FastQCFastQC Report
Thu 26 May 2016
SRR938600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525342
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62240.24646166737020175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49690.19676542820734774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34430.13633796927307273No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30820.12204287577682547No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29700.11760783291926401No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29390.1163802764140461No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26120.10343153521384431No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26090.10331273942301676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.050.4660381
GTATCAA62450.043.5967831
ATCAACG83900.032.2127463
TATCAAC84900.032.0602842
TCAACGC86000.031.4261514
CAACGCA87150.030.9569635
AACGCAG91600.029.4006126
GTGGTAT15900.026.29771
ACGCAGA107100.024.923887
TAAGGTG13150.024.9230255
CGCAGAG107400.024.7215888
CGTTACG500.001654207723.75052542-43
GTACATG85550.023.3826831
TGGTATC18250.022.1246532
TACATGG88150.022.0943872
ACATGGG86950.021.140673
GCAGAGT121450.021.040389
AGGTAAG13950.020.771892
GAGTACT69450.020.414612-13
GGTAAGG15050.020.1985823
Produced by FastQC (version 0.11.5)