FastQCFastQC Report
Thu 26 May 2016
SRR938599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3981538
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71370.1792523391714458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53130.13344089645760004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58000.039.76721
GTATCAA91800.038.4908371
ATCAACG114800.030.2411063
TCAACGC117650.029.589384
TATCAAC118050.029.5696932
CAACGCA119300.029.060555
AACGCAG125200.027.821676
GTACATG115350.024.943021
TACATGG117050.023.9716152
ACGCAGA143500.023.9100117
CGCAGAG146500.023.2257588
ACATGGG123850.021.9642683
GCAGAGT159250.020.7089529
GAGTACT79550.020.1290912-13
CATGGGG84800.019.3481714
GGACCGA13550.019.2888766
GTACTTT84500.018.7786414-15
AGTACTT85000.018.58690812-13
AGAGTAC143900.018.55872510-11
GTATAGG7800.018.291021
Produced by FastQC (version 0.11.5)