##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938600_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2525342 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807613780628525 31.0 31.0 34.0 30.0 34.0 2 32.064884281020156 33.0 31.0 34.0 30.0 34.0 3 32.12439542842118 33.0 31.0 34.0 30.0 34.0 4 35.785641707143036 37.0 35.0 37.0 35.0 37.0 5 35.66883851771364 37.0 35.0 37.0 33.0 37.0 6 35.70242683961222 37.0 35.0 37.0 33.0 37.0 7 35.617916305989446 37.0 35.0 37.0 33.0 37.0 8 35.58772633568047 37.0 35.0 37.0 33.0 37.0 9 37.13054350658248 39.0 37.0 39.0 33.0 39.0 10-11 37.057225120399536 39.0 37.0 39.0 33.0 39.0 12-13 36.93315598441716 39.0 37.0 39.0 33.0 39.0 14-15 37.959417377923465 40.0 37.0 41.0 33.0 41.0 16-17 38.04872389561493 40.0 37.0 41.0 33.0 41.0 18-19 38.171065146819714 40.0 37.5 41.0 33.0 41.0 20-21 38.108158023744906 40.0 37.0 41.0 33.0 41.0 22-23 37.98551839711215 40.0 37.0 41.0 33.0 41.0 24-25 37.85866924163143 40.0 37.0 41.0 32.0 41.0 26-27 37.647027808510686 40.0 37.0 41.0 32.0 41.0 28-29 37.49902171666253 39.5 36.5 41.0 32.0 41.0 30-31 37.23961091210616 39.0 36.0 41.0 31.0 41.0 32-33 37.15234174222739 39.0 36.0 41.0 31.0 41.0 34-35 37.02464101891942 39.0 36.0 41.0 31.0 41.0 36-37 36.897985104591775 39.0 36.0 41.0 30.5 41.0 38-39 36.6753198180682 39.0 35.0 40.0 30.0 41.0 40-41 36.44771361661114 39.0 35.0 40.0 30.0 41.0 42-43 36.184322757076075 38.0 35.0 40.0 30.0 41.0 44-45 35.89456893363355 38.0 35.0 40.0 29.0 41.0 46-47 35.48813230049633 38.0 34.0 40.0 27.5 41.0 48-49 35.07084505781791 38.0 34.0 40.0 26.5 41.0 50-51 34.664629582844626 38.0 33.0 40.0 25.5 41.0 52-53 34.03942574906686 37.0 33.0 40.0 24.0 41.0 54-55 33.58210650280239 37.0 32.0 39.5 23.0 41.0 56-57 33.16808119454711 36.0 32.0 39.0 22.0 40.0 58-59 32.60799052167984 36.0 31.0 39.0 20.0 40.0 60-61 32.09567575401668 35.0 31.0 39.0 18.0 40.0 62-63 31.557710995184017 35.0 30.0 38.0 15.5 40.0 64-65 30.874604904999003 34.5 29.0 38.0 10.0 40.0 66-67 30.20309526392861 34.0 28.0 37.5 7.5 39.5 68-69 29.460724725601523 34.0 27.5 36.5 4.5 39.0 70-71 28.67264909069742 33.0 26.0 36.0 2.0 39.0 72-73 28.0798270095694 33.0 26.0 35.5 2.0 38.0 74-75 27.362025420715295 32.0 25.0 35.0 2.0 37.5 76-77 26.242640600758236 30.5 24.0 34.0 2.0 36.0 78-79 26.734437355415622 32.0 25.0 34.5 2.0 36.0 80-81 26.359290741610444 32.0 24.0 35.0 2.0 36.0 82-83 25.680834912657375 31.0 21.0 34.0 2.0 35.5 84-85 24.89953321173924 31.0 19.0 34.0 2.0 35.0 86-87 23.985620957478233 30.5 13.5 34.0 2.0 35.0 88-89 23.004022029491452 29.5 4.5 34.0 2.0 35.0 90-91 22.139793144849293 29.0 2.0 34.0 2.0 35.0 92-93 21.050879841225466 28.5 2.0 33.5 2.0 35.0 94-95 19.860018761815233 26.5 2.0 33.0 2.0 34.0 96-97 18.47039668290473 25.0 2.0 32.5 2.0 34.0 98-99 16.693554179988297 11.0 2.0 32.0 2.0 34.0 100-101 12.515997437178807 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26629196468836014 1101 2 0.2472894321469994 1101 3 0.20356281068652038 1101 4 0.16938024253428807 1101 5 0.07863658771091053 1101 6 0.23158847389679238 1101 7 0.24828561212499523 1101 8 0.2498497852450825 1101 9 0.20166287426012275 1101 10-11 0.26435844523694385 1101 12-13 0.3689482197272653 1101 14-15 0.4518384062022278 1101 16-17 0.4889805351484924 1101 18-19 0.3500316951417446 1101 20-21 0.39147312816314184 1101 22-23 0.31451916455365136 1101 24-25 0.3310553028025609 1101 26-27 0.40798695764164705 1101 28-29 0.4361083980402256 1101 30-31 0.4241304008893749 1101 32-33 0.4022467980421993 1101 34-35 0.32863380640625195 1101 36-37 0.3648717734163256 1101 38-39 0.4671210345029948 1101 40-41 0.5521383488638776 1101 42-43 0.6137861177270949 1101 44-45 0.7151017076419208 1101 46-47 0.736183134315965 1101 48-49 0.6789380427507723 1101 50-51 0.6711890008778738 1101 52-53 0.8095023016155949 1101 54-55 0.8583123741352949 1101 56-57 0.8966810813041235 1101 58-59 1.041420389361285 1101 60-61 0.9246187978758797 1101 62-63 1.0763700253293003 1101 64-65 1.0583764054542613 1101 66-67 1.0972470546739288 1101 68-69 1.1097024525813346 1101 70-71 1.461086137929744 1101 72-73 1.6043827456942914 1101 74-75 1.550909328815738 1101 76-77 1.4827018166461947 1101 78-79 1.526678314268434 1101 80-81 1.7134521804455431 1101 82-83 1.9551382722597275 1101 84-85 2.153378955762772 1101 86-87 1.9933193883540277 1101 88-89 1.8926156627894812 1101 90-91 2.0867752230962964 1101 92-93 2.531532129104729 1101 94-95 2.304715882558778 1101 96-97 2.3000628847320677 1101 98-99 2.2490428314356805 1101 100-101 2.05963436298409 1102 1 0.24609109411899865 1102 2 0.18398419060137883 1102 3 0.23545500512152984 1102 4 0.14468591285864818 1102 5 0.18606761705271424 1102 6 0.20023047706839492 1102 7 0.21931334277639536 1102 8 0.19937916990723892 1102 9 0.26330724394811256 1102 10-11 0.347886109295267 1102 12-13 0.33854189859879114 1102 14-15 0.48347980704949123 1102 16-17 0.39182631186357497 1102 18-19 0.43597398404207155 1102 20-21 0.2942161497326836 1102 22-23 0.3930496836390702 1102 24-25 0.36743893837162744 1102 26-27 0.41414814971111724 1102 28-29 0.24837757228760182 1102 30-31 0.3951501403707809 1102 32-33 0.4742241541337435 1102 34-35 0.6005256166193504 1102 36-37 0.5664080314532995 1102 38-39 0.6070520715130883 1102 40-41 0.7503274367355957 1102 42-43 0.7646799099117985 1102 44-45 0.6416533305194037 1102 46-47 0.5288460940483759 1102 48-49 0.7102007912902906 1102 50-51 0.7437595600838023 1102 52-53 0.753718123260434 1102 54-55 0.7084788245560603 1102 56-57 0.8314963004308851 1102 58-59 0.9828340547785857 1102 60-61 1.116031696463736 1102 62-63 1.186992598818609 1102 64-65 1.2222789344900598 1102 66-67 1.3270909427284536 1102 68-69 1.4916586651671189 1102 70-71 1.5686840132148632 1102 72-73 1.6593730537772977 1102 74-75 1.5699154791107581 1102 76-77 1.5946449511576084 1102 78-79 1.6198660920725807 1102 80-81 1.8571604578608678 1102 82-83 1.863928995140963 1102 84-85 2.133558755473377 1102 86-87 2.2150637816603655 1102 88-89 1.7994947202253293 1102 90-91 2.199247469924323 1102 92-93 2.458302903765521 1102 94-95 2.4113571582917537 1102 96-97 2.3491063093955944 1102 98-99 2.3441584991818836 1102 100-101 2.162014811448552 1103 1 0.14949862711994655 1103 2 0.16027265906279098 1103 3 0.15318346567428165 1103 4 0.05741829976384594 1103 5 0.08076303096114401 1103 6 0.2085881985529099 1103 7 0.1473487116576777 1103 8 0.1423416727750606 1103 9 0.2572330554719997 1103 10-11 0.304273053660431 1103 12-13 0.27926003872013894 1103 14-15 0.3563867627553847 1103 16-17 0.24081772424995762 1103 18-19 0.29682069588510984 1103 20-21 0.2298918180985865 1103 22-23 0.24641837279692425 1103 24-25 0.2936808688313519 1103 26-27 0.34301192300109307 1103 28-29 0.3977305168654155 1103 30-31 0.2942321573035187 1103 32-33 0.36554649554547325 1103 34-35 0.3791096489553354 1103 36-37 0.4571465494371125 1103 38-39 0.383114285080552 1103 40-41 0.3739021521449857 1103 42-43 0.45913577901597336 1103 44-45 0.39418587447869413 1103 46-47 0.4505883406680766 1103 48-49 0.47761477059719937 1103 50-51 0.5014847284693005 1103 52-53 0.65683048035892 1103 54-55 0.6376205201807394 1103 56-57 1.0490036355287415 1103 58-59 1.160507331893264 1103 60-61 1.0062034114310592 1103 62-63 0.9429236293135865 1103 64-65 1.1694669293643472 1103 66-67 0.959054156256741 1103 68-69 0.8982040159368161 1103 70-71 0.9687415561948995 1103 72-73 1.1023392871462612 1103 74-75 1.1811711471409225 1103 76-77 1.2250642035278752 1103 78-79 1.262489678912118 1103 80-81 1.5178221834787742 1103 82-83 1.8484250324161309 1103 84-85 1.8669918330808564 1103 86-87 1.8798043439588064 1103 88-89 1.764547851887773 1103 90-91 2.0920904568619747 1103 92-93 2.38004888088636 1103 94-95 2.0603575705121315 1103 96-97 2.362245134399654 1103 98-99 2.4439629276597827 1103 100-101 1.8952291884268835 1104 1 0.12349000885834727 1104 2 0.18794578822966912 1104 3 0.1756036830683172 1104 4 0.0856674447846899 1104 5 -0.016844821392226095 1104 6 0.08850765500204716 1104 7 0.13621604293271616 1104 8 0.06718842235930822 1104 9 0.03869536134135387 1104 10-11 0.1127078662467298 1104 12-13 0.0837586433056714 1104 14-15 0.2755054958733183 1104 16-17 0.3154735200189265 1104 18-19 0.3478168609620127 1104 20-21 0.2415594654722426 1104 22-23 0.26564344385332817 1104 24-25 0.04755256843841238 1104 26-27 0.3385044830828505 1104 28-29 0.25678034933793725 1104 30-31 0.22550358410660465 1104 32-33 0.3560519513260516 1104 34-35 0.3143648988771517 1104 36-37 0.39118062481880145 1104 38-39 0.3170596333899951 1104 40-41 0.35299634964860616 1104 42-43 0.37330321260746047 1104 44-45 0.19348718055316283 1104 46-47 0.2023841855800299 1104 48-49 0.33786716510691406 1104 50-51 0.26320395265832275 1104 52-53 0.32124032498264654 1104 54-55 0.49192745336051047 1104 56-57 0.7031870683978596 1104 58-59 0.7624338081712168 1104 60-61 0.5772653496548941 1104 62-63 0.6816034900771122 1104 64-65 0.8459648097814814 1104 66-67 0.704831136313107 1104 68-69 0.9427574145350022 1104 70-71 1.248619033601262 1104 72-73 1.4528601083328816 1104 74-75 1.2348768142518978 1104 76-77 1.2350402061064152 1104 78-79 1.5365066846103872 1104 80-81 1.6599649714254099 1104 82-83 1.6304377899787887 1104 84-85 1.826488559971306 1104 86-87 1.9529677010384 1104 88-89 1.6863399085200115 1104 90-91 1.9278040228501006 1104 92-93 2.0483479911339693 1104 94-95 1.9808779970239918 1104 96-97 2.149884100280019 1104 98-99 2.3435168554809422 1104 100-101 2.125876296567169 1105 1 0.11080872387830709 1105 2 0.16189266931846902 1105 3 0.11111118220124894 1105 4 0.08866170569051945 1105 5 0.16628870398297835 1105 6 0.08252844967256578 1105 7 0.19586129105393013 1105 8 0.20270681895320308 1105 9 0.2512504785673997 1105 10-11 0.25633576651350864 1105 12-13 0.28748582904060527 1105 14-15 0.4212483081720393 1105 16-17 0.31788874744201934 1105 18-19 0.26831840107532656 1105 20-21 0.23417742378211415 1105 22-23 0.2718631132339695 1105 24-25 0.25256232321871863 1105 26-27 0.25019432496944916 1105 28-29 0.37969679220905306 1105 30-31 0.39920127752912293 1105 32-33 0.4510296051789382 1105 34-35 0.47783373009199437 1105 36-37 0.4633111588974259 1105 38-39 0.3478982480629753 1105 40-41 0.4456361311121668 1105 42-43 0.4616720222108839 1105 44-45 0.5149202136087467 1105 46-47 0.5513556812381921 1105 48-49 0.6307384752682736 1105 50-51 0.7489990699464713 1105 52-53 0.6087756199812873 1105 54-55 0.6250992962976056 1105 56-57 0.8993049467412604 1105 58-59 1.0149374363058072 1105 60-61 0.8820797547431596 1105 62-63 0.9937172883659606 1105 64-65 1.1531076296180807 1105 66-67 1.1654313803202463 1105 68-69 1.171284934646959 1105 70-71 1.393837652619112 1105 72-73 1.6527626663989103 1105 74-75 1.5096226260343926 1105 76-77 1.5008833428022186 1105 78-79 1.747225537316659 1105 80-81 1.6346391733381402 1105 82-83 1.8208228557143329 1105 84-85 2.1258952412159147 1105 86-87 2.295994575278172 1105 88-89 2.1567205531612927 1105 90-91 2.1936330436638265 1105 92-93 2.083730014191133 1105 94-95 2.0517629456682904 1105 96-97 2.3796040595302905 1105 98-99 2.250091101703429 1105 100-101 2.3463917831719723 1106 1 -0.03912173139477915 1106 2 -0.0047650418904083836 1106 3 0.06535802820095427 1106 4 0.09858169427230479 1106 5 0.10857637567589506 1106 6 -0.0559800188322086 1106 7 0.15306609677647032 1106 8 0.0054018520324206065 1106 9 -0.047664511749509586 1106 10-11 0.02842300386672747 1106 12-13 -0.034855589391966646 1106 14-15 -0.0312154921884229 1106 16-17 -0.0014020082866750272 1106 18-19 0.030606618491454185 1106 20-21 0.10494072263693255 1106 22-23 0.09716172177970606 1106 24-25 -0.012850999144184527 1106 26-27 -0.046204181707970804 1106 28-29 0.03842976284634858 1106 30-31 0.12008309627065472 1106 32-33 -0.04853178728280483 1106 34-35 -0.16178521517896627 1106 36-37 0.03816892104047298 1106 38-39 0.16260905674081272 1106 40-41 0.14160470585106566 1106 42-43 0.2847759838815236 1106 44-45 0.2029557919634044 1106 46-47 -0.29072265662883723 1106 48-49 -0.192292933201351 1106 50-51 0.15489204103153753 1106 52-53 0.13437262717923915 1106 54-55 -0.12749631720722476 1106 56-57 0.14724218297504166 1106 58-59 0.3395050556093011 1106 60-61 0.29875124896129535 1106 62-63 0.10830810790228895 1106 64-65 0.41444132116972554 1106 66-67 0.44698362702072103 1106 68-69 0.2864962694579418 1106 70-71 0.29784864824213386 1106 72-73 0.4451565442240799 1106 74-75 0.6957793773301404 1106 76-77 0.7190160458952093 1106 78-79 0.9432746412111648 1106 80-81 0.8018197293601261 1106 82-83 0.9831232469558948 1106 84-85 0.9735165876689926 1106 86-87 1.1129411699653495 1106 88-89 1.2357332978971627 1106 90-91 1.447845187264594 1106 92-93 1.4362979917129337 1106 94-95 0.9861720533688683 1106 96-97 1.2058777635076332 1106 98-99 1.3964197138313565 1106 100-101 1.427936543669448 1107 1 -0.018209281501931684 1107 2 0.08056869118975385 1107 3 0.06042476371290917 1107 4 0.09705058064427163 1107 5 0.0786120197679665 1107 6 9.916768716280444E-4 1107 7 0.04889665409017141 1107 8 -0.0055895200985602855 1107 9 0.1044960643300854 1107 10-11 0.13211538757926178 1107 12-13 0.10478312553303226 1107 14-15 0.16980087799935006 1107 16-17 0.126524610410371 1107 18-19 0.06342021154917887 1107 20-21 0.21323359548412668 1107 22-23 0.13200354418108162 1107 24-25 0.17870226712052073 1107 26-27 0.16638923135265316 1107 28-29 0.20293196700593796 1107 30-31 0.25018646136165756 1107 32-33 0.24594122703035026 1107 34-35 0.33004884547150937 1107 36-37 0.2021322303889974 1107 38-39 0.3176897972718109 1107 40-41 0.3918339356721745 1107 42-43 0.382109894626943 1107 44-45 0.3610143687536578 1107 46-47 0.372062024184423 1107 48-49 0.32323785563238516 1107 50-51 0.38138442918570803 1107 52-53 0.3331010019863001 1107 54-55 0.4062818038716429 1107 56-57 0.44761035257873516 1107 58-59 0.5247360759630695 1107 60-61 0.63698543675347 1107 62-63 0.7562051342300613 1107 64-65 0.8363918636039607 1107 66-67 0.9133268179997671 1107 68-69 0.985617589843919 1107 70-71 0.8544981174671769 1107 72-73 1.2200978314088644 1107 74-75 1.2385677641296127 1107 76-77 1.3500126647032964 1107 78-79 1.463588581271349 1107 80-81 1.65223981053094 1107 82-83 1.7096678936796366 1107 84-85 1.8513463232808824 1107 86-87 2.079124045810648 1107 88-89 2.0070918541448677 1107 90-91 2.1522415695558053 1107 92-93 2.3429543315865686 1107 94-95 2.2485958516299682 1107 96-97 1.9991712470791505 1107 98-99 2.2223117183396113 1107 100-101 1.7204032591940628 1108 1 0.0812993551522645 1108 2 0.08560540191515287 1108 3 0.06907320615277257 1108 4 0.04896213444282438 1108 5 0.03377712912092079 1108 6 0.05198402729978113 1108 7 0.09584929957875232 1108 8 0.0464431262798044 1108 9 0.10258250586392137 1108 10-11 0.19763172135788665 1108 12-13 0.14674015265225648 1108 14-15 0.22989968080652545 1108 16-17 0.15323390923092717 1108 18-19 0.11679738383298144 1108 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-0.21195242320794705 2316 54-55 -0.11520692680377209 2316 56-57 -0.07184638355372641 2316 58-59 -0.3075530399318751 2316 60-61 0.029250745089434815 2316 62-63 -0.2604425550869962 2316 64-65 -0.17681075619670494 2316 66-67 0.138878141005371 2316 68-69 0.24985926051200735 2316 70-71 0.10628828085766884 2316 72-73 0.3385757125703215 2316 74-75 0.5586552922553523 2316 76-77 0.7140785436852433 2316 78-79 0.4735993291642089 2316 80-81 0.07887079721486856 2316 82-83 0.6181160424723195 2316 84-85 0.8047818364225741 2316 86-87 0.8515830585124284 2316 88-89 0.8136066934630897 2316 90-91 1.0809283526406404 2316 92-93 1.0417840070589257 2316 94-95 1.3480687331185237 2316 96-97 1.3707528121268489 2316 98-99 1.3375553260798654 2316 100-101 0.8019252640117269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 37.0 5 84.0 6 208.0 7 489.0 8 1223.0 9 2136.0 10 3500.0 11 5309.0 12 7738.0 13 10983.0 14 14789.0 15 19408.0 16 24293.0 17 30324.0 18 31112.0 19 30438.0 20 32288.0 21 35019.0 22 38313.0 23 42798.0 24 48741.0 25 55105.0 26 63485.0 27 73730.0 28 86705.0 29 101413.0 30 117533.0 31 137366.0 32 160560.0 33 190726.0 34 229293.0 35 277100.0 36 310733.0 37 261580.0 38 79177.0 39 1586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62976406246039 18.626660371619053 14.886546553778295 23.85702901214226 2 16.361117623576675 19.94640152835246 38.49335781316335 25.199123034907515 3 19.52747075203619 23.189089313603432 29.482470229326847 27.800969705033534 4 13.967137916369346 16.405302727313767 35.083960905097214 34.54359845121967 5 14.48836907247194 36.682366227701976 33.56868155366256 15.260583146163519 6 33.99765259517325 34.99470566758879 16.413460038283922 14.594181698954042 7 30.19935517644739 31.749125464986527 19.587564773404946 18.463954585161137 8 27.332218764824724 34.63218051258008 18.86370242129581 19.17189830129939 9 26.628762814626327 16.608238821194952 19.26863622558098 37.494362138597744 10-11 25.776686880430454 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27.446816725987453 22.99497547138989 36-37 23.631233758198242 25.600514787773793 27.48982551664398 23.278425937383986 38-39 23.05652583721446 25.826957196717675 27.609027975794444 23.50748899027342 40-41 23.353750746767243 25.687248631264996 27.72710882416015 23.23189179780761 42-43 23.26124013034078 25.690428165552674 27.4559133606431 23.592418343463446 44-45 23.164381874621085 25.947236014748228 27.639951515074717 23.248430595555973 46-47 23.48030364228183 26.254068759058185 26.990840837273396 23.274786761386583 48-49 23.362855407307208 26.09919765322875 27.006163917600073 23.53178302186397 50-51 23.484284504831425 26.370289647897195 26.95963556619262 23.18579028107876 52-53 23.73189453151296 26.093218265090428 26.879567203174858 23.295320000221754 54-55 23.21887490882423 26.280044445465204 27.042574827488714 23.458505818221848 56-57 23.34414507025187 26.382802804531032 26.963278637111333 23.30977348810577 58-59 23.318287186448412 25.917440093262616 26.92841207250345 23.835860647785527 60-61 22.96075145465446 26.28186598092456 27.296322636696335 23.46105992772464 62-63 23.033177288462316 26.369497675958346 27.147966493251214 23.449358542328127 64-65 22.8037763620391 26.29037549589847 27.023722147567824 23.882125994494604 66-67 22.815711213979956 26.278367579682655 27.04915793183465 23.856763274502736 68-69 23.21865962176346 25.907106195659214 27.233331795577165 23.640902387000164 70-71 23.029672812632903 26.17958280502205 26.842997898898446 23.9477464834466 72-73 23.158330322184657 26.246274012879468 26.639924366545063 23.95547129839081 74-75 22.988292278827977 26.567114473999958 26.514725530245016 23.929867716927053 76-77 23.03531561269721 26.230150213317643 26.84737354386059 23.887160630124555 78-79 23.117977683814708 26.330215867791374 26.938529514022257 23.61327693437166 80-81 23.165567705081912 26.551747419207366 26.64490324762403 23.63778162808669 82-83 23.1135064071088 26.517649248412084 26.618745347124324 23.75009899735479 84-85 23.013804195729428 26.44149863942036 26.689438365633283 23.855258799216937 86-87 23.1400380121307 26.37601913318165 26.50685784536282 23.977085009324835 88-89 22.936596310519526 26.61293005066244 26.231536164210628 24.2189374746074 90-91 22.950554812742904 26.560584704104823 26.441787478495225 24.04707300465705 92-93 23.042010697203104 26.1327120027339 26.538341676127462 24.286935623935538 94-95 22.715911520824918 26.040711349758844 26.684981032257042 24.558396097159193 96-97 22.881435464978605 25.840401022910957 26.71327289531477 24.564890616795665 98-99 22.714982762730752 26.06937990973104 26.297745018298514 24.9178923092397 100-101 22.96573017267767 25.7065877439918 26.56783776923592 24.75984431409461 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 394.0 1 280.0 2 210.5 3 278.0 4 254.0 5 258.5 6 499.0 7 1051.5 8 1611.0 9 1863.5 10 1737.0 11 1502.0 12 1480.0 13 1628.0 14 1784.5 15 1922.5 16 2079.5 17 2268.5 18 2478.0 19 2729.5 20 3103.0 21 3602.5 22 4276.5 23 5157.5 24 6345.0 25 8136.5 26 10314.5 27 12939.0 28 15763.5 29 18648.0 30 22303.0 31 27468.5 32 35106.0 33 38779.5 34 38710.5 35 41656.0 36 46401.5 37 50313.5 38 53976.0 39 57494.5 40 61207.0 41 68035.0 42 73495.5 43 77493.5 44 82536.0 45 89285.5 46 94141.5 47 96645.5 48 97503.5 49 96034.5 50 96537.5 51 96127.0 52 95858.0 53 97973.5 54 99763.5 55 95818.5 56 87814.0 57 81480.5 58 76337.5 59 68023.0 60 55809.0 61 45844.0 62 38896.0 63 30610.5 64 23144.0 65 18117.5 66 13457.5 67 10244.5 68 7938.0 69 5776.0 70 4264.0 71 3127.0 72 2406.5 73 2024.5 74 1601.0 75 1203.5 76 813.5 77 518.5 78 342.0 79 204.5 80 117.0 81 70.0 82 38.0 83 23.5 84 13.0 85 8.5 86 5.5 87 4.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023996749747162958 2 0.010691621174478546 3 0.001504746683815499 4 0.0 5 2.375915816550788E-4 6 0.0 7 0.0 8 0.0 9 1.9799298471256566E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014849473853442425 26-27 0.007880120791560113 28-29 1.3859508929879596E-4 30-31 0.0 32-33 3.959859694251313E-4 34-35 0.009087877998306765 36-37 0.0011681586098041374 38-39 0.011958776276638966 40-41 0.011542991008742579 42-43 0.004573637946860267 44-45 8.315705357927757E-4 46-47 7.919719388502626E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.93978954137697E-5 66-67 4.751831633101576E-4 68-69 6.929754464939799E-4 70-71 0.0 72-73 1.7819368624130908E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3859508929879596E-4 82-83 8.71169132735289E-4 84-85 0.005207215497940477 86-87 0.003781666008010004 88-89 0.0 90-91 0.0012077572067466507 92-93 3.5638737248261816E-4 94-95 7.919719388502626E-5 96-97 0.0 98-99 0.0 100-101 3.959859694251313E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2525342.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.98066554762933 #Duplication Level Percentage of deduplicated Percentage of total 1 73.625688828281 36.06235240211861 2 13.989118424750039 13.703926617377219 3 4.976245109693828 7.312193922028183 4 2.366752288578979 4.63700409123893 5 1.309010729481801 3.2058108369503193 6 0.8075057065535222 2.3731300164300113 7 0.5312394280651654 1.821432252624163 8 0.38696326474520487 1.51629745997629 9 0.2867492953783844 1.2640654199652317 >10 1.5274769033984126 13.393755579741795 >50 0.11823737464814782 3.990407704094788 >100 0.06754398894616534 6.249663436524541 >500 0.00414023403747717 1.406259080977572 >1k 0.003247242382333104 2.8162161231180214 >5k 8.118105955832761E-5 0.24748505683426145 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6224 0.24646166737020175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4969 0.19676542820734774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3443 0.13633796927307273 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3082 0.12204287577682547 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2970 0.11760783291926401 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2939 0.1163802764140461 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2612 0.10343153521384431 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2609 0.10331273942301676 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.959859694251313E-5 0.0 0.0 0.0 0.0 6 3.959859694251313E-5 0.0 0.0 0.0 0.0 7 3.959859694251313E-5 0.0 0.0 0.0 0.0 8 3.959859694251313E-5 0.0 0.0 0.0 0.0 9 3.959859694251313E-5 0.0 0.0 0.0 0.0 10-11 3.959859694251313E-5 0.0 0.0 3.959859694251313E-5 0.0 12-13 7.919719388502626E-5 0.0 0.0 3.959859694251313E-5 0.0 14-15 1.3859508929879596E-4 0.0 0.0 3.959859694251313E-5 0.0 16-17 1.781936862413091E-4 0.0 0.0 3.959859694251313E-5 0.0 18-19 2.1779228318382222E-4 0.0 0.0 7.919719388502626E-5 0.0 20-21 2.375915816550788E-4 0.0 0.0 1.3859508929879596E-4 0.0 22-23 2.375915816550788E-4 1.9799298471256565E-5 0.0 3.959859694251313E-4 1.9799298471256565E-5 24-25 4.3558456636764444E-4 3.959859694251313E-5 0.0 5.93978954137697E-4 3.959859694251313E-5 26-27 4.3558456636764444E-4 3.959859694251313E-5 0.0 9.10767729677802E-4 3.959859694251313E-5 28-29 5.345810587239273E-4 3.959859694251313E-5 0.0 0.002474912308907071 3.959859694251313E-5 30-31 5.543803571951838E-4 3.959859694251313E-5 0.0 0.00916707519219179 3.959859694251313E-5 32-33 5.741796556664404E-4 3.959859694251313E-5 0.0 0.025244105550852124 3.959859694251313E-5 34-35 6.137782526089535E-4 3.959859694251313E-5 0.0 0.05124058444361199 3.959859694251313E-5 36-37 6.335775510802101E-4 3.959859694251313E-5 0.0 0.0936308824705723 3.959859694251313E-5 38-39 7.127747449652364E-4 3.959859694251313E-5 0.0 0.1626908355383152 3.959859694251313E-5 40-41 7.127747449652364E-4 3.959859694251313E-5 0.0 0.2344038946012065 3.959859694251313E-5 42-43 8.117712373215192E-4 3.959859694251313E-5 0.0 0.3095224330011539 3.959859694251313E-5 44-45 9.701656250915718E-4 3.959859694251313E-5 0.0 0.4015099736986119 3.959859694251313E-5 46-47 0.0010295635205053414 3.959859694251313E-5 0.0 0.5136927988367517 3.959859694251313E-5 48-49 0.0010691621174478546 7.919719388502626E-5 0.0 0.6291821068195912 3.959859694251313E-5 50-51 0.0010691621174478546 1.187957908275394E-4 0.0 0.7598772760283558 3.959859694251313E-5 52-53 0.0010691621174478546 1.187957908275394E-4 0.0 0.8858800114994325 3.959859694251313E-5 54-55 0.0010691621174478546 2.375915816550788E-4 0.0 1.026910414510193 3.959859694251313E-5 56-57 0.0010691621174478546 2.375915816550788E-4 0.0 1.1933235181611046 3.959859694251313E-5 58-59 0.0010691621174478546 3.1678877554010504E-4 0.0 1.3676563412005187 3.959859694251313E-5 60-61 0.001088961415919111 3.563873724826182E-4 0.0 1.5601847195350174 3.959859694251313E-5 62-63 0.0011087607143903677 3.563873724826182E-4 0.0 1.7498817981881265 3.959859694251313E-5 64-65 0.0011087607143903677 4.3558456636764444E-4 0.0 1.9547649387686894 3.959859694251313E-5 66-67 0.001187957908275394 4.751831633101576E-4 0.0 2.1896638158316772 3.959859694251313E-5 68-69 0.0013067536991029334 4.751831633101576E-4 0.0 2.4533112742749297 3.959859694251313E-5 70-71 0.0013463522960454465 4.751831633101576E-4 0.0 2.7293926921581315 3.959859694251313E-5 72-73 0.0013859508929879596 4.751831633101576E-4 0.0 3.019492013358983 3.959859694251313E-5 74-75 0.0014057501914592162 5.147817602526707E-4 0.0 3.319708776078646 3.959859694251313E-5 76-77 0.0014255494899304728 5.147817602526707E-4 0.0 3.643902489247001 3.959859694251313E-5 78-79 0.0014453487884017293 5.147817602526707E-4 0.0 4.003200358604894 3.959859694251313E-5 80-81 0.0016631410715855516 5.147817602526707E-4 0.0 4.379901811319021 3.959859694251313E-5 82-83 0.001682940370056808 5.147817602526707E-4 0.0 4.7698489947104195 3.959859694251313E-5 84-85 0.0017423382654705778 5.147817602526707E-4 0.0 5.170784788753364 3.959859694251313E-5 86-87 0.0018017361608843475 5.543803571951838E-4 0.0 5.586233468575742 3.959859694251313E-5 88-89 0.0018215354593556041 5.741796556664404E-4 0.0 6.016016840491307 3.959859694251313E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3945 0.0 50.466038 1 GTATCAA 6245 0.0 43.596783 1 ATCAACG 8390 0.0 32.212746 3 TATCAAC 8490 0.0 32.060284 2 TCAACGC 8600 0.0 31.426151 4 CAACGCA 8715 0.0 30.956963 5 AACGCAG 9160 0.0 29.400612 6 GTGGTAT 1590 0.0 26.2977 1 ACGCAGA 10710 0.0 24.92388 7 TAAGGTG 1315 0.0 24.923025 5 CGCAGAG 10740 0.0 24.721588 8 CGTTACG 50 0.0016542077 23.750525 42-43 GTACATG 8555 0.0 23.382683 1 TGGTATC 1825 0.0 22.124653 2 TACATGG 8815 0.0 22.094387 2 ACATGGG 8695 0.0 21.14067 3 GCAGAGT 12145 0.0 21.04038 9 AGGTAAG 1395 0.0 20.77189 2 GAGTACT 6945 0.0 20.4146 12-13 GGTAAGG 1505 0.0 20.198582 3 >>END_MODULE