FastQCFastQC Report
Thu 26 May 2016
SRR938599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3981538
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67480.16948224530319692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55140.13848919688823766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60800.040.1770131
GTATCAA93050.036.313771
ATCAACG118850.028.0161063
TCAACGC121500.027.4441474
CAACGCA123200.027.0654535
TATCAAC123500.027.0524432
AACGCAG127650.026.1117576
GTACATG116050.024.0387041
TACATGG117350.022.8814472
ACGCAGA147300.022.435017
CGCAGAG152600.021.5935848
ACATGGG123050.021.4625873
GGACCGA14500.020.3022176
GAGTACT83100.019.48377812-13
GCAGAGT166950.019.2540479
AGTACTT88400.018.90645812-13
AGGACCG17400.018.8360025
GTATAGG7650.018.6370831
AGAGTAC150850.018.42898410-11
GTGGTAT35450.018.3663331
Produced by FastQC (version 0.11.5)