##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938599_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3981538 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.18562023017236 31.0 31.0 34.0 28.0 34.0 2 31.22649488715165 31.0 31.0 34.0 28.0 34.0 3 31.285296285003433 31.0 31.0 34.0 27.0 34.0 4 34.93049168436921 37.0 35.0 37.0 32.0 37.0 5 34.90026291347716 37.0 35.0 37.0 32.0 37.0 6 34.913182041713526 37.0 35.0 37.0 32.0 37.0 7 34.8226670196291 37.0 35.0 37.0 32.0 37.0 8 34.79539037427246 37.0 35.0 37.0 32.0 37.0 9 36.29981554866486 39.0 35.0 39.0 32.0 39.0 10-11 36.13126234133644 39.0 35.0 39.0 31.0 39.0 12-13 35.99769674432342 38.0 35.0 39.0 30.0 39.0 14-15 36.99146548394113 39.0 36.0 41.0 31.0 41.0 16-17 36.819078958934966 39.0 36.0 41.0 30.5 41.0 18-19 36.947971864138935 39.0 36.0 41.0 31.0 41.0 20-21 36.916899198249524 39.0 36.0 41.0 30.0 41.0 22-23 36.83178108560059 39.0 36.0 41.0 30.0 41.0 24-25 36.7051815152838 39.0 36.0 41.0 30.0 41.0 26-27 36.503093653758924 39.0 36.0 41.0 30.0 41.0 28-29 36.30056689148766 39.0 35.0 40.5 29.0 41.0 30-31 36.07842471929189 39.0 35.0 40.0 28.5 41.0 32-33 35.84728966545089 38.0 35.0 40.0 27.5 41.0 34-35 35.60874692141579 38.0 34.5 40.0 27.0 41.0 36-37 35.3537553829701 38.0 34.0 40.0 26.5 41.0 38-39 35.26138090356038 38.0 34.0 40.0 26.0 41.0 40-41 35.18801792171769 38.0 34.0 40.0 25.5 41.0 42-43 34.92573309610508 38.0 34.0 40.0 25.0 41.0 44-45 34.622861316405874 38.0 33.0 40.0 24.0 41.0 46-47 34.28513893374872 38.0 33.0 40.0 23.5 41.0 48-49 33.96979835430429 38.0 33.0 40.0 23.0 41.0 50-51 33.288233340985315 37.5 32.0 39.5 22.0 40.5 52-53 33.32459542016176 37.0 32.0 39.5 22.0 40.0 54-55 33.44180439819989 37.0 32.0 40.0 21.5 41.0 56-57 33.12527709141543 37.0 32.0 40.0 19.5 41.0 58-59 32.762153218178504 36.5 31.0 39.5 18.0 41.0 60-61 32.285441706194945 36.0 31.0 39.0 14.0 40.5 62-63 31.715229265675728 35.5 30.5 39.0 10.0 40.0 64-65 31.197722211868882 35.0 29.5 38.5 8.0 40.0 66-67 30.661691034971913 35.0 29.0 38.0 6.5 40.0 68-69 30.078608442265278 34.0 28.5 37.5 2.0 40.0 70-71 29.44975069935286 34.0 28.0 37.0 2.0 39.0 72-73 28.807767877639243 34.0 26.5 36.0 2.0 39.0 74-75 28.121300612979205 33.5 26.0 36.0 2.0 38.5 76-77 27.36852517795887 33.0 25.5 35.0 2.0 37.5 78-79 26.58918224565482 32.0 23.5 35.0 2.0 37.0 80-81 25.89340551314592 31.5 21.5 35.0 2.0 36.0 82-83 25.164682843664934 31.0 19.0 34.5 2.0 36.0 84-85 24.46372658003013 31.0 16.5 34.0 2.0 35.0 86-87 23.693190294805675 30.5 8.5 34.0 2.0 35.0 88-89 22.885057357232306 29.5 2.0 34.0 2.0 35.0 90-91 21.967279352853094 29.0 2.0 34.0 2.0 35.0 92-93 21.001131221151223 29.0 2.0 34.0 2.0 35.0 94-95 19.980532397279646 27.0 2.0 33.5 2.0 35.0 96-97 18.707950294584656 25.0 2.0 33.0 2.0 34.5 98-99 17.15755934013439 21.0 2.0 32.0 2.0 34.0 100-101 13.577837885761733 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4250466305474241 1101 2 0.33909785955470895 1101 3 0.35174318755742107 1101 4 0.22644335523963832 1101 5 0.22849842429199185 1101 6 0.4378403690137347 1101 7 0.3752812792043514 1101 8 0.49812605360671114 1101 9 0.6066727817081699 1101 10-11 0.5781764091164021 1101 12-13 0.5578774962493682 1101 14-15 0.7735921977367255 1101 16-17 0.6110694138210917 1101 18-19 0.7914662753465223 1101 20-21 0.7511491147754512 1101 22-23 0.7944574680856391 1101 24-25 0.7421810474574073 1101 26-27 0.6978337718378924 1101 28-29 0.7544054338222352 1101 30-31 0.7633278739254905 1101 32-33 0.8424977214513802 1101 34-35 0.6749210020776673 1101 36-37 0.7082359758045982 1101 38-39 0.6994019878079243 1101 40-41 0.8040640190755752 1101 42-43 0.8306189803277846 1101 44-45 0.8203744742576831 1101 46-47 0.735102621477516 1101 48-49 0.5773723536435114 1101 50-51 0.8336152774930525 1101 52-53 0.8034898542851963 1101 54-55 0.8082953597816029 1101 56-57 0.8160966447277787 1101 58-59 0.9563527992919418 1101 60-61 0.9679672438146412 1101 62-63 0.8050446544338747 1101 64-65 0.7100960748581358 1101 66-67 0.8165136013262284 1101 68-69 0.6955021054646551 1101 70-71 0.8338019022910146 1101 72-73 0.7374744114444134 1101 74-75 0.7612614980657106 1101 76-77 0.9116038118974998 1101 78-79 1.202439957226968 1101 80-81 1.1178027549796 1101 82-83 0.9859487196103736 1101 84-85 1.2024910015687098 1101 86-87 1.3106768121906285 1101 88-89 1.264648412604597 1101 90-91 1.061158915019604 1101 92-93 1.162126973808057 1101 94-95 1.01207347922238 1101 96-97 1.0419838461129132 1101 98-99 1.188576693801803 1101 100-101 1.0450045383654203 1102 1 0.5222524973859279 1102 2 0.46904971532445927 1102 3 0.4571437669419396 1102 4 0.4109375485636022 1102 5 0.3848963177502469 1102 6 0.4034786186566137 1102 7 0.3390487184787574 1102 8 0.4251740010971403 1102 9 0.6475516812197384 1102 10-11 0.7184346158757009 1102 12-13 0.6929732429625304 1102 14-15 0.7808475845585789 1102 16-17 0.8069603672108556 1102 18-19 0.7085415810773554 1102 20-21 0.7837265204900277 1102 22-23 0.8341912491683914 1102 24-25 0.761649807169654 1102 26-27 0.6524802531889549 1102 28-29 0.5152792295036548 1102 30-31 0.7231736327566836 1102 32-33 0.7190731725986055 1102 34-35 0.5233023180674863 1102 36-37 0.8614379520418467 1102 38-39 0.7056982323583725 1102 40-41 0.763992679505499 1102 42-43 0.7902903464149631 1102 44-45 0.7659289027570182 1102 46-47 0.8532672670464478 1102 48-49 0.8910995182805266 1102 50-51 0.9923673126096872 1102 52-53 0.7967123099973534 1102 54-55 0.7850059269910616 1102 56-57 0.8757567838142748 1102 58-59 0.8367366985611611 1102 60-61 0.9345755775994249 1102 62-63 0.9700451747964429 1102 64-65 1.003368606557487 1102 66-67 0.8798761201091807 1102 68-69 1.0116840983535091 1102 70-71 1.1278177327183947 1102 72-73 1.0645875824626394 1102 74-75 1.1429239923274679 1102 76-77 1.2602324783151495 1102 78-79 1.279765942280445 1102 80-81 1.4591963501004521 1102 82-83 1.4746754565521236 1102 84-85 1.4747928503527703 1102 86-87 1.6604025882436844 1102 88-89 1.5342731741644862 1102 90-91 1.5399896032953393 1102 92-93 1.5282991653471036 1102 94-95 1.525022773097497 1102 96-97 1.5928728463609794 1102 98-99 1.5472931232876839 1102 100-101 1.500058934074783 1103 1 0.36209570782180833 1103 2 0.2730532293113441 1103 3 0.3345792471591871 1103 4 0.27147343156330805 1103 5 0.18124316648858496 1103 6 0.31222062792779326 1103 7 0.24260504172134745 1103 8 0.3309881023507941 1103 9 0.3801363124997508 1103 10-11 0.437655115790065 1103 12-13 0.4968876986537012 1103 14-15 0.6285725059635112 1103 16-17 0.6429058354938135 1103 18-19 0.6518565456336063 1103 20-21 0.4778891100663074 1103 22-23 0.6886011369528688 1103 24-25 0.796854872614901 1103 26-27 0.6172649317088883 1103 28-29 0.6916560935466194 1103 30-31 0.7424990757345142 1103 32-33 0.7400585738064009 1103 34-35 0.7860959445461475 1103 36-37 0.9226019740097939 1103 38-39 0.8162068004639664 1103 40-41 0.7896825273432313 1103 42-43 0.801749652402556 1103 44-45 0.8125988338585657 1103 46-47 0.5720687889453302 1103 48-49 0.8283613848926876 1103 50-51 1.0777210732939224 1103 52-53 0.9849915269197353 1103 54-55 0.9661188690710603 1103 56-57 0.9263944536431694 1103 58-59 0.8889456827095401 1103 60-61 1.012958169827776 1103 62-63 0.9969975163493352 1103 64-65 1.1943598713165073 1103 66-67 1.1396552665860753 1103 68-69 1.1495396408943002 1103 70-71 1.255298588120045 1103 72-73 1.2918011593808316 1103 74-75 1.302852455621224 1103 76-77 1.332568775423848 1103 78-79 1.1916036510615449 1103 80-81 1.4005876692820713 1103 82-83 1.362754076483501 1103 84-85 1.3799685220712554 1103 86-87 1.4786574669393104 1103 88-89 1.399031159368171 1103 90-91 1.3777824700677215 1103 92-93 1.335598112532324 1103 94-95 1.3982993448607353 1103 96-97 1.518030519946958 1103 98-99 1.5282608924032743 1103 100-101 1.526892105337442 1104 1 0.38165360350054556 1104 2 0.3179586925891442 1104 3 0.37178162191831987 1104 4 0.2987190688902288 1104 5 0.3332742077451343 1104 6 0.43452582151685704 1104 7 0.3665653562749682 1104 8 0.49644193272157366 1104 9 0.5916021415220882 1104 10-11 0.693037185234914 1104 12-13 0.6760768480521051 1104 14-15 0.7428220231873865 1104 16-17 0.806204471026966 1104 18-19 0.7806203005914227 1104 20-21 0.7681500640902001 1104 22-23 0.7242136799761312 1104 24-25 0.6658336970550209 1104 26-27 0.7646431399851679 1104 28-29 0.7331368636577835 1104 30-31 0.8849644646398218 1104 32-33 0.7526002184498708 1104 34-35 0.62720290130855 1104 36-37 0.7379752203046976 1104 38-39 0.9176539063043094 1104 40-41 0.9013043481999929 1104 42-43 0.9201194141775133 1104 44-45 0.7111432742336277 1104 46-47 0.8109218887010243 1104 48-49 0.6847466714577735 1104 50-51 0.8762986004812916 1104 52-53 0.8873358784390248 1104 54-55 1.0980876488543885 1104 56-57 1.1228788757892758 1104 58-59 0.9923894424118842 1104 60-61 1.0151258502549467 1104 62-63 1.0865786300223057 1104 64-65 1.1265766461222455 1104 66-67 1.2187257248488308 1104 68-69 1.4196531453079544 1104 70-71 1.3594567576493795 1104 72-73 1.3116259345361705 1104 74-75 1.2470405697561588 1104 76-77 1.2953189294127831 1104 78-79 1.3925231887201655 1104 80-81 1.3418446612122175 1104 82-83 1.3778291252813482 1104 84-85 1.5294699308619428 1104 86-87 1.594475746466685 1104 88-89 1.6812551654111978 1104 90-91 1.5942559309066695 1104 92-93 1.6703766962086632 1104 94-95 1.6519061195416214 1104 96-97 1.834231977015257 1104 98-99 1.9728110886351224 1104 100-101 1.7789269963174341 1105 1 0.3961490909449559 1105 2 0.35440568394376015 1105 3 0.24892982321260249 1105 4 0.32485018751002315 1105 5 0.3032961074732867 1105 6 0.4450436258505519 1105 7 0.34339025769170917 1105 8 0.3885588062983558 1105 9 0.49977614795089664 1105 10-11 0.5421321622848936 1105 12-13 0.5517251223618942 1105 14-15 0.6601069925073304 1105 16-17 0.6061560382254996 1105 18-19 0.630873275327076 1105 20-21 0.6534012940875868 1105 22-23 0.6969477734916367 1105 24-25 0.7489903299785894 1105 26-27 0.6231192865687945 1105 28-29 0.6231344404785943 1105 30-31 0.7928537352013194 1105 32-33 0.7716105701531575 1105 34-35 0.7894868929840442 1105 36-37 0.8498701365781898 1105 38-39 0.7451976411178904 1105 40-41 0.8376268191729466 1105 42-43 0.9273824566809097 1105 44-45 1.0474496707125311 1105 46-47 0.8665998789694029 1105 48-49 0.9169750592974495 1105 50-51 0.9138533220976512 1105 52-53 0.8889752490428577 1105 54-55 1.0063807826136326 1105 56-57 1.105880821720362 1105 58-59 1.110753403679432 1105 60-61 1.0206248848002701 1105 62-63 1.088773620345595 1105 64-65 1.1040230412861405 1105 66-67 1.2770321503861837 1105 68-69 1.1711344490276083 1105 70-71 1.167610195374781 1105 72-73 1.0919778237417255 1105 74-75 1.1632098669923288 1105 76-77 1.1850788980001106 1105 78-79 1.2346224671594612 1105 80-81 1.3225516668350288 1105 82-83 1.1825892194602758 1105 84-85 1.3054383960414242 1105 86-87 1.3780813816880908 1105 88-89 1.260902436465706 1105 90-91 1.2790070292791214 1105 92-93 1.283137055607508 1105 94-95 1.3672686631015942 1105 96-97 1.6865329568021536 1105 98-99 1.7089202262616077 1105 100-101 1.6516149410748593 1106 1 0.23159078241948805 1106 2 0.14170336603707057 1106 3 -0.030416893192768413 1106 4 0.11053045914290038 1106 5 0.16373660406313206 1106 6 0.007723702848984715 1106 7 0.2381913371042046 1106 8 0.2375733580857471 1106 9 0.15269567378430793 1106 10-11 0.17014535405422748 1106 12-13 0.19745825726865007 1106 14-15 0.43034211492076224 1106 16-17 0.22601166586586885 1106 18-19 0.01575541850375828 1106 20-21 0.20667297924573091 1106 22-23 0.23876785941551049 1106 24-25 0.12204711277714608 1106 26-27 0.06267877786351761 1106 28-29 0.1960108668665228 1106 30-31 -0.14318710083875175 1106 32-33 0.17606401596133026 1106 34-35 -0.21225842617253932 1106 36-37 -0.04671260829336177 1106 38-39 -0.07499332356689337 1106 40-41 0.0840279445998533 1106 42-43 -0.3740842862403042 1106 44-45 -0.052363969679213085 1106 46-47 0.039555637220210826 1106 48-49 0.1928998319013573 1106 50-51 -0.07784596991877635 1106 52-53 -0.05543268755998554 1106 54-55 0.0014956275400166419 1106 56-57 -0.7805627283223444 1106 58-59 -0.3866579823692504 1106 60-61 0.3050370036509875 1106 62-63 0.29051213668589426 1106 64-65 0.25553426905272403 1106 66-67 -0.14455975829687873 1106 68-69 -0.004155165882711032 1106 70-71 -0.1644991156453095 1106 72-73 0.25120376623011964 1106 74-75 0.5700220666772218 1106 76-77 0.49625070945560523 1106 78-79 0.6554484683834829 1106 80-81 0.7654808238328208 1106 82-83 0.5801809376887164 1106 84-85 0.5175652308254897 1106 86-87 0.46866675645693334 1106 88-89 0.46153039153367814 1106 90-91 0.6190942628281633 1106 92-93 0.8354693402894355 1106 94-95 0.6054805425392118 1106 96-97 0.5911801284025557 1106 98-99 0.8920610665481377 1106 100-101 0.3719511031803382 1107 1 0.25427575260309965 1107 2 0.1456348569375301 1107 3 0.24411173383388984 1107 4 0.17356445921299013 1107 5 0.20256456752301943 1107 6 0.37818060218052096 1107 7 0.2595008842483679 1107 8 0.25309274406590987 1107 9 0.45484670509622305 1107 10-11 0.46196477456965823 1107 12-13 0.42228982077947563 1107 14-15 0.5991483481055084 1107 16-17 0.7290357473923521 1107 18-19 0.5950589472475514 1107 20-21 0.6625814250300337 1107 22-23 0.5295722670387661 1107 24-25 0.71883462823304 1107 26-27 0.5803581591802711 1107 28-29 0.6393982960634901 1107 30-31 0.7328436959573708 1107 32-33 0.771045782360261 1107 34-35 0.6906835526367985 1107 36-37 0.6215270773987029 1107 38-39 0.6894091255666623 1107 40-41 0.8705641380382261 1107 42-43 0.9216105339799299 1107 44-45 0.8718109482048675 1107 46-47 0.8324735470068916 1107 48-49 0.9102893177167957 1107 50-51 0.8075773284090673 1107 52-53 0.7896901873806073 1107 54-55 0.9238080887846962 1107 56-57 1.0599403278112902 1107 58-59 0.8716513955327301 1107 60-61 0.955624869320225 1107 62-63 1.1836581447978993 1107 64-65 1.133588223323514 1107 66-67 1.0920756998273013 1107 68-69 1.1494635615674405 1107 70-71 1.2748559113321782 1107 72-73 1.4174054131098934 1107 74-75 1.3232112958292142 1107 76-77 1.3623532765655924 1107 78-79 1.4847531858731777 1107 80-81 1.5437038970210004 1107 82-83 1.3746294238692371 1107 84-85 1.5816993051613828 1107 86-87 1.5774208045770166 1107 88-89 1.6389792477223715 1107 90-91 1.6677345752622905 1107 92-93 1.772093069454023 1107 94-95 1.8417338408688195 1107 96-97 1.9445683095913822 1107 98-99 1.8256811430047541 1107 100-101 1.5884705150487406 1108 1 0.2894292574457431 1108 2 0.23164350153732727 1108 3 0.21155612991300288 1108 4 0.07265826047026991 1108 5 0.19691758543063997 1108 6 0.18270779207284704 1108 7 0.2627472707185632 1108 8 0.2511083312419444 1108 9 0.2576139822044894 1108 10-11 0.37895429271843994 1108 12-13 0.3476550320857612 1108 14-15 0.4073766044619518 1108 16-17 0.43797012608232677 1108 18-19 0.45203770568831203 1108 20-21 0.3944026160552383 1108 22-23 0.37148643022591443 1108 24-25 0.4862348873633522 1108 26-27 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1.1100164957568452 2205 46-47 1.0864111407565318 2205 48-49 1.1462099620387391 2205 50-51 1.0243414082884286 2205 52-53 1.1708652694979946 2205 54-55 1.0954587055313922 2205 56-57 1.1269873432802697 2205 58-59 1.25506905007812 2205 60-61 1.2052964783044118 2205 62-63 1.3210267469335726 2205 64-65 1.5013671339957497 2205 66-67 1.506322833857439 2205 68-69 1.6473044288234568 2205 70-71 1.7839298896891798 2205 72-73 1.6699494070324086 2205 74-75 1.7555190192891565 2205 76-77 1.8823536640888143 2205 78-79 2.1475140723517434 2205 80-81 2.0434483411686912 2205 82-83 2.3014565740252344 2205 84-85 2.4709800812691434 2205 86-87 2.476317131467866 2205 88-89 2.4225517195392214 2205 90-91 2.4759014176236036 2205 92-93 2.7612534166119858 2205 94-95 2.9650729678488332 2205 96-97 2.944168567172131 2205 98-99 3.0866859732547454 2205 100-101 2.5752838156136697 2206 1 0.2786568983975215 2206 2 0.28408323328740437 2206 3 0.27537236959131306 2206 4 0.2621485465122859 2206 5 0.2663562548693079 2206 6 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0.6314127484416794 2206 70-71 0.6263188303539806 2206 72-73 1.0024818561092452 2206 74-75 1.1539829571147955 2206 76-77 1.3873520927849654 2206 78-79 1.5725994124774338 2206 80-81 1.5508572641315403 2206 82-83 1.3571877170271023 2206 84-85 1.5409083000234816 2206 86-87 1.5342844231108685 2206 88-89 1.2621240004174439 2206 90-91 1.4323807476172412 2206 92-93 1.5930487048636195 2206 94-95 1.4917271181640288 2206 96-97 1.801921224117745 2206 98-99 2.119876116642981 2206 100-101 1.8283172432252321 2207 1 0.283446135959867 2207 2 0.3045087990911206 2207 3 0.39209532412939296 2207 4 0.2778715924874291 2207 5 0.44325276285825055 2207 6 0.3512393474487965 2207 7 0.4635121390000947 2207 8 0.2472244724174928 2207 9 0.35094036025993347 2207 10-11 0.3926358010102078 2207 12-13 0.4476868461135126 2207 14-15 0.6487001317026042 2207 16-17 0.5736555939034034 2207 18-19 0.5722020386025761 2207 20-21 0.5865857733789355 2207 22-23 0.5294603021957229 2207 24-25 0.631656345071093 2207 26-27 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12621.0 10 15884.0 11 19687.0 12 23544.0 13 27222.0 14 30791.0 15 35255.0 16 39773.0 17 45375.0 18 45886.0 19 45941.0 20 49873.0 21 54932.0 22 61527.0 23 68744.0 24 76951.0 25 85915.0 26 96596.0 27 110014.0 28 125842.0 29 143706.0 30 165989.0 31 192657.0 32 227236.0 33 270778.0 34 328366.0 35 400906.0 36 473206.0 37 453208.0 38 193438.0 39 7655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.617410865439396 18.15007419716855 13.970869699323133 24.261645238068922 2 15.78490705790687 19.46827161372254 39.74881385156871 24.998007476801874 3 19.65055005125732 22.95012840103063 28.748554498335803 28.650767049376253 4 13.356279346249853 15.906148326158934 35.91605058348637 34.82152174410485 5 14.878545692744737 36.32749483621409 33.54814414377248 15.245815327268689 6 33.77335949908968 34.74243272147498 17.016465219505253 14.467742559930091 7 30.296774050917683 31.13325124990485 19.8580681888696 18.71190651030787 8 26.684059791483485 34.946263032282374 19.334882552443162 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26.231853175469706 27.326498729882477 23.427756836006456 34-35 23.628037344893148 26.19119812247656 27.12680583900038 23.05395869362991 36-37 23.584505017771274 25.943520923462906 27.083111359944677 23.388862698821143 38-39 23.11844111861196 26.28621281363921 27.285624916777422 23.30972115097141 40-41 23.4131789785975 26.040954791040217 27.26455593030922 23.281310300053065 42-43 23.292755924404293 25.982183291135424 27.198613655791938 23.526447128668345 44-45 23.335639137177246 26.241969246906383 27.09187648728116 23.33051512863522 46-47 23.344486704813615 26.48303815477912 26.73865177630241 23.43382336410485 48-49 23.404075216763243 26.27290554299509 26.874397007726593 23.448622232515078 50-51 23.388972412884527 26.430750137300723 26.807292580461112 23.372984869353637 52-53 23.570480806847733 26.35329164963337 26.668048169226527 23.408179374292374 54-55 23.24451492746792 26.27195671146709 26.901619288435448 23.58190907262954 56-57 23.213118106110294 26.490233161708538 26.96873451225844 23.327914219922725 58-59 23.294205864786974 26.171874067789265 26.882737545350583 23.65118252207318 60-61 23.309976228383373 26.218290641770842 27.08239675676015 23.38933637308563 62-63 23.17704781403985 26.39052264556301 26.913818780737337 23.518610759659804 64-65 23.0158483961397 26.178846207222133 26.95012284638908 23.85518255024909 66-67 22.9355855519812 26.31392608299982 26.91268658909594 23.837801775923033 68-69 23.024471383404432 26.069597867322564 27.11417123764066 23.79175951163234 70-71 22.997763009666492 26.15257510147354 26.909895818154595 23.939766070705375 72-73 23.211945702539715 26.115415767688226 26.74712921473082 23.92550931504124 74-75 22.976972806142093 26.342072189935568 26.77037058438186 23.910584419540488 76-77 23.164467366599066 26.062908502376196 26.794402131827372 23.97822199919736 78-79 23.138200142715444 26.1919389634016 26.822227842874362 23.84763305100859 80-81 23.020591241218753 26.343142645226386 26.814703443779038 23.82156266977583 82-83 23.13592284341836 26.281636442710454 26.636720885468122 23.945719828403064 84-85 23.09178278019677 26.021587452768735 26.791478900134262 24.09515086690023 86-87 23.14127061717256 25.96851917068615 26.83619750285479 24.0540127092865 88-89 23.036447556379713 25.998567619790364 26.816878569408697 24.14810625442123 90-91 23.003347241964324 26.0477985398764 26.83819737609897 24.1106568420603 92-93 23.152372795724236 25.751628798089836 26.818785802091206 24.277212604094725 94-95 22.967537462838063 25.685046688697582 26.91004785398335 24.437367994481004 96-97 23.116125435098823 25.60402508340153 26.811798857392112 24.468050624107534 98-99 23.03060365851131 25.59495808811513 26.61356014936114 24.76087810401242 100-101 23.583837679055403 25.030393956447348 26.763609794130428 24.622158570366818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 352.0 1 258.5 2 219.0 3 294.5 4 279.0 5 277.5 6 510.0 7 1082.5 8 1760.0 9 2033.5 10 1834.5 11 1631.5 12 1679.5 13 1895.0 14 2109.5 15 2271.0 16 2471.5 17 2744.0 18 2954.5 19 3216.0 20 3667.0 21 4236.0 22 5080.0 23 6174.0 24 7614.0 25 9836.5 26 12757.0 27 16645.0 28 20937.0 29 25806.5 30 31632.0 31 38091.0 32 45227.0 33 51849.5 34 57936.5 35 64600.5 36 73151.0 37 82345.0 38 90684.0 39 97225.5 40 104311.0 41 115753.0 42 127146.5 43 137033.0 44 145237.5 45 151669.0 46 155771.5 47 160129.5 48 165875.0 49 166955.5 50 164608.0 51 160053.5 52 154400.5 53 152011.0 54 151402.0 55 142807.0 56 129077.5 57 118735.5 58 109262.5 59 96667.0 60 81647.5 61 68104.5 62 56426.5 63 44666.5 64 34511.5 65 26972.0 66 20509.5 67 15788.0 68 12171.5 69 8923.5 70 6694.5 71 5213.5 72 4207.0 73 3428.0 74 2590.0 75 1948.0 76 1341.0 77 824.0 78 536.5 79 342.5 80 202.0 81 119.0 82 83.0 83 55.0 84 33.5 85 22.5 86 20.0 87 17.5 88 11.5 89 8.0 90 5.5 91 4.5 92 3.5 93 1.0 94 1.0 95 2.0 96 2.0 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006379444325283345 2 0.1047082810712845 3 0.1141518679465071 4 0.11721601049644635 5 0.15715032733581846 6 0.09594282410465503 7 0.025994979829402607 8 0.026070327597024063 9 0.022051779990546368 10-11 0.05106067052480724 12-13 0.052944364715343674 14-15 0.02707496449864349 16-17 0.031457692981958225 18-19 0.03635529787735292 20-21 0.0585577734031422 22-23 0.031608388517201144 24-25 0.04962906293999957 26-27 0.0570633760119833 28-29 0.05353458889504508 30-31 0.0678004328980409 32-33 0.049855106242863934 34-35 0.061948422946107765 36-37 0.052291350729291045 38-39 0.03134467133052604 40-41 0.03621716030338025 42-43 0.032286518425794254 44-45 0.007032458311335971 46-47 0.01329888098518713 48-49 0.00881568881171045 50-51 0.00787384171644224 52-53 0.00917986968854749 54-55 0.010988216111462454 56-57 0.01424072808045534 58-59 0.018723920253932023 60-61 0.023872684374731574 62-63 0.010724498924787357 64-65 0.008702667160278264 66-67 0.009971021248572787 68-69 0.011025889995273184 70-71 0.029887947823177878 72-73 0.027476819259291258 74-75 0.02851913004472141 76-77 0.022993627085814577 78-79 0.0047469093601517805 80-81 0.004646445669989838 82-83 0.005814336068122419 84-85 0.004960394701745908 86-87 0.012859352340728632 88-89 0.036405529722433896 90-91 0.03504926990524767 92-93 0.028858194999017966 94-95 0.04589934844273746 96-97 0.04257148870612311 98-99 0.024726625741108083 100-101 0.07411708741697304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3981538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.18483845978648 #Duplication Level Percentage of deduplicated Percentage of total 1 72.04879352490161 33.27561890170091 2 13.811152873716217 12.757317288319985 3 5.473113935646424 7.583246489695089 4 2.651012153904753 4.8974627233208645 5 1.5381677777303473 3.552001516926242 6 0.9398774016525373 2.6044851580395774 7 0.6246699807539988 2.0195197506260953 8 0.4750314816911015 1.755140179617323 9 0.3509753848795321 1.4588767308620318 >10 1.8580736053248799 15.578342576124351 >50 0.13825821146944223 4.403099385689774 >100 0.08421226548909969 7.1922347486015115 >500 0.005023352999521815 1.517020560461214 >1k 0.0015288465178986146 1.0916737608136218 >5k 1.0920332270704367E-4 0.31396022920142236 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7137 0.1792523391714458 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5313 0.13344089645760004 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.5115922540485613E-5 0.0 4 0.0 0.0 0.0 2.5115922540485613E-5 0.0 5 0.0 0.0 0.0 2.5115922540485613E-5 0.0 6 0.0 0.0 0.0 5.0231845080971226E-5 0.0 7 0.0 0.0 0.0 5.0231845080971226E-5 0.0 8 0.0 0.0 0.0 5.0231845080971226E-5 0.0 9 0.0 0.0 0.0 7.534776762145683E-5 0.0 10-11 0.0 0.0 0.0 2.009273803238849E-4 0.0 12-13 0.0 0.0 0.0 2.511592254048561E-4 0.0 14-15 2.5115922540485613E-5 0.0 0.0 2.762751479453417E-4 0.0 16-17 2.5115922540485613E-5 0.0 0.0 3.2650699302631293E-4 0.0 18-19 3.767388381072842E-5 0.0 0.0 3.5162291556679854E-4 0.0 20-21 5.0231845080971226E-5 0.0 0.0 4.52086605728741E-4 0.0 22-23 5.0231845080971226E-5 0.0 0.0 6.027821409716547E-4 0.0 24-25 5.0231845080971226E-5 0.0 0.0 7.409197149443256E-4 0.0 26-27 5.0231845080971226E-5 0.0 0.0 0.001230680204483795 0.0 28-29 5.0231845080971226E-5 0.0 0.0 0.003039026627398759 0.0 30-31 7.534776762145683E-5 0.0 0.0 0.0103854339704908 0.0 32-33 7.534776762145683E-5 0.0 0.0 0.026936826924670816 0.0 34-35 7.534776762145683E-5 0.0 0.0 0.052818785102641236 0.0 36-37 1.0046369016194245E-4 0.0 0.0 0.09817814121075825 0.0 38-39 1.0046369016194245E-4 0.0 0.0 0.16723437023582344 0.0 40-41 1.0046369016194245E-4 0.0 0.0 0.24280818116014466 0.0 42-43 1.1302165143218525E-4 0.0 0.0 0.32374424154685955 0.0 44-45 1.6325349651315647E-4 0.0 0.0 0.42108099935251153 1.2557961270242806E-5 46-47 2.009273803238849E-4 0.0 0.0 0.5379328289721208 2.5115922540485613E-5 48-49 2.009273803238849E-4 0.0 0.0 0.6653207880974639 2.5115922540485613E-5 50-51 2.009273803238849E-4 0.0 0.0 0.8013109506929232 2.5115922540485613E-5 52-53 2.009273803238849E-4 0.0 0.0 0.9379792431969756 2.5115922540485613E-5 54-55 2.260433028643705E-4 0.0 0.0 1.0902821974824803 2.5115922540485613E-5 56-57 2.511592254048561E-4 0.0 0.0 1.2690322182031164 2.5115922540485613E-5 58-59 2.511592254048561E-4 0.0 0.0 1.4628141185642332 2.5115922540485613E-5 60-61 2.511592254048561E-4 0.0 0.0 1.6717283622559926 2.5115922540485613E-5 62-63 2.511592254048561E-4 0.0 0.0 1.8835309370399078 2.5115922540485613E-5 64-65 2.511592254048561E-4 2.5115922540485613E-5 0.0 2.112563034686596 2.5115922540485613E-5 66-67 3.0139107048582733E-4 2.5115922540485613E-5 0.0 2.370264957913249 2.5115922540485613E-5 68-69 3.0139107048582733E-4 2.5115922540485613E-5 0.0 2.651588406289228 2.5115922540485613E-5 70-71 3.0139107048582733E-4 2.5115922540485613E-5 0.0 2.9506060220949797 5.0231845080971226E-5 72-73 3.0139107048582733E-4 2.5115922540485613E-5 0.0 3.2565807484444456 5.0231845080971226E-5 74-75 3.0139107048582733E-4 2.5115922540485613E-5 0.0 3.5722502208945386 5.0231845080971226E-5 76-77 3.0139107048582733E-4 2.5115922540485613E-5 0.0 3.91935227040405 5.0231845080971226E-5 78-79 3.0139107048582733E-4 2.5115922540485613E-5 0.0 4.296656216768495 6.278980635121403E-5 80-81 3.7673883810728415E-4 2.5115922540485613E-5 0.0 4.700256534032828 7.534776762145683E-5 82-83 3.7673883810728415E-4 2.5115922540485613E-5 0.0 5.111793482819955 7.534776762145683E-5 84-85 3.89296799377527E-4 2.5115922540485613E-5 0.0 5.527160609794507 8.790572889169965E-5 86-87 4.018547606477698E-4 2.5115922540485613E-5 0.0 5.9639516186960915 1.0046369016194245E-4 88-89 4.018547606477698E-4 2.5115922540485613E-5 0.0 6.427503643064565 1.0046369016194245E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5800 0.0 39.7672 1 GTATCAA 9180 0.0 38.490837 1 ATCAACG 11480 0.0 30.241106 3 TCAACGC 11765 0.0 29.58938 4 TATCAAC 11805 0.0 29.569693 2 CAACGCA 11930 0.0 29.06055 5 AACGCAG 12520 0.0 27.82167 6 GTACATG 11535 0.0 24.94302 1 TACATGG 11705 0.0 23.971615 2 ACGCAGA 14350 0.0 23.910011 7 CGCAGAG 14650 0.0 23.225758 8 ACATGGG 12385 0.0 21.964268 3 GCAGAGT 15925 0.0 20.708952 9 GAGTACT 7955 0.0 20.12909 12-13 CATGGGG 8480 0.0 19.348171 4 GGACCGA 1355 0.0 19.288876 6 GTACTTT 8450 0.0 18.77864 14-15 AGTACTT 8500 0.0 18.586908 12-13 AGAGTAC 14390 0.0 18.558725 10-11 GTATAGG 780 0.0 18.29102 1 >>END_MODULE