1957679381134289E-4 0.0 0.09655826100265939 1.5943572508179052E-4 38-39 5.580250377862668E-4 1.1957679381134289E-4 0.0 0.16429851469678514 1.7936519071701435E-4 40-41 5.580250377862668E-4 1.1957679381134289E-4 0.0 0.23608444991486133 1.9929465635223816E-4 42-43 5.779545034214906E-4 1.1957679381134289E-4 0.0 0.31295239886991955 1.9929465635223816E-4 44-45 6.776018315976098E-4 1.1957679381134289E-4 0.0 0.40905228216296885 1.9929465635223816E-4 46-47 8.171080910441765E-4 1.1957679381134289E-4 0.0 0.5203384182700586 1.9929465635223816E-4 48-49 9.167554192202955E-4 1.1957679381134289E-4 0.0 0.6402938719284708 1.9929465635223816E-4 50-51 9.167554192202955E-4 1.1957679381134289E-4 0.0 0.7737216443562942 2.790125188931334E-4 52-53 9.167554192202955E-4 1.1957679381134289E-4 0.0 0.9048774577017021 2.790125188931334E-4 54-55 9.167554192202955E-4 1.9929465635223816E-4 0.0 1.04759236111554 2.790125188931334E-4 56-57 0.0010363322130316384 1.9929465635223816E-4 0.0 1.2129072785597215 2.790125188931334E-4 58-59 0.0010363322130316384 1.9929465635223816E-4 0.0 1.3944846399622457 2.989419845283572E-4 60-61 0.0010562616786668623 1.9929465635223816E-4 0.0 1.5875214441050236 3.1887145016358103E-4 62-63 0.0010761911443020861 1.9929465635223816E-4 0.0 1.7830095725209338 3.3880091579880484E-4 64-65 0.0011160500755725336 1.9929465635223816E-4 0.0 1.991571430393551 3.587303814340287E-4 66-67 0.0011559090068429814 2.3915358762268579E-4 0.0 2.2346710522120112 3.587303814340287E-4 68-69 0.0011559090068429814 2.3915358762268579E-4 0.0 2.505592208057244 3.587303814340287E-4 70-71 0.0012156974037486527 2.3915358762268579E-4 0.0 2.7872952048111324 3.587303814340287E-4 72-73 0.0012356268693838766 2.3915358762268579E-4 0.0 3.082171578349904 3.9858931270447633E-4 74-75 0.0012356268693838766 2.790125188931334E-4 0.0 3.3814922227253303 4.3844824397492395E-4 76-77 0.0012356268693838766 2.790125188931334E-4 0.0 3.6998853657136666 4.7830717524537157E-4 78-79 0.0012356268693838766 2.790125188931334E-4 0.0 4.058476240888249 4.7830717524537157E-4 80-81 0.0013153447319247719 2.790125188931334E-4 0.0 4.443015280319892 4.7830717524537157E-4 82-83 0.0013751331288304434 2.790125188931334E-4 0.0 4.848181316683991 4.7830717524537157E-4 84-85 0.0014548509913713385 2.790125188931334E-4 0.0 5.257213669381326 4.7830717524537157E-4 86-87 0.00151463938827701 2.790125188931334E-4 0.0 5.674775833370536 4.7830717524537157E-4 88-89 0.00151463938827701 2.790125188931334E-4 0.0 6.121913324362417 4.7830717524537157E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3840 0.0 50.786232 1 GTATCAA 6265 0.0 43.883385 1 TATCAAC 8255 0.0 33.07411 2 ATCAACG 8260 0.0 33.03892 3 TCAACGC 8390 0.0 32.470325 4 CAACGCA 8695 0.0 31.331345 5 AACGCAG 9050 0.0 30.070528 6 ATACGCG 85 6.3748483E-4 27.96695 4 ACGCGTA 85 6.4143655E-4 27.937407 6 GTGGTAT 1695 0.0 26.659206 1 ACGCAGA 10415 0.0 25.992653 7 CGCAGAG 10395 0.0 25.905596 8 TAAGGTG 1275 0.0 25.3567 5 TACGCGT 95 0.0012203829 25.023058 5 GTACATG 8160 0.0 25.006935 1 TACATGG 8175 0.0 23.971918 2 CGCGTAT 100 0.001655489 23.746794 7 ACATGGG 8385 0.0 23.020618 3 GCAGAGT 11720 0.0 22.53109 9 TGGTATC 1935 0.0 22.36937 2 >>END_MODULE