FastQCFastQC Report
Thu 26 May 2016
SRR938600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525342
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59660.23624522935903336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45210.17902525677710188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.12726989057323723No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30370.12026093891441239No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29660.11744943853149395No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28740.11380636761278275No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25950.10275835906582158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35650.055.539861
GTATCAA63800.046.8133661
ATCAACG82750.035.4918943
TCAACGC83000.035.2704774
TATCAAC84200.034.9935722
CAACGCA85050.034.4196555
AACGCAG87450.033.5233966
ACGCAGA102000.028.454567
GTGGTAT14950.027.7647821
CGCAGAG104800.027.694328
GTACATG81450.025.9494931
TACATGG80950.025.772492
GCAGAGT116550.024.2080159
TGGTATC15900.023.6123222
ACATGGG84550.023.4947813
GTGTAAG9450.021.7096521
GAGTACT69450.021.6812812-13
CATGGGG58700.021.2924864
CAGAGTA109200.021.13990210-11
GTACTTT72450.020.68782214-15
Produced by FastQC (version 0.11.5)